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129results about How to "High application potential" patented technology

Transcranial ultrasonic multi-frequency acoustic holographic lens design method and system

The invention discloses a transcranial ultrasonic multi-frequency acoustical holographic lens design method and system. The method comprises the following steps: setting a plurality of different focus points; using a k-wave toolbox in Matlab, respectively setting a plurality of different focus points as sound sources by using a time reversal method, respectively adopting a plurality of driving frequencies to carry out simulation experiments, capturing the phase of the plane where the ultrasonic transducer is placed, and obtaining a plurality of acoustic holograms; according to the method, effective information of a plurality of acoustical holograms is extracted and integrated into a multi-frequency acoustical hologram, and after a transcranial multi-frequency acoustical hologram is obtained, phase information of the multi-frequency acoustical hologram is extracted and converted into the height of each pixel of the acoustical hologram lens. Multi-frequency transcranial focusing based on the multi-frequency acoustical holographic lens is realized by extracting effective information of a plurality of acoustical holographic lenses which are focused at different positions and have different working frequencies and integrating the effective information into one multi-frequency acoustical holographic lens, and corresponding focuses are reconstructed on a focusing plane according to different driving frequencies, so that flexibility in transcranial focusing is realized, and the focusing precision is improved. The application potential is improved.
Owner:FUDAN UNIVERSITY

Slow-release antibacterial reinforced composite film for fresh keeping of fruits and vegetables and preparation method of slow-release antibacterial reinforced composite film

The invention provides a slow-release antibacterial enhanced composite film for fruit and vegetable preservation and a preparation method thereof, and the preparation method comprises the following steps: performing sulfonation modification treatment on nano cellulose powder to obtain sulfonated nano cellulose; carrying out quaternization modification treatment on the sulfonated nano-crystalline cellulose to obtain quaternary ammonium sulfonated nano-crystalline cellulose; adding gelatin and sodium carboxymethyl cellulose into deionized water to obtain a wall material solution, and adding curcumin into the wall material solution to obtain a prepolymer solution; stirring and emulsifying the prepolymer solution at a high speed to obtain a prepolymer suspension; adding a chitosan solution into the prepolymer suspension to obtain a microcapsule solution, adding tannic acid into the microcapsule solution to obtain a microcapsule suspension, and performing spray drying to obtain double-layer microcapsule powder; the preparation method comprises the following steps: uniformly mixing polylactic acid, quaternary ammonium sulfonated nanocellulose, the double-layer microcapsule powder, a plasticizer, a lubricant and an antioxidant to obtain a mixed material, and sequentially carrying out melt extrusion, cooling, pelletizing and film blowing.
Owner:中科纳美(天津)生物工程有限公司 +1

Method for reducing fishy smell in chlorella

The invention relates to the technical field of chlorella deodorization, in particular to a method for reducing fishy smell in chlorella. By regulating the concentration of inorganic selenium in the culture medium, growth of chlorella is not inhibited, selenium-enriched biological enhancement is realized, the content of typical marker hexanal with fishy smell and grass taste of chlorella is remarkably reduced, meanwhile, the content of methyl hexanoate with sweet fragrance and fruit fragrance is increased, and the content of chlorella is increased. The fishy smell reduction and aroma enhancement of the chlorella are fundamentally realized. According to the method, the flavor characteristic of the selenium-enriched chlorella is effectively improved, the application potential of the selenium-enriched chlorella in food and related fields is greatly promoted, and the selenium-enriched chlorella is easier to accept by consumers.
Owner:WUHAN POLYTECHNIC UNIVERSITY

Polychromatic light excitation and thermal excitation luminous rare earth doped fluorescent powder as well as preparation method and application thereof

The invention provides rare earth doped fluorescent powder capable of emitting light through polychromatic light excitation and thermal excitation and a preparation method and application of the rare earth doped fluorescent powder. The fluorescent powder comprises rare earth doped fluoride, the chemical formula of the rare earth doped fluoride is Cs2NaLnF6: xRe < 3 + >, wherein Ln represents a rare earth element, Re < 3 + > represents a doped rare earth ion, and Ln and Re < 3 + > are respectively selected from different rare earth elements; x is the doping amount of Re in each mole of rare earth doped fluoride, 0 lt; xlt; and 100 mmol%. According to the invention, the advantages of wide band gap and appropriate fluorine vacancy trap of the Cs2NaLnF6 material are utilized, and the trap distribution and luminescence performance of the material can be effectively regulated and controlled by doping different types of rare earth ions with different concentrations and contents; polychromatic light excitation and thermal excitation luminescence of one or more rare earth ions under different doping concentrations are realized, and the application potential is high. The Cs2NaLnF6 fluorescent powder composite film material disclosed by the invention is excellent in flexibility, good in water resistance, high in stability and simple in preparation process, and has a great application prospect in the fields of information storage, encryption, counterfeiting prevention and the like.
Owner:FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI

Bacillus thuringiensis ZR-560 and application thereof

PendingCN119265066AImprove insecticidal virulenceStrong stress resistanceBiocideBacteriaMicroorganismAureobasidium sp.
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus thuringiensis ZR-560 and application thereof, the bacillus thuringiensis ZR-560 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30586. The invention further discloses a preparation method of the bacillus thuringiensis ZR-560. The bacillus thuringiensis ZR-560 strain provided by the invention has high insecticidal toxicity, and the parasporal crystal protein preparation of the bacillus thuringiensis ZR-560 strain has a corrected death rate of 100% for spodoptera frugiperda, ostrinia nubilalis, prodenia litura and beet armyworm, and is a Bt strain with a relatively broad-spectrum insecticidal effect.
Owner:CHENGDU ZHIRUN AGRICULTURE CO LTD

Switch cabinet fault diagnosis method and device based on deep learning

The invention discloses a switch cabinet fault diagnosis method and device based on deep learning, and belongs to the technical field of power equipment. The method comprises the following steps: S1, acquiring PD data; s2, pre-processing the PD data; the preprocessing content comprises the following steps: performing global statistical feature extraction on the PD data to obtain global statistical features; carrying out data type conversion on the PD data to obtain a grayscale image; s3, processing the grayscale image by adopting a ResNet model combined with a CBAM attention mechanism, and extracting key potential features as key local discrimination features; s4, performing feature splicing on the global statistical features and the key local discrimination features to obtain multi-scale features; and S5, performing classification processing by using an MLP classifier to obtain a switch cabinet fault diagnosis result. According to the method, signal features can be analyzed from different levels and scales through multi-scale feature fusion, and the fault diagnosis precision and adaptability are improved.
Owner:NANKAI UNIV

Lithium lanthanum titanium oxide solid electrolyte material and preparation method thereof

The invention discloses a lithium lanthanum titanium oxide solid electrolyte material and a preparation method thereof, and belongs to the field of solid electrolyte materials. The lithium lanthanum titanium oxide solid electrolyte material is of a perovskite structure, and the chemical composition of the lithium lanthanum titanium oxide solid electrolyte material is Li3xLa2 / 3-xTiO3, wherein x is greater than or equal to 0.10 and less than or equal to 0.15; the relative density of a ceramic sample sintered at 900 DEG C is greater than 90%; and the total impedance of the ceramic is less than 95k omega. According to the invention, a new complexing agent (diethylenetriamine pentaacetic acid or a combination of ethylenediamine tetraacetic acid and diethylenetriamine pentaacetic acid) is adopted to replace a conventional complexing agent citric acid, the stability of the new complexing agent and metal ions is higher, the hydrolysis rate of metal cations is slowed down, the obtained powder is better in quality, and the service life of the powder is prolonged. After being sintered into the lithium lanthanum titanium oxide solid electrolyte material, the lithium lanthanum titanium oxide solid electrolyte material not only has higher density, but also reduces the grain impedance and grain boundary impedance of the solid electrolyte material, and improves the application potential of the lithium lanthanum titanium oxide solid electrolyte material in preparation of a solid lithium ion battery.
Owner:ZHEJIANG NARADA POWER SOURCE CO LTD +1

Preparation method of rod-like selenium nano-carrier

The invention discloses a preparation method of a rod-like selenium nano-carrier, and belongs to the technical field of nano-pesticides. The rod-like selenium nano-carrier is formed by converting chitosan modified amorphous selenium (a-Se) into single crystal ash selenium (t-Se). The amorphous selenium is initially spherical, and after chitosan modification, the spherical amorphous selenium is converted into single crystal ash rod-shaped selenium nanoparticles. The length of the rod-like selenium nano-carrier is 590-610 nm, the diameter of the rod-like selenium nano-carrier is 20-25 nm, the effective average particle size of the rod-like selenium nano-carrier is 90-110 nm, the drug loading rate is 39.28% + / -2%, and the encapsulation efficiency is 64.90% + / -3%. In addition, the invention also provides an application of the rod-like selenium nano-carrier in a nano bactericide material.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Large-specification TC4 titanium alloy bar for large aero-engine fan blisk and preparation method of large-specification TC4 titanium alloy bar

The invention discloses a large-specification TC4 titanium alloy bar for a large aero-engine fan blisk and a preparation method of the large-specification TC4 titanium alloy bar, a large TC4 titanium alloy cast ingot with the diameter phi of 860-1000 mm is used and subjected to upsetting, drawing and cogging by 2-3 heating numbers, and the deformation amount of each heating number is controlled to be 40-70% for sufficient deformation; the method comprises the following steps of: performing upsetting and drawing for 3-7 heating numbers, controlling the deformation amount of each heating number to be 40-95%, controlling the upsetting and drawing temperature to be performed in a beta-phase region and a two-phase region, and controlling the change of a cooling mode after upsetting and drawing, finally converting the structure form into an equiaxed structure, and increasing the primary alpha-phase content in the structure to 70-90%, thereby obtaining the large-specification TC4 titanium alloy bar with good strength and plasticity. And the diameter phi of the bar is 400-800 mm, and the bar can be directly used for manufacturing the blisk, so that the processing and application potential of the titanium alloy is further widened, and the application field of the titanium alloy is enriched.
Owner:BAOSHAN IRON & STEEL CO LTD +2

Octapeptide, preparation method and application of octapeptide in antibiosis or immunoregulation

The invention provides the immunomodulatory octapeptide, the small peptide molecule has a unique structure, high broad-spectrum antibacterial activity, strong pathogenic bacterium inhibition and high purity, the stability of the structure and the antibacterial activity can be maintained in artificial gastric juice, no obvious hemolytic activity exists, and the immunomodulatory octapeptide has a very high application value in livestock breeding. The invention also provides a preparation method and a purity detection method of the immunomodulatory octapeptide.
Owner:CHONGQING ACAD OF ANIMAL SCI +1

Annotation-free sign language translation method based on fusion attention

The invention discloses an unannotated sign language translation method based on fusion attention. The unannotated sign language translation method comprises the following steps: acquiring a sign language video and a sign language spoken language text corresponding to the sign language video; constructing an initial sign language translation model based on a Transform architecture, and training the initial sign language translation model only through a sign language video and a sign language spoken language text to obtain an unannotated sign language translation model; in the training process, internal attention of the pseudo annotations and attention between the pseudo annotations are calculated and fused, and meanwhile, a contrast loss combined cross entropy loss training model is introduced; and translating the sign language video to be translated through the sign language translation model to obtain spoken language text output. The effect is improved in the aspect of annotation-free sign language translation, and compared with a previous sign language translation method, the sign language translation method has higher accuracy and application potential.
Owner:LANZHOU UNIV +1

Multi-channel optical anti-counterfeiting pattern based on hierarchical structure as well as preparation method and application of multi-channel optical anti-counterfeiting pattern

PendingCN119101969AHigh precisionEfficient use ofShaping toolsStampsPeriodic nanostructuresEngineering
The invention belongs to the technical field of optical anti-counterfeiting, and particularly relates to a multi-channel optical anti-counterfeiting pattern based on a hierarchical structure and a preparation method and application of the multi-channel optical anti-counterfeiting pattern. The preparation method comprises the following steps: respectively prefabricating a nickel impression with a periodic nanostructure and a nickel impression with a periodic micron structure; and the aluminum base with the work hardening effect is sequentially impressed through the nanometer nickel impression die and the micron nickel impression die. Through the method, the multi-channel optical anti-counterfeiting pattern based on the hierarchical structure is successfully prepared. Due to the fact that the periodic nanometer structures and the periodic micrometer structures have independent and different diffraction peaks respectively, people can decode different encrypted pattern information at different observation angles, and special tools or extra environmental stimulation do not need to be used. The characteristic is expected to play a key role in information security fields such as anti-counterfeiting, information encryption and the like.
Owner:WESTLAKE UNIV

Hydrated boric acid ester micromolecule with boron-nitrogen coordination bond, derivative resin material and preparation method of hydrated boric acid ester micromolecule and derivative resin material

The invention relates to a hydrated borate micromolecule with a boron-nitrogen coordination bond, a derivative resin material and a preparation method of the hydrated borate micromolecule. The preparation method comprises the following steps: S1, synthesizing boron-nitrogen coordination micromolecules: dissolving and reacting boric acid, a derivative thereof and a polyfunctional group amine compound at room temperature through a solution reaction to obtain the boron-nitrogen coordination micromolecules; the borate micromolecule NCBW-X with intramolecular boron and nitrogen coordination is formed; s2, the NCBW-X micromolecule can be used for curing epoxy resin and synthesizing materials such as phenolic resin through end-NH2 and end-OH on two sides, the NCBW-X improves the mechanical property of each system through a rigid eight-membered ring, boron-nitrogen coordination of the NCBW-X can generate hydration, the environmental endurance is improved on the premise of retaining the original dynamic nature of boric acid ester of the NCBW-X, and the service life of the NCBW-X micromolecule is prolonged. Meanwhile, due to the dynamic reversibility of the NCBW-X, the NCBW-X is used for curing epoxy resin, and materials such as phenolic resin and the like are improved to a certain extent in the aspects of self-repairing and recycling.
Owner:HUANGOU NEW MATERIALS (CHONGQING) CO LTD

Unmanned aerial vehicle multi-order distribution carbon emission reduction benefit prediction method and system

The invention discloses an unmanned aerial vehicle multi-order distribution carbon emission reduction benefit prediction method and system, and relates to the technical field of carbon emission reduction benefit prediction, and the method comprises the steps: obtaining unmanned aerial vehicle distribution data, carrying out the processing of the unmanned aerial vehicle distribution data, separating an independent distribution order and an order-combined distribution order, and taking the separated distribution order and order-combined distribution order as order-combined distribution stroke data; variable features are constructed on the basis of the order-combining distribution travel data, feature variables of an unmanned aerial vehicle order-combining distribution travel emission reduction benefit prediction model are generated, and construction of the variable features comprises three levels of time, space and travel; the feature variables of the unmanned aerial vehicle order-combining distribution travel emission reduction benefit prediction model are input into a pre-established optimization prediction model, a carbon emission reduction benefit prediction result is obtained through output, and the pre-established optimization prediction model is obtained after key hyper-parameters are optimized through a Bayesian hyper-parameter optimization method by means of a gradient lifting model.
Owner:SOUTHEAST UNIV +1

Method for increasing squalene yield in schizochytrium limacinum fermentation process by using small-molecule additive and application of squalene yield in schizochytrium limacinum fermentation process

The invention belongs to the technical field of microbial fermentation, and discloses a method for increasing the squalene yield in the schizochytrium limacinum fermentation process through a small molecule additive and application of the squalene yield in the schizochytrium limacinum fermentation process. Transferring the obtained activated bacteria to a seed culture medium to obtain a first generation of seeds; transferring into a seed culture medium and culturing again to obtain second-generation seeds; transferring into a seed culture medium, and culturing again to obtain third-generation seeds; and transferring into a fermentation culture medium containing terbinafine for fermentation culture so as to generate squalene. According to the method, the yield of squalene produced by the schizochytrium limacinum is increased by adding small molecular substances into a schizochytrium limacinum fermentation system. A small amount of specific proportion of terbinafine can effectively inhibit squalene from being oxidized into sterol substances in the fermentation process of schizochytrium limacinum, so that the consumption of squalene is reduced. Compared with the method without adding the terbinafine, the method has the advantages that the content of squalene is 2.63 times of that of a control group by adding the terbinafine with proper concentration, and the ratio of the squalene to the biomass is 2 times of that of the control group.
Owner:NANJING NORMAL UNIVERSITY

Method for preparing sodium sulfate from copper smelting slag slow cooling circulating water and drying equipment

The invention provides a method for preparing sodium sulfate by using copper smelting slag slow-cooling circulating water and drying equipment, and the method comprises the following steps: S1, collecting arsenic-containing waste acid generated in a copper smelting process, and carrying out vulcanization and neutralization treatment processes to enable various indexes in the waste acid to reach a reuse standard; s2, the waste acid obtained in the step S1 is placed in a storage container, and solid particles and impurities in the waste acid are removed; s3, heating and evaporating the waste acid obtained in the step S2, and concentrating until no mother liquor exists; and S4, washing with a small amount of distilled water, carrying out centrifugal spin-drying, and drying in drying equipment to finally obtain dried sodium sulfate powder. By recovering and utilizing sodium sulfate in waste acid, reasonable utilization of resources is realized, and resource waste and environmental pollution are reduced.
Owner:CHIFENG JINJIAN COPPER IND CO LTD

Antibacterial cellulose modified polylactic acid and preparation method thereof

The invention relates to the technical field of high polymer materials, and discloses antibacterial cellulose modified polylactic acid, triazole compounds have excellent activity in the antifungal aspect, and sulfanilamide groups can influence nucleic acid metabolism of thalli and synthesis of dihydrofolic acid so as to play a bacteriostatic role. Under the synergistic effect of a sulfanilamide group and a triazole group, the synthesized antibacterial cellulose has excellent antibacterial activity, the antibacterial group is introduced in a grafting manner, so that the long-acting antibacterial activity can be maintained, the polylactic acid is modified by using the cellulose, and due to the large length-diameter ratio of the cellulose and mutual overlapping of the cellulose, the antibacterial activity of the polylactic acid is improved. A good net structure is formed in a polylactic acid matrix through hydrogen bonds and Van der Waals force, so that a toughening effect on polylactic acid can be achieved, and the modified polylactic acid not only has excellent antibacterial activity, but also has better mechanical properties and thermal stability.
Owner:CHANGCHUN EXPRESS NETWORK TECHNOLOGY CO LTD

Image comparison test method

The invention belongs to the technical field of image processing, and discloses an image comparison test method, which specifically comprises the following steps: step 1, image input and processing: a user needs to firstly input an image needing to be compared, and provides a comparison parameter; and 2, image validity verification: before comparison, the application program carries out image validity verification. The method has the advantages that the accuracy and reliability of image similarity comparison are improved, and the conditions of errors and missing detection in practical application are avoided; the application range is wide, and the method can be used for comparison of various image types; the method is widely applied to the field of digital media, and can be used for video coding, image compression, image denoising and the like; image quality evaluation and optimization are supported, the method can be used for image similarity comparison, and the SSIM comparison method can also be used for image quality evaluation and optimization, so that the image is more vivid and real; the method can be applied to image search, image recognition and the like to carry out accurate search and recognition.
Owner:HAINAN CHEYOUJIA INFORMATION TECH CO LTD

Control method and device of rotating structure, equipment and medium

ActiveCN119126601APrecise and intelligent operationAccurate understanding of intentProgramme control in sequence/logic controllersControl engineeringHuman–computer interaction
The invention relates to the technical field of intelligent control, in particular to a rotating structure control method, device and equipment and a medium, and the method comprises the steps: obtaining the sound information and somatosensory information of a user in a current site in real time, and setting an initial monitoring area; acquiring user quantity information in the current site, setting a user tag for each user and associating the user tags; analyzing expression information included in the sound information of each user and setting a corresponding weight coefficient; dividing the initial monitoring area into a plurality of sub-areas and adjusting the size and position of the initial monitoring area; extracting specific instruction information in the sound information and the somatosensory information, obtaining a corresponding instruction action in the specific instruction information, and associating the instruction action with a corresponding user tag; and based on the weight coefficient and the instruction action, controlling the rotating structure to execute the corresponding action. The specific instruction information can be extracted in real time, and the control strategy can be dynamically adjusted, so that the operation of the rotating structure is more accurate and intelligent, and the overall user experience is optimized.
Owner:DONGGUANSHIXINGHUO GEARS CO LTD

Plasma arc additive manufacturing device and method based on wire powder coaxiality

The invention discloses a plasma arc additive manufacturing device and method based on wire and powder coaxiality, and belongs to the field of arc additive manufacturing. The device comprises a multi-wire powder coaxial welding gun; the welding gun comprises a copper nozzle, a welding gun shell and a powder feeding shell, an ion gas port is formed in the middle of the copper nozzle, wire feeding ports are formed in the front end and the rear end of an ion gas outlet, and powder feeding ports are formed in the left end and the right end of the ion gas outlet; the wire powder coaxial copper nozzle is located at the bottom end of the plasma arc welding gun and connected with the welding gun shell. A second channel between the ion gas shell and the powder feeding shell is used for feeding powder; through holes are formed in the surfaces of the two sides of the welding gun shell and the powder feeding shell correspondingly and used for feeding welding wires, and the welding wires are fed into the wire feeding opening through the second channel. According to the device, the wire feeding channel and the powder feeding channel are coaxially integrated, and metal wires and metal powder are ensured to be melted and deposited in the plasma arc center by accurately controlling the conveying paths of the two materials.
Owner:HARBIN ENG UNIV

Goat embryonic stem cell culture system and establishment method and application thereof

The invention relates to the technical field of cell biology, and particularly discloses a goat embryonic stem cell culture system, a system establishment method and application, the goat embryonic stem cell culture system takes mTeSRTM Plus as a basic culture solution, and 20 ng / mL to 25 ng / mL of leukemia inhibition factors, 1 mu M to 1.5 mu M of PD0325901, 4 mu M to 5 mu M of IWR-1, 45 mu g / mL to 50 mu g / mL of Vc, 15 ng / mL to 20 ng / mL of activin A, 1 mu M to 1.2 mu M of CGP77675 and 1.5 mu M to 2 mu M of GO6983 are added. Cell clone with clear edges is obtained through culture of a goat embryonic stem cell culture system, stable passage can be achieved, and the cell clone has the potential of being differentiated into three germ layers.
Owner:NORTHWEST A & F UNIV

Ferroelectric monolayer vanadium trichloride film and preparation method thereof

ActiveCN116411340BBreaking the spatial inversion symmetryhigh application potentialPolycrystalline material growthAfter-treatment detailsSemiconductor materialsScanning tunneling microscope
The application relates to a ferroelectric monolayer vanadium trichloride film and a preparation method thereof. The method comprises the following steps: evaporating and depositing VCl3 powder on the surface of a NbSe2 substrate under an ultrahigh vacuum atmosphere, and performing annealing treatment, so that the ferroelectric monolayer vanadium trichloride film is obtained. The sample preparation strategy of molecular beam epitaxy under an ultrahigh vacuum environment is used, high-purity VCl3 powder is evaporated and deposited on a NbSe2 substrate, an atomically flat monolayer VCl3 sample is obtained through annealing treatment, in-situ scanning tunneling microscope technology and scanning tunneling spectrum technology are combined, and it is determined that the monolayer VCl3 is a semiconductor material with ferroelectricity. The method provides a new method and idea for introducing ferroelectricity in the family of transition metal trichlorides, has high application potential and scientific research value, meanwhile, the successful introduction of ferroelectricity in the system also provides a new platform for carrying out multi-state regulation and research.
Owner:WUHAN UNIV

Simulation method and device for harmonic detection of interface pressure of cable joint, and medium

The invention provides a simulation method and device for detecting interface pressure of a cable joint through harmonic waves and a medium. The method comprises the following steps: modeling a simulation geometric model for the cable intermediate joint, and setting an ultrasonic excitation source; modeling through a nonlinear spring damper to obtain a cable joint pressure model; and performing nonlinear ultrasonic detection cable joint interface pressure simulation on the cable joint pressure model to obtain a spectrogram at each point probe. According to the invention, on a cable joint model, modeling is carried out through a nonlinear spring damper, nonlinear interaction at a cable intermediate joint interface is simulated, a nonlinear harmonic detection principle is utilized, and the change of harmonic characteristics when the interface pressure changes is deeply explored, so that the relationship between the two is established; the states of the cable intermediate joint under various interface pressures are accurately simulated, the interface pressure of the cable intermediate joint is fully considered, and simulation analysis is performed on the interface pressure detection of the cable joint by establishing a new interface nonlinear model.
Owner:CHINA ELECTRIC POWER RESEARCH INSTITUTE CO LTD +2

A highly accurate base editor that expands editing range and improves editing efficiency

The present invention relates to the field of genetic engineering in biotechnology, and specifically to a high-precision base editor that expands the editing range and improves editing efficiency. The editor of the present invention constructs a CDA1Δ-SpRY-BE3 high-precision editor with a wider editing range and higher editing efficiency by fusing the Cas9 variant SpRY that is not restricted by PAM with the cytosine deaminase CDA1 of different truncation lengths. The editor can effectively edit any cytosine on the genome while maintaining high editing accuracy. Tests in yeast and rice cells showed that compared with the existing standard editor CDA1-SpRY-BE3, the editor of the present invention achieved a significant improvement in editing efficiency at multiple targets, proving its application value in higher eukaryotes. The present invention provides a more efficient and accurate tool for the treatment of genetic diseases and crop breeding.
Owner:SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY +1

Wild vegetable natural composite preservative and preparation method thereof

The invention belongs to the field of agriculture, and particularly relates to a wild vegetable natural composite preservative and a preparation method thereof, the wild vegetable natural composite preservative is prepared from the following raw materials by weight: 0.6-0.9 part of a mixed flavone extract, 1-2 parts of L-malic acid, 2-3 parts of sodium lactate, 10-20 parts of a glucose solution, and 20-30 parts of glycerin. The natural composite preservative is prepared by adopting a method for extracting flavone from plants, and the prepared product is healthy and safe to use and has excellent preservative and fresh-keeping effects. L-malic acid is used as a pH regulator, so that the pH value of the preservative can be maintained in a proper range, the growth of microorganisms and the activity of enzymes are inhibited, and the preservation effect is further improved.
Owner:昆明理工大学津桥学院 +1

Multiphase electrolyte for membrane-free single-liquid single-tank structure zinc-bromine flow battery

The invention relates to the technical field of electrochemical energy storage, in particular to an electrolyte for a zinc-bromine flow battery with a membrane-free single-liquid single-tank structure. The electrolyte is a multiphase mixed solution, wherein a light phase component mainly comprises an aqueous solution of zinc bromide, zinc chloride and 1-butyl-2-methyl pyridine bromide; and the heavy phase component of the electrolyte is mainly a bromine complex of 1-butyl-2-methyl brominated pyridine. The invention has the following remarkable beneficial effects: firstly, the zinc-bromine flow battery can normally operate under a membrane-free single electrolyte storage tank structure, so that the battery framework is simplified, and the initial installation cost is reduced; and secondly, the problems of bromine cross diffusion, zinc dendrite and the like of the membrane-free structure zinc-bromine flow battery are effectively solved by a plurality of trace special additives, the battery is ensured to run well under the membrane-free structure, and the battery performance is ensured while the cost is reduced.
Owner:SOUTH CHINA UNIV OF TECH +1

Cathode structure with high porosity and high electro-catalytic performance and preparation method thereof

PendingCN119243230Amaintain long-term stabilitymaintain structural integrityElectrodesAl powderAlloy coating
The invention discloses a high-porosity and high-electrocatalytic-performance cathode structure and a preparation method thereof.The preparation method comprises the following steps that S1, Ni powder and Al powder in a certain proportion are mixed to be uniform, the mixture is sprayed to the surface of a nickel net through a plasma spraying method, and a NiAl alloy coating is obtained; s2, Al elements in the NiAl alloy coating are removed through a chemical activation treatment method, and a porous nickel structure with high porosity is formed; s3, performing pretreatment operation to remove oil stains and oxides on the surface of the porous nickel structure; and S4, depositing a Mn-doped ZnCoP transition metal phosphide coating by adopting a two-step constant-potential electro-deposition method to obtain the cathode structure. According to the preparation method of the cathode structure, batch preparation can be achieved, the cost advantage is high, the prepared cathode structure has high porosity and high electro-catalytic performance, high stability under the harsh condition is achieved, and the long-term operation requirement for efficient water electrolysis hydrogen production can be met.
Owner:NINGBO UNIV

Intelligent mechanical response light-emitting heterojunction material and preparation method and application thereof

The invention provides an intelligent mechanical response luminescent heterojunction material and a preparation method and application thereof, the chemical expression is x ZnS-(1-x) CaZnSO: 1% Mn < 2 + >, 0 < = x < = 1; the preparation method of the material comprises the following steps: (1) mixing ZnS, CaCO3 and MnCO3 according to a molar ratio of (1: 0)-(1: 1), and then adding MnCO3 accounting for 1% of the mass of the mixed powder; adding ethanol, grinding, and uniformly mixing to obtain mixed powder; (2) putting the mixed powder into a raw material reaction bottle, then putting the raw material reaction bottle into a reactor, and preparing a heat-insulating brick group to obtain a reaction device equipped with the mixed powder; and (3) preparing a microwave reaction furnace provided with a turntable capable of controlling rotation, placing the reaction device in the microwave reaction furnace for a synthetic reaction, and cooling after the reaction is finished to obtain the intelligent mechanical response luminescent heterojunction material. The material disclosed by the invention is obvious in mechanical luminescence phenomenon and high in luminescence efficiency, can directly generate the mechanical luminescence phenomenon through external force application, and can be applied to preparation of a mechanical force responsive film.
Owner:JIANGNAN UNIV

Anti-jumping system based on Wi-Fi signal and multiple sensing

PendingCN119136160AAvoid issues such as privacy violationsAvoid Privacy ConcernsMeasurement devicesParticular environment based servicesSensing dataData acquisition
The invention discloses an anti-drop system based on a Wi-Fi signal and multiple sensors. The anti-drop system comprises a data acquisition module, a data processing module, a data identification module, a multi-modal data decision fusion module and a protection measure module. The data acquisition module is used for acquiring CSI data and physical sensing data; the data processing module is used for preprocessing the CSI data and the physical sensing data; the data identification module classifies and identifies the preprocessed CSI data and physical sensing data to obtain classification and identification results; and the multi-modal data decision fusion module performs decision-level fusion by using logic and operation according to the classification and recognition result to obtain a decision-making result, and starts different levels of protection measures according to the decision-making result. According to the invention, dangerous behavior actions possibly causing the jumping can be detected, corresponding protection measures can be started, early prevention and early response can be realized, and meanwhile, the method has the advantages of high accuracy and sensitive response.
Owner:ZHEJIANG UNIV OF SCI & TECH

Virus nucleic acid detection reagent, kit, preparation method and application thereof

The preparation method of the viral nucleic acid detection reagent provided in the present application, after the ASFV amplicon hybridizes with crRNA, Cas12a cuts MB-ssDNA-ALP to release ALP, and the released ALP catalyzes the hydrolysis of pNPP into pNP, thereby generating a colorimetric signal, and the inner filtration effect of pNP on CdZnSe quantum dots changes the fluorescence signal, and the ASFV gene is detected using this dual-mode method, and the absorption intensity or fluorescence intensity is well linear with the ASFV gene concentration, and the detection limit is a single copy. In addition, the method can detect single-copy ASFV genes with the naked eye, and the detection sensitivity is higher than that of qPCR. Finally, the method can also effectively distinguish single-base mutations in crRNA, and has a good detection effect on viral DNA in serum. Therefore, the method has good sensitivity and specificity, and has a high application potential in the diagnosis of viral infections.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI