316results about How to "High application potential" patented technology

The invention discloses a ZIF-8 encapsulated amino ionic liquid type carbon dioxide adsorbing-catalyzing agent. The ZIF-8 encapsulated amino ionic liquid type carbon dioxide adsorbing-catalyzing agent is prepared according to the following steps: taking the metal organic framework material ZIF-8 as a carrier; taking the amino-group functionalized ionic liquid 1-(3-aminopropyl)-3-methylimidazole chloride as an active component; immobilizing the amino-group functionalized ionic liquid in the super-cages of the ZIF-8 carrier in an in-situ manner during preparation of ZIF-8, wherein 1.5 to 5 mmol of the amino-group functionalized ionic liquid is immobilized in each gram of the adsorbing-catalyzing agent. The adsorbing-catalyzing agent integrates the functions of selective adsorbing and catalyzing into a whole, and can be used for synchronously adsorbing and converting carbon dioxide, so as to reduce the cost of the CUU technology.

The invention provides a method for recycling uranium in uranium-contained waste water. The method is characterized by recycling and enriching the uranium in the waste water through the steps of ammonia removal of the waste water as well as pretreatment of organisms, uranium adsorption by chromatographic columns, scouring by acid solution and deposition. The method is high in recycling efficiency, low in cost, free from producing secondary waste liquid, compact in technological flow, applicable to the treatment of different uranium-contained waste liquid and high in practicability.

The invention discloses a cyclodextrin glucosyl transferase enzyme mutant, and belongs to the field of genetic engineering and enzyme engineering. The cyclodextrin glucosyl transferase enzyme is mutated to obtain a mutant having higher dismutation activity than that of the cyclodextrin glucosyl transferase enzyme. The shake-flask fermentation enzyme dismutation activities of mutants V6D, S90G, T168A, T171A, T383A, G608A and V6D / S90G / T168A / T171A / T383A / G608A are respectively 1.89, 1.21, 1.21, 1.22, 1.32, 2.03 and 3.16 times that of wild enzyme. The mutant has certain magnificence for industrialproduction of cyclodextrin glucosyl transferase enzyme, and the application potential of the enzyme in foods, medicine and chemical engineering industries can be improved.

The invention discloses a metal-sulfur battery based on sulfur-rich transition metal sulfide and a preparation method thereof. The metal-sulfur battery comprises a cathode, an anode and an electrolyte; the anode is metal; the cathode comprises transition metal sulfide; a chemical formula of the transition metal sulfide is MSx, wherein x is more than or equal to 3; M is one or more of vanadium, niobium, titanium, molybdenum, tungsten, iron, cobalt and nickel; the metal is lithium, sodium, potassium, magnesium, aluminum, zinc or iron; the electrolyte is an ester-based electrolyte or an ether-based electrolyte. The metal-sulfur battery based on the sulfur-rich transition metal sulfide and the preparation method thereof, disclosed by the invention, have the benefits that transition metal polysulfide is adopted as a cathode material equivalent to sulfur so as to realize the novel metal-sulfur battery such as a lithium sulfur and sodium sulfur battery system, and further the application potential of the metal-sulfur battery is further improved.

The invention discloses a polymer based nanocomposite and a preparing method thereof. According to the invention, an organic covalent modification technology is adopted, polyhydroxy cyclodextrin molecules are anchored on a graphene oxide nanosheet through a coupling agent, excellent dispersion in various solvents is realized through utilizing the hydroxyl on the cyclodextrin to simulate the characteristic that the graphene oxide is enriched with oxygen species, the capacity of cyclodextrin on the graphene oxide is controlled through adjusting the reaction temperature, further the solvent dispersing performance and thermal stability of functionalized hybrid materials can be effectively regulated and controlled; besides, the solvent blending technology is adopted, the cyclodextrin functionalized grapheme based nano hybrid materials are used as fillers to prepare different-polymer based nanocomposite; through the adoption of the method, the intermolecular and intramolecular interfacial interaction are further enhanced so as to improve the thermal stability of the functionalized nano materials and polymer based nanocomposite adopting the nano materials as fillers, besides, the synthetic steps are simple and efficient, the aftertreatment is simple and concise, and large-scale preparation is facilitated.

The invention relates to a colloidal gold test strip for semi-quantitative detection of progesterone and a preparation method thereof. The invention belongs to the technical field of dairy cow pregnancy diagnosis. Colloidal gold test strip for semi-quantitative detection of progesterone, the sample pad, the gold pad containing gold-labeled progesterone antibody complex, and the nitrocellulose membrane are pasted end to end on the bottom plate; there are detection lines and control lines on the nitrocellulose membrane . Preparation of test strips: preparation of colloidal gold: prepared from chloroauric acid and trisodium citrate; preparation of purified polyclonal antibody: preparation of artificial antigen of dairy cow progesterone by dicyclohexylcarbodiimide coupling method; artificial antigen of progesterone was then used Immune mice to prepare progesterone polyclonal antibody; prepare gold-labeled antibody: colloidal gold and antibody adsorption, centrifugal purification; colloidal gold test strip preparation: soak gold-labeled pad with Tween-containing PB solution, gold-labeled antibody perfusion glass fiber Membrane; nitrocellulose membrane Spray bovine progesterone artificial antigen and goat anti-mouse secondary antibody into 2 lines with a film dispenser. The invention is simple in operation, convenient and practical, efficient and fast, and easy to popularize.

The invention discloses a preparation method for a photonic crystal structure film electrode of a dye solar cell in the technical field of solar cells. A photonic crystal structure positive template structure is introduced into the solar cell and is packaged combined with a pyridine complex dye of ruthenium so as to form the photonic crystal dye solar cell with improved efficiency. A photonic crystal structure positive template is prepared through emulsifier-free emulsion polymerization and a vertical deposition method, and a titanium dioxide photonic crystal reverse template is prepared through a sandwich treatment topology, thereby improving an electrode structure of the traditional dye cell, exceeding the limitation of laboratory photo-etching processing and enhancing the application potential thereof. The prepared photonic crystal structure film electrode takes a three-dimension ordered anatase type titanium dioxide hole arranged by an inverse opal structure as the electrode of the dye solar cell, the hole diameter of film materials thereof ranges from 50nm to 500nm, and the thickness ranges from 0.5 micron to 40 microns, thereby enlarging the specific surface area combined with the dye and having higher short circuit current and filling factors.

The invention relates to the field of gene engineering, and in particular relates to a lipase, gene and application thereof. The lipase has an amino acid sequence shown as a SEQ ID NO.1. The invention aims to solve the defects of an existing technology, and clones a lipase gene from Aspergillus niger. High expression of the lipase in Pichia pastoris reaches a level higher than that of the existing technique. Therefore, the recombinant lipase provided by the invention can greatly reduce production cost in industrial production, so as to show great application potentials in industries such as washing, papermaking and food.

The invention discloses an adsorbing-catalyzing agent based on amino-functionalized ionic liquid. The adsorbing-catalyzing agent is prepared by taking a mesoporous moecular sieve SBA-16 as a carrier and amino-functionalized ionic liquid 1-(3-aminopropyl)-3-[3-(triethoxysilyl)propyl]-4,5-dihydroimidazole chloride as an active component and grafting the amino-functionalized ionic liquid to the carrier, wherein 1-10 mmol of the amino-functionalized ionic liquid is loaded in every 1 g of the adsorbing-catalyzing agent. The adsorbing-catalyzing agent integrates an adsorption capturing function and a catalytic conversion function into a whole and can achieve synchronous adsorption and conversion of CO2 and reduce the cost of a CUU technique.

The invention discloses a partial discharge direction finding method based on an ultrahigh-frequency phased array principle in a substation. The method includes following steps: (1) acquiring and recording partial discharge signals in the substation by employing the ultrahigh-frequency phased array and a digital detection device with high sampling rate; (2) focusing the partial discharge signals to form narrowband signals at a reference frequency point by employing a broadband focusing algorithm; (3) performing direction-of-arrival estimation on the narrowband signals by employing a narrowband direction finding algorithm; and (4) obtaining the point with maximum azimuth density with combination of multiple direction-finding results, namely the azimuth of the partial discharge signals. According to the method, the size of the ultrahigh-frequency phased array is small so that tour inspection is facilitated, and the spatial resolution is high; and according to the method, for the partial discharge signals in the substation, the partial discharge signals are converted to the narrowband signals by employing the focusing algorithm, and the azimuth of the partial discharge signals is obtained by employing the direction finding algorithm so that the direction-finding precision is high, and the ability of suppressing noise interference is great.

The invention provides a composite nanofiltration membrane adopting tannic acid-titanium as an isolation layer and a preparation method of the composite nanofiltration membrane, which relates to the technical field of membranes. The preparation method of the composite nanofiltration membrane adopting tannic acid-titanium as the isolation layer comprises the following steps: soaking a basic membrane in an aqueous solution containing tannic acid; and adding a metal titanium salt, in-situ growing a tannic acid-titanium cross-linked functional surface layer on the basic film, and obtaining the composite nanofiltration membrane. The preparation method is simple in operation, mild in conditions, low in cost, and environmentally friendly. The composite nanofiltration membrane adopting the tannicacid-titanium as the isolation layer is prepared by adopting the method. The composite nanofiltration membrane has excellent isolation function, anti-pollution performance and antibacterial activity.

The invention discloses a preparation method of low-molecular-weight sea cucumber fucosan sulfate. In the preparation method, a sea cucumber polysaccharide prepared by performing enzymolysis on pulverized sea cucumbers is used as a raw material. The preparation method comprises the following steps: 1) dissolving the sea cucumber polysaccharide in a sodium chloride solution, then adding ethanol, and centrifuging to remove the precipitate, thus obtaining the supernatant; and adding ethanol into the supernatant, centrifuging, dialyzing the obtained precipitate, and performing freeze-drying to obtain sea cucumber fucosan sulfate; 2) dissolving the sea cucumber fucosan sulfate in solvent, and performing ultrasonic treatment at 0-45 DEG C, wherein the treatment time is 10-180 minutes, and the ultrasonic sound field strength is 60-800 W / cm<2>; and 3) dialyzing the treated solution obtained in the step 2), and performing vacuum freeze-drying to obtain the low-molecular-weight sea cucumber fucosan sulfate. According to the invention, an ultrasonic degradation technology is adopted, high-molecular-weight sea cucumber fucosan sulfate can be quickly degraded, the reaction conditions are mild, and the preparation method is green and environment-friendly.

The invention discloses temperature-sensitive polymer, a synthesis method thereof and temperature-sensitive injectable hydrogel. A temperature-sensitive polymer is obtained by synthesizing GelMA and UPyMA, dispersing UPyMA, GelMA and MEOnMA monomers in an alkaline water solution of 8-11 in pH, removing oxygen, adding an oxidative reduction initiator or a water soluble initiator at room temperaturefor polymerization reaction until reaction is sufficient, purifying a product, and removing a solvent to obtain the temperature-sensitive polymer. The temperature-sensitive polymer can be prepared into injectable aqueous-phase temperature-sensitive polymer sol when being not higher than corresponding low critical phase transition temperature and not lower than water freezing point in a normal-pressure environment, and the sol can immediately form gel when being higher than the corresponding low critical phase transition temperature. The temperature-sensitive injectable hydrogel has dynamic mechanical performance and high biocompatibility and can be applied in the field of biomedicine like tissue engineering, drug controlled release and cell three-dimensional culture.

The invention relates to the field of gene engineering and particularly provides belta-mannase which is derived from Aspergillus niger and has the collection of 4235 in the CGMCC and the amino acid sequence of SEQ ID NO.1. The invention also provides a gene of coding the belta-mannase, wherein the sequence of the gene is shown as SEQ ID NO.2. The optimal temperature of the mannose is 75 DEG C; the optimal pH value is 3.0; and the belta-mannose is acidic. Therefore, the anti-nutritional effect of the mannase is reduced effectively in an acidic environment of animal intestines and stomach, and the utilization rate of feed by the animals is increased. The invention also provides a method of effectively expressing the gene of the belta-mannase. The expressing quantity can achieve 11,785U / mL. Compared with the prior art, the invention ensures that expressing quantity of the belta-mannase is higher. Therefore, the industrial production cost can be largely reduced and the belta-mannase has greater application potential in fields of feed, food, petrochemical industry, brewing and the like.

The invention provides a star polymer as well as a preparation method and application thereof. The preparation method of the star polymer comprises the following steps: S1, mixing pentaerythritol with2-bromoisobutyryl bromide to obtain a star initiator; S2, carrying out mixed reaction on the star initiator with a nitrogenous acrylate monomer. Compared with exogenous nucleic acid with high preparation cost or nucleic acid molecules with low delivery efficiency, the star polymer provided by the invention has good water solubility and low cytotoxicity; the preparation method of the star polymeris simple and the preparation cost is low; the star polymer and exogenous nucleic acid molecules can form a stable compound which quickly enters cells.

The invention discloses a flame-retardant elastic nanocellulose aerogel which is prepared from reactive organic compound and polybasic carboxylic acid-containing flame-retardant system by using biomass raw materials of the cellulose. The invention further discloses a preparation method of the aerogel. Compared with the traditional nanocellulose aerogel, the prepared aerogel has good flame-retardant performance, as well as excellent resilience performance, and the application potential and usage safety of the material in the fields of heat insulating field and the like can be greatly improved.

The invention discloses bacillus amyloliquefaciens RL263 capable of preventing and controlling rice blast, as well as an inoculant containing the bacillus amyloliquefaciens RL263, a method for preventing and controlling rice blast by using the bacillus amyloliquefaciens RL263 and application of the bacillus amyloliquefaciens RL263 in prevention and control of rice blast. The bacillus amyloliquefaciens has an effective prevention and control effect on rice blast.

A wolfram steel powder high-density material and a method of hot extrusion for producing the material belong to metal composite material and producing method. The method solves the problem that the cost of equipment used in present method is high; the technical process is complicated and the density is a bit low; the energy consumption is great and the production efficiency is low; the density is low with the melt infiltration method and the combination of two metals is not good. The wolfram steel powder high-density material is made according to the weight percent that wolfram accounts for 50 to 90 percent and copper accounts for 10 to 50 percent. The producing method is that wolfram and copper powders are taken as the raw materials; a mechanical ball is used to grind the raw material; the process of cold-press shaping is implemented; the material is put in a steel sleeve and the sleeve is welded after the sleeve being sealed; the material is heated and put into a mold cavity; the material is extruded with a squeeze head and the high-density material will be obtained. The present invention solves the problem that with the traditional method of sintering and infiltration and hot isostatic method, the wolfram and copper material density is low and the two materials can not melted together and the problem that the producing method for regular deformed composite material adopts great extrusion ratio which is hard to be applied in industry.

A method for forming a conductive film at room temperature is provided and includes steps of: adding AgNO3 into a first solution of dodecanoic acid; dropping n-butylamine and a diluted aqueous solution of a reducing agent into the first solution in turn, so as to initially obtain the dodecanoate-protected silver nanoparticles as a capping ligand; then using cyclohexane as a solvent to apply the silver nanoparticles onto a surface of a substrate to form a patterned film of silver nanoparticles; and finally immersing the substrate into a high concentrated aqueous solution of a reducing agent to chemically reduce the patterned film of silver nanoparticles into a conductive silver film. Thus, a patterned film or circuit can be conveniently and rapidly formed, and the silver nanoparticles can be applied to flexible substrates with low material cost and temperature sensitivity.

The invention provides a targeted antibacterial peptide against Gram-negative bacteria and a preparation method and an application. A sequence of the targeted antibacterial peptide is as shown in a sequence table SEQ ID No. 1, and in the preparation method, an R language is utilized for statistical analysis of amino acid sequence characteristics of a natural antimicrobial peptide library, variouspeptide chain parameters affecting antibacterial activity of a peptide chain having the antibacterial activity only against Gram-negative bacteria are statistically analyzed, and the optimal amino acid composition is obtained by screening, namely K, G, L, with the number of positive charges being 4, and the hydrophobicity being 30%-50%. Tryptophan is inserted into the center of a screening sequence to obtain a centrally symmetric short peptide sequence through design, so as to obtain a short-chain narrow-spectrum antibacterial peptide sequence which is low in toxicity and highly effective, andnot easy to cause disorder of immune regulation in a body. The targeted antibacterial peptide is beneficial to maintaining micro-ecological balance while killing harmful bacteria. The antibacterial peptide has relatively high activity, high stability, relatively low cytotoxicity, and substitute antibiotics, thereby having an application potential for becoming a safer and environmentally-friendlynovel antibacterial product.

The invention relates to a hydrogen-doped inlet air and oxygen-enriched gasoline engine with EGR and a combustion control method. The device mainly comprises a gasoline engine, an EGR cooler, an EGR valve, a water electrolysis device, a hydrogen storage, an oxygen storage, a pressure control device, a hydrogen injection device and an oxygen injection device. According to the hydrogen-doped inlet air and oxygen-enriched gasoline engine, in-cylinder combustion modes of four air inlet modes including inlet air doped with hydrogen and oxygen, small-scale exhaust gas recirculation and hydrogen doping, large-scale exhaust gas recirculation and hydrogen doping and oxygen enrichment are respectively adopted in a cold start and idling condition, a medium or small load condition, a large load condition and a full load condition of the engine, so that the effects that the thermal efficiency is obviously improved and the HC and CO discharge is reduced at the cold start and idling stage of the gasoline engine can be achieved; the pumping loss of the engine is greatly reduced in the medium or small load condition, and the oil consumption and NOx discharge are greatly reduced; the engine power is obviously increased within short time when a throttle valve is fully opened. The hydrogen-doped inlet air and oxygen-enriched gasoline engine has excellent overall properties such as low emission, low oil consumption and instantaneous high power output.

The invention discloses a paenibacillus fermentation broth and a preparation method and application thereof. The fermentation broth is fermented supernatant obtained through fermental cultivation of paenibacillus CGMCC No.8333. It is proven through tests that the paenibacillus fermentation broth has an effect of promoting growth of lactobacillus plantarum ST-III. The method for preparing the fermentation broth is simple, the equipment requirements are low, and the cost is low. The adopted paenibacillus CGMCC No.8333 comes from foods and is safe and non-toxic, and the supernatant prepared through fermentation can be directly applied to food processing. Besides, the fermentation broth greatly improves application potential of lactobacillus plantarum ST-III in actual product development.

The invention discloses a method for processing paper-making waste water in the way of structuring artificial flora, including selecting more than two strains of Halomonas sp. flora and Bacillus sp. flora respectively for sterile culture to lead OD620nm to reach over 10.0 and then mixing the bacterial liquid by volume rate to structure the artificial flora; inoculating the paper-making waste water of pH being 9-11 and COD being 1,000-140,000mg l <-1> with the structured artificial flora, conducting stationary treatment for a certain time at 35-38 DEG C with intermittent stirring, in the treatment process, sampling at intervals to measure the pH, chroma and COD index of the paper-making waste water and finishing the treatment when the pH reduces to 7.0-8.8. The method is characterized by simple structure of the flora to be structured, obvious effect in direct treatment, strong controllability and repeatability and the like, and has great application prospect in the treatment aspects of waster water such as the control over the paper-making waste water.

The invention provides novel rail type fire rescue equipment which comprises a fire rescue platform guard bar, a winding engine, a vehicle-mounted hydraulic lifting supporting plate, a hanging cabin, a wall body, a sliding wheel support, a hanging cabin sliding wheel, a rack, a gear, a fire rescue platform and the like. Half H-shaped steel height of a guide rail is embedded into the high-rise building wall body to serve as a guide device. The fire rescue platform is connected with the sliding wheel through the sliding wheel support and is matched with the guide device guide rail, and thus lifting motion is conducted through meshing of the gear with the rack on the guide rail. The fire rescue platform is composed of a top fire protection region and a lift car region. The winding engine provides power for the hanging cabin, and the hanging cabin is connected with the fire rescue platform through the hanging cabin sliding wheel and connected with the sliding wheel through the sliding wheel support and makes contact with the guide device guide rail to finish the lifting motion. The rail type fire rescue equipment has the advantages of being fast and convenient to use, high in rigidity and the like, plays a significant role in high-rise or super-high-rise building fire rescue process and is higher in applicability, higher in practicability and good in development prospect.

The invention discloses a quick induced spore production method and application of Phomopsis, belonging to the field of phytopathogen induced spore production. According to the method, Phomopsis mycelia cultured by a PDA (potato dextrose agar) solid culture medium, which is used as a spore production base, is subjected to lawn brooming, sterilized water flushing, drying and constant-temperature lighting culture to induce the mycelia to quickly produce abundant conidiomata within 4 days. Nine different strains of Phomopsis are respectively utilized to quickly produce spores in the mycelia in the dryly-stored activated state. When being used for quick simple induced spore production of Phomopsis, the method can greatly enhance the spore production efficiency, shortens the identification period, effectively saves the human and material resources and the abundant precious time of researchers, and thus, has great application potential and higher economic value.

The invention discloses portable no-key-hole friction stir spot welding equipment with a self-holding function. The equipment mainly consists of a power motor, a transmission mechanism, a welding mechanism, a servo mechanism, a brake, a shell and an armrest frame. The transmission mechanism consists of a shaft gear pair and a main shaft, and is connected with the power motor through a sleeve coupling. The servo mechanism consists of a servo motor and a spiral transmission device; the transmission mechanism is associated with the servo mechanism through an angular contact ball bearing; and the tail end is connected with the welding mechanism through a thread. The equipment is high in flexibility and portability, has the self-holding function in the welding process to deal with the welding demands of various workpiece shapes and various welding positions, largely reduces the requirements on operator physical ability and operation technology, improves the equipment welding stability, and facilitates to improve the welding quality and the production efficiency.

The present invention discloses a cyclodextrin glucosyltransferase mutant T168A and a preparation method thereof, and belongs to the field of gene engineering and enzyme engineering. Cyclodextrin glucosyltransferase is mutated to obtain the mutant with the higher cyclodextrin glucosyltransferase disproportionation activity. The shake-flask fermentation enzyme disproportionation activity of the mutant T168A is 1.21 times that of a wild enzyme. The mutant has a certain significance for industrial production of cyclodextrin glucosyltransferase, and improves the application potential of the cyclodextrin glucosyltransferase in the food, medicine and chemical industries.

The invention relates to a preparation method of cationic nanometer cellulose containing lignin, and belongs to the technical field of cellulose modification. The preparation method comprises following steps: agricultural waste is crushed, and is mixed with an alkali solution and a cationic etherifying agent, microwave and supersonic wave combined one-kettle method is adopted for reaction, and cellulose nanometer modification is carried out, so that the cationic nanometer cellulose containing lignin is prepared through one-kettle method. The preparation method is simple; reaction temperature is reduced; reaction time is shortened; industrialized production is possible to be realized; at the same time, the obtained cationic nanometer cellulose containing lignin is high in stability, film forming strength, and antibacterial performance, excellent effect can be achieved with the cationic nanometer cellulose containing lignin is used for heavy metal absorption in water, and the applicationvalue of the cationic nanometer cellulose containing lignin in the fields of antibacterial and absorption is extremely high.

The invention discloses a surface-modified heterogeneous knot titanium dioxide photonic crystal thin film catalyst. The catalyst is of a three-dimensional ordered inverse opal structure and has an anatase / rutile heterogeneous knot crystal structure, and a photonic band gap is located at a visible light absorption region of the catalyst. The invention further discloses a preparation method of the surface-modified heterogeneous knot titanium dioxide photonic crystal thin film catalyst. The preparation method comprises the following steps: preparing TiO2 sol by virtue of an in-situ modification method; preparing polystyrene microsphere colloidal crystal template by virtue of a vertical deposition method; preparing a nano-TiO2 photonic crystal with the three-dimensional ordered inverse opal structure by virtue of a dipping-vertical pulling method; and carrying out high-temperature calcination, so as to obtain the surface-modified heterogeneous knot titanium dioxide photonic crystal thin film catalyst. The catalyst has very strong visible-light responsivity, and the visible light chemical reaction is enhanced by virtue of a slow photon effect of the photonic crystal; by utilizing the heterogeneous knot structure, the separation of photon-generated current carriers is promoted, and the visible light catalysis capacity is improved; and furthermore, equipment is simple and easy to operate.