77results about How to "Does not inhibit growth" patented technology

The invention relates to a probiotic complex against helicobacter pylori and a preparation method of the probiotic complex. The probiotic complex is prepared from the following raw materials: 2 to 10parts by weight of lactobacillus helveticus, 10 to 25 parts by weight of bifidobacterium animalis, 5 to 15 parts by weight of bifidobacterium bifidum, 10 to 25 parts by weight of lactobacillus acidophilus, 2 to 15 parts by weight of lactobacillus rhamnosus, 1 to 6 parts by weight of bifidobacterium bifidum, 5 to 15 parts by weight of soy protein powder, 50 to 100 parts by weight of inulin, 20 to 50 parts by weight of resistant dextrin, 20 to 50 parts by weight of fructose-oligosaccharide, 2 to 5 parts by weight of flos lonicerae water extract and 10 to 20 parts by weight of prunus armeniaca fruit. The complex can achieve the purpose of eradicating the helicobacter pylori; in addition, the complex has the advantages of being free of side effects, fast in effect taking, thorough in treatmentin the use process and free of recurrence in a year after treatment.

The invention discloses a photosynthetic bacteria culture medium, relates to a bacteria culture medium, and solves the problems that the existing photosynthetic bacteria culture media contains high-concentration yeast extract that leads to darker lysate color and high production cost, and pH value of the culture medium rises along with the prolonging of cultivation time, thus inhibiting the growth of photosynthetic bacteria and leading to lower bacteria concentration. The photosynthetic bacteria culture medium is made of low molecular weight carbonaceous organic substances, ammonium salts, the yeast extract, MgSO4, CaCl2, K2HPO4, KH2PO4, trace elements and distilled water. As the photosynthetic bacteria culture medium takes the low molecular weight carbonaceous organic substances that do not contain Na<+> as carbon source, pH value of the culture medium does not rise along with the prolonging of cultivation time, and the growth of photosynthetic bacteria is not inhibited; low-concentration yeast extract is adopted to reduce production cost, and the trace elements ensure bright photosynthetic bacteria lysate color; photosynthetic bacteria cultivated by the culture medium has the exponential growth phase of two days, thus having high bacteria concentration and the OD660 of 1.8.

The invention discloses a removing method of hexavalent chromium in the effluent in the anaerobic/aerobic batched reactor through active sludge microbe in the environment protective technical domain, which comprises the following steps: placing active sludge in the SBR; adding effluent with Cr (VI) into the reactor; stirring 1-6h under anaerobic condition; aerating for 1-6h under aerobic condition; sedimenting 1-2h; making the density of hexavalent chromium and total chromium in the outlet supernatant reach the first grade national discharge standard; reducing the harm of environment and human due to chromium; enriching trivalent chromium in the sludge with low toxicity; operating the chromium removing system continuously without inhibiting the growth of microbe.

The invention relates to a nifuratel-nystatin soft capsule-type suppository and a preparation method thereof. Nifuratel-nystatin sol in the nifuratel-nystatin soft capsule-type suppository is preparedfrom the following components in parts by weight: 40-60 parts of nifuratel, 3-5 parts of nystatin, 2-3 parts of carbomer, 1-2 parts of ethylparaben, 50-70 parts of glycerinum, 70-100 parts of ethyl alcohol, 20-30 parts of pH buffer liquid and 700-800 parts of medical pure water. The preparation method comprises the following steps of 1, raw material preparation; 2, liquid medicine preparation, wherein the nifuratel and the nystatin are mixed evenly in a sterile environment, then through a sterile filtering system, sterile filtering is conducted, and then micro-pore filtering is conducted, sothat a liquid medicine is obtained for later use; 3, gel matrix preparation; 4, nifuratel-nystatin sol preparation; 5, capsule pressing; 6, soft capsule-type suppository preparation. The nifuratel-nystatin soft capsule-type suppository and the preparation method thereof have the advantages that body hormone secretion is not affected, the sterilization effect is good, and the preparation method issimple.

Relating to the technical field of microorganisms, the invention provides a bacillus coagulans MES847, a microbial inoculum, a chicken feed, a preparation method and application. The bacillus coagulans MES847 provided by the invention is preserved in China General Microbiological Culture Collection Center on August 30, 2018, and the preservation number is CGMCC NO.16358. The bacillus coagulans MES847 has a wide antibacterial spectrum, can be used for preparation of products preventing and/or treating chicken diarrhea, at the same time can improve the cellular immunity and humoral immunity level of organisms, stimulates the development of animal immune organs, and improve the disease resistance of animals.

The invention relates to an active component baicalin of a Qingkailing compound, which has an inhibiting effect on acinetobacter calcoaceticus, acinetobacter baumannii, A.baumannii, stenotrophomonas maltophilia and escherichia coli containing NDM-1 drug resistance gene, so that the active component baicalin can be used for inhibiting these bacterial diseases and treating and/or preventing diseases caused by these bacteria.

The invention provides a fig leaf extract with bacterium colony induction quenching activity, and a use thereof, and concretely relates to a preparation method of a fig leaf crude extract, a bacterium colony induction quenching activity screening method, and an application of the extract in the preparation of bacterium colony induction inhibitors. The bacterium colony induction quenching activity of the fig leaf extract is reported for the first time in the invention. The fig leaf crude extract prepared through extraction using organic solvents with different polarities has an inhibition effect on Chromobacterium violaceum and Pseudomonas aeruginosa colony induction system, wherein a dichloromethane extract has strongest colony induction system quenching activity. The crude extract can substantially reduce the output of colony induction mediated Chromobacterium violaceum purpurin and the flocking motion of the Pseudomonas aeruginosa and weaken the pesticide resistance of pathogenic bacteria, and can be used to prepare novel bacterium colony induction inhibitors, so the fig leaf extract has wide application prospect in prevention and control of bacterial infective diseases.

The invention discloses a method for increasing the content of flavonoids in hydroponic lettuce in a greenhouse by adding UV-B radiation. The method comprises the steps of taking lettuce seeds, conducting disinfecting and placing the seeds on gauze infiltrated with a 1/2 Hoagland nutrient solution; placing the gauze and lettuce seeds in a dish, sealing the dish with a plastic wrap with holes, placing the dish in an environment with a temperature of 15-20 DEG C and white light to conduct cultivation until germination, continuing conducting cultivation for 5-7 days, then conducting hydroponics by a 1/2 Hoagland nutrient solution for 2 to 3 weeks, then adding UV-B radiation to the lettuce seedlings for 3 to 4 hours per day, repeating the UV-B radiation operation for 6 to 8 days, and then harvesting lettuce. The method of the invention can significantly improve and stably maintain the content of flavonoids in the hydroponic lettuce in the greenhouse, wherein the flavonoid content increasesby 30% or more after 6 days of UV-B radiation, the nutritional quality of the lettuce is increased, and growth of the lettuce is not inhibited, and the organism amount is not affected.

The invention provides an application of pyrimidine compounds in the promotion of metabolite synthesis and the hormone level in rice. The pyrimidine compounds are 6-(methoxymethyl)-2-[5- (trifluoromethyl)-2-pyridyl]pyrimidin-4-ol. The compounds can obviously rapidly improve the pathogen invasion resistance of plants, does not inhibit the growth of the plants or the growth of a root system, and also promotes the synthesis of the metabolites and the increase of the hormone content in the rice.

The present invention discloses an application of flavone oxygen glycoside compounds in preparations of bacterial quorum sensing inhibitory drugs. Structure of the compounds is as described in a formula I; wherein R1 is H, hydroxy or methoxy; R2 is hydroxy; R3 is H, hydroxy or galactoside; and R4 is hydroxy or galactoside. The present invention provides a novel use of the flavone oxygen glycosidein preparations of the bacterial quorum sensing inhibitory drugs. Experimental results show that the flavone oxygen glycoside can all inhibit biofilms of pseudomonas aeruginosa 9027, pseudomonas aeruginosa 27853 and drug resistance pseudomonas aeruginosa, show that the flavone oxygen glycoside compounds can inhibit the bacterial quorum sensing system to reduce bacterial virulence and pathogenicity, while bacterial growth is not inhabited and bacteria are not easy to produce the drug resistance of the bacteria, it is indicated that the flavone oxygen glycoside compounds can be prepared into a pseudomonas aeruginosa quorum sensing inhibitor.

The invention discloses N-15 marked microcystis and a production method thereof. The production method comprises the following steps of microcystis aeruginosa cleaning: using the microcystis aeruginosa in a logarithmic phase as microcystis aeruginosa seeds, performing suspension cleaning on the microcystis aeruginosa seeds by deionized water, and collecting microcystis aeruginosa bodies after centrifugation, wherein the number of cleaning times is at least two; microcystis aeruginosa culture: inoculating the cleanly cleaned microcystis aeruginosa into a special culture medium with N-15, performing culture in light (the light intensity is about 2500 to 5000LUX, and the temperature is 25 to 28 DEG C), shaking up a culture bottle for 1 to 5 times every day, and performing culture for 10 to 30days to obtain culture liquid; and microcystis extraction: performing lysis treatment on microcystis aeruginosa cells in the culture liquid, performing centrifugation, and collecting liquid supernatant to obtain microcystis crude extraction liquid. The production method is simple; the operation is easy; the N-15 marking rate of the prepared microcystis is high; when the prepared microcystis is used as a standard product, a matrix effect is reduced; and the quantification is more accurate. Compared with the existing non-standard microcystis, better application prospects are realized.

The invention discloses a pear fruit calyx-eliminating flower-thinning disease-preventing agent as well as an application method and application of the pear fruit calyx-eliminating flower-thinning disease-preventing agent. The calyx-eliminating flower-thinning disease-preventing agent comprises the following components: 15-25mg/L flusilazole and 45-55mg/L 6-benzyl purine. Compared with the prior art, the calyx-eliminating flower-thinning disease-preventing agent can not degrade the fruit quality and inhibit tree growth on the basis that the calyx-eliminating rate of pear fruits is improved, and meanwhile as the flusilazole has the function of fungus control, the calyx-eliminating flower-thinning disease-preventing agent further has the functions of preventing pear tree ring spot, rot, scab and anthracnose.

The invention relates to a microbial agent for preventing and treating the white stool syndrome of litopenaeus vannamei as well as a preparation method and application of the microbial agent. The microbial agent is fermented powder prepared by performing spray drying on fermentation liquid of enterococcus faecalis. The preparation method of the microbial agent for preventing and treating the whitestool syndrome of the litopenaeus vannamei comprises the following steps: sequentially performing shake-flask culture, first-stage seed culture, second-stage seed culture and fermentation culture onthe enterococcus faecalis, and then drying to obtain the microbial agent for preventing and treating the white stool syndrome of the litopenaeus vannamei. In the application of the microbial agent forpreventing and treating the white stool syndrome of the litopenaeus vannamei, the microbial agent is blended into feed in proportion, and then the feed is used for feeding the litopenaeus vannamei ina pond in which white stool is formed for treatment or not formed for prevention. The microbial enterococcus faecalis agent can significantly inhibit growth and reproduction of pathogens such as vibrio cholerae, edwardsiella tarda and aeromonas hydrophila, has broad-spectrum bacteriostasis, can prevent and treat the white stool disease of the litopenaeus vannamei, has the preventing and treatingeffect of 78% or above, and has a significant effect.

A method for removing bacteria of chlamydomonas reinhardtii through kresoxim-methyl, ampicillin and cefotaxime mixed antibacterial agents comprises the following steps: inoculating mixed and pollutedchlamydomonas through a TAP solid culture medium flat plate, and culturing; preparing a mixed antibacterial agent flat plate which is a TAP solid culture medium in which kresoxim-methyl, ampicillin and cefotaxime are added, and waiting for later use; transferring and marking the mixed and polluted chlamydomonas which is cultured on the TAP solid culture medium to the antibacterial agent mixed TAPsolid culture medium flat plate for culturing; and transferring and marking the antibacterial chlamydomonas from the antibacterial flat plate to a common TAP solid culture medium flat plate to cultureagain. According to the method, kresoxim-methyl, ampicillin and cefotaxime are used in match to effectively remove the bacteria and fungus pollution in the chlamydomonas culture process; the method has the characteristics of being high in wide popularization scope, efficient, and low in toxicity, and is extremely high in value of popularization and application in storing of precious chlamydomonasand accuracy improving of following experiments.

The invention provides a triterpenoid-saponin compound, wherein the molecular formula is C52H84O21, and the chemical name is 3beta-O-{beta-D- xylopyranosyl-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-[beta-D-glucopyranosyl(1->4)]-alpha-L-arabinopyranosyl}hederagenin. In-vitro anti-tumor tests indicate that the compound has a remarkable inhibiting action for C6 rat glioblastoma cells as well as four kinds of U87MG, U251MG, BT325 and SHG44 glioblastoma cells without influencing the growth of primarily-cultured human neuroglia cells. The compound is hopeful to be used for preparing a medicament for treating a glioma.

The invention discloses a gynecological disinfectant and a preparation method thereof, and belongs to the field of medical disinfectants. The gynecological disinfectant comprises the following components in percentage by mass: 0%-5% of glycerol, 0%-5% of polyethylene glycol-400, 0%-5% of tween-20, 0%-5% of tween-40, 8%-10% of vitamin C, 0%-2% of vitamin E, 0.4%-2% of folic acid, 0.5%-2% of acetate, 2%-8% of acetic acid and the balance of water. The gynecological disinfectant can be obtained by adding and uniformly mixing the components according to a certain sequence. The gynecological disinfectant has a broad-spectrum bactericidal effect on staphylococcus aureus, escherichia coli, candida albicans and the like which are common in gynecological vaginitis, cannot kill beneficial bacteria such as lactic acid bacteria and the like and can regulate and stabilize pH of vagina, the acting time is long, all the components are non-toxic, harmless and non-irritant, and the gynecological disinfectant has mild effects and is quite suitable for being used as a safe and effective compound disinfectant for treating vaginitis.

The invention relates to the technical field of industrial cultivation of edible fungi. More specifically, the invention discloses a method for sterilizing a liquid culture medium of an edible fungus strain. The method comprises the following steps: in an aseptic environment, mixing slightly acidic electrolyzed water with the liquid culture medium of the edible fungus strain according to a volume ratio of (1-2):20, shaking uniformly, and sealing the mixture to achieve an effect of killing heterogeneous bacteria. In comparison with a conventional high-temperature high-pressure sterilization method, the method disclosed by the invention not only saves water, electricity, and time costs, but also reduces the risk caused by the conventional method. Moreover, in comparison with reported sterilization by using slightly acidic electrolyzed water, the method disclosed by the invention does not inhibit the growth of the edible fungus strain while killing the heterogeneous bacteria, improves the strain inoculation efficiency, and meanwhile, a pungent odor generated by high-concentration active chlorine contained in strong acid water is avoided from causing adverse impact on the health of workshop staffs.