Application of pyrimidine compounds in promotion of metabolite synthesis and hormone level in rice

A technology of compounds and metabolites, applied in the fields of application, pest control, animal repellent, etc., can solve problems such as loss, ecological and social adverse effects, mutation resistance, etc.

Active Publication Date: 2018-06-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, breeding for disease resistance can easily lead to variation in physiological races of pathogenic bacteria, resulting in the loss of resistance of disease-resistant rice varieties within 3-5 years. Similarly, the extensive use

Method used

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  • Application of pyrimidine compounds in promotion of metabolite synthesis and hormone level in rice
  • Application of pyrimidine compounds in promotion of metabolite synthesis and hormone level in rice
  • Application of pyrimidine compounds in promotion of metabolite synthesis and hormone level in rice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Experimental material and method

[0029] 1 Plant materials and strains

[0030] The plant material used in the experiment was Nipponbare rice (Oryza sativa L.japonica.cv.Nipponbare).

[0031] The bacterial strain used in the experiment was Xanthomonas oryzae pv. oryzae (Xoo) GD-IV strain, all of which were obtained commercially.

[0032] 2 Experimental reagents and solution preparation

[0033] The preparation method of BTH mother liquor: Weigh a certain amount of BTH solid powder, add pure acetone to ultrasonically dissolve the mother liquor to a concentration of 100mM (mmol / L), store it in a refrigerator at 4°C, and keep away from light (wrapped in tinfoil).

[0034] Preparation method of SPB mother liquor: Weigh a certain amount of SPB powder, add ultrapure water to stir or sonicate until the solids are completely dissolved, make a mother liquor with a concentration of 1mM, and store it in the dark at 4°C.

[0035] Solid culture medium for bacterial ...

Embodiment 2

[0112] Embodiment 2 The effect of compound SPB of the present invention in the interaction between rice and pathogenic bacteria

[0113] Choose 300μM (μmol / L) as the concentration of traditional plant activator BTH. Three concentrations of 10 μM, 50 μM, and 100 μM were selected for gradient screening to ensure that they did not harm the plants themselves while inducing plant resistance. The BTH mother liquor is prepared with acetone (Acetone) as a solvent, and the SPB is directly dissolved in water. Therefore, the control for BTH was the 0.3% acetone-treated group, while the control for SPB was H 2 O treatment group. The rice plants were pretreated with phytoactivators by spraying on the surface of leaves. 0.3% Acetone, 300 μM BTH, H 2 O, 10 μM SPB, 50 μM SPB, 100 μM SPB, observe the plant phenotype after 5 days. Such as figure 1 As shown, there is no significant difference between the rice leaves of the above-mentioned plant activator treatment groups at various concent...

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Abstract

The invention provides an application of pyrimidine compounds in the promotion of metabolite synthesis and the hormone level in rice. The pyrimidine compounds are 6-(methoxymethyl)-2-[5- (trifluoromethyl)-2-pyridyl]pyrimidin-4-ol. The compounds can obviously rapidly improve the pathogen invasion resistance of plants, does not inhibit the growth of the plants or the growth of a root system, and also promotes the synthesis of the metabolites and the increase of the hormone content in the rice.

Description

technical field [0001] The invention belongs to the field of biological pesticides, in particular to a plant activator for inducing plant resistance. Background technique [0002] Plant pathogens and pests affect the potential yield and quality of crops worldwide. It is estimated that pests and pathogens reduce crop production by 20%-40% based on current crop protection methods. With the occurrence and intensification of plant diseases and insect pests, the production and consumption of pesticides are increasing year by year, which greatly promotes the fertility of agriculture. Traditionally, the control of agricultural pathogens and pests is often based on the synthesis of organic insecticides. Pesticides have the advantages of increasing production, saving labor, and reducing the risk of using chemical fertilizers, but they also bring about a series of problems, such as increased resistance of plant pathogens, environmental pollution, excessive pesticide residues, and th...

Claims

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Application Information

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IPC IPC(8): A01N43/54A01N25/02A01P21/00
CPCA01N25/02A01N43/54
Inventor 姚楠李俭龙婷尹健戴光义
Owner SUN YAT SEN UNIV
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