Application of pyrimidine compounds in promotion of metabolite synthesis and hormone level in rice
A technology of compounds and metabolites, applied in the fields of application, pest control, animal repellent, etc., can solve problems such as loss, ecological and social adverse effects, mutation resistance, etc.
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Embodiment 1
[0028] Embodiment 1 Experimental material and method
[0029] 1 Plant materials and strains
[0030] The plant material used in the experiment was Nipponbare rice (Oryza sativa L.japonica.cv.Nipponbare).
[0031] The bacterial strain used in the experiment was Xanthomonas oryzae pv. oryzae (Xoo) GD-IV strain, all of which were obtained commercially.
[0032] 2 Experimental reagents and solution preparation
[0033] The preparation method of BTH mother liquor: Weigh a certain amount of BTH solid powder, add pure acetone to ultrasonically dissolve the mother liquor to a concentration of 100mM (mmol / L), store it in a refrigerator at 4°C, and keep away from light (wrapped in tinfoil).
[0034] Preparation method of SPB mother liquor: Weigh a certain amount of SPB powder, add ultrapure water to stir or sonicate until the solids are completely dissolved, make a mother liquor with a concentration of 1mM, and store it in the dark at 4°C.
[0035] Solid culture medium for bacterial ...
Embodiment 2
[0112] Embodiment 2 The effect of compound SPB of the present invention in the interaction between rice and pathogenic bacteria
[0113] Choose 300μM (μmol / L) as the concentration of traditional plant activator BTH. Three concentrations of 10 μM, 50 μM, and 100 μM were selected for gradient screening to ensure that they did not harm the plants themselves while inducing plant resistance. The BTH mother liquor is prepared with acetone (Acetone) as a solvent, and the SPB is directly dissolved in water. Therefore, the control for BTH was the 0.3% acetone-treated group, while the control for SPB was H 2 O treatment group. The rice plants were pretreated with phytoactivators by spraying on the surface of leaves. 0.3% Acetone, 300 μM BTH, H 2 O, 10 μM SPB, 50 μM SPB, 100 μM SPB, observe the plant phenotype after 5 days. Such as figure 1 As shown, there is no significant difference between the rice leaves of the above-mentioned plant activator treatment groups at various concent...
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