28 results about "Xanthomonas" patented technology

The invention relates to the technical field of mineral processing, and particularly provides a combined reagent system for flotation and magnesium reduction of talc-containing copper-molybdenum ore and application of the combined reagent system. The combined reagent system comprises a flotation collecting agent (faint yellow to orange transparent liquid, 40%-50% of active ingredients and less than or equal to 3% of free alkali) with active ingredients in the formula 1 and a flotation depressing agent (light yellow powder, extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of 1000-1500000 Daltons and rich in hydroxyl, carboxyl and pyruvic acid groups) with a structural formula in the formula 2, and the flotation depressing agent comprises extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of xanthomonas and the weight-average molecular weight of xanthomonas and is rich in hydroxyl, carboxyl and pyruvic acid groups. Through the synergistic adsorption effect of the combined reagent, the flotation grade and the recovery rate of the copper sulfide molybdenum ore are remarkably improved under the condition that the pH is 8-11. The combined reagent system has the advantages of being high in flotation efficiency, high in selectivity, low in reagent cost, environmentally friendly, high in industrial applicability and the like.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention belongs to the technical field of genetic engineering, and particularly relates to application of a negative regulation XC1141 gene in improving the yield of xanthan gum produced by xanthomonas campestris var. Campestris. According to the invention, an Xcc 8004 genome is subjected to knockout modification, and a gene XC1141 in the Xcc 8004 genome is deleted, so that an Xcc 8004 mutant strain (mutant strain Xcc 1141) with high yield of xanthan gum is obtained; the xanthan gum yield of the obtained Xcc 8004 mutant strain can reach 9.11 g / L, and the yield is increased by 65% compared with that of wild fungus Xcc 8004 (5.52 g / L).

The invention provides a carpet grass xanthomonas phage, a compound phage preparation and application of the carpet grass xanthomonas phage and the compound phage preparation. The carpet grass xanthomonas phage is carpet grass xanthomonas phage XAC17P1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241062. The compound bacteriophage preparation provided by the invention has higher sterilization efficiency and better application stability, belongs to a green and efficient plant disease biological prevention and control means, and can effectively prevent and treat citrus canker.

The invention belongs to the technical field of gene engineering, and particularly relates to a preparation method and application of an engineering strain NK-02 for xanthan gum production. The preparation method of the engineering strain for xanthan gum production comprises the following steps: taking xanthomonas campestris with a xanthan gum production function as a basic strain, and knocking out a gumF gene, a gumG gene and a gumL gene in the basic strain; inserting a synthetic biological element fimE-CI857; and introducing an overexpression plasmid to obtain the xanthan gum production engineering strain. According to the preparation method of the engineering strain for xanthan gum production, xanthan gum with different properties can be produced by controlling the fermentation temperature of the engineering strain, and an effective way is provided for providing a controllable xanthan gum production method.

This invention discloses an R-type α-fluoroimine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from *Xanthomonas aeruginosa*. Luteolibacter luteus By using directed evolution to modify its active site, an imine reductase mutant with high activity, stereoselectivity, and high yield of 4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline was obtained. The mutation is at least one of S237T, I241L, or R248L. This invention achieves efficient synthesis of (R)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline from inexpensive (Z)-2,2,2-trifluoro-N-(4-methoxyphenyl)-1-phenylethylimine, which is beneficial for the industrial synthesis and application of (R)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline.

This invention belongs to the field of genetic engineering and biotechnology, specifically relating to a β-glucosidase PO3 for the conversion of rare ginsenosides, its preparation method, and its applications. This invention obtains β-glucosidase PO3 by PCR amplification using genomic DNA of *Xanthomonas pseudoepiplo* TY3-10 as a template. The results of the examples show that this enzyme can not only convert total ginsenosides into rare ginsenosides F2, C-O, C-Mx1, and C-Mc1, but also convert ginsenoside Rb1 into F2, ginsenoside Rb2 into C-O, ginsenoside Rb3 into C-Mx1, and ginsenoside Rc into C-Mc1; it also exhibits excellent catalytic performance and high expression efficiency, possessing extremely high application value and commercial prospects.

This invention discloses a strain of *Flavobacterium huinanense*, an inoculant, and its application in the control of potato scab, belonging to the field of green control technology for potato scab. This invention isolates a strain of *Flavobacterium huinanense* from the soil surface of potatoes. Flavobacterium anhuiense R10, which has a significant antagonistic effect on *Streptomyces bozzolani*, the pathogen of potato scab. *Flavobacterium huinanense* ( Flavobacterium anhuiense The fermentation broth of R10, when used for root irrigation of potatoes, can significantly reduce the incidence and severity index of potato scab.

The invention relates to the technical field of new strains for degrading cellulose, in particular to a pseudoxanthomonas strain JC1303 and application thereof. Through whole genome sequencing and function annotation, it is systematically revealed for the first time that the strain carries a complete cellulose degrading enzyme system including incision beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and can be used for degrading cellulose. And specific gene resources are determined through generic genome analysis. The strain has the remarkable technical effects that the strain shows higher cellulase activity after being cultured for 5 days, and cellulose materials such as agricultural wastes and the like can be stably and efficiently degraded in a salt-containing environment by virtue of the unique genetic background and marine source characteristics of the strain.

The structural formula of the thymol-containing quaternary ammonium salt derivative is shown as (1), wherein R1 is 2-isopropyl-5-methylphenyl, ethyl or a benzene ring, R2 is 2-chloro-5-methylthiophene, 3-methyl-2-methoxycarbonyl furan, 4-methylnaphthalene and differently substituted benzyl, X-is Cl-and Br-, and n is equal to 3, 5, 7, 8 and 9. The preparation method of the drug-loaded antibacterial liposome comprises the step of jointly assembling hydrogenated soybean phosphatidylcholine, cholesterol hemisuccinic acid monoester, phosphatidyl ethanolamine and a target compound thymol-containing quaternary ammonium salt derivative into the drug-loaded antibacterial liposome. Part of the compounds have excellent antibacterial activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri and Xanthomonas syringae pv. Kiwifruit. Meanwhile, the compound also has excellent biological activity on six fungi such as fusarium oxysporum and TMV viruses. A field test on Xoo shows that the protection effect of the drug-loaded antibacterial liposome is improved by 1.94 times, the treatment effect is improved by 1.56 times, and the drug-loaded antibacterial liposome shows remarkable pH-triggered release characteristics and colloidal stability. (1)

The invention discloses wide-temperature-range low-temperature beta-galactosidase OUC-JST derived from flavobacterium fusiformis as well as a coding gene and application of the wide-temperature-range low-temperature beta-galactosidase OUC-JST, and belongs to the technical field of functional enzymes. The beta-galactosidase OUC-JST has an amino acid sequence as shown in SEQ ID No.1, the optimum reaction temperature is 20 DEG C, the relative enzyme activity in a wide temperature range from 5 DEG C to 30 DEG C is kept at 50% or above, 20% of the enzyme activity is still kept at 0 DEG C, and the beta-galactosidase OUC-JST shows excellent wide-temperature-range low-temperature adaptability. The invention also provides a gene SEQ ID No.2 for coding the enzyme, a recombinant expression vector and recombinant engineering bacteria containing the gene, and a preparation method of the enzyme. The beta-galactosidase OUC-JST disclosed by the invention is suitable for hydrolyzing lactose at low temperature, and has a wide application prospect in the field of lactose-free dairy product production.

This invention discloses a metal ion-driven meliosteum peptide nanoassembly, its preparation method, and its applications, relating to the field of agricultural biotechnology. The meliosteum peptide nanoassembly is formed by the self-assembly of a hexahistidine-tagged meliosteum peptide with divalent metal ions through coordination. The average particle size of the meliosteum peptide nanoassembly is 20 nm–30 nm. The meliosteum peptide nanoassembly obtained by this invention through the self-assembly of a hexahistidine-tagged meliosteum peptide with zinc ions exhibits excellent antibacterial, anti-biofilm activity, stability, and biocompatibility, and can be used to control plant bacterial diseases caused by Xanthomonas and other fungi.

This invention discloses a novel salt-tolerant fungal strain, *Luteimonas sp.* R10, its prepared microbial agent, and its applications. The strain was isolated from the rhizosphere soil of cotton in saline-alkali land in Xinjiang. The microbial agent was obtained through fermentation. Treatment of wheat seeds with the *Luteimonas sp.* R10 microbial agent provided by this invention significantly improved wheat germination rate, budding rate, plant height, chlorophyll content, and phenylalanine hydrolase activity, increasing these by 26.67%, 21.28%, 41.34%, 98.73%, and 61.48%, respectively, compared to the control. When applied to cotton seed soaking, the cotton germination rate, plant height, and fresh weight increased by 200.00% compared to the control. 、 The concentrations of 31.50% and 9.37% indicate that the microbial inoculant prepared from the salt-tolerant new bacterium Luteimonas sp. R10 provided by this invention has a significant promoting effect on plant growth and early maturity, can increase plant disease resistance, and has good application prospects as a bio-fertilizer.

The invention discloses xanthomonas for promoting plant growth and resisting salt stress and application of the xanthomonas. The invention provides Xanthomonas sontii, the strain number of the Xanthomonas sontii is JT6-1, and the registration number of the Xanthomonas sontii in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.34844. The Xanthomonas sontii JT6-1 strain provided by the invention has salt-tolerant and drought-resistant capabilities, and in combination with pot experiment results, the JT6-1 strain shows good growth-promoting characteristics under salt stress, so that the application potential of the JT6-1 strain in improvement of plant stress resistance is further proved, the JT6-1 strain not only can be used as the JT6-1 strain, but also can be used as the JT6-1 strain, the JT6-1 strain and the JT6-1 strain, and the JT6-1 strain and the JT6-1 strain can be used for improving plant stress resistance. Metabolite of the strain also plays a positive role in the process of relieving plant salt stress, and it is prompted that the strain possibly has the stability advantage in the aspect of environmental adaptability.

The invention discloses an enzymolysis gleditsia sinensis saponin-copper chelate as well as a preparation method and application thereof in prevention and treatment of citrus canker, and belongs to the technical field of crop prevention and treatment. The preparation method comprises the following steps: extracting gleditsia sinensis saponin, purifying the gleditsia sinensis saponin, carrying out enzymatic degradation on the gleditsia sinensis saponin, and then adding CuSO4. 5H2O to carry out chelation reaction to obtain the enzymolysis gleditsia sinensis saponin-copper chelate. The initial contact angle of an aqueous solution of the chelate on citrus leaves is small, the chelate can be rapidly wetted and spread on the leaves, the deposition effect is good, the chelate has excellent leaf adhesion performance and anti-scouring capacity, meanwhile, the enzymolysis gleditsia sinensis saponin-copper chelate has excellent inhibitory activity on xanthomonas carpesium, and the enzymolysis gleditsia sinensis saponin-copper chelate can be used for preparing the citrus leaf-copper chelate. The compound can be used as a green, environment-friendly, safe and efficient bactericide, and has excellent application potential in the aspect of citrus canker prevention and control.

The invention discloses a method for preparing PHA (polyhydroxyalkanoate) by using a complex microbial inoculant and application of the PHA. The method for preparing the PHA by using the complex microbial inoculant comprises the following steps: adding the complex microbial inoculant containing flavobacterium into a to-be-fermented liquid, and carrying out aerobic fermentation to obtain a PHA-rich fermented thallus; the liquid to be fermented contains organic acid feed liquid and optional water; the complex microbial inoculant disclosed by the invention comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by performing acid production fermentation on raw materials of enzymatic hydrolysate containing pentose and hexose. According to the present invention, the composite bacterial agent with the high organic acid utilization rate is prepared, and the multiple organic acid fermentation liquids obtained from lignocellulose can be effectively utilized by using the composite bacterial agent so as to perform PHA synthesis, such that the large-scale production of PHA and the resource utilization of lignocellulose are easily achieved.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

The invention relates to a bacterium and application thereof. The bacterium disclosed by the invention is (Xanthomonas sp.) PEG1; the preservation unit is CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC (China General Microbiological Culture Collection Center) The bacterium has significant positive application in prevention and killing of Eupatorium adenophorum seedlings. The fatality rate of the bacterium on ageratina adenophora seedlings is extremely high and reaches 96%, and the condition that all the seedlings are basically dead is presented. The invention can enrich the biological control agent of Eupatorium adenophorum and reduce the application of chemical herbicides, thereby reducing environmental pollution and protecting ecological balance.

The invention discloses a chlorohydrocarbon efficient synergistic degradation combined fungicide, a preparation method and application, and belongs to the technical field of microorganisms, the combined fungicide contains any one strain from rhodococcus or flavobacterium, and any one strain from bacillus, acinetobacter or comamonas. Through limitation of specific bacterium genus combination and artificial proportion optimization, the combined bacterium agent realizes a stable synergistic effect among different bacterium genus, the degradation efficiency is remarkably enhanced, and the 24-hour degradation rate of chlorinated hydrocarbon can be increased to 70% or above and is remarkably higher than that of a single bacterium system. Meanwhile, large-scale production can be achieved through the combined microbial agent, the overall cost is lower than that of a conventional scheme when the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, additional secondary treatment is not needed after repairing is completed, and economic benefits are remarkable. When the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, high-temperature treatment or chemical oxidation agents are not needed, and the combined microbial agent conforms to the development direction of current environmental protection laws and regulations and green restoration technologies.

The invention discloses a bagasse enzymatic hydrolysate detoxification process and application thereof in xanthan gum production, and relates to the technical field of bagasse enzymatic hydrolysate treatment and xanthan gum production. According to the bagasse enzymatic hydrolysate detoxification process, a quick lime detoxification method or a calcium phosphate detoxification method is adopted to treat bagasse enzymatic hydrolysate, and weak acid components, phenolic substances and furfural substances in the bagasse enzymatic hydrolysate are removed. The quick lime detoxification method comprises the following steps: adding quantitative calcium oxide solid into the bagasse enzymatic hydrolysate, continuously stirring, standing, primarily filtering to remove the solid, introducing carbon dioxide gas until the pH value of filtrate is less than or equal to 8, filtering again to remove the solid, and sterilizing the filtrate at high temperature. The detoxification process can efficiently remove inhibitors such as organic acids and / or inorganic acids, phenolic substances and furfural substances in the bagasse enzymatic hydrolysate, reduce inhibition on fermentation growth of xanthomonas campestris, significantly improve the yield of xanthan gum, provide a new application direction for the bagasse enzymatic hydrolysate, provide a new fermentation raw material for xanthan gum, and reduce the production cost. And the production cost is reduced.

The invention relates to the technical field of agricultural biology, in particular to a lyase compound preparation and application thereof in prevention and treatment of citrus canker, and the lyase compound preparation comprises lyase preparations LysXAC2, LysXAC4 and LysXAC5. An in-vitro bacteriostasis experiment shows that the lyase preparation forms an obvious splitting ring on an agar plate for the xanthomonas carpesium, and the growth of the xanthomonas carpesium can be effectively inhibited within 72 hours. Field application tests show that 25 L of the preparation is sprayed per mu after the preparation is diluted by 1000 times, the preparation starts to be applied in the early disease stage or the prevention stage and is applied once every 5 days, and the control effect can reach 83% or above after the preparation is continuously applied for 5 times. The preparation disclosed by the invention has the advantages of efficient sterilization, safety in use, environmental friendliness and the like, and a novel effective solution is provided for green prevention and control of citrus canker.

The invention relates to the technical field of molecular biology detection, and particularly discloses a characteristic nucleotide sequence, a specific primer pair, a kit and a detection method for detecting mango bacterial alternaria brassicae, three pairs of specific primers, namely 51-F / 51-R, 52-F / 52-R and 56-F / 56-R, are screened and designed from a conserved region of a pathogenic bacterium specific gene, and the specific nucleotide sequence, the specific primer pair, the kit and the detection method are used for detecting the mango bacterial alternaria brassicae. The lengths of amplification products are respectively 229 bp, 285 bp and 319 bp, and a conventional PCR (Polymerase Chain Reaction) and fluorescent quantitative PCR (Quantitative and Quantitative) detection method is established based on the primer pairs. The primer pair and the method disclosed by the invention have the advantages of strong specificity, high sensitivity and good stability, can be stably amplified from 34 target bacteria, and have no cross reaction on other related xanthomonas, other bacteria and fungi, and the fluorescent quantitative PCR detection limit can reach 1 pg / mu L; the invention provides reliable, rapid and multi-choice technical support for early diagnosis, disease monitoring and scientific prevention and treatment of mango bacterial black spot.

This invention discloses a sugarcane white streak disease resistance gene. ShPR10 The cloning and application of its promoters belong to the field of bioengineering technology. ShPR10 The nucleotide sequence of the gene is shown in SEQ ID NO: 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO: 2. This invention discovers... ShPR10 The gene can respond to Xanthomonas aureus infection stress, during Xanthomonas aureus infection. ShPR10 Gene expression levels increased significantly. Further investigation using a transgenic Arabidopsis system confirmed this. ShPR10 Genes can positively regulate plant disease resistance, that is, by overexpressing genes in plants. ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR This invention aims to improve plant resistance to pathogenic tomato species of *Pseudomonas syringae*. It provides a theoretical basis for elucidating the stress response mechanism of sugarcane to *Xanthomonas syringae* infection and also offers valuable genetic resources for disease-resistant sugarcane breeding.

The invention relates to bacillus paralicheniformis HMF14, and the preservation number of the bacillus paralicheniformis HMF14 is CGMCC (China General Microbiological Culture Collection Center) No.30480. The bacillus paralicheniformis HMF14 has the advantages that the preservation number is CGMCC No.30480; the bacillus paralicheniformis HMF14 disclosed by the invention can resist saline and alkaline, can antagonize fusarium oxysporum, hematoptera graminearum var. Graminearum and xanthomonas brassicae specialized wheat at the same time, also has a poisoning effect on pratylenchus agilis and paenchus calcarinus, and can be used for effectively preventing and treating wheat take-all and wheat black glume disease.

This invention discloses the construction and application of a xanthan gum cell factory with short fermentation cycle, high potency, and high resistance to oxidative stress. Using *Xanthomonas aeruginosa* XC1 as the starting strain, this invention employs a multi-step chromosome integration strategy to achieve the synergistic effect of three major modules: "enhancing quorum sensing signals, strengthening the rate-limiting synthesis step, and improving antioxidant performance." The resulting engineered strain EG-Y6 showed a 66-fold increase in intracellular DSF levels compared to the original strain XC1, significantly advancing the efficient xanthan gum synthesis period and shortening the fermentation cycle from 72 hours to 48 hours. The xanthan gum fermentation potency reached 26.9 g / L, a significant increase of 33.2% compared to the basic strain XC1. In the later stages of fermentation, the intracellular glutathione (GSH) content was 2.22 times higher than that of the basic strain XC1, while the reactive oxygen species (ROS) level decreased by 57%.

The invention relates to preparation of a multi-strain-ceramsite carrier compound and application of the multi-strain-ceramsite carrier compound in denitrification purification of aquaculture wastewater, and belongs to the field of aquaculture wastewater treatment. The compound is prepared from various strains such as aspergillus niger, pseudomonas fluorescens, bacillus subtillis, bacillus licheniformis, rhodopseudomonas palustris, pediococcus pentosaceus, nitrosomonas, paracoccus denitrificans and cellulomonas flavigena, and the strains are fixed in a ceramsite carrier by adopting a specific process. In the aquaculture sewage treatment process, the compound is added in a layered manner, so that efficient denitrification and decontamination of sewage are realized, and generation of odor is effectively inhibited. Practical application data show that the removal rates of total nitrogen, ammonia nitrogen and COD (Chemical Oxygen Demand) in the sewage respectively reach 88.2%, 90.2% and 81.2% after the sewage is treated by the compound. By means of multi-strain collaboration, process optimization and spatial distribution regulation and control, efficient static treatment of aquaculture sewage is achieved, and an economical and feasible solution is provided for rural environment pollution control.

Compositions and methods for orienting proteins to specific loci in the genome and uses thereof are disclosed. In one aspect, the disclosed methods allow for targeting a protein to a particular locus in a genome of an organism, including the step of providing a fusion protein comprising a DNA localization component and an effector molecule. Preferred embodiments of the present disclosure include, but are not limited to, the following fusion proteins: dSaCas9-Clo051, dCas9-Clo051, Xanthomonas sp. TALE-Clo051, and Ralstonia sp. TALE-Clo051.

The invention discloses an antibacterial peptide PXJ11 and application of the antibacterial peptide PXJ11. According to the present invention, an amino acid sequence represented by SEQ ID NO.1 is subjected to acetyl end-capping and amino end-capping modification to obtain the antibacterial peptide having inhibition effects on a variety of pathogenic bacteria, and the antibacterial peptide is named as antibacterial peptide PXJ11; the antibacterial peptide PXJ11 can inhibit pathogenic bacteria such as escherichia coli, staphylococcus aureus and xanthomonas citri, can also inhibit pathogenic fungi such as magnaporthe oryzae, and can be used for preventing and treating diseases / diseases caused by the pathogenic bacteria. The invention provides a novel biological control preparation for preventing and treating pathogenic bacteria such as xanthomonas citri and the like and diseases caused by the pathogenic bacteria, and the preparation is green and safe.