47 results about "Xanthomonas" patented technology

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention relates to the technical field of mineral processing, and particularly provides a combined reagent system for flotation and magnesium reduction of talc-containing copper-molybdenum ore and application of the combined reagent system. The combined reagent system comprises a flotation collecting agent (faint yellow to orange transparent liquid, 40%-50% of active ingredients and less than or equal to 3% of free alkali) with active ingredients in the formula 1 and a flotation depressing agent (light yellow powder, extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of 1000-1500000 Daltons and rich in hydroxyl, carboxyl and pyruvic acid groups) with a structural formula in the formula 2, and the flotation depressing agent comprises extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of xanthomonas and the weight-average molecular weight of xanthomonas and is rich in hydroxyl, carboxyl and pyruvic acid groups. Through the synergistic adsorption effect of the combined reagent, the flotation grade and the recovery rate of the copper sulfide molybdenum ore are remarkably improved under the condition that the pH is 8-11. The combined reagent system has the advantages of being high in flotation efficiency, high in selectivity, low in reagent cost, environmentally friendly, high in industrial applicability and the like.

The invention belongs to the technical field of genetic engineering, and particularly relates to a citrus canker pathogen mediated citrus leaf transient expression method which comprises the following steps: preparing a citrus canker pathogen infection solution which is xanthomonas citrus subspecies; infecting the citrus by using the citrus canker pathogen infection liquid, and culturing to obtain citrus infected by the citrus canker pathogen; preparing an agrobacterium infection solution containing a target gene; and inoculating the citrus infected by the citrus canker pathogen with an agrobacterium infection solution containing a target gene, and carrying out transient expression of the target gene. The transient expression method provided by the invention can realize transient and efficient expression of the target gene in leaves of different citrus varieties. The transient expression method disclosed by the invention can be used for researching gene functions, protein interaction, promoter activity analysis and the like; and the method can also be used for evaluating the citrus canker resistance gene function.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention belongs to the technical field of genetic engineering, and particularly relates to application of a negative regulation XC1141 gene in improving the yield of xanthan gum produced by xanthomonas campestris var. Campestris. According to the invention, an Xcc 8004 genome is subjected to knockout modification, and a gene XC1141 in the Xcc 8004 genome is deleted, so that an Xcc 8004 mutant strain (mutant strain Xcc 1141) with high yield of xanthan gum is obtained; the xanthan gum yield of the obtained Xcc 8004 mutant strain can reach 9.11 g / L, and the yield is increased by 65% compared with that of wild fungus Xcc 8004 (5.52 g / L).

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

The invention provides a carpet grass xanthomonas phage, a compound phage preparation and application of the carpet grass xanthomonas phage and the compound phage preparation. The carpet grass xanthomonas phage is carpet grass xanthomonas phage XAC17P1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241062. The compound bacteriophage preparation provided by the invention has higher sterilization efficiency and better application stability, belongs to a green and efficient plant disease biological prevention and control means, and can effectively prevent and treat citrus canker.

The invention belongs to the technical field of gene engineering, and particularly relates to a preparation method and application of an engineering strain NK-02 for xanthan gum production. The preparation method of the engineering strain for xanthan gum production comprises the following steps: taking xanthomonas campestris with a xanthan gum production function as a basic strain, and knocking out a gumF gene, a gumG gene and a gumL gene in the basic strain; inserting a synthetic biological element fimE-CI857; and introducing an overexpression plasmid to obtain the xanthan gum production engineering strain. According to the preparation method of the engineering strain for xanthan gum production, xanthan gum with different properties can be produced by controlling the fermentation temperature of the engineering strain, and an effective way is provided for providing a controllable xanthan gum production method.

The invention discloses a lyase preparation for preventing and treating rice bacterial blight and a preparation method and application thereof, and relates to the technical field of agricultural biology.The lyase preparation comprises one or more of lyase LysXO1 of xanthomonas phage GRNXOP1, lyase LysXO5 of xanthomonas phage GRNXOP5 and lyase LysXO6 of xanthomonas phage GRNXOP6; wherein the amino acid sequence of the LysXO1 is as shown in SEQ ID NO. 1; the amino acid sequence of the LysXO5 is as shown in SEQ ID NO. 2; and the amino acid sequence of the LysXO6 is as shown in SEQ ID NO. 3. The lyase preparation provided by the invention has the advantages of being efficient in sterilization, safe to use, environment-friendly and the like, and a novel effective solution is provided for green prevention and control of rice bacterial blight.

This invention discloses an R-type α-fluoroimine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from *Xanthomonas aeruginosa*. Luteolibacter luteus By using directed evolution to modify its active site, an imine reductase mutant with high activity, stereoselectivity, and high yield of 4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline was obtained. The mutation is at least one of S237T, I241L, or R248L. This invention achieves efficient synthesis of (R)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline from inexpensive (Z)-2,2,2-trifluoro-N-(4-methoxyphenyl)-1-phenylethylimine, which is beneficial for the industrial synthesis and application of (R)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline.

The present application discloses a microfluidic chip for detecting quarantine pathogens of raw grains. Each microfluidic chip of the present application includes at least 8 detection channels, and each detection channel includes at least 48 reaction zones, and the 48 reaction zones are used for detecting 11 kinds of quarantine pathogens of raw grains; the 11 kinds of quarantine pathogens of raw grains include **Diaporthe phaseolorum var. caulivora**, **Phialophora gregata**, **Fusarium virguliforme**, **Curtobacterium flaccumfaciens pv. flaccumfaciens**, **Tilletia indica**, **Tilletia walkeri**, **Wheat streak mosaic virus**, **Xanthomonas stewartii subsp. stewartii**, **Clavibacter michiganensis subsp. nebraskensis**, **Maize chlorotic mottle virus** and **Clavibacter michiganensis subsp. michiganensis**. The microfluidic chip of the present application can simultaneously detect 11 kinds of quarantine pathogens of raw grains for 8 samples to be tested, improves the detection efficiency, and has important significance for the inspection and quarantine work of quarantine pathogens of raw grains. **Note**: The scientific names of the pathogens are provided in Latin for accuracy in the technical field, and they can be adjusted according to specific requirements or common usage in the relevant industry. If you need to use more common or simplified names, please let me know and I can modify accordingly.

This invention belongs to the field of genetic engineering and biotechnology, specifically relating to a β-glucosidase PO3 for the conversion of rare ginsenosides, its preparation method, and its applications. This invention obtains β-glucosidase PO3 by PCR amplification using genomic DNA of *Xanthomonas pseudoepiplo* TY3-10 as a template. The results of the examples show that this enzyme can not only convert total ginsenosides into rare ginsenosides F2, C-O, C-Mx1, and C-Mc1, but also convert ginsenoside Rb1 into F2, ginsenoside Rb2 into C-O, ginsenoside Rb3 into C-Mx1, and ginsenoside Rc into C-Mc1; it also exhibits excellent catalytic performance and high expression efficiency, possessing extremely high application value and commercial prospects.

This invention discloses a strain of *Flavobacterium huinanense*, an inoculant, and its application in the control of potato scab, belonging to the field of green control technology for potato scab. This invention isolates a strain of *Flavobacterium huinanense* from the soil surface of potatoes. Flavobacterium anhuiense R10, which has a significant antagonistic effect on *Streptomyces bozzolani*, the pathogen of potato scab. *Flavobacterium huinanense* ( Flavobacterium anhuiense The fermentation broth of R10, when used for root irrigation of potatoes, can significantly reduce the incidence and severity index of potato scab.

The invention provides a carpet grass xanthomonas bacteriophage XAC18P1 capable of preventing and treating citrus canker and application thereof, and belongs to the technical field of biology, the bacteriophage is preserved in China Center for Type Culture Collection on May 24, 2024, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M 20241060. The bacteriophage XAC18P1 provided by the invention is wide in host range, the splitting rate is as high as 100%, and the bacteriophage XAC18P1 has relatively strong bactericidal ability and can be used for prevention and control and treatment of citrus canker, so that the incidence rate of citrus seedlings is reduced by 90% or above. In addition, the bacteriophage is high in fermentation rate and strong in stability, and has a good commercial prospect.

The invention relates to the technical field of new strains for degrading cellulose, in particular to a pseudoxanthomonas strain JC1303 and application thereof. Through whole genome sequencing and function annotation, it is systematically revealed for the first time that the strain carries a complete cellulose degrading enzyme system including incision beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and can be used for degrading cellulose. And specific gene resources are determined through generic genome analysis. The strain has the remarkable technical effects that the strain shows higher cellulase activity after being cultured for 5 days, and cellulose materials such as agricultural wastes and the like can be stably and efficiently degraded in a salt-containing environment by virtue of the unique genetic background and marine source characteristics of the strain.

The structural formula of the thymol-containing quaternary ammonium salt derivative is shown as (1), wherein R1 is 2-isopropyl-5-methylphenyl, ethyl or a benzene ring, R2 is 2-chloro-5-methylthiophene, 3-methyl-2-methoxycarbonyl furan, 4-methylnaphthalene and differently substituted benzyl, X-is Cl-and Br-, and n is equal to 3, 5, 7, 8 and 9. The preparation method of the drug-loaded antibacterial liposome comprises the step of jointly assembling hydrogenated soybean phosphatidylcholine, cholesterol hemisuccinic acid monoester, phosphatidyl ethanolamine and a target compound thymol-containing quaternary ammonium salt derivative into the drug-loaded antibacterial liposome. Part of the compounds have excellent antibacterial activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri and Xanthomonas syringae pv. Kiwifruit. Meanwhile, the compound also has excellent biological activity on six fungi such as fusarium oxysporum and TMV viruses. A field test on Xoo shows that the protection effect of the drug-loaded antibacterial liposome is improved by 1.94 times, the treatment effect is improved by 1.56 times, and the drug-loaded antibacterial liposome shows remarkable pH-triggered release characteristics and colloidal stability. (1)

The present invention relates to the application of polyether compounds as fungicides in controlling plant diseases caused by Xanthomonas, specifically, the polyether compounds are one or more of nigericin, lasalocid and nanchangmycin, which have a stronger inhibitory effect on Xanthomonas than the currently commonly used commercial drugs bismerthiazol and thiodiazole copper, and have good development prospects.

The invention discloses wide-temperature-range low-temperature beta-galactosidase OUC-JST derived from flavobacterium fusiformis as well as a coding gene and application of the wide-temperature-range low-temperature beta-galactosidase OUC-JST, and belongs to the technical field of functional enzymes. The beta-galactosidase OUC-JST has an amino acid sequence as shown in SEQ ID No.1, the optimum reaction temperature is 20 DEG C, the relative enzyme activity in a wide temperature range from 5 DEG C to 30 DEG C is kept at 50% or above, 20% of the enzyme activity is still kept at 0 DEG C, and the beta-galactosidase OUC-JST shows excellent wide-temperature-range low-temperature adaptability. The invention also provides a gene SEQ ID No.2 for coding the enzyme, a recombinant expression vector and recombinant engineering bacteria containing the gene, and a preparation method of the enzyme. The beta-galactosidase OUC-JST disclosed by the invention is suitable for hydrolyzing lactose at low temperature, and has a wide application prospect in the field of lactose-free dairy product production.

The invention relates to the technical field of image processing, in particular to an improved YOLOv8-based bacillus disease identification method, which comprises the following steps: acquiring a crop disease leaf image; an improved YOLOv8 model is constructed; wherein in the Backbone network, C2f is replaced by a C2f-CBAM module, and the C2f-CBAM module is embedded into a CBAM module in front of an output feature map of the C2f module; and in the check network, a standard convolution module is replaced by a GSConv module. The method solves the problems that a YOLOv8 model is limited in small target detection capability, blurred in scab edge and insufficient in local interference strong processing capability.

This invention discloses a metal ion-driven meliosteum peptide nanoassembly, its preparation method, and its applications, relating to the field of agricultural biotechnology. The meliosteum peptide nanoassembly is formed by the self-assembly of a hexahistidine-tagged meliosteum peptide with divalent metal ions through coordination. The average particle size of the meliosteum peptide nanoassembly is 20 nm–30 nm. The meliosteum peptide nanoassembly obtained by this invention through the self-assembly of a hexahistidine-tagged meliosteum peptide with zinc ions exhibits excellent antibacterial, anti-biofilm activity, stability, and biocompatibility, and can be used to control plant bacterial diseases caused by Xanthomonas and other fungi.

This invention discloses a novel salt-tolerant fungal strain, *Luteimonas sp.* R10, its prepared microbial agent, and its applications. The strain was isolated from the rhizosphere soil of cotton in saline-alkali land in Xinjiang. The microbial agent was obtained through fermentation. Treatment of wheat seeds with the *Luteimonas sp.* R10 microbial agent provided by this invention significantly improved wheat germination rate, budding rate, plant height, chlorophyll content, and phenylalanine hydrolase activity, increasing these by 26.67%, 21.28%, 41.34%, 98.73%, and 61.48%, respectively, compared to the control. When applied to cotton seed soaking, the cotton germination rate, plant height, and fresh weight increased by 200.00% compared to the control. 、 The concentrations of 31.50% and 9.37% indicate that the microbial inoculant prepared from the salt-tolerant new bacterium Luteimonas sp. R10 provided by this invention has a significant promoting effect on plant growth and early maturity, can increase plant disease resistance, and has good application prospects as a bio-fertilizer.

The invention discloses xanthomonas for promoting plant growth and resisting salt stress and application of the xanthomonas. The invention provides Xanthomonas sontii, the strain number of the Xanthomonas sontii is JT6-1, and the registration number of the Xanthomonas sontii in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.34844. The Xanthomonas sontii JT6-1 strain provided by the invention has salt-tolerant and drought-resistant capabilities, and in combination with pot experiment results, the JT6-1 strain shows good growth-promoting characteristics under salt stress, so that the application potential of the JT6-1 strain in improvement of plant stress resistance is further proved, the JT6-1 strain not only can be used as the JT6-1 strain, but also can be used as the JT6-1 strain, the JT6-1 strain and the JT6-1 strain, and the JT6-1 strain and the JT6-1 strain can be used for improving plant stress resistance. Metabolite of the strain also plays a positive role in the process of relieving plant salt stress, and it is prompted that the strain possibly has the stability advantage in the aspect of environmental adaptability.

The present invention discloses a benzylpiperidine-containing formononetin derivative, a preparation method, and an application thereof, belonging to the technical field of pesticide synthesis. The present invention introduces a benzylpiperidine group with excellent activity into the structure of formononetin to synthesize a series of formononetin derivatives containing benzylpiperidine. Testing of the inhibitory activity of the synthesized formononetin derivatives containing benzylpiperidine against plant pathogenic bacteria reveals that the synthesized formononetin derivatives containing benzylpiperidine can effectively inhibit plant pathogenic bacteria, particularly Xanthomonas.

Provided herein are polypeptides, compositions comprising the polypeptides and methods for genome editing and gene regulation (e.g., activation and / or repression) using the polypeptides or the compositions comprising the polypeptides, such as, DNA binding domains derived from the genus of Ralstonia. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a TALE protein, such as, a Xanthomonas TALE protein. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a DNA binding protein derived from bacteria of the genus Ralstonia.

The present invention belongs to the field of medicinal chemistry, and discloses the use of a class of diaminopyrimidine compounds in preventing and treating plant diseases caused by rice bacterial blight pathogen Xanthomonas oryzae ACCC 11602, citrus canker pathogen Xanthomonas axonopodis pv.Citri and potato black shank pathogen Pectobacterium atroseptica ACCC 19901. The structure is as follows: The diaminopyrimidine compounds have the characteristics of simple structure, easy synthesis, low price, wide application, etc., and through activity determination, it is found that the diaminopyrimidine compounds have clear structure-activity relationship and wide fungicide spectrum, and are worth further research and development.

To control plant diseases caused by Xanthomonas spp., a novel bacteriophage exhibiting bacteriolysis activity specifically on Xanthomonas spp. was isolated. Thus, developed and provided is a plant disease control composition containing the bacteriophage as an active ingredient. Provided are a bacteriolytic agent containing a bacteriophage that has a novel genomic DNA sequence and exhibits bacteriolysis activity specifically on Xanthomonas spp., and a plant disease control composition containing the same as an active ingredient.

The invention discloses an enzymolysis gleditsia sinensis saponin-copper chelate as well as a preparation method and application thereof in prevention and treatment of citrus canker, and belongs to the technical field of crop prevention and treatment. The preparation method comprises the following steps: extracting gleditsia sinensis saponin, purifying the gleditsia sinensis saponin, carrying out enzymatic degradation on the gleditsia sinensis saponin, and then adding CuSO4. 5H2O to carry out chelation reaction to obtain the enzymolysis gleditsia sinensis saponin-copper chelate. The initial contact angle of an aqueous solution of the chelate on citrus leaves is small, the chelate can be rapidly wetted and spread on the leaves, the deposition effect is good, the chelate has excellent leaf adhesion performance and anti-scouring capacity, meanwhile, the enzymolysis gleditsia sinensis saponin-copper chelate has excellent inhibitory activity on xanthomonas carpesium, and the enzymolysis gleditsia sinensis saponin-copper chelate can be used for preparing the citrus leaf-copper chelate. The compound can be used as a green, environment-friendly, safe and efficient bactericide, and has excellent application potential in the aspect of citrus canker prevention and control.

The invention discloses a method for preparing PHA (polyhydroxyalkanoate) by using a complex microbial inoculant and application of the PHA. The method for preparing the PHA by using the complex microbial inoculant comprises the following steps: adding the complex microbial inoculant containing flavobacterium into a to-be-fermented liquid, and carrying out aerobic fermentation to obtain a PHA-rich fermented thallus; the liquid to be fermented contains organic acid feed liquid and optional water; the complex microbial inoculant disclosed by the invention comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by performing acid production fermentation on raw materials of enzymatic hydrolysate containing pentose and hexose. According to the present invention, the composite bacterial agent with the high organic acid utilization rate is prepared, and the multiple organic acid fermentation liquids obtained from lignocellulose can be effectively utilized by using the composite bacterial agent so as to perform PHA synthesis, such that the large-scale production of PHA and the resource utilization of lignocellulose are easily achieved.

The invention discloses citrobacter sp. And application thereof in soybean planting, and belongs to the technical field of biological agriculture. The citrobacter sp. NJC42 is preserved in the China Center for Type Culture Collection, the preservation number of the citrobacter sp. NJC42 is CCTCC No.M 20242057, and the preservation date of the citrobacter sp. NJC42 is September 24, 2024. The citrobacter sp. NJC42 disclosed by the invention not only can inhibit the growth of Xanthomonas axonophora pv.glycines, is used for preparing a preparation for preventing soybean bacterial leaf spot, but also can be used for promoting the yield increase of soybeans, and is used for preparing a preparation for promoting the yield increase of the soybeans, so that the citrobacter sp. NJC42 disclosed by the invention can be used for inhibiting the growth of the Xanthomonas axonophora pv.glycines, and the citrobacter sp. NJC42 disclosed by the invention can be used for preparing a preparation for promoting the yield increase of the soybeans.

The invention discloses a high pyruvic acid xanthan gum production strain and application, and relates to the technical field of biology, the xanthan gum production strain is Xanthomonas campestris H1, and is preserved in the Guangdong Microbial Culture Collection Center on February 20, 2025, the preservation number is GDMCC NO: 65930, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The strain or fermentation liquor thereof can be used for preparing pyruvic acid. According to the method, the Xanthomonas campestris H1 strain is successfully bred through combination of normal-temperature and normal-pressure plasma (ARTP) mutagenesis and a high-throughput screening technology, the strain shows high pyruvic acid content in the fermentation process, the content of pyruvic acid groups in xanthan gum molecules can be effectively increased, and therefore the requirement of the market for high-performance xanthan gum is met.