171 results about "Xanthomonas" patented technology

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and / or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

The invention discloses burkholderia cepacia SD7 and a culturing method and application thereof. The bacterial strain is preserved in China General Microbiological Culture Collection Center on October 24th, 2001, and the preservation number is CGMCC No.5384. Through research, the bacterial strain has strong inhibiting effect on rhizoctonia solani, collectotrichum musae, pyricularia grisea, gibberella zeae, fusarium oxysporum f. sp. cubense, peronophythora litchi, ustiago scitamimea syd, fulvia fulva, xanthomonas oryzaepv.oryzae, xanthomonas oryzae pv. Oryzicola, and the like, is broad-spectrum antagonistic bacteria, can be used for preventing and treating a plurality of plant diseases, particularly has better preventing and treating effect for banana fruit anthracnose and rice sheath blight, has the advantages of harmlessness to person and animals, environmental protection and safety, good environmental compatibility, and difficulty in becoming resistant, is good for business development, and has broad application prospect.

The invention relates to organisms and compositions comprising one or more stem cells or one or more embryos, wherein the one or more stem cells or one or more embryos comprise one or more of the following mutations: (i) a deletion mutation; (ii) a knockout mutation; and / or (iii) an addition of a heterologous nucleic acid sequence; wherein the one or more mutations of (i), (ii), and / or (iii) are site-specific mutations caused by a Xanthomonas TAL nuclease (XTN). The invention also relates to method of mutating an embryo, iPS cell, stem cell, or more particularly a spermatogonial stem cell by exposing the nucleic acid sequence contained within such embryos or cell with a Xanthomonas TAL nuclease.

The invention discloses a compost inoculant and applications thereof. The active ingredients of the composting inoculant provided by the invention include clostridium thermocellum, geobacillus stearothermophilus, Taiwan false xanthomonas, brevibacillus agri and bacillus licheniformis in a CFU (colony-forming unit) ratio of (3-5):(1-2):(1-2):(1-2):(1-2). Strains in the inoculant provided by the invention can grow in a symbiosis state well, and under the conditions of microaerobic co-culturing, lignocelluloses can be regularly proliferated and effectively decomposed in proportion. The inoculant is simple in manufacturing process due to co-culturing, stable in decomposition function, and long in quality ensured period. Experiments show that when the inoculant is adopted for fermenting composts, large gas feeding is not required, the energy is saved, and the nitrogen loss is reduced; composts can be thoroughly decomposed in advance, thereby improving the quality of fertilizers.

The invention discloses a new Rahnella aquatilis strain HX2 (CGMCC No.1896), which is characterized by the following: manufacturing antagonistic material and IAA; inhibiting partial pathogenic bacteria (Agrobacterium, Xanthomonas, Clavibacter, Pseudomonas and Bacillus) and partial pathogenic fungus (Fusarium, Alternaria and Botrytis); detecting root cancer based on green house sunflower as indicating plant.

The invention discloses a xanthomonas Xanthomonassp. S-96#, preparation method thereof and a method thereof for producing temperature resistant xanthan gum polysaccharide, including seed selection of temperature resistant high viscosity strain, expanding culture of seed, fermentation and extraction processing steps. The strain of the invention has good temperature resistance, the produced xanthan gum polysaccharide product has stable quality, the viscosity of the product after being heated is obviously higher than similar product, application indexes are excellent, and the original production process of similar products is improved, production process is safe, thus being capable of substituting the existing xanthan gum product and being widely applied to the petroleum development industries requiring high viscosity under high temperature condition.

The invention discloses a method for improving the output of crude oil by utilizing a synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria. The method comprises the steps: injecting diluent of a compound microorganism bacterium agent prepared from pseudomonas fermentation liquor with a phosphate-solubilizing function, azotobacter fermentation liquor and combined bacteria liquid with a petroleum hydrocarbon degrading function into an oil pool via a water injection well or injecting through a production well, and extracting oil to obtain the crude oil. The compound microorganism bacterium agent is prepared from the pseudomonas fermentation liquor, the azotobacter fermentation liquor and the combined bacteria liquid with the petroleum hydrocarbon degrading function according to a ratio of 1 to 1.2 to 1.6-8; the combined bacteria liquid with the petroleum hydrocarbon degrading function contains bacillus, acinetobacter and xanthomonas; and the three bacteria are independently cultivated into single bacteria liquid of 108-1010cfu / ml, and then mixed iso-volumetrically, so that the combined bacteria liquid with the petroleum hydrocarbon degrading function is obtained. According to the invention, the method is simple in construction, short in time, low in cost, large in yield rate and high in oil extraction productivity.

The invention relates to the technical field of plant gene engineering and provides an inducible promoter for pathogenic bacteria of rice. The promoter can be activated by rice bacterial leaf blight bacterium xanthomonas PXO99 and PXO61, a rice baeterial leaf streak bacterium RS105 and a corn banded sclerotial blight bacterium YWK-196, and can also be activated by abscisic acid, gibberellic acid, salicylic acid and sodium chloride. Truncation of the promoter verifies that two sections from -513bp to -411bp and from -411bp to -309bp are critical areas induced by the pathogenic bacteria. Various plant expression vectors can be constructed with the adoption of the promoter, and the promoter can be used for improving plant quality and other beneficial production traits through a plant gene engineering technology.

The invention discloses a Xanthomonas amylose culture medium, a method for preparing the culture medium and the application of the culture medium in the production of Xanthomonas amylose; the raw materials used for preparing the culture medium comprises starch waste water, amylase, active medical stone granule and yeast extract; the preparation method comprises the steps of wastewater disposal, pH value control, zymohydrolysis and saccharification, as well as depigmentation and adding nutrientsubstance; the method has the advantages that the starch waste water is transformed into the culture medium for producing the Xanthomonas amylase, thus greatly reducing the production cost and realizing the technological innovation of the industry of the Xanthomonas amylose; meanwhile, the method can turn harm into good and change waste into valuable, so as to reduce the pollution of organic wastewater of starch for the environment and develop circular economy in 'second panning' of the starch waste water.

The invention discloses pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops. The pseudomonas fluorescens SC3 is collected in the Guangdong Microbial Culture Collection Center, with the collection number GDMCC NO. 60367, at the date of April, 30th, 2018. The The pseudomonas fluorescens SC3 has remarkable suppression effect on various bacterial diseases, especially on bacterial soft rot of crops, ralstonia solanacearum and xanthomonas, reduces incidence rate of bacterial soft rot of crops, and has good prevention and control effect in potted experiments. Besides, the pseudomonas fluorescens SC3, which is avirulent, can be used for development of biopesticide for treating crop bacterial diseases and provides a new thought and idea about biological prevention and control of bacterial soft rot of crops.

The invention relates to a notoginseng soil treatment agent and a use method thereof. The notoginseng soil treatment agent contains 50% of carbendazol wettable powder, 95% of hymexazol wettable powder, 25% of metalaxyl, propamocarb wettable powder and 72% of agricultural use streptomycin wettable powder. The soil treatment agent has good effects to prevent major fungus diseases caused by ascomycetes, deuteromycetes, oomycetes in fungus and diseases caused by pseudomonas and xanthomonas bacteria; soil treatment is carried out on soil which is used for continuously planting for four years with the soil treatment agent 20 days before notoginseng transplanting and a notoginseng seed dressing. Rate of emergence of a field trial is 87.22% which is 63.63% higher than that of a comparison without using the soil treatment agent of 23.59 percent. The soil treatment agent has low toxicity to human and livestock and less vestigital, and not only can be used for preventing and treating bad rot, stump rot, bud-rot and diseases of the notoginseng, but also can be used for treating the soil of various crops of other traditional Chinese medical materials and vegetables.

In order to provide a method for culturing an immunopotentiator-containing organism having the experience of being eaten, inexpensively without requiring usage of a component derived from an animal, Acetobacter, Gluconobacter, Xanthomonas, Zymomonas or Enterobacter which is an edible gram-negative bacterium having an immunopotentiation function is cultured using a culture solution composed mainly of wheat or bean curd refuse. Thereby, Acetobacter can be obtained inexpensively and safely, and also a low molecular weight lipopolysaccharide which is the immunopotentiator can be obtained inexpensively and safely. Furthermore, no impurity derived from animal components is mixed.

A composition for treating Xanthomonas spp. infections in agriculture crops wherein the treatment composition is an admixture of T. harzianum, Y. schidigera root extract, Y. schidigera leaf extract, and X. spp. extract. The composition is also efficacious as a pre-infection agent for enhancing the resistance of agriculture crops to such infections. The composition is preferably applied by spraying.

The invention discloses a treatment method for removing lead in electroplating wastewater. The wastewater is sequentially treated by a collecting well, a coarse screen, a primary sedimentation tank, a pH value adjusting tank, a biological fermentation-sedimentation reaction purifying system, an aerobic biofilter, a secondary sedimentation tank and a water purifying tank. In the method, the biological fermentation characteristics of vegetable xanthomonas are utilized creatively; in the presence of D-glucose, a lactonization reaction is performed in the vegetable xanthomonas, and finally L-ascorbic acid (vitamin C) is produced by fermentation; and based on the characteristic that the L-ascorbic acid can be combined with lead ions to generate water-insoluble and nontoxic salt, the lead ions in the electroplating wastewater can be removed in a sedimentation process. Meanwhile, due to the utilization of the L-ascorbic acid produced by adopting D-glucose in the biological fermentation reaction, the cost is far lower than that of directly adding L-ascorbic acid into the wastewater, and the material cost in wastewater treatment can be remarkably reduced.

The invention relates to the technical field of microorganisms, in particular to a strain of pseudomonas having significant antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of the pseudomonas. The strain is isolated from rice rhizosphere soil of Fengxian District, Shanghai; the pseudomonas provided by the invention was deposited in the China Center for Type Culture Collection on May 7, 2018, the preservation name is Pseudomonas mosselii 923, and the preservation number is CCTCC No:M 2018252. The pseudomonas provided by the invention has a significant inhibitory effect on Xanthomonas oryzae, has relatively strong inhibitory capacity against other plant pathogenic bacteria and magnaporthe oryzae of Xanthomonas, and has biological control application value.

A composition for treating Xanthomonas spp. infections in agriculture crops wherein the treatment composition is an admixture of T. harzianum, Y. schidigera root extract, Y. schidigera leaf extract, and X. spp. extract. The composition is also efficacious as a pre-infection agent for enhancing the resistance of agriculture crops to such infections. The composition is preferably applied by spraying.

The present invention discloses applications of a Xanthomonas pathopoiesis-associated gene hpaM in prevention and control of plant Xanthomonas diseases, wherein the number of the gene is XC_2847, the gene encodes a cell membrane protein, and the plant Xanthomonas diseases comprises one or a plurality of diseases selected from cruciferae black rot caused by cruciferae black rot bacteria, rice bacterial blight caused by rice bacterial blight bacterial, and rice bacterial leaf streak caused by rice bacterial leaf streak bacteria.

The invention relates to a production process of instant noodles, in particular to a production process of sugar-free non-fired instant noodles. The production process of the sugar-free non-fried instant noodles provided by the invention is characterized by comprising the following steps of: preprocessing raw materials: preprocessing wheat bran, bean dreg and corn; kneading dough: weighing 1-300 parts by weight of plant noodle material, 0.001-0.05 part by weight of xanthomonas polysaccharide corn flour or 2-6 parts by weight of xanthan gum and 1-300 parts by weight of water and stirring to be uniform to make a flour dough, wherein the plant noodle material is one or more than one of wheat bran noodle material, bean dreg noodle material and corn noodle material; cutting into blocks; curing; and packaging. The sugar-free non-fired instant noodles produced by adopting the process contains abundant vitamins, crude cellulose, protein and minerals, has comprehensive nutrient components and are easy to be absorbed by human body; and the processability is enhanced when the process is adopted to produce the sugar-free instant noodles.

The invention discloses a phage water aqua preservative, a preparation method and an application thereof and belongs to the technical field of microbial antisepsis. The technical scheme is characterized in that the phage water aqua preservative comprises the following components by weight: 1-3 parts of sodium dehydroacetate, 7-9 parts of preservative and the balance of water aqua. The phage wateraqua preservative is applicable to strains such as a vibrio parahaemolyticus phage, an escherichia coli phage, a salmonella phage, a ralstonia solanacearum phage, a staphylococcus aureus phage, an aeromonas hydrophila phage, the ralstonia solanacearum phage, an axonopus scoparius xanthomonas and an agrobacterium tumefaciens. The preservative disclosed by the invention has the advantages of preventing bacterial growth, being non-toxic to the phages and improving the stability of the phages so that the phages are difficult to deteriorate and the stability of the titer is maintained. In a practical case, the preservative can play a good role in the prevention and control of animal and plant diseases, and is especially applicable to the industrial large-scale production of phage preparations and the process of user storage and field application.

The invention provides bacillus for antagonizing fusarium wilt and promoting growth and an application of the bacillus. The bacterial strain is bacillus simplex FJAT-47158, and the chemical name of the bacterial strain is Bacillus simplex FJAT-47158. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacterial strainis CGMCC NO. 15946. The bacillus disclosed by the invention not only can inhibit phytopathogens such as fusarium oxysporum, xanthomonas carpetalis and the like, but also can improve the activity of plant seeds and promote the growth of the seeds, can be used for preventing and treating fusarium wilt, and is an environment-friendly, economical and effective biocontrol bacterium for preventing andtreating diseases.

The present invention aims at providing a vaccine composition capable of being administered to an intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, or rectal mucous membrane, that is safe, useful as a prophylactic or therapeutic agent for infectious diseases or cancers, and capable of effectively inducing the systemic immune response and mucosal immune response. The present invention provides a mucosal vaccine composition to be administered to at least one mucous membrane selected from the group consisting of a human or animal intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, and rectal mucous membrane, the mucosal vaccine composition containing: at least one antigen; and as an adjuvant, a lipopolysaccharide derived from at least one gram-negative bacterium selected from the group consisting of Serratia, Leclercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter, or a salt thereof, wherein a mass ratio between the adjuvant and the antigen (total mass of the adjuvant / total mass of the antigen) is 0.002 to 500.

The invention provides a Xanthomonas oryzea pv.oryzae resistance related gene of wild rice, protein and uses, the multiple transcription polynucleotide sequences produced by RIX-4 gene alternative splicing and the corresponding open reading frame sequences, wherein the sequences possesses polynucleotide sequences represented by SEQ No.1-10, these cDNA fragments are closely related to the Xanthomonas oryzea pv.oryzae. The invention also provides the polynucleotide recombinant vector containing RIX-4 encoding genes and the gene engineering host cell, the method for cloning the RIX-4 genes, and the chromosome orientation of the RIX-4 genes.

The invention relates to the field of microorganisms, and particularly provides a microbial symbiotic culture inoculant and a preparation method and application thereof. The microbial symbiotic culture inoculant provided by the invention specifically comprises pseudomonas, bacillus subtilis, candida and xanthomonas. The inoculant is good in environmental adaptability and high in toxic pollution resistance, can be well applied to various petroleum pollution environments, achieves stable balance among strains through symbiotic culture, and can be prepared through large-scale fermentation. The target strains are co-cultured, a stable symbiotic system is formed by utilizing the interaction among the strains, the preparation method is simple, and the prepared microbial symbiotic culture inoculant is balanced and stable among the strains and high in environmental adaptability. The inoculant can treat petroleum sewage or petroleum sludge at a time, the treated petroleum sewage or petroleum sludge reaches the direct discharge standard, and the treatment cost is greatly reduced.

Applicants have successfully generated heritable phenotypes in plants making them resistant to bacterial blight. TAL effector binding elements, (EBEs) of bacterial pathogen disease susceptibility genes are modified to prevent induction of expression associated with disease states caused by the bacterial pathogens. Surprisingly, Applicants have found that modifications may be made in the EBEs of these genes which prevent bacterial pathogen induction, but still allow for normal plant development and seed production. Nucleic acid sequences for generating such plants, amino acid sequences, cells, vectors and expression constructs are included as well as resistant plants, seeds and lines.

The invention discloses a phathopoiesis related gene (cytosine deaminase gene) from Xanthomonas causing cabbage black rot. The cytosine deaminase gene provided by the invention contains one of the following nucleotide sequence: 1) DNA sequence shown in SEQ ID No.1, 2) DNA sequence containing over 80% homology with DNA sequence shown in SEQ ID No. 1. The invention also involves the application of the genes in plant disease prevention and cure, as well as the application for drug target in plant disease prevention and cure.

The invention relates to heterocyclic substituted 1, 3, 4-oxadiazole hydrazide compounds and a preparation method and application thereof. The compound has a structure shown as a general formula (I). The compound has a relatively good inhibition effect on pathogenic fungi and bacteria; and the compound has good inhibition effects onfusarium graminearum, potato late blight bacteria, blueberry root rot bacteria, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, botryosphaeria dothidea, rhizoctonia solani, xanthomonas oryzae pv., ralstonia solannacearum, Xanthomonas citri, kiwi canker bacteria, Xanthomonas oryzae cucumber, Xanthomonas oryza konjak, Botryosphaeria dothidea, tomato canker pathogens, apple canker pathogens and the like.

The invention discloses a rice XIOsPR10 gene and use thereof, which relate to the field of plant genetic engineering. The invention also provides a rice XIOsPR10 gene sequence, a gene encoding protein sequence, use of the gene in RNA enzyme digestion degradation, a method for constructing an over expression vector of the gene, a method for rice genetic transformation and the use of the gene in over expression in transgenic rice. After-infection expression up-regulating protein PR10 is obtained by analyzing rice leave expressing differential proteins before and after the infection with xanthomonas oryzicola fangetal bacteria. The product of the prokaryotic expression of the rice XIOsPR10 gene has RNase activity and can be used in RNA enzyme digestion degradation; by the genetic transformation of rice, transgenic rice plants with XIOsPR10 gene over expression are obtained and the xanthomonas oryzicola fangetal resistance of rice is improved, so the rice XIOsPR10 gene can be used in the prevention and control of xanthomonas oryzicola fangetal.

The invention discloses a thick broad-bean sauce chilli billet low-salt fermentation method based on microbial augmentation and original pool pouring. The thick broad-bean sauce chilli billet low-salt fermentation method based on microbial augmentation and original pool pouring comprises following steps: stem end of fresh chilli is removed, the fresh chilli is subjected to washing, draining, crushing, and salting with table salt for 3 to 10 days; after salting, an obtained chilli billet is inoculated with saccharomyces cerevisiae, bacillus subtilis, and lactobacillus plantarum for 10 to 20 days of fermentation, and then is inoculated with xanthomonas for 10 to 20 days of fermentation; and at last, fermentation is carried out for another 10 to 20 days with original pool pouring carried out for one time every 3 to 5 days so as to realize rapid fermentation of the chilli billet. The thick broad-bean sauce chilli billet low-salt fermentation method is capable of realizing low salt salting of chilli billet, realizing full utilization of chilli billet high salt leachate, reducing salt concentration of the leachate via microbial augmentation fermentation, realizing desalting of chilli billet via orginal pool pouring, and avoiding unstable fermentation caused by microorganisms introduced by chilli via step by step augmentation of chilli billet microbial fermentation process.

The invention discloses a bird rape xanthomonas essential gene and coded protein and application thereof. The essential gene has one of the following nucleotide sequences: (1) a DNA (Deoxyribonucleic Acid) sequence of SEQ ID No.1 in a sequence table, and (2) polynucleotide for encoding the SEQ ID No.2 of an amino acid sequence in the sequence table. The protein coded by the essential gene has an amino acid sequence of SEQ ID No.2 in the sequence table. The essential gene or the coded gene thereof can be taken as a target for screening substance resisting bird rape xanthomonas. The essential gene and the coded protein thereof are essential for the growth of bird rape xanthomonas, so that the essential gene and the coded protein thereof can be taken as ideal and specific pesticide acting targets of bird rape xanthomonas. The crucifer black rot caused by bird rape xanthomonas is a severe hazard to the production of a large quantity of vegetables in the world, so that the research, the development and the application of the protein and the gene play a unique role in controlling the block rot.