5results about How to "Increased transcript levels" patented technology

The application provides a promoter capable of improving transcription level of an adenylosuccinate lyase gene and application, the promoter is based on the sequence shown in SEQ ID NO: 1 and is subjected to mutation, and five promoters capable of strengthening transcription level of the adenylosuccinate lyase gene and capable of improving expression of an alpha-amylase amyE gene from Bacillus subtilis subsp. subtilis str. 168 in coryneform bacteria are obtained.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.

The application discloses lactobacillus plantarum WH021 and application thereof in prevention and / or alleviation of neuroinflammation, and belongs to the technical field of microorganisms. The lactobacillus plantarum WH021 is obtained by separation and purification from healthy infant feces, is a probiotic with the effect of alleviating neuroinflammation, and has good application potential in development of food, health products and drugs for preventing brain inflammation related diseases of middle-aged and old people caused by chronic inflammation of the body.

This invention provides extracellular vesicle miRNAs from milk for detecting heat stress in dairy cows and their applications, belonging to the field of dairy cow health technology. This invention, through the isolation, extraction, and miRNA sequencing of extracellular vesicles derived from dairy cow milk, discovered specific miRNA molecules closely related to heat stress. Using the miRNA unconservative_3_49980 from these extracellular vesicles as a biomarker, this invention provides a simple, non-invasive, and stress-free method for detecting heat stress in dairy cows with high sensitivity. This invention also designs specific primer pairs for the miRNA, enabling the construction of a kit for early, non-invasive assessment of heat stress in dairy cows.

The application of the OsWRKY7 gene in improving the cold tolerance of rice belongs to the field of biotechnology, and discloses application of a rice transcription factor OsWRKY7 in improving the cold tolerance of rice; the genomic DNA sequence of the rice transcription factor OsWRKY7 is shown as SEQ ID NO. 1. By means of CRISPR gene targeting editing technology, the OsWRKY7 gene is knocked out or its expression is down-regulated, and a rice mutant with a function loss or down-regulated expression of the gene is obtained, which can significantly improve the cold stress tolerance. Therefore, the OsWRKY7 has a good application prospect in enhancing the cold tolerance of rice and cultivating cold-tolerant rice varieties.