14results about How to "Increased transcript levels" patented technology

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The invention discloses plant lactobacillus WH021 and application thereof in preventing and / or relieving neuroinflammation, and belongs to the technical field of microorganisms. The plant lactobacillus WH021 is separated and purified from excrement of healthy babies, is a probiotic with the function of relieving neuroinflammation, and has good application potential in development of food, health care products and drugs for preventing brain inflammation related diseases caused by chronic inflammation of bodies of middle-aged and elderly people.

The invention provides a recombinant engineering bacterium for preparing recombinant albumin based on a transcription factor and application, and relates to the technical field of gene engineering. According to the present invention, while the recombinant albumin is expressed, the SUS1 gene and the SUA7 gene are simultaneously overexpressed and integrated into the strain, and / or one or more genes of YHP1 and HDA1 are subjected to targeted knockout by using the CRISPR-Cas9 gene editing technology so as to improve the expression quantity of the recombinant albumin; according to the recombinant engineering bacterium, the yield of recombinant albumin can be remarkably increased, and the recombinant engineering bacterium has a great application value.

The invention relates to a codon optimization method for improving the protein expression quantity of a target gene in a plant, and belongs to the technical field of gene engineering. The invention provides a codon optimization method for improving the protein expression quantity of a target gene in a plant, which comprises the step of replacing the third basic group of a codon of a target protein coding sequence with C or G to obtain an optimized target protein coding sequence. The method has effectiveness and universality in plants, and research shows that after codon optimization of the method, the protein expression quantity of target genes in a plant protein transient expression system and a transgenic plant is increased by several times to dozens of times. According to the production application of the functional protein taking the plant as the bioreactor, the yield of the target protein can be increased, and the protein purification cost can be reduced; according to the application of the method in preparation of the transgenic plant, the expression quantity of the target gene can be increased, so that the target character of the transgenic plant is enhanced, and the screening cost of the high-expression transgenic plant is reduced.

The invention discovers that N-Acetylneuraminic acid pyruvate lyase (N-Acetylneuraminic acid pyruvate lyase, NPL) is highly related to the neogenesis and development of vascular or lymphatic endothelial cells for the first time. Research results show that up-regulation of expression of N-acetylneuraminic acid pyruvate lyase can promote cavitalization of blood vessels or lymphatic vessels and relieve lumen stenosis; by down-regulating the expression of N-acetylneuraminic acid pyruvate lyase or inhibiting the activity of N-acetylneuraminic acid pyruvate lyase, cavitalization of blood vessels or lymphatic vessels can be inhibited, so that the blood vessels are closed or even deleted. Therefore, the N-acetylneuraminic acid pyruvate lyase can be used as a treatment target of the lumen stenosis disease and the hyperplasia of the blood vessel to guide the development of related medicines.

The application provides a promoter capable of improving transcription level of an adenylosuccinate lyase gene and application, the promoter is based on the sequence shown in SEQ ID NO: 1 and is subjected to mutation, and five promoters capable of strengthening transcription level of the adenylosuccinate lyase gene and capable of improving expression of an alpha-amylase amyE gene from Bacillus subtilis subsp. subtilis str. 168 in coryneform bacteria are obtained.

The invention relates to the technical field of bioengineering, in particular to anti-nitrite related miRNA, a precursor of the miRNA, a coding gene of the precursor and application of the coding gene. The nucleotide sequence of the miRNA related to nitrite resistance is as shown in SEQ ID NO. 1, and the precursor sequence of the miRNA related to nitrite resistance is as shown in SEQ ID NO. 2. Experiments prove that miR-151 participates in the physiological process of responding to nitrite stress under acute nitrite stress, and overexpression of miR-151 can promote urea generation, assist detoxification metabolism of ammonia poisoning and relieve cell damage caused by acute nitrite. Starting from the internal regulation of responding to nitrite stress, the invention verifies that miR-151 plays an important role in resisting nitrite stress, and provides a scientific basis for the breeding of nitrite-resistant excellent varieties and the optimization of culture environment management.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.

The present invention provides the application of AeMYB30 and / or AeUF3GaT1 in improving the fruit setting rate of golden sunflower, belonging to the field of plant cultivation and genetic engineering. In the present invention, the hyperoside improves the plant fruit setting rate through the mediation of the complex MYB30-UF3GaT1, and the plant fruit setting rate can be simply, quickly and efficiently improved by spraying the hyperoside preparation on the plant. Spraying the hyperoside preparation of the present invention can significantly improve the fruit setting rate and thousand-grain weight of understory economic plants. Taking golden sunflower as an example, the fruit setting rate can reach up to 87.4% and the thousand-grain weight can reach up to 80.3g.

The application discloses lactobacillus plantarum WH021 and application thereof in prevention and / or alleviation of neuroinflammation, and belongs to the technical field of microorganisms. The lactobacillus plantarum WH021 is obtained by separation and purification from healthy infant feces, is a probiotic with the effect of alleviating neuroinflammation, and has good application potential in development of food, health products and drugs for preventing brain inflammation related diseases of middle-aged and old people caused by chronic inflammation of the body.

This invention provides extracellular vesicle miRNAs from milk for detecting heat stress in dairy cows and their applications, belonging to the field of dairy cow health technology. This invention, through the isolation, extraction, and miRNA sequencing of extracellular vesicles derived from dairy cow milk, discovered specific miRNA molecules closely related to heat stress. Using the miRNA unconservative_3_49980 from these extracellular vesicles as a biomarker, this invention provides a simple, non-invasive, and stress-free method for detecting heat stress in dairy cows with high sensitivity. This invention also designs specific primer pairs for the miRNA, enabling the construction of a kit for early, non-invasive assessment of heat stress in dairy cows.

The invention discloses a Cordyceps militaris liquid fermentation method for increasing the yield of cordycepin, and belongs to the technical field of biological fermentation. The invention can significantly increase the yield of cordycepin in the Cordyceps militaris fermentation liquid by adding an appropriate amount of rotenone after the Cordyceps militaris is fermented for a period of time. Compared with the yield of 189.66 mg / L of traditional fermentation without adding rotenone, the yield after adding rotenone is as high as 804.69 mg / L, that is, the total yield of cordycepin is increased by 324.28%. The method has a simple process and does not need to improve the enzyme activity by means of genetic modification or the like; the production cost is low, and there is no need to continuously feed adenine and other substrates required for the synthesis of cordycepin, and the total fermentation time of cordycepin is short, and the production efficiency is significantly improved, which is suitable for industrial production.

The invention provides an adenosine production strain as well as a construction method and application thereof. After a gene for coding UDP-N-acetyl muracoyl-L-alanine-D-glutamic acid-2, 6-diamino dimer ligase in bacillus subtilis or bacillus amyloliquefaciens is modified (strengthened), the adenosine production capacity of the strain is enhanced compared with that of an unmodified strain. After the coding gene of the UDP-N-acetyl muramyl-L-alanine-D-glutamic acid-2, 6-diamino dimer ligase is introduced into a P43 promoter, the transcriptional level of MurE is improved by 46%, and the adenosine productivity is improved by 10.2%; the 252nd amino acid is mutated into isoleucine except valine, and the productivity of leucine and adenosine is improved by 5.1% and 7.7% respectively.

The application of the OsWRKY7 gene in improving the cold tolerance of rice belongs to the field of biotechnology, and discloses application of a rice transcription factor OsWRKY7 in improving the cold tolerance of rice; the genomic DNA sequence of the rice transcription factor OsWRKY7 is shown as SEQ ID NO. 1. By means of CRISPR gene targeting editing technology, the OsWRKY7 gene is knocked out or its expression is down-regulated, and a rice mutant with a function loss or down-regulated expression of the gene is obtained, which can significantly improve the cold stress tolerance. Therefore, the OsWRKY7 has a good application prospect in enhancing the cold tolerance of rice and cultivating cold-tolerant rice varieties.