Method for removing bacteria in chlamydomonas reinhardtii culture process through three mixed antibacterial agents

An antimicrobial agent and Rhine-cloth technology, which is applied in the field of removal of miscellaneous bacteria in the cultivation process of Chlamydomonas, can solve the problems of poor effect of Gram-negative bacteria and Gram-positive bacteria, etc., achieve good application prospects, inhibit The effect of self-growth and fast growth

Inactive Publication Date: 2018-09-04
XUZHOU NORMAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

Ampicillin is a semi-synthetic broad-spectrum penicillin that has a strong inhibitory effect on most Gram-positive bacteria, but is not effective against Gram-negative bacteria
Cefotaxime is a third-generation cephalosporin, which has strong antibacterial efficacy against Gram-negative bacteria and is highly stable against broad-spectrum β-lactamase produced by Gram-negative bacteria, but has little effect on Gram-positive bacteria. good

Method used

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  • Method for removing bacteria in chlamydomonas reinhardtii culture process through three mixed antibacterial agents
  • Method for removing bacteria in chlamydomonas reinhardtii culture process through three mixed antibacterial agents

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Embodiment 1

[0023] Embodiment 1: A. Inoculate Chlamydomonas that has been mixed and polluted on the TAP solid medium plate, place it at 23 ± 0.5 ° C, 14 / 10 hour light and dark photoperiod, and cultivate it for 3-5 days under the light intensity environment of 8000Lx; B. Prepare a mixed fungicide plate, which is a TAP solid medium with 20 μg / ml kresoxim-methyl, 500 μg / ml ampicillin and 100 μg / ml cefotaxime for use; C. will be cultured on the TAP solid medium The Chlamydomonas that has been mixed and contaminated is transferred and streaked onto the solid medium plate of the mixed sterilant TAP, placed at 23±0.5°C, 14 / 10 hours light and dark photoperiod, and cultivated for 5-6 days in an environment with a light intensity of 8000Lx; D .Transfer the Chlamydomonas that has been sterilized on the sterilizing agent plate to the ordinary TAP solid medium plate again, and cultivate it at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity 3 -5 days, observe whether the ste...

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Abstract

A method for removing bacteria of chlamydomonas reinhardtii through kresoxim-methyl, ampicillin and cefotaxime mixed antibacterial agents comprises the following steps: inoculating mixed and pollutedchlamydomonas through a TAP solid culture medium flat plate, and culturing; preparing a mixed antibacterial agent flat plate which is a TAP solid culture medium in which kresoxim-methyl, ampicillin and cefotaxime are added, and waiting for later use; transferring and marking the mixed and polluted chlamydomonas which is cultured on the TAP solid culture medium to the antibacterial agent mixed TAPsolid culture medium flat plate for culturing; and transferring and marking the antibacterial chlamydomonas from the antibacterial flat plate to a common TAP solid culture medium flat plate to cultureagain. According to the method, kresoxim-methyl, ampicillin and cefotaxime are used in match to effectively remove the bacteria and fungus pollution in the chlamydomonas culture process; the method has the characteristics of being high in wide popularization scope, efficient, and low in toxicity, and is extremely high in value of popularization and application in storing of precious chlamydomonasand accuracy improving of following experiments.

Description

technical field [0001] The invention relates to a method for removing bacteria and fungi from Chlamydomonas reinhardtii, specifically using the antifungal agent kresoxim-methyl, antibacterial agents ampicillin and cefotaxime to mix and remove miscellaneous bacteria during the cultivation process of Chlamydomonas reinhardtii Methods. Background technique [0002] Chlamydomonas is a kind of eukaryotic single-celled algae, known as "green yeast (green yeast)". Because of its simple structure, fast growth, short generation time, and easy cultivation and operation, it is widely used as a model organism in biological research, such as photosynthesis, phototaxis and photosensitivity of plants, regulation of plant cell cycle, plant cell apoptosis, etc. Death, chloroplast occurrence, chloroplast DNA damage and repair, cytoskeleton studies such as flagella and centrosomes, information transmission between cells, etc. In recent years, with the development and improvement of gene sequ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王亮杨丽婧陈卓雅李佳玲梁巧盈陆军郑元林
Owner XUZHOU NORMAL UNIVERSITY
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