23results about How to "Reduce pathogenicity" patented technology

According to the application of the bacteriophage to reduction of cholera transmission, the bacteriophage phi YSZKA is preserved in the China Center for Type Culture Collection on August 1, 2018, the preservation number is CCTCC NO: M 2018513, and the bacteriophage phi YSZKA is classified and named as klebsiella pneumoniae bacteriophage phi YSZKA. The method solves the problems of secondary pollution, wide transmission of vibrio cholerae and the like of the traditional split bacteriophage. The application of the bacteriophage can reduce the toxin production efficiency of vibrio cholerae in oceans, soil and sewers, thereby providing more possibilities for prevention and control of cholera epidemic situation and alleviation of cholera symptoms.

The invention provides medical application of sanguinarine in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicine. According to the invention, the flagellum of salmonella is used as a potential target for researching and developing the anti-salmonella toxicity drug, and a natural product capable of inhibiting the flagellum of salmonella is screened through a salmonella sliding motion inhibition test and a real-time fluorescent quantitative PCR test; in combination with tests such as a minimum inhibitory concentration determination test, a growth curve, inhibition of salmonella from adhesion to Caco-2 cells and the like, it is verified that sanguinarine can effectively inhibit the salmonella flagellum-mediated swimming ability on the basis of not influencing normal growth of bacteria, so that the pathogenic potential of salmonella is remarkably reduced, the effect of bacteriostasis instead of sterilization is achieved, and the application prospect is broad. A new research thought and a candidate natural compound are provided for prevention and treatment of salmonella infection, and potential practical application value is achieved.

The invention discloses a gene VII type Newcastle disease attenuated marker vaccine strain rMG7-HN-G256E, the preservation number of the gene VII type Newcastle disease attenuated marker vaccine strain is CGMCC (China General Microbiological Culture Collection Center) No.46292, and the whole-genome nucleotide sequence of the vaccine strain is as shown in SEQ ID No: 1; wherein the 256th amino acid glycine (G) of the HN protein is replaced by glutamic acid (E). A Newcastle disease virus reverse genetic manipulation platform is utilized, different sites are mutated on the basis of a Newcastle disease virus MG7 strain, a gene VII type Newcastle disease virus marker vaccine attenuated strain rMG7-HN-G256E which is low in pathogenicity, good in growth characteristic, high in virus titer, stable in passage and excellent in immunogenicity is rescued, and the marker vaccine strain is good in immunogenicity, high in immunogenicity, high in immunogenicity, high in specificity, high in specificity and high in specificity. The vaccine can induce a high-level protective antibody, completely protects immunized chickens, can be used for preventing and controlling the current epidemic gene VII type Newcastle disease virus, and lays a foundation for identifying vaccine immunity and wild virus infection.

This invention relates to the field of genetic engineering, specifically to the application of the rice blast fungus gene trs85 or its encoded protein in inhibiting the pathogenicity of rice blast fungus. Addressing the current lack of effective targets for rice blast fungus, this invention uses a high-throughput knockout system to study the effect of gene trs85 on the pathogenicity of rice blast fungus. It was found that knocking out gene trs85 inhibited autophagy in the mutant, reducing pathogenicity, thus demonstrating that protein trs85 is a potential target for rice blast control. Based on protein modeling of trs85, this invention performs virtual screening to obtain a drug that can inhibit rice blast fungus. This drug has a significant control effect on rice blast fungus, especially at a concentration of 50 μM where the control effect is optimal.

This application relates to a phage-derived small molecule polypeptide Gp04 and its application in targeting the quorum sensing LasR protein, belonging to the field of biomedical technology. The amino acid sequence of the phage-derived small molecule polypeptide Gp04 is shown in SEQ ID NO.1. The small molecule polypeptide Gp04 provided in this application can inhibit the QS-related phenotype of *Pseudomonas aeruginosa* through overexpression or exogenous addition. Bacterial two-hybrid and electrophoretic mobility assays confirmed the binding and inhibitory effect of the small molecule polypeptide Gp04 on LasR. This small molecule polypeptide can be used to prepare formulations that inhibit the virulence and biofilm formation of *Pseudomonas aeruginosa*, providing new ideas and methods for developing novel antibacterial strategies targeting QS and for phage-based co-prevention of pathogenic bacterial infections.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

The present invention relates to a device for reducing protozoa of chicken coccidiosis using a microwave field, and more particularly, to a device for reducing protozoa of chicken coccidiosis using a microwave field in order to recycle a mattress for second to fifth rearing after first rearing in a process of rearing broiler chicken. The hardened mattress is crushed by a crushing device to create a ground surface similar to a first feeding environment, and the crushed mattress is irradiated with a microwave field of a microwave device, thereby reducing or killing the number of various pathogenic bacteria such as chicken coccidiosis protozoa, viruses, bacteria, and fungi in the mattress. The broiler chicken can be healthily and safely raised by reducing the pathogenicity and the fatality rate, so that huge economic benefits are brought to poultry farms.

The invention provides application of resveratrol in preparation of drugs or feed additives for preventing and treating fish streptococcus iniae diseases. The resveratrol has the remarkable activity of inhibiting the main virulence factor phosphoglucomutase (PGM) of streptococcus iniae, and the inhibition rate of the resveratrol to the activity of the PGM at the low concentration of 10 mu g / mL reaches 27.3%. A low-concentration natural compound is used as a virulence factor inhibitor to research and develop a medicine, is more economical, green and efficient, and is expected to be researched and developed into a natural green medicine for replacing antibiotics in aquaculture.

The BcSPS1 gene and application thereof in reducing pathogenicity of Botrytis cinerea belong to the field of microbial genetic engineering technology, and the BcSPS1 gene provided by the application has a DNA sequence as shown in SEQ ID No:1 and is composed of 1947 nucleotides; the protein encoded by the BcSPS1 gene has an amino acid sequence as shown in SEQ ID No:2 and is composed of 612 amino acids; the BcSPS1 gene can be applied in the field of plant Botrytis cinerea genetic engineering; and the BcSPS1 gene for controlling pathogenicity of Botrytis cinerea can be knocked out to make the pathogenicity of Botrytis cinerea defective and can be applied in reducing pathogenicity of Botrytis cinerea.

The invention provides application of piperine in preparation of drugs and feed additives for preventing and treating vibriosis of aquatic animals, and belongs to the technical field of prevention and control of bacterial diseases of the aquatic animals. According to the invention, piperine is applied to prevention and treatment of vibriosis of aquatic animals for the first time, especially vibriosis harveyi of pearl gentian groupers, the survival rate of pearl gentian groupers infected with vibriosis harveyi can be increased within an effective dose, and the relative protection rate reaches 49.99%.

The invention discloses a FolRtt109 gene targeting agent and an application of the FolRtt109 gene targeting agent in inhibition of phytopathogen. In the fusarium oxysporum, the pathogenicity of the fusarium oxysporum can be obviously inhibited by knocking out the gene FolRtt109, and the gene has a crucial relationship with the pathogenicity of the fusarium oxysporum. The gene is knocked out by using a homologous recombination method to obtain a gene knockout mutant, and the pathogenicity of the mutant to tomatoes is remarkably reduced. Experiments prove that by applying the FolRtt109 gene targeted medicament CID-4785700, infection of various phytopathogens such as fusarium oxysporum, botryis cinerea, fusarium graminearum and magnaporthe oryzae can be remarkably inhibited, the purpose of preventing and treating the various phytopathogens can be achieved, and the FolRtt109 gene targeted medicament CID-4785700 is simple in use method, free of influence on plant growth, safe, reliable and suitable for popularization and application. Wide application prospects are realized.

The present invention discloses the application of the AST2 gene of banana wilt pathogen in regulating the pathogenicity of banana wilt pathogen. The present invention utilizes the principle of homologous recombination to knock out the gene FocAST2; and utilizes the method of PEG-mediated protoplast transformation to obtain the knockout body. The results show that the knockout of the FocAST2 gene causes mycelium to curl and other growth deformities, significantly affects the spore production of the strain, reduces the content of α-ketoglutaric acid (α-KG), and increases the H 2 O 2 The content increased and the pathogenicity decreased, indicating that knocking out the FocAST2 gene affected the growth of banana wilt pathogen and reduced its pathogenicity. The present invention helps to further clarify the pathogenic molecular mechanism of banana wilt pathogen and provides a target gene for the development of effective fungicides.

The invention provides a porcine rotavirus G5 type and application thereof. According to the present invention, the porcine rotavirus G5 type SC05 strain is separated from the porcine intestinal tract tissue, and is subjected to passage purification to obtain the porcine rotavirus G5 type SC05 strain, and the microbial preservation number of the porcine rotavirus G5 type SC05 strain is CGMCC No.46179; the isolated strain can stably proliferate on passage cells to generate typical cytopathy. The G5 type isolated strain of the porcine rotavirus disclosed by the invention has excellent immunogenicity; the vaccine prepared from the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, the ELISA kit prepared from the isolated strain can be used for well detecting the porcine rotavirus G5 type antibody in serum.

This invention belongs to the field of biological control of plant diseases, specifically relating to a virulent strain of *Botrytis cinerea* SdBd-6 and its applications. The virulent strain of *Botrytis cinerea* SdBd-6 is classified and named... Botryosphaeria dothidea The attenuated strain SdBd-6 of Staphylococcus aureus was deposited at the Guangdong Provincial Center for Microbial Culture Collection on January 14, 2026, with accession number GDMCC: 67656. Walsa money The growth of the fungus has a certain inhibitory function. At the same time, after 10 generations of continuous inoculation, the virus contained in the attenuated strain SdBd-6 of *Botrytis cinerea* was not lost, and its growth characteristics and pathogenicity remained attenuated strain characteristics. This provides materials and lays the foundation for the green control of plant fungal diseases caused by the pathogenic fungus *Botrytis cinerea*.

The invention discloses a gene VII type Newcastle disease attenuated marker vaccine strain rMG7-F-N471A, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.46293. The whole-genome nucleotide sequence of the vaccine strain is as shown in SEQ ID No: 1; wherein the 471st amino acid asparagine (N) of the F protein is replaced with alanine (A). A Newcastle disease virus reverse genetic manipulation platform is utilized, different sites are mutated on the basis of a Newcastle disease virus MG7 strain, a gene VII type Newcastle disease virus marker vaccine attenuated strain rMG7-F-N471A which is low in pathogenicity, good in growth characteristic, high in virus titer, stable in passage and excellent in immunogenicity is rescued, and the marker vaccine strain is good in immunogenicity, high in immunogenicity, high in immunogenicity, high in specificity, high in specificity, high in specificity and high in specificity. The vaccine can induce a high-level protective antibody, completely protects immunized chickens, can be used for preventing and controlling the current epidemic gene VII type Newcastle disease virus, and lays a foundation for identifying vaccine immunity and wild virus infection.

The invention discloses a gene VII type Newcastle disease attenuated marker vaccine strain rMG7-L-V1291I, the preservation number of the gene VII type Newcastle disease attenuated marker vaccine strain rMG7-L-V1291I is CGMCC (China General Microbiological Culture Collection Center) No.46291, and the whole-genome nucleotide sequence of the vaccine strain is as shown in SEQ ID No: 1; wherein the 1291th amino acid valine (V) of the L protein is replaced by isoleucine (I). A Newcastle disease virus reverse genetic manipulation platform is utilized, different sites are mutated on the basis of a Newcastle disease virus MG7 strain, a gene VII type Newcastle disease virus marker vaccine attenuated strain rMG7-L-V1291I which is low in pathogenicity, good in growth characteristic, high in virus titer, stable in passage and excellent in immunogenicity is rescued, and the marker vaccine strain is good in immunogenicity, high in immunogenicity, high in specificity, high in immunogenicity and capable of being used as a marker vaccine for the Newcastle disease virus. The vaccine can induce a high-level protective antibody, completely protects immunized chickens, can be used for preventing and controlling the current epidemic gene VII type Newcastle disease virus, and lays a foundation for identifying vaccine immunity and wild virus infection.

The invention discloses a sclerotinia sclerotiorum gene SsCRZ1 and application thereof in regulation and control of infection pad formation and pathopoiesis, and belongs to the technical field of microbial genetic engineering. The DNA sequence of the gene SsCRZ1 is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene SsCRZ1 is as shown in SEQ ID NO: 2. Gene knockout and refilling experiments prove that the infection pad forming ability of sclerotinia sclerotiorum and the pathogenicity of sclerotinia sclerotiorum to host plants can be remarkably weakened by deletion of the SsCRZ1 gene, and the functions can be recovered by refilling the gene. Therefore, the SsCRZ1 gene is a gene necessary for formation and pathopoiesis of a sclerotinia sclerotiorum infection pad. According to the invention, the function of the SsCRZ1 gene in sclerotinia sclerotiorum is defined for the first time, and a theoretical basis is provided for the gene as a new target for prevention and control of sclerotiniose.

The invention relates to a method for knocking out Fusarium graminearum FgSCP43 gene, and a mutant strain delta FgSCP43 with the FgSCP43 gene knocked out, which is obtained by the method. The FgSCP43 gene is knocked out from the fusarium graminearum, so that the spore yield of the fusarium graminearum is reduced, meanwhile, the pathogenicity to wheat coleoptiles and wheat ears is remarkably reduced, and the FgSCP43 plays an important role in the pathogenic process of the fusarium graminearum. The invention provides a new thought and direction for preventing and treating wheat scab.

The invention discloses an application of 2-aminoquinoline in preparation of drugs for inhibiting salmonella typhimurium. 2-aminoquinoline is combined with LsrB protein to inhibit expression of QS-related genes and virulence genes and inhibit formation of a biofilm, so that the pathogenicity of salmonella typhimurium is reduced, and the application of 2-aminoquinoline in preparation of drugs for inhibiting salmonella typhimurium is realized. And the 2-aminoquinoline can inhibit invasion and adhesion of salmonella typhimurium to cells, so that the 2-aminoquinoline can be applied to preparation of salmonella typhimurium inhibiting drugs.

The application belongs to the technical field of biological control of agriculture, and particularly relates to a DNA pesticide as well as a preparation method and application thereof. The application provides a DNA pesticide, which comprises a nano-carrier and a genome of a fungal virus; the fungal virus is a plant pathogenic fungal virus, and can cause pathogenicity decline of a host fungus. The application uses the nano-carrier to load the genomic DNA of the fungal virus, so as to deliver the genomic DNA of the fungal virus to a plant pathogenic fungus, make the genomic DNA of the fungal virus replicate, transcribe and translate in the plant pathogenic fungus, and form a mature virus, thereby causing pathogenicity decline of the plant pathogenic fungus, and achieving the prevention and treatment of fungal diseases.

This invention discloses a pathogenic gene, protein, and application of *Verticillium dahliae*. This invention relates to the field of biotechnology and provides a method for controlling *Verticillium dahliae* wilt based on RNAi (RNA interference), along with its related components and applications. Specifically, it discloses the method of inhibiting or silencing the pathogenic gene of *Verticillium dahliae*. VdHDAC The application of the expression method (whose encoded protein is shown in SEQ ID NO:3) for the prevention and control of Verticillium wilt relies on providing a class of dsRNA molecules that can target this gene, with the positive strand preferably selected from SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:11. Based on this molecule, this invention further provides recombinant vectors containing its coding sequence, recombinant microorganisms, transgenic disease-resistant plants, and RNAi pesticide formulations. These methods can be achieved through host-induced gene silencing, microorganism-mediated gene silencing, or formulation treatment, effectively reducing the virulence of the pathogen and providing a new technical approach for the green control of Verticillium wilt.

The invention discloses application of a fungal virus in prevention and treatment of pear tree canker and apple tree canker. The mycovirus is a positive-sense single-stranded RNA virus, and the cDNA nucleotide sequence of the mycovirus is shown as SEQ ID No. 1. The fungus containing the virus is valsa pear tree rot pathogen HJL1-22 (gamma), the fungus is infected by the fungal virus in the claim 1, the name of the fungus is HJL1-22 (gamma), the classification name of the fungus is Valsa pyri, and the preservation number of the fungus in the China General Microbiological Culture Collection Center is CGMCC No.41449. The virus Vphv1-gamma can be secreted out of cells, so that a culture solution without mycelia can be used as a biocontrol agent for preventing and treating the pear tree canker and the apple tree canker, and pathopoiesis and diffusion of the pear tree canker and the apple tree canker are effectively weakened or inhibited; the application has the prospect of biologically preventing and treating the pear tree canker and the apple tree canker in production practice.