8results about How to "Reduce pathogenicity" patented technology

This invention relates to the field of genetic engineering, specifically to the application of the rice blast fungus gene trs85 or its encoded protein in inhibiting the pathogenicity of rice blast fungus. Addressing the current lack of effective targets for rice blast fungus, this invention uses a high-throughput knockout system to study the effect of gene trs85 on the pathogenicity of rice blast fungus. It was found that knocking out gene trs85 inhibited autophagy in the mutant, reducing pathogenicity, thus demonstrating that protein trs85 is a potential target for rice blast control. Based on protein modeling of trs85, this invention performs virtual screening to obtain a drug that can inhibit rice blast fungus. This drug has a significant control effect on rice blast fungus, especially at a concentration of 50 μM where the control effect is optimal.

This application relates to a phage-derived small molecule polypeptide Gp04 and its application in targeting the quorum sensing LasR protein, belonging to the field of biomedical technology. The amino acid sequence of the phage-derived small molecule polypeptide Gp04 is shown in SEQ ID NO.1. The small molecule polypeptide Gp04 provided in this application can inhibit the QS-related phenotype of *Pseudomonas aeruginosa* through overexpression or exogenous addition. Bacterial two-hybrid and electrophoretic mobility assays confirmed the binding and inhibitory effect of the small molecule polypeptide Gp04 on LasR. This small molecule polypeptide can be used to prepare formulations that inhibit the virulence and biofilm formation of *Pseudomonas aeruginosa*, providing new ideas and methods for developing novel antibacterial strategies targeting QS and for phage-based co-prevention of pathogenic bacterial infections.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

This invention belongs to the field of biological control of plant diseases, specifically relating to a virulent strain of *Botrytis cinerea* SdBd-6 and its applications. The virulent strain of *Botrytis cinerea* SdBd-6 is classified and named... Botryosphaeria dothidea The attenuated strain SdBd-6 of Staphylococcus aureus was deposited at the Guangdong Provincial Center for Microbial Culture Collection on January 14, 2026, with accession number GDMCC: 67656. Walsa money The growth of the fungus has a certain inhibitory function. At the same time, after 10 generations of continuous inoculation, the virus contained in the attenuated strain SdBd-6 of *Botrytis cinerea* was not lost, and its growth characteristics and pathogenicity remained attenuated strain characteristics. This provides materials and lays the foundation for the green control of plant fungal diseases caused by the pathogenic fungus *Botrytis cinerea*.

The invention discloses a sclerotinia sclerotiorum gene SsCRZ1 and application thereof in regulation and control of infection pad formation and pathopoiesis, and belongs to the technical field of microbial genetic engineering. The DNA sequence of the gene SsCRZ1 is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene SsCRZ1 is as shown in SEQ ID NO: 2. Gene knockout and refilling experiments prove that the infection pad forming ability of sclerotinia sclerotiorum and the pathogenicity of sclerotinia sclerotiorum to host plants can be remarkably weakened by deletion of the SsCRZ1 gene, and the functions can be recovered by refilling the gene. Therefore, the SsCRZ1 gene is a gene necessary for formation and pathopoiesis of a sclerotinia sclerotiorum infection pad. According to the invention, the function of the SsCRZ1 gene in sclerotinia sclerotiorum is defined for the first time, and a theoretical basis is provided for the gene as a new target for prevention and control of sclerotiniose.

The application belongs to the technical field of biological control of agriculture, and particularly relates to a DNA pesticide as well as a preparation method and application thereof. The application provides a DNA pesticide, which comprises a nano-carrier and a genome of a fungal virus; the fungal virus is a plant pathogenic fungal virus, and can cause pathogenicity decline of a host fungus. The application uses the nano-carrier to load the genomic DNA of the fungal virus, so as to deliver the genomic DNA of the fungal virus to a plant pathogenic fungus, make the genomic DNA of the fungal virus replicate, transcribe and translate in the plant pathogenic fungus, and form a mature virus, thereby causing pathogenicity decline of the plant pathogenic fungus, and achieving the prevention and treatment of fungal diseases.

This invention discloses a pathogenic gene, protein, and application of *Verticillium dahliae*. This invention relates to the field of biotechnology and provides a method for controlling *Verticillium dahliae* wilt based on RNAi (RNA interference), along with its related components and applications. Specifically, it discloses the method of inhibiting or silencing the pathogenic gene of *Verticillium dahliae*. VdHDAC The application of the expression method (whose encoded protein is shown in SEQ ID NO:3) for the prevention and control of Verticillium wilt relies on providing a class of dsRNA molecules that can target this gene, with the positive strand preferably selected from SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:11. Based on this molecule, this invention further provides recombinant vectors containing its coding sequence, recombinant microorganisms, transgenic disease-resistant plants, and RNAi pesticide formulations. These methods can be achieved through host-induced gene silencing, microorganism-mediated gene silencing, or formulation treatment, effectively reducing the virulence of the pathogen and providing a new technical approach for the green control of Verticillium wilt.