9 results about "Acetolactate decarboxylase" patented technology

The present application belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered strain with high yield of acetoin and 2,3-butanediol, a construction method and application. The present application obtains acoR deletion mutant MW03-ΔA by knocking out the regulatory gene acoR of the acetoin dehydrogenase enzyme system, blocks the degradation of AoDHES to acetoin, effectively reduces the production of by-products, thereby facilitating the accumulation of more acetoin. A strong promoter expression vector is further constructed in the MW03-ΔA strain, and the alpha-acetyl lactate decarboxylase gene alsD is connected, and then introduced into the acoR deletion mutant MW03-ΔA of Bacillus licheniformis, so as to overexpress alpha-acetyl lactate decarboxylase, and obtain a genetically engineered strain MW-BL4 with high yield of acetoin and 2,3-butanediol. The present application has the beneficial effect that compared with a single product of the engineered strain, the present application can realize the production of acetoin and 2,3-butanediol and other multiple products by using a single strain, and the yield is considerable, which can significantly reduce the cost of bacterial strains in industrial production and improve the production efficiency.

This invention belongs to the field of agricultural biotechnology and discloses a low-temperature synergistic biological agent, its preparation method, and its application. The core innovation lies in using a unique engineered Bacillus subtilis strain as the core functional carrier, and performing a ternary targeted compounding with polydeoxyribonucleotides (PDRN) and biostimulants to achieve a triple synergistic low-temperature resistance effect. The engineered Bacillus subtilis strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2026, with accession number CGMCC No. 37899. The chassis strain of Bacillus subtilis was deposited at the same center on March 3, 2026, with accession number CGMCC No. 37834. The modification strategy involves knocking out the α-subunit gene of acetoin dehydrogenase and the phosphoacetyltransferase gene from the genome of Bacillus subtilis CGMCC No. 37834, and expressing the acetyllactate synthase gene and the α-acetyllactate decarboxylase gene.

The invention provides a genetically engineered recombinant Klebsiella michiganensis having reduced or inactivated glucose-specific PTS transport system, glycerol kinase, glycerol dehydrogenase, alcohol dehydrogenase, acetolactate synthase, acetolactate decarboxylase, fumarate reductase and 3-phosphoglyceraldehyde dehydrogenase, having, preferably enhanced, NADPH-dependent alcohol dehydrogenase, diol dehydratase, 3-phosphoglycerate dehydrogenase and 3-phosphoglycerate phosphatase activities, methods of making said recombinant Klebsiella michiganensis, and uses and methods of producing 1,3-propanediol using said recombinant Klebsiella michiganensis.

The present invention relates to novel stable forms of acetolactate decarboxylase (ALDC) enzymes that have improved stability in the presence of proteases. More particularly, the present invention relates to an improved brewing process in which a stabilized ALDC is used in conjunction with a proline-specific endoprotease during beer fermentation to provide a less off-flavor, colloid-stabilized beer in a shortened time.

Provided herein is a genetically engineered microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus. Replacement of the acetolactate decarboxylase gene with DNA encoding one or more native or nonnative enzymes confers certain advantages, including fermentation stability and increased production of native and nonnative products from gaseous substrates.

The invention discloses klebsiella pneumoniae for efficiently producing D-pantothenic acid as well as a construction method and application of the klebsiella pneumoniae, and the specific construction method comprises the following steps: by taking wild klebsiella pneumoniae as an original strain, overexpressing a ketopantoic acid hydroxymethyltransferase gene panB, a pantothenic acid synthetase gene panC and a ketopantoic acid reductase gene panE from escherichia coli; the invention relates to related genes for weakening the expression of a valine-pyruvate transaminase gene ilvE and inactivating byproduct synthesis in a host, and the related genes comprise an alpha-acetolactate decarboxylase gene budA, an acetoin reductase gene budC, glycerol dehydrogenase gldA, a pyruvate oxidase gene poxB, a pyruvate formate lyase activating enzyme gene pflB, an ethanol dehydrogenase gene adhE and a lactic dehydrogenase gene ldhA. The constructed klebsiella pneumoniae engineering bacterium can efficiently synthesize D-pantothenic acid by fermenting glucose, and has great application potential and economic value.

The application provides an engineered halomonas and a method for producing acetoin, wherein the halomonas expresses acetyl-lactate synthase and acetyl-lactate decarboxylase, and does not express PHA hydratase and acetoacetyl-CoA or the expressed PHA hydratase and acetoacetyl-CoA are not functional or have reduced function. The engineered halomonas can realize economic, sustainable and efficient production of acetoin, and improve single batch fermentation yield.

The invention belongs to the technical field of gene engineering, and particularly relates to a gene engineering strain for high yield of acetoin and 2, 3-butanediol, a construction method and application. According to the invention, an acoR deletion mutant strain MW03-delta A is obtained by knocking out a regulatory gene acoR of an acetoin dehydrogenase enzyme system, the degradation of AoDHES on acetoin is blocked, and the generation of byproducts is effectively reduced, so that more acetoin is favorably accumulated, a strong promoter expression vector is further constructed in the MW03-delta A strain, and an alpha-acetolactate decarboxylase gene alsD is connected and coded, so that the acetoin dehydrogenase is obtained. The method comprises the following steps: by taking acetoin and 2, 3-butanediol as raw materials, introducing the raw materials into a bacillus licheniformis acoR deletion mutant MW03-delta A, so as to overexpress alpha-acetolactate decarboxylase, thereby obtaining a genetic engineering strain MW-BL4 for high yield of acetoin and 2, 3-butanediol. The method has the beneficial effects that compared with an engineering strain of a single product, the production of various products such as acetoin, 2, 3-butanediol and the like can be realized by utilizing one strain, the yield is considerable, the strain cost can be obviously reduced in industrial production, and the production benefit can be improved.

The application belongs to the technical field of synthetic biology, and a bacillus subtilis engineering bacterium is preserved in the China General Microbiological Culture Collection Center on March 11, 2026, with a preservation number of CGMCC No. 37899. The chassis strain bacillus subtilis is preserved in the China General Microbiological Culture Collection Center on March 3, 2026, with a preservation number of CGMCC No. 37834. The modification strategy is to knock out the alpha subunit gene of acetoin dehydrogenase and the phosphotransferase gene from the genome of bacillus subtilis CGMCC No. 37834, and express the acetolactate synthase gene and the alpha-acetolactate decarboxylase gene. The engineering bacterium can be used to prepare fermentation products containing the microbial-crop interaction signal substances acetoin and 2,3-butanediol, and can be used to promote the growth of crop roots, improve the growth and yield of crops.