The invention discloses Bacillus vallismortis and application thereof in vinegar brewing. The name of the strain is Bacillus vallismortis QH-20001, the preservation number is CGMCC No.22251, the preservation date is April 27, 2021, the strain is preserved in the China General Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3 , No. 1 Yard, Beichenxi Road, Chaoyang District, Beijing. The Bacillus vallismortis is applied to cellar vinegar brewing, can grow and metabolize under an acidic condition and produce alpha-acetolactate decarboxylase, the step of oxidizing alpha-acetolactate into diacetyl and then reducing the diacetyl to obtain acetoin can be avoided, and the alpha-acetolactate is quickly converted into the acetoin, thus, sufficient premise substances of ligustrazine is provided, the content of the acetoin and the ligustrazine in cellar vinegar can be obviously increased, and the production amount of the diacetyl is balanced.