63 results about "Ferredoxin" patented technology

The invention provides a method of improving disease resistance by transforming arethusa with longan ferredoxin gene and belongs to the technical field of genetic engineering. The longan ferredoxin gene (Dlfd3) (Register number: JF733784) is guided to arethusa oncidium by means of a transgenic technology, and the obtained transformed plant represents higher transplanting survival rate and disease resisting capability. Compared with wild plants, the soft rot resistant capability of the transgenic plant is improved from high touch soft rot (Di=100) to intermediate soft rot resistance (Di=27.5). The transplanting survival rate is improved from 79.3% to 95.7%, thereby realizing extremely significant difference.

The invention discloses a method for promoting synthesis of bacillus subtilis acetylglucosamine and belongs to the field of genetic engineering. The method is characterized in that a recombinant bacillus subtilis BSGNKAP2 is taken as a starting strain, and pyruvate carboxylase BalpycA from bacillus cereus is exogenously introduced, so that metabolism overflow of bacillus subtilis central carbon iseliminated and synthesis of acetoin as a byproduct is avoided; furthermore, five exogenous reductive metabolism reactions are introduced to replace a glycolytic pathway and a reaction of producing NADH in tricarboxylic acid cycle and reconstruct intracellular reductive metabolism, wherein the five exogenous reductive metabolism reactions specifically comprise glyceraldehydes-3-ferredoxin phosphate dehydrogenas, isocitric acid NAD<+> dehydrogenas, malic acid quinone dehydrogenas, ketonic acid ferredoxin oxidordeuctase and nitrogenase ferritin. In the shake-flask fermentation process of using acomplex medium, the yield of a recombinant strain BSGNKAP8 acetylglucosamine is 24.50g / L, and the yield of acetylglucosamine / glucose is 0.469g / L, which are 1.97 times and 2.13 times that of the starting strain BSGNKAP2.

The present invention relates to the base sequence of whole genes involved in the biosynthesis of FR-008 polyketides derived from Streptomyces sp. FR-008. This base sequence comprises genes coding for ketosynthase (KS), acyl transferase (AT), acyl carrier protein (ACP), ketoreductase (KR), dehydratase (DH) and enoyl reductase (ER) domains, and genes coding for modifier enzymes, such as ABC transporter, cytochrome P450 monooxygenase, ferredoxin, thioesterase, sugar synthetic protein, FAD-dependent monooxygenase, 4-amino-4-deoxychorismate (ADC) synthase and ADC lyase. The gene base sequence according to the present invention can be used to increase the productivity of the existing FR-008 polyketides or produce new FR-008 polyketides, through modification of its parts.

An isolated polypeptide comprising a hydrogen generating enzyme attached to a heterologous ferredoxin is disclosed, as well as polynucleotides encoding same, nucleic acid constructs capable of expressing same and cells expressing same. A method for generating hydrogen using the isolated polypeptide is also disclosed.

In one aspect, the present invention is directed to a method for preventing or treating necrotic enteritis by administering a hyperimmunized egg product obtained from an egg-producing animal to an avian. The hyperimmunized egg product may contain an antibody specific to an antigen selected from the group consisting of Clostridium perfringens α-toxin, Clostridium perfringens elongation factor Tu (EF-Tu), Clostridium perfringens necrotic enteritis B-like (NetB) toxin, Clostridium perfringens Pyruvate: Ferredoxin oxidoreductase (PFO), and Eimeria tenella elongation factor 1-alpha.

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, cozymase A, oxidized form ferredoxin, longchain-acyl-cozymase A, homocysteine desulfhydrase, 2-oxobutyryl synthetase, fatty acyl-CoA synthetase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.

The invention discloses a method for promoting the synthesis of acetylglucosamine of bacillus subtilis, which belongs to the field of genetic engineering. The present invention uses the recombinant Bacillus subtilis BSGNKAP2 as the starting strain, and exogenously introduces pyruvate carboxylase BalpycA derived from Bacillus cereus to eliminate the overflow of carbon metabolism in the center of Bacillus subtilis, avoid the synthesis of by-product acetoin, and further Introduce 5 exogenous reducing power metabolism reactions to replace the glycolysis pathway, the reaction of NADH in the tricarboxylic acid cycle, and reconstruct the intracellular reducing power metabolism, including glyceraldehyde-3-phosphate ferredoxin dehydrogenase, iso CitrateNAD + Dehydrogenase, malate quinone dehydrogenase, ketoferredoxin oxidoreductase, and nitrogenase ferritin. During the shake flask fermentation using the compound medium, the yield of acetylglucosamine of the recombinant strain BSGNKAP8 was 24.50g / L, and the yield of acetylglucosamine / glucose was 0.469g / g, which were 1.97 and 2.13 times that of the starting strain BSGNKAP2, respectively.

Compositions and methods for improving plant growth are provided herein. The maize RbcS7A promoter is provided which is a developmentally regulated promoter. Polynucleotides encoding ferredoxin-thioredoxin reductase proteins, polypeptides encompassing ferredoxin-thioredoxin reductase proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.