The invention discloses a tissue culture propagation method for butterfly oncidium (Pschopsis) seedlings, aiming to provide a method for fast propagation, which can obtain a large number of test-tube seedlings in a short period of time, does not cause damage to the mother plant, and can maintain the excellent quality of the mother plant. Tissue culture propagation methods for traits of Phalaenopsis oncidium. The method comprises the steps of selecting explants with sectioned flower stems of excellent strains of Oncidium phalaenopsis with vigorous growth, sterilizing the explants, inducing adventitious buds in the pedicel seedling induction medium, and then removing the pedicel seedling stem tip Insert the protocorm induction and proliferation medium to carry out the induction and subculture of the protocorm, then carry out the differentiation of the protocorm on the protocorm differentiation medium, and then inoculate the seedlings that can be differentiated from the protocorm on the strong seedling medium for cultivation, When the test-tube seedlings grow to 3-4 cm high, harden the seedlings under natural light in the greenhouse for 7-14 days, take out the test-tube seedlings, wash off the root medium, and plant them in the mixed substrate of bark, blue stone and peat, and further Cultivated into seedlings, the survival rate of transplanting is 90-98%. The invention opens up a new way for the breeding of oncidium phalaenopsis seedlings, and can provide a large amount of oncidium phalaenopsis seedlings for the market.