Rapid Propagation Method of High Quality Seedling of Butterfly Oncidium

A technology for oncidium phalaenopsis and seedlings, which is applied in the field of tissue culture and test-tube propagation of oncidium phalaenopsis seedlings, can solve the problems of no patents for tissue culture of oncidium phalaenopsis seedlings, and achieve low investment, high output, Good seedling quality

Active Publication Date: 2011-12-14
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Effect test

Embodiment 1

[0024] (1) When flowering, select the knotted pedicels of the vigorously growing Devil Oncidium (Psychopsis papilio) as explants, soak the explants with 70% alcohol for 60 seconds, and then sterilize them with 0.1% mercuric chloride solution for 20 minutes , rinsed with sterile water for 4 times, inoculated on the pedicel seedling induction medium and cultured, and the pedicel seedlings could be induced in 40 days. The pedicel seedling induction medium contains 1 g of Huabao No. 1 (HYPONeX 1) per liter, 80 mg of inositol, 1.5 mg of glycine, 0.05 mg of thiamine hydrochloride (VB1), 0.4 mg of pyridoxine hydrochloride (VB6), tobacco Acid 0.4mg, sodium citrate 50mg, naphthaleneacetic acid (NAA) 0.2mg, 10% coconut milk, sucrose 15g, agar 7g.

[0025] (2) The seedlings induced by the pedicels are transferred to the protocorm-like induction medium for cultivation, and the formation of the protocorm-like can be induced in 60 days, and the proliferation of the protocorm-like can be car...

Embodiment 2

[0031] (1) When flowering, select the knotted pedicels of Psychopsis versteegiana, which grow robustly, as explants, soak the explants with 75% alcohol for 45 seconds, and then sterilize with 0.15% mercuric chloride solution After 15 minutes, rinsing with sterile water for 5 times, it is inoculated on the pedicel seedling induction medium for cultivation, and the pedicel seedling can be induced to produce in 45 days. The pedicel seedling induction medium contains 1.5g of Huabao No. 1 (HYPONeX 1) per liter, 100mg of inositol, 2mg of glycine, 0.1mg of thiamine hydrochloride (VB1), 0.6mg of pyridoxine hydrochloride (VB6), tobacco Acid 0.6mg, sodium citrate 75mg, naphthaleneacetic acid (NAA) 0.5mg, 15% coconut milk, sucrose 20g, agar 6.5g.

[0032](2) The seedlings induced by the pedicels are transferred to the protocorm-like induction medium for cultivation, and the formation of the protocorm-like can be induced in 50 days, and the proliferation of the protocorm-like can be carri...

Embodiment 3

[0038] (1) When blooming, select the stalked pedicels of the healthy and robust oncidium (Psychopsis Carolina Yellow Butterfly) as explants, soak the explants in 80% alcohol for 30 seconds, and then use 0.2% mercuric chloride solution After being sterilized for 10 minutes, rinsed with sterile water for 6 times, it is inoculated on the pedicel seedling induction medium for cultivation, and the pedicel seedlings can be induced to produce in 30 days. The pedicel seedling induction medium contains 2 g of Huabao No. 1 (HYPONeX 1) per liter, 120 mg of inositol, 2 mg of glycine, 0.2 mg of thiamine hydrochloride (VB1), 0.8 mg of pyridoxine hydrochloride (VB6), niacin 0.8mg, sodium citrate 100mg, naphthaleneacetic acid (NAA) 1mg, 20% coconut milk, sucrose 30g, agar 6g.

[0039] (2) The seedlings induced by the pedicels are transferred to the protocorm-like induction medium for cultivation, the formation of the protocorm-like can be induced in 40 days, and the proliferation of the proto...

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Abstract

The invention discloses a tissue culture propagation method for butterfly oncidium (Pschopsis) seedlings, aiming to provide a method for fast propagation, which can obtain a large number of test-tube seedlings in a short period of time, does not cause damage to the mother plant, and can maintain the excellent quality of the mother plant. Tissue culture propagation methods for traits of Phalaenopsis oncidium. The method comprises the steps of selecting explants with sectioned flower stems of excellent strains of Oncidium phalaenopsis with vigorous growth, sterilizing the explants, inducing adventitious buds in the pedicel seedling induction medium, and then removing the pedicel seedling stem tip Insert the protocorm induction and proliferation medium to carry out the induction and subculture of the protocorm, then carry out the differentiation of the protocorm on the protocorm differentiation medium, and then inoculate the seedlings that can be differentiated from the protocorm on the strong seedling medium for cultivation, When the test-tube seedlings grow to 3-4 cm high, harden the seedlings under natural light in the greenhouse for 7-14 days, take out the test-tube seedlings, wash off the root medium, and plant them in the mixed substrate of bark, blue stone and peat, and further Cultivated into seedlings, the survival rate of transplanting is 90-98%. The invention opens up a new way for the breeding of oncidium phalaenopsis seedlings, and can provide a large amount of oncidium phalaenopsis seedlings for the market.

Description

technical field [0001] The invention relates to a breeding method of Butterfly Oncidium. Specifically, it relates to a method for tissue culture test tube propagation of Oncidium phalaenopsis seedlings. Background technique [0002] Butterfly oncidium, the general term for plants of the genus Psychopsis and their hybrids, native to Central and South America, distributed in Tenet Island, Colombia, Costa Rica, Peru, Panama and other places, mostly as Rare orchid potted ornamental. There are 5 native species and more than 50 hybrid species in this genus. Butterfly oncidium is a kind of orchid with unique shape and high ornamental value in Orchidaceae. Its inflorescence is an infinite inflorescence, which blooms all the year round. After one flower fades, another blooms. The flower shape of butterfly oncidium resembles a bright butterfly. The slender petals are like the long tentacles of the butterfly, and the side sepals are like the wings with chloasma. The flowering perio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曾宋君陈之林段俊吴坤林张建霞李冬梅
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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