76results about How to "Seedlings of good quality" patented technology

The present invention relates to the quick breeding method of high quality seedling of Phalaenopsis, known as 'queen orchid' and the culture medium therewith. The present invention uses the flower stem section of Phalaenopsis as explant and induces flower stem seedling with unique culture medium and performs proliferation with fascicular bud stem tip or stem section to produce high quality seedling of Phalaenopsis. The method is simple, practical, feasible and high in application value.

The invention provides a multi-flower paphiopedilum test-tube seedling cultivation method, belonging to the technical field of plant growing. The method is characterized by comprising the following steps: 1) pod acquisition; 2) aseptic seeding; 3) multiplication subculture; 4) root and strong seedling culture; and 5) test-tube seedling transplantation. The multi-flower paphiopedilum test-tube seedling cultivation method has the characteristics of high germination rate, short seedling emergence cycle, robust seedlings, developed root systems, good seedling quality, low production cost and the like, can be used for large-scale industrialized production of paphiopedilum and has a good application prospect.

The invention provides a method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings, which comprises the following steps of: performing artificial pollination to obtain hybrid seeds; picking off the capsule when the capsule has not reached full maturity; disinfecting the surface of the capsule and aseptically sowing the capsule; culturing the capsule in the dark and sprouting to obtain the rhizome; inoculating the rhizome into a bud induction culture medium; inducing bud through the rhizome, rooting and culturing the sprout; and transplanting test-tube plantlet and the like. The method ensures high germination of the cymbidium goeringii hybrid seeds, short period for raising the seedlings and good quality of the seedlings by using three culture media optimized by sifting. The complete set of method of the invention has high operability, well solves the problems of extremely difficult germination of the cymbidium goeringii hybrid seeds under natural conditions due to the incomplete embryonic development and has high application value and popularization value.

Disclosed is the high quality germchit tissure culturing and rapid breeding method of Dendrobium sp., wherein a large amount of test tube seedlings with stabilized performance are obtained through fine breed tissue culture by adopting the path of clumping buds. The produced Dendrobium sp. has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and fine growth status.

The invention discloses a ficus tikoua rapid propagation method. According to the method, ficus tikoua terminal buds or axillary buds with stem sections are cultured in an initial medium, such that aseptic seedlings are obtained; clustered buds are induced on a proliferation medium; and rooting is carried out on a rooting medium. When ficus tikoua tissue culture is carried out with the set of technical methods provided by the invention, the period is short, propagation coefficient is high, cost is low, and high factory production capacity is provided. When propagation is carried out with the method provided by the invention, ficus tikoua can be effectively protected, developed, and utilized.

The present invention relates to the sterilized seeding and tissue cultivation technology of Vanda as one of four main kinds of ornamental orchid. The present invention obtains great amount of test seedling of Vanda through sterilized seeding in artificial culture medium, and forms complete plant via proliferation and rooting culture of stem tip or stem node of abacterial seedling. The present invention has practical operation and high application value.

The invention relates to the technical field of non-toxic cultivation of plants, in particular to a phalaenopsis non-toxic seedling cultivation and propagation method. The phalaenopsis non-toxic seedling cultivation and propagation method comprises the following steps: performing heat treatment on the plants, selecting materials, disinfecting the materials, performing pre-treatment on the materials, and performing tissue cultivation. The tissue cultivation comprises protocorm induced cultivation, multiplication cultivation, strong seedling cultivation and transplanting. After the phalaenopsis seedlings are cultivated according to the method, the disease incidence rate is low, the survival seedling rate is high, the seedling quality is high, the adaptability of the seedlings is high after being planted, the seedlings grow well, the requirements on commercialized production are met in a short time through a biotechnological means, and technical support is provided for large-scale and industrialized cultivation of the phalaenopsis non-toxic seedlings of enterprises.

The present invention relates to the abacterial seeding and tissue culture of Dendrobium unicum. The Dendrobium as important orchid family has about 1000 varieties, and Dendrobium unicum is beautiful and unique and has very high enjoying value. Up to now, Dendrobium unicum is propagated via offshoot process in slow rate and is hard to germinate naturally owing to its incomplete development of embryo. The present invention obtains great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding and small seedling proliferation and obtains complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

The invention discloses a method for aseptically sowing and rapidly propagating paphiopedilum helenae. The method is characterized by taking seeds in appropriate embryonic age as explants, aseptically sowing the seeds, differentiating protocorms, proliferating protocorm-like bodies and culturing strong seedlings on a unique culture medium, and producing a large quantity of high-quality paphiopedilum helenae seedlings in a short time. The method has the advantages of high seed germination rate, rapid seedling emergence and high quality of seedlings; the paphiopedilum helenae seedlings can be applied to the supply market, ex-situ conservation and natural regression; the resource protection and the sustainable utilization of paphiopedilum helenae can be facilitated.

The invention discloses a method for seedling paris polyphylla Smith in high efficiency, and the method comprises the steps of seed selection, pretreatment, first preservation at a low temperature, first enzyme processing, second preservation at the low temperature, second enzyme processing, sand storage, sowing and seedling maintenance. According to the seedling method, the seedling emergence cycle of the paris polyphylla Smith is shortened to 220-270 days; the emergence rate is up to more than 85%; the seedling period is greatly shortened; and the emergence rate of the paris polyphylla Smith is raised. Therefore, a foundation for breeding the paris polyphylla Smith sprouts rapidly and producing in large scale is provided.

The invention discloses a method for cutting and propagating a chamelaucium plant. The method comprises the following steps: collecting a healthy plant without diseases and pests as a female parent material and scissoring newborn tender twigs above the half-lignifying position of the female parent material as cutting ears; dipping the basic parts of the cutting ears by 500-2000ppm of indole-3-butanoic acid solution and then carrying out bactericidal treatment on the cutting ears by a solution with 1500 times of gejunqing of 2.5% after drying in the air; and taking the cutting depth as 1 / 3-1 / 4of the length of the cutting ears, managing by a conventional technology after cutting, and carrying out field transplantation after the roots of the cutting ears grow out of the bottom of a seedlingdisk. By applying the method, the survival rate of the cutting and the propagation of the chamelaucium plant reaches more than 90%, and the propagation period is shortened to 35-40 days. The obtainedchamelaucium seeds and seedlings have superior quality and long service life, so that the popularized planting of the chamelaucium plant becomes possible. The invention has simple and convenient method, low cost, high survival rate of the seeds and the seedlings and favorable market application prospect.

The present invention relates to a sterile sowing and tissue culture method of epidendrum radians. The invention adopts artificial nutrient liquor and utilizes sterile sowing method to oblain lots of test-tube seedlings, and utilizes the stem apex or stipes of sterile seedling-plant to make multiplication of clustered bud, then utilizes rootage and culture to form whole plant.

The invention discloses a method for crossbreeding and aseptic sowing of renanthera coccinea, comprising the following steps of: carrying out hybridization by selecting orchid with obvious characters and high visual value as a hybrid female parent and renanthera coccinea as a male parent; carrying out aseptic sowing on a seed germination medium on the fruit obtained by successful pollination when being basically ripened but not splitted; after germinating, transferring the seeds to a healthy seedling medium; and growing the formed seedling in a test tube out of a bottle after being hardened under the illumination of natural light for 10 to 20 days, wherein the transplanting survival rate of seedling formation can reach 90% or more. The method has the characteristic of high hybrid success rate, and is particularly useful during taking orchid with obvious characters and high visual value as a hybrid male parent which is different from a renanthera coccinea parent in flowering phase. The unique medium and cultivation method are employed by the invention so that the hybrid seeds of renanthera coccinea have high germination, fast seedling formation and good seedling quality. The method has the advantages of strong operation, effective breeding, valuable application and the like.

The invention discloses a dendrobium candidum tissue culture plant hormone control method. The method comprises the following steps of: performing culture for 8 hours every day under the conditions that the humidity is 70 to 80 percent, the temperature is 23 to 28 DEG C and the illumination is 1,800 to 2,200LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 29 DEG C and the illumination is 1,000 to 1,400LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 25 DEG C and the illumination is 400 to 800LX; and after the dendrobium candidum is treated by the conventional sterilization program, sequentially culturing the dendrobium candidum through the following three stages: 1) callus culture; 2) adventitious bud induced culture; and 3) rooted seedling culture. The method has the advantages of greatly improving the dendrobium candidum tissue culture efficiency, optimizing the quality of the seedlings, shortening the culture period and providing a prerequisite for industrialized development of the dendrobium candidum.

The invention discloses a triarrhena lutarioriparia clonal seedling method, and the method comprises the steps as follows: (1) making pool, (2) watering, (3) covering the film, (4) root cutting and naturally growing. The triarrhena lutarioriparia clonal seedling method is convenient and easy in operation and low in cost for outdoor seeding, the emergence rate of the triarrhena lutarioriparia at the coastal saline-alkali stem section can be obviously raised and the excellent sprout can be obtained, the method gives the foundation for obtaining triarrhena lutarioriparia and artificial reproduction and solves the difficult outdoor seeding problem for seaside marginal soil.

The invention discloses a method for novel variety culture and rapid tissue culture propagation of paphiopedilum and relates to the technical field of breeding and tissue culture of paphiopedilum. Themethod for novel variety culture and rapid tissue culture propagation of paphiopedilum comprises the following steps: performing artificial hybridization pollination, performing sterile seed sowing and seed germination, strengthening seedlings, promoting rooting, and transplanting test tube seedlings. The method is good in operability, high in application value, high in hybridization success rate, and very effective for excellent cross parents of paphiopedilum in flowering asynchronism; in the rapid tissue culture propagation process, a unique seed germination culture medium and a seedling strengthening rooting culture medium are used, so that the germination rate of paphiopedilum hybridization seeds is increased, seedlings can grow rapidly, and the seedlings are good in quality. The method for novel variety culture and rapid tissue culture propagation of paphiopedilum, which is disclosed by the invention, has the characteristics of being good in operability, high in application value, high in hybridization success rate, and the like, the problems that conventional novel variety culture and rapid tissue culture propagation process of paphiopedilum is low in germination rate, hardin seedling growth, long in propagation cycle, low in propagation coefficient, large in propagation difficulty, and the like, are solved, an effective way is provided for production of paphiopedilum seedlings, and industrial planting can be achieved.

The invention provides a paphiopedilum delenatii seed non-symbiotic germination method, and belongs to the technical field of plant tissue culture. According to the paphiopedilum delenatii seed non-symbiotic germination method, paphiopedilum delenatii seeds are used as explants, and the germination and growth rates of the seeds are improved by performing sowing, differentiation, seedling strengthening and rooting culture on a specific culture medium under a light environment condition; and the germination rate of the seeds is high, the seedling emergence speed is high, the seedling quality is good, and a large number of high-quality paphiopedilum delenatii seedlings are produced within a short time. Experimental results of the embodiment show that the germination rate can reach 47.7% when the paphiopedilum delenatii seeds are cultured for 60 days by the method, the paphiopedilum delenatii seeds can be differentiated into plantlets with the height of about 1.0 cm after 40 days, the plantlets can grow into complete plants with the plant height of 3-4 cm and developed root systems after 60 days, and the transplanting survival rate can reach 100%.

The process of raising blueberry seedling includes the following steps: gathering mature blueberry seed, spreading one layer of blueberry seed in a culture dish, soaking the seed with water, lighting at 1000-2000 LX for 8-14 hr / day at 20-30 deg.c to culture while maintaining the humidity for 10-20 days, mixing the seed with soil, seeding in seed bed, covering seed with fine soil in the thickness of 2-5 times seed thickness, and spraying water to maintain soil humidity. The process meets the special lighting requirement for blueberry seed to germinate in advance and has high seedling emergence rate and high seedling quality.

The invention relates to a method for rapid propagation of high-quality seedlings of oncidium and the used culture medium. Oncidium, also known as Dancing Girl Orchid, King Phalaenopsis, Tubal Orchid, etc., is one of the largest genus in the orchidaceae. Oncidium has bright colors, large numbers of flowers, and large changes in flower shapes. Some varieties also have a delicate fragrance. It is one of the most widely cultivated and popular orchid species in the world. Many oncidium varieties have been introduced in mainland China in recent years, but the production of seedlings mainly depends on ramets, the propagation speed is slow, and the diseases of the female parent are also easy to infect the seedlings. The invention provides a variety of oncidium original species and hybrid tissue culture propagation methods and the used unique culture medium. The produced oncidium seedlings have the advantages of high genetic stability, fast emergence, strong seedlings, good seedling quality, strong resistance, and good growth. High-quality oncidium seedlings have been produced, and the market is very promising.

The invention discloses a seedling planting device for sapling planting and a using method thereof. The device comprises a vehicle body, a conveying mechanism is connected into the vehicle body and used for conveying saplings, the vehicle body is rotationally connected with a hole digging wheel through a rotating shaft, and the rotating shaft is connected with a rotating motor; a cavity is formed in the hole digging wheel, a plurality of earth cutting openings are further formed in the hole digging wheel, the earth cutting openings are communicated with the cavity, a plurality of earth outlets are further formed in one side of the hole digging wheel, and the earth outlets are formed in one side of the earth cutting openings; the vehicle body is further connected with an inclined conveying groove, the conveying groove is formed in one side of the hole digging wheel and makes contact with the lower end of the hole digging wheel, the conveying groove communicates with a soil covering groove, and the soil covering groove is connected with the vehicle body and is formed in the left side of the hole digging wheel; the vehicle body is further connected with an electric push rod, the electric push rod is connected with an electric clamping hand, and the electric clamping hand is arranged between the hole digging wheel and the soil covering groove. According to the device, equal-distance seedling planting work can be achieved, integrated operation of pit digging, seedling planting and soil covering is achieved, the seedling planting efficiency and quality are effectively improved, and repeated labor is not needed.

The invention discloses a soilless culture rapid seedling culturing method and system inside a brassicaceous vegetable greenhouse. The soilless culture rapid seedling culturing method and system inside the brassicaceous vegetable greenhouse is used for culturing seedlings inside a seedling culturing house. The method comprises the following steps that disinfected peat soil and perlite are mixed according to the ratio of 4-5:1 to be uniform to be used as a matrix, the matrix is laid on a seedling culturing plate, and 0.5-0.8 cm deep holes are formed in the seedling culturing plate; seeds are planted into the holes, then, the matrix is laid flatly, and the seedling culturing plate is placed on a seedling culturing tray; a nutrient solution is pumped to the bottom of the seedling culturing tray, and the nutrient solution permeates upwards through the small holes in the bottom of the seedling culturing plate; the environment temperature is kept to range from 16 DEG C to 25 DEG C, and the humidity is kept to range from 65% to 100%; after seeds begin to break ground, LED light sources with the ratio of white light sources to red light sources being 1:1-9:1 are adopted for illumination. By adopting the technical scheme, no pesticide needs to be adopted, the seedling growth speed is high, seedling leaves are thick and large, stems are thick and solid, harvesting is performed early, the yield is high, and automatic control can be achieved.

The present invention provides a test-tube breeding method for Nothodoritis germchit, selecting a Nothodoritis stock plant growing vigorously to implement an artificial pollination when flowering, using a fruitage without dehiscence growing until basic ripeness after the pollination as an explant, seeding sterilely or proliferating and differentiation culturing an original bulbodium, good seedling culturing and transplanting and the like. Implementing a sterile seeding and a following generation proliferation to the original bulbodium by employing a confecting-specially culture medium to obtain a young seedling, and culturing the young seedling in a confecting-specially culture medium and transplating. The method of the invention has a high seed sprouting rate, a rapid seedling formation, a good seedling quality, a low cost and the like, it is capable of obtaining a great amount of test-tube plantlets in a short time, and a transplantation survival of the seedling is more than 90%. The invention provides an effective approach for producing the Nothodoritis germchit.

The invention provides a hybridization breeding and rapid propagation method of anthurium. The hybridization breeding and rapid propagation method sequentially comprises the following six steps: material selection, manual pollination, sterile seed sowing and culture, propagation culture, seedling strengthening and rooting culture and test-tube plantlet transplanting. With the adoption of the method provided by the invention, the hybridization breeding success rate of the anthurium can be improved, certain asynchronous flowering period problems of hybridized combination are solved and high-quality germchits are rapidly cultured; and the hybridization breeding and rapid propagation method has the advantages of less investment, high yield and like, and is applied to large-scale production and protection of germplasm resources of the anthurium.

The invention provides a grafting propagation method of hybrid citrus. The grafting propagation method of hybrid citrus has the beneficial effects that the factory production effect is realized, the grafting time is long, the growth speed is quick, the growth is vigorous, and the quality of seeds and seedlings are high; the method is simple, convenient and practical, and the survival rate of the seeds and seedlings can reach 95% or above; compared with an exposed cultivation grafting method, the survival rate can be increased by 10% or above.

The present invention provides artificial blueberry seedling raising process, and features that the blueberry seed after being sterilized is inoculated to MS culture medium under aseptic condition for culturing under tissue culture condition. The present invention meets the specific requirement of blueberry seed during germination, and has simple operation, short seedling raising period, high germination rate and other advantages. The present invention makes it possible to obtain great amount of blueberry seedlings in short term by means of industrial production.

The invention relates to a method for cultivating the novel variety of tetraploid dendrobium by use of the seed protocorm (PLBs). After the artificial pollination, the immature dendrobium seed in the development stage is carried out non-symbiotic sproution cultivation for 60 days in the 1 / 2MS liquid culture medium, which is then transferred into the 1 / 2MS liquid culture medium containing 0.02 percent Oryzalin for process and then transferred into the 1 / 2MS solid culture medium for continuous cultivation; after that, till the dendrobium seedling is shaped, the dendrobium is not planted, transplanted and managed by a conventional method until the dendrobium blooms. The invention overcomes the defect of generally low mutagenesis frequency of the polyploid and has better appreciation; moreover, the method is easy and practical, which increases the mutagenesis frequency (by over 60 percent) of the polyploidy in a large scale and provides enough polyploidy (over hundreds of plants) obtainedone time to be used for the seed selection with high application value. Besides, the invention is applicable to the large-scale production with the advantages of small investment and high output.

The invention discloses a tissue culturing and rapid propagation method of dendrobiumtosaense makino seedlings. The method comprises the steps of: selection and treatment of raw materials, sterilization of explants, induction and proliferation of adventitious shoots, rooting and hardening-seedling culture and the like. In the step of induction and proliferation of the adventitious shoots, cluster shoots are cut into single shoot, and then the single shoot is inoculated on a rooting and hardening-seedling culture medium for culturing to obtain a complete plant; the step of the induction and proliferation of the adventitious shoots is as follows: based on improved MS ( Murashige and Skoog), adding 3.0 to 5.0 milligrams / liters of 6-benzyladenine, 3.0 to 5.0 milligrams / liters of kinetin, 1.0 to 2.0 milligrams / liters of naphthaleneacetic acid and 10 to 20 percent of coconut juice; the rooting and hardening-seedling culture medium is the improved MS culture medium, based on the improved MS, 0.5 to 1.0 milligrams / liters of naphthaleneacetic acid, 0.5 to 1.0 milligrams / liters of indolebutyric acid, 50 to 150 grams / liters of banana juice and 0.5 to 1.0 grams / liters of activated carbon are added, and the improved MS culture medium is 1 / 2 of MS. According to the method disclosed by the invention, tissue culturing and propagation on raw green shoots can be carried out in a whole year, and the method has the characteristics of quick propagation speed, excellent quality of seedlings, uniform offspring characteristics and the like; moreover, the tissue culturing and propagation can be completed by only needing simple tissue culturing equipment.

The present invention relates to a cultivation method of Chinese herbs, and especially relates to an organic plantation method of Guangxi Yao nationality classic medicine Herba Sarcandrae. The organic plantation method comprises the steps of seedling selection, soil preparation, fertilizer application and field management; the seedling selection comprises the steps of selecting robust seedlings cultivated by the Chinese patent 'tissue culture rapid propagation method of Herba Sarcandrae medicine seedlings', and loading the seedlings into substrate soil prepared by mixture of peat soil and river sand with the weight ratio of 1:1 for preparation; and the soil preparation and fertilizer application comprise the steps of selecting a piece of field good in drainage, appropriately fertilizing compost in the field before cultivation, dosage of the compost being 1000-1500 kilograms per mu, then cultivating the field by utilization of a hydraulic deep rotary tillage ridge smashing machine, and finally ditching and planting the prepared seedlings. The Herba Sarcandrae obtained by the organic plantation method is prevented from being polluted by pesticide, hormone and chemical medicines, and is natural, safe and reliable, and quality of the Herba Sarcandrae is a notch above that of normally planted Herba Sarcandrae, thereby largely increasing economic benefit.

The invention discloses a tissue culture reproduction method of Chinese evergreen and aims to provide a tissue culture reproduction method of the Chinese evergreen with high reproduction speed, mass test seedling obtainment in a short term, little damage to a mother plant and the maintenance of mother plant fine traits. The method comprises the following steps: selecting young stems of eugenic Chinese evergreen as explants, disinfecting the explants, carrying out the adventitious-bud induction of an induction culture medium and coupling adventitious buds into a reproduction culture medium forsuccessive transfer reproduction; cutting and inoculating the buds to a rooting culture medium for rooting culture while the buds grow to the height of 3cm to 5cm; training the seedlings under natural light for 7 days to 10 days when the test seedlings grow to the height of 6cm to 7cm; and taking the test seedlings out, washing the rooting culture medium, planting in a volumetric mixture medium comprising sand, perlite, peat soil, and the like and obtaining planting seedlings after conventional culture. The invention can provide markets with mass Chinese evergreen test seedlings.