76results about How to "Seedlings of good quality" patented technology

The invention provides a multi-flower paphiopedilum test-tube seedling cultivation method, belonging to the technical field of plant growing. The method is characterized by comprising the following steps: 1) pod acquisition; 2) aseptic seeding; 3) multiplication subculture; 4) root and strong seedling culture; and 5) test-tube seedling transplantation. The multi-flower paphiopedilum test-tube seedling cultivation method has the characteristics of high germination rate, short seedling emergence cycle, robust seedlings, developed root systems, good seedling quality, low production cost and the like, can be used for large-scale industrialized production of paphiopedilum and has a good application prospect.

Disclosed is the high quality germchit tissure culturing and rapid breeding method of Dendrobium sp., wherein a large amount of test tube seedlings with stabilized performance are obtained through fine breed tissue culture by adopting the path of clumping buds. The produced Dendrobium sp. has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and fine growth status.

The invention discloses a ficus tikoua rapid propagation method. According to the method, ficus tikoua terminal buds or axillary buds with stem sections are cultured in an initial medium, such that aseptic seedlings are obtained; clustered buds are induced on a proliferation medium; and rooting is carried out on a rooting medium. When ficus tikoua tissue culture is carried out with the set of technical methods provided by the invention, the period is short, propagation coefficient is high, cost is low, and high factory production capacity is provided. When propagation is carried out with the method provided by the invention, ficus tikoua can be effectively protected, developed, and utilized.

The present invention relates to the sterilized seeding and tissue cultivation technology of Vanda as one of four main kinds of ornamental orchid. The present invention obtains great amount of test seedling of Vanda through sterilized seeding in artificial culture medium, and forms complete plant via proliferation and rooting culture of stem tip or stem node of abacterial seedling. The present invention has practical operation and high application value.

A method for tissue culturing and fast breeding the breed sprout of sarcandra Folium et Ramulus crude drugs, relating to the seedling method of tissue culturing and fast breeding the sarcandra Folium et Ramulus crude drugs through the biological tissue culture and asexual propagation technique, comprising the following five steps: choosing the sarcandra Folium et Ramulus with basistipes or apical as explant for surface sterilization; first generation culturing for inducing the clump budding; breeding culture; root growing culturing; refining sprouts and replanting. The invention is characterized by the fast emergence of seedlings, high stability, strong sprouts, high quality of seed sprout, strong resistant, good growing condition and strong practicability, etc; which solves the sarcandra Folium et Ramulus crude drugs seed supply for the planting, and provides safeguard for the GAP stander management for the sarcandra Folium et Ramulus crude drugs planting, and provides quality guarantee for the medical production which applying the sarcandra Folium et Ramulus crude drugs as raw material.

The invention discloses a method for seedling paris polyphylla Smith in high efficiency, and the method comprises the steps of seed selection, pretreatment, first preservation at a low temperature, first enzyme processing, second preservation at the low temperature, second enzyme processing, sand storage, sowing and seedling maintenance. According to the seedling method, the seedling emergence cycle of the paris polyphylla Smith is shortened to 220-270 days; the emergence rate is up to more than 85%; the seedling period is greatly shortened; and the emergence rate of the paris polyphylla Smith is raised. Therefore, a foundation for breeding the paris polyphylla Smith sprouts rapidly and producing in large scale is provided.

The invention discloses a method for cutting and propagating a chamelaucium plant. The method comprises the following steps: collecting a healthy plant without diseases and pests as a female parent material and scissoring newborn tender twigs above the half-lignifying position of the female parent material as cutting ears; dipping the basic parts of the cutting ears by 500-2000ppm of indole-3-butanoic acid solution and then carrying out bactericidal treatment on the cutting ears by a solution with 1500 times of gejunqing of 2.5% after drying in the air; and taking the cutting depth as 1/3-1/4of the length of the cutting ears, managing by a conventional technology after cutting, and carrying out field transplantation after the roots of the cutting ears grow out of the bottom of a seedlingdisk. By applying the method, the survival rate of the cutting and the propagation of the chamelaucium plant reaches more than 90%, and the propagation period is shortened to 35-40 days. The obtainedchamelaucium seeds and seedlings have superior quality and long service life, so that the popularized planting of the chamelaucium plant becomes possible. The invention has simple and convenient method, low cost, high survival rate of the seeds and the seedlings and favorable market application prospect.

The present invention relates to a sterile sowing and tissue culture method of epidendrum radians. The invention adopts artificial nutrient liquor and utilizes sterile sowing method to oblain lots of test-tube seedlings, and utilizes the stem apex or stipes of sterile seedling-plant to make multiplication of clustered bud, then utilizes rootage and culture to form whole plant.

The invention discloses a dendrobium candidum tissue culture plant hormone control method. The method comprises the following steps of: performing culture for 8 hours every day under the conditions that the humidity is 70 to 80 percent, the temperature is 23 to 28 DEG C and the illumination is 1,800 to 2,200LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 29 DEG C and the illumination is 1,000 to 1,400LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 25 DEG C and the illumination is 400 to 800LX; and after the dendrobium candidum is treated by the conventional sterilization program, sequentially culturing the dendrobium candidum through the following three stages: 1) callus culture; 2) adventitious bud induced culture; and 3) rooted seedling culture. The method has the advantages of greatly improving the dendrobium candidum tissue culture efficiency, optimizing the quality of the seedlings, shortening the culture period and providing a prerequisite for industrialized development of the dendrobium candidum.

The invention discloses a triarrhena lutarioriparia clonal seedling method, and the method comprises the steps as follows: (1) making pool, (2) watering, (3) covering the film, (4) root cutting and naturally growing. The triarrhena lutarioriparia clonal seedling method is convenient and easy in operation and low in cost for outdoor seeding, the emergence rate of the triarrhena lutarioriparia at the coastal saline-alkali stem section can be obviously raised and the excellent sprout can be obtained, the method gives the foundation for obtaining triarrhena lutarioriparia and artificial reproduction and solves the difficult outdoor seeding problem for seaside marginal soil.

The invention provides a paphiopedilum delenatii seed non-symbiotic germination method, and belongs to the technical field of plant tissue culture. According to the paphiopedilum delenatii seed non-symbiotic germination method, paphiopedilum delenatii seeds are used as explants, and the germination and growth rates of the seeds are improved by performing sowing, differentiation, seedling strengthening and rooting culture on a specific culture medium under a light environment condition; and the germination rate of the seeds is high, the seedling emergence speed is high, the seedling quality is good, and a large number of high-quality paphiopedilum delenatii seedlings are produced within a short time. Experimental results of the embodiment show that the germination rate can reach 47.7% when the paphiopedilum delenatii seeds are cultured for 60 days by the method, the paphiopedilum delenatii seeds can be differentiated into plantlets with the height of about 1.0 cm after 40 days, the plantlets can grow into complete plants with the plant height of 3-4 cm and developed root systems after 60 days, and the transplanting survival rate can reach 100%.

The process of raising blueberry seedling includes the following steps: gathering mature blueberry seed, spreading one layer of blueberry seed in a culture dish, soaking the seed with water, lighting at 1000-2000 LX for 8-14 hr/day at 20-30 deg.c to culture while maintaining the humidity for 10-20 days, mixing the seed with soil, seeding in seed bed, covering seed with fine soil in the thickness of 2-5 times seed thickness, and spraying water to maintain soil humidity. The process meets the special lighting requirement for blueberry seed to germinate in advance and has high seedling emergence rate and high seedling quality.

The invention discloses a seedling planting device for sapling planting and a using method thereof. The device comprises a vehicle body, a conveying mechanism is connected into the vehicle body and used for conveying saplings, the vehicle body is rotationally connected with a hole digging wheel through a rotating shaft, and the rotating shaft is connected with a rotating motor; a cavity is formed in the hole digging wheel, a plurality of earth cutting openings are further formed in the hole digging wheel, the earth cutting openings are communicated with the cavity, a plurality of earth outlets are further formed in one side of the hole digging wheel, and the earth outlets are formed in one side of the earth cutting openings; the vehicle body is further connected with an inclined conveying groove, the conveying groove is formed in one side of the hole digging wheel and makes contact with the lower end of the hole digging wheel, the conveying groove communicates with a soil covering groove, and the soil covering groove is connected with the vehicle body and is formed in the left side of the hole digging wheel; the vehicle body is further connected with an electric push rod, the electric push rod is connected with an electric clamping hand, and the electric clamping hand is arranged between the hole digging wheel and the soil covering groove. According to the device, equal-distance seedling planting work can be achieved, integrated operation of pit digging, seedling planting and soil covering is achieved, the seedling planting efficiency and quality are effectively improved, and repeated labor is not needed.

The invention discloses a soilless culture rapid seedling culturing method and system inside a brassicaceous vegetable greenhouse. The soilless culture rapid seedling culturing method and system inside the brassicaceous vegetable greenhouse is used for culturing seedlings inside a seedling culturing house. The method comprises the following steps that disinfected peat soil and perlite are mixed according to the ratio of 4-5:1 to be uniform to be used as a matrix, the matrix is laid on a seedling culturing plate, and 0.5-0.8 cm deep holes are formed in the seedling culturing plate; seeds are planted into the holes, then, the matrix is laid flatly, and the seedling culturing plate is placed on a seedling culturing tray; a nutrient solution is pumped to the bottom of the seedling culturing tray, and the nutrient solution permeates upwards through the small holes in the bottom of the seedling culturing plate; the environment temperature is kept to range from 16 DEG C to 25 DEG C, and the humidity is kept to range from 65% to 100%; after seeds begin to break ground, LED light sources with the ratio of white light sources to red light sources being 1:1-9:1 are adopted for illumination. By adopting the technical scheme, no pesticide needs to be adopted, the seedling growth speed is high, seedling leaves are thick and large, stems are thick and solid, harvesting is performed early, the yield is high, and automatic control can be achieved.

The invention provides a hybridization breeding and rapid propagation method of anthurium. The hybridization breeding and rapid propagation method sequentially comprises the following six steps: material selection, manual pollination, sterile seed sowing and culture, propagation culture, seedling strengthening and rooting culture and test-tube plantlet transplanting. With the adoption of the method provided by the invention, the hybridization breeding success rate of the anthurium can be improved, certain asynchronous flowering period problems of hybridized combination are solved and high-quality germchits are rapidly cultured; and the hybridization breeding and rapid propagation method has the advantages of less investment, high yield and like, and is applied to large-scale production and protection of germplasm resources of the anthurium.

The invention provides a grafting propagation method of hybrid citrus. The grafting propagation method of hybrid citrus has the beneficial effects that the factory production effect is realized, the grafting time is long, the growth speed is quick, the growth is vigorous, and the quality of seeds and seedlings are high; the method is simple, convenient and practical, and the survival rate of the seeds and seedlings can reach 95% or above; compared with an exposed cultivation grafting method, the survival rate can be increased by 10% or above.

The present invention provides artificial blueberry seedling raising process, and features that the blueberry seed after being sterilized is inoculated to MS culture medium under aseptic condition for culturing under tissue culture condition. The present invention meets the specific requirement of blueberry seed during germination, and has simple operation, short seedling raising period, high germination rate and other advantages. The present invention makes it possible to obtain great amount of blueberry seedlings in short term by means of industrial production.

The invention discloses a tissue culturing and rapid propagation method of dendrobiumtosaense makino seedlings. The method comprises the steps of: selection and treatment of raw materials, sterilization of explants, induction and proliferation of adventitious shoots, rooting and hardening-seedling culture and the like. In the step of induction and proliferation of the adventitious shoots, cluster shoots are cut into single shoot, and then the single shoot is inoculated on a rooting and hardening-seedling culture medium for culturing to obtain a complete plant; the step of the induction and proliferation of the adventitious shoots is as follows: based on improved MS ( Murashige and Skoog), adding 3.0 to 5.0 milligrams/liters of 6-benzyladenine, 3.0 to 5.0 milligrams/liters of kinetin, 1.0 to 2.0 milligrams/liters of naphthaleneacetic acid and 10 to 20 percent of coconut juice; the rooting and hardening-seedling culture medium is the improved MS culture medium, based on the improved MS, 0.5 to 1.0 milligrams/liters of naphthaleneacetic acid, 0.5 to 1.0 milligrams/liters of indolebutyric acid, 50 to 150 grams/liters of banana juice and 0.5 to 1.0 grams/liters of activated carbon are added, and the improved MS culture medium is 1/2 of MS. According to the method disclosed by the invention, tissue culturing and propagation on raw green shoots can be carried out in a whole year, and the method has the characteristics of quick propagation speed, excellent quality of seedlings, uniform offspring characteristics and the like; moreover, the tissue culturing and propagation can be completed by only needing simple tissue culturing equipment.