7 results about "Cymbidium goeringii" patented technology

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.

This invention discloses a rapid propagation method for the endangered orchid *Cymbidium goeringii* via tissue culture, belonging to the field of plant tissue culture technology. The method includes capsule sterilization and aseptic seed germination, protocorm proliferation, protocorm differentiation, rooting culture, and transplantation of tissue culture seedlings. Its key feature is that protocorm proliferation induction uses MS medium containing 0.05 mg / L–1.0 mg / L TDZ, and protocorm adventitious bud induction is continuously performed on the same medium without the need for transfer or medium change. This invention is the first to apply TDZ to the rapid propagation system of *Cymbidium goeringii*, achieving both proliferation and differentiation steps on the same medium, greatly simplifying the operation process and reducing costs. Protocorm proliferation is 5–6 times after 21 days of culture, and can reach more than 15 times after 60 days, with high rooting and transplant survival rates; the survival rate reaches 98% after 30 days of transplanting. This method is simple to operate, has a short cycle, a high propagation coefficient, and low cost, making it suitable for large-scale industrial seedling production and of great significance for the protection and sustainable utilization of *Cymbidium goeringii* germplasm resources.

This invention discloses a suitable cultivation substrate for the growth of *Cymbidium goeringii* and its application, belonging to the field of plant cultivation technology. The cultivation substrate is composed of a single type of cedar sawdust and is used for *Cymbidium goeringii* cultivation. Using the cedar sawdust cultivation substrate, after 6 months of transplanting, the number of *Cymbidium goeringii* seedlings reached 2.20±0.45 seedlings / plant, higher than that of sphagnum moss substrate (1.20±0.45 seedlings / plant), coconut fiber substrate (1.80±0.84 seedlings / plant), and other tested substrates. This indicates that cedar sawdust can effectively promote the germination of axillary buds in *Cymbidium goeringii*, which is beneficial for the rapid propagation of seedlings.

The application discloses orchid-flavor instant Pu'er ripe tea and a preparation method thereof. The application uses specific orchid and Pu'er ripe tea, optimized extraction parameters (ethanol concentration, solid-liquid ratio and extraction time) and selective extraction and retention of ingredients by a spray drying process, so that the product is enriched with specific terpenes, esters and other aroma substances (such as big root myrcene D and ethyl hexanoate) of Cymbidium goeringii and flavor ingredients (such as amino acids and soluble sugar) of Pu'er tea, and the proportions of the ingredients are coordinated. Technical advantages: the product prepared by the method has orchid fragrance, and has the functions of antibiosis and antioxidation. The product has the functions of the Pu'er tea, and has good solubility and quality stability. The product is obviously different from traditional instant Pu'er ripe tea, and the drinking value and market recognition of the product are improved.

The application discloses a kind of adjustable microclimate film cultivation device for Chinese orchid cultivation, the device is suitable for being arranged in greenhouse environment, including support frame, the support frame is fixedly connected on hardened ground, the support frame top horizontal laying is covered with film plate, the lateral film of support frame side vertical surface can be detachably connected, a plurality of cultivation holes are formed in the film plate, the cultivation hole is inserted with special flowerpot of Chinese orchid, the support frame below is natural soil layer, the support frame around is the hardened ground, the hardened ground has gradient, and the support frame is located at high place.The device has no energy consumption, low cost, solves the multiple needs of Chinese orchid "ventilation-moisture-flood prevention-heat preservation", and is suitable for efficient cultivation of cold-sensitive varieties such as cymbidium goeringii.