26 results about "Cymbidium goeringii" patented technology

The invention discloses a coprinus comatus strain for promoting seed germination of cremastra appendiculata and application of the coprinus comatus strain, the strain is separated and screened from root tissues of cremastra appendiculata, the strain is identified as coprinus comatus through ITS (internal transcribed spacer) sequencing, the Latin name is Coprinella micaceus, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO: 42051. The strain is inoculated into a liquid culture medium for shake culture, and mycelia are collected and mixed with sterile vermiculite and turfy soil to prepare a fungicide; the method comprises the following steps: laying a sterilized culture medium formed by mixing leaf mold, perlite and bark in a sterile culture container, spreading a fungicide, spreading the treated azalea seeds, covering the culture medium, and culturing by controlling the temperature, the illumination condition and the relative humidity. According to the method, the germination rate of the rhododendron simsii seeds can reach 60%-70%, the germination time is shortened to 15-20 days, the survival rate of seedlings is high, operation is easy and convenient, cost is low, the method is suitable for large-scale breeding and resource protection of the rhododendron simsii, and a new thought is provided for research on symbiotic germination of orchid plants.

The invention belongs to the technical field of orchid breeding, and particularly relates to a cypripedium Shanxi symbiotic protocorm cultivation method which comprises the following steps: preparing a nylon net film with the aperture of 30-80 microns into a seed bag with a bag-shaped structure, then filling cypripedium Shanxi seeds into the seed bag according to the quantity of 50-200 particles in each bag, sealing the bag opening, and then putting the cypripedium Shanxi symbiotic protocorm into the bag-shaped seed bag, thereby obtaining the cypripedium Shanxi symbiotic protocorm. The method comprises the following steps: burying the seeds in soil in a rhizosphere area of an adult plant in an original field of cypripedium Shanxi, and covering with an original habitat covering, so that the seeds are induced to form protocorms under a natural symbiotic condition. The method provided by the invention is simple and convenient to operate, the obtained cypripedium Shanxi symbiotic protocorm has natural symbiotic activity and excellent ecological adaptability, and efficient recovery of the cypripedium Shanxi symbiotic protocorm can be realized.

The invention discloses a Chinese orchid bud dormancy control gene CsABF2 as well as a coding sequence and application thereof, and belongs to the field of plant molecular biology. The invention finds that the expression of CsABF2 in Chinese cymbidium stems is the highest; in different flower development stages of cymbidium sinense, the expression trend of CsABF2 is that the expression quantity is reduced after S1 and is gradually increased after S2; in tissue parts of mature flowers of cymbidium sinense, CsABF2 has the highest expression in labial flaps. After the CsABF2 is subjected to heterologous expression in arabidopsis thaliana, the arabidopsis thaliana is found to have a phenotype of flowering delay. After CsABF2 is silenced in Chinese orchid, the phenotype of flowering in advance can appear. The Chinese orchid flowering regulation and control gene can be used for research of orchid flower differentiation molecular mechanism and improvement of plant flowering traits.

The invention provides a method for promoting seed germination of cremastra appendiculata, which comprises the following steps: selecting mature cremastra appendiculata capsules, and taking out seeds in the cremastra appendiculata capsules; uniformly mixing the seeds in the five rhododendron capsules with one crushed grain coprinus comatus cultivated species; a mixture of the seeds and the grain coprinus comatus cultivated species is put into a transparent adhesive bag, the humidity is controlled to be 60-80%, and a bag opening of the transparent adhesive bag is sealed; 2-5 air holes are evenly formed in each of the two sides of the transparent adhesive bag, and the hole diameter is 0.5-1.5 cm; and placing the transparent adhesive bag filled with the mixture in an environment with the illumination intensity of 0lx, the relative humidity outside the transparent adhesive bag of 60-80% and the temperature of 18-22 DEG C until protocorms are generated. According to the method, azalea seeds and crushed grain coprinus comatus cultivated species are evenly mixed according to the proportion and then put into a transparent plastic bag, full darkness, the constant temperature of 18-22 DEG C, the constant humidity environment of 60-80% and ventilation of the two sides of the plastic bag are controlled, the seed germination starting time and the time for forming protocorms with the diameter of 0.5 cm are short, operation is easy, cost is low, and the seed germination rate is high.

The invention provides a wild Tibet cymbidium tissue culture method which comprises the following steps: picking wild Tibet cymbidium capsules, cleaning with clear water, wiping with an ethanol solution, soaking with the ethanol solution, disinfecting with mercury bichloride, and soaking and washing with sterile water to obtain disinfected capsules; preparing capsule slices, placing the capsule slices above a sowing culture medium, knocking the edges of the capsule slices, dropwise adding sterile water, and shaking until seeds are dispersed on the surface of the culture medium and germinate and grow into protocorms; inoculating into a proliferation culture medium until rhizomes grow out, and carrying out differentiation culture. According to the method disclosed by the invention, the germination period of the wild cymbidium tigrinum seeds is shortened to less than 5 months, the germination rate is high, the rhizomes are thick, strong and full, the differentiation capability is strong, the speed is high, and the quality is high.

This invention belongs to the field of plant molecular biology technology, and specifically relates to a species of Cymbidium goeringii. CsCCD4 Genes and their role in regulating aroma. This invention provides a method for enhancing the synthesis of aroma compounds in orchids. CsCCD4 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was cloned to obtain the full-length gene sequence. Based on this, an overexpression vector was constructed and transient transformation of Phalaenopsis orchid petals and stable transformation of Nicotiana benthamiana were performed. The results showed that the content of the aroma compound β-ionone in the transiently transformed Phalaenopsis orchid petals and the stably transformed Nicotiana benthamiana petals was significantly increased compared to the control group (CK), indicating that... CsCCD4 It plays an important role in enhancing the fragrance compounds of orchids. As an important component in the synthesis of fragrance compounds in orchids... CsCCD4 Genes can be used in orchid genetic engineering to improve ornamental traits and create genetic varieties.

The invention discloses a staged seed and fungus symbiotic seedling raising method for cremastra appendiculata, and belongs to the technical field of planting of traditional Chinese medicinal materials. The method comprises the following steps: (1) indoor bacteria mixing and germination: mixing seeds of cremastra appendiculata and coprinus comatus germination bacteria cultivated species in a culture medium with a specific ratio, and culturing for 90-120 days indoors at 22 + / -2 DEG C until the seeds germinate and develop into protocorms (dragon eggs) with roots and stems; wood and strains are placed after outdoor land selection and preparation, protocorms in the differentiation period and a culture medium are broadcast on a fungus bed, humus soil of 2-3 cm covers the fungus bed, and a small arched shed is built for rain sheltering management till seedlings are formed. According to the method disclosed by the invention, indoor germination and outdoor planting are combined, so that the germination rate and the seedling rate of the seeds of the cremastra appendiculata are remarkably improved, and a reliable scheme is provided for large-scale artificial planting of the cremastra appendiculata.

The invention provides a planting method for increasing the number of Yunnan pleione bulbocodioides seedlings, and relates to the technical field of south pleione bulbocodioides seedling breeding. The method comprises the following steps that Yunnan pleione bulbocodioides pseudobulbs are provided; planting and field management; when the pseudobulb of the pleione bulbocodioides in Yunnan province begins to have terminal buds, auxin is sprayed; earthing up the periphery of the terminal bud of the pseudobulb of the Pleione bulbocodioides Yunnan; performing water management after the pseudobulb of the pleione bulbocodioides in Yunnan province grows a terminal bud; collecting terminal buds, and culturing the terminal buds again to obtain Yunnan pleione bulbocodioides seedlings. By means of the pseudobulb terminal bud proliferation mode, the number of Yunnan pleione bulbocodioides seedlings can be increased, and compared with a pseudobulb lateral bud proliferation mode, the number of Yunnan pleione bulbocodioides seedlings is increased by 4-5 times.

The present invention belongs to the field of plant cell engineering, and specifically relates to a spring orchid rhizome tissue culture medium and a tissue culture method containing animal-derived additives and anti-browning agents. The spring orchid rhizome tissue culture medium includes a proliferation culture medium, and the spring orchid tissue culture medium containing an anti-browning agent includes a differentiation culture medium. The proliferation culture medium is a basic proliferation culture medium added with animal-derived additives; the animal-derived additives include clams, preserved egg yolks, shrimps and squids. The differentiation culture medium is a basic differentiation culture medium added with an anti-browning agent; the anti-browning agent includes dithiothreitol, cysteine, glutathione, polyvinyl pyrrolidone, citric acid and succinic acid. This technical solution overcomes the technical problems of low reproduction coefficient, high browning rate and long culture cycle of the existing spring orchid tissue culture system. This solution can not only fill the gap in the study of factors affecting the growth of spring orchids, but also directly guide tissue culture and rapid propagation, provide a reference for factory seedling cultivation, and has important theoretical significance and practical value.

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

This invention discloses a gene for controlling the dormancy of Cymbidium orchid buds. CsABF2 Its coding sequence and applications belong to the field of plant molecular biology. This invention discovers that... CsABF2 The highest expression was observed in the stems of Cymbidium goeringii; it was also observed at different flower development stages in Cymbidium goeringii. CsABF2 The expression trend was that the expression level decreased after S1 and gradually increased after S2; in the tissue parts of mature flowers of Cymbidium goeringii, CsABF2 It is expressed most highly in the lip. Heterologous expression in Arabidopsis thaliana. CsABF2 Later, it was discovered that Arabidopsis thaliana exhibited a delayed flowering phenotype. (Silent in Cymbidium orchids) CsABF2 CsABF2 Subsequently, an earlier flowering phenotype may appear. The flowering regulatory gene of Cymbidium goeringii of this invention can be used to study the molecular mechanisms of flower differentiation in orchids and to improve the flowering traits of plants.

This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.

This invention discloses a rapid propagation method for the endangered orchid *Cymbidium goeringii* via tissue culture, belonging to the field of plant tissue culture technology. The method includes capsule sterilization and aseptic seed germination, protocorm proliferation, protocorm differentiation, rooting culture, and transplantation of tissue culture seedlings. Its key feature is that protocorm proliferation induction uses MS medium containing 0.05 mg / L–1.0 mg / L TDZ, and protocorm adventitious bud induction is continuously performed on the same medium without the need for transfer or medium change. This invention is the first to apply TDZ to the rapid propagation system of *Cymbidium goeringii*, achieving both proliferation and differentiation steps on the same medium, greatly simplifying the operation process and reducing costs. Protocorm proliferation is 5–6 times after 21 days of culture, and can reach more than 15 times after 60 days, with high rooting and transplant survival rates; the survival rate reaches 98% after 30 days of transplanting. This method is simple to operate, has a short cycle, a high propagation coefficient, and low cost, making it suitable for large-scale industrial seedling production and of great significance for the protection and sustainable utilization of *Cymbidium goeringii* germplasm resources.

This invention discloses a suitable cultivation substrate for the growth of *Cymbidium goeringii* and its application, belonging to the field of plant cultivation technology. The cultivation substrate is composed of a single type of cedar sawdust and is used for *Cymbidium goeringii* cultivation. Using the cedar sawdust cultivation substrate, after 6 months of transplanting, the number of *Cymbidium goeringii* seedlings reached 2.20±0.45 seedlings / plant, higher than that of sphagnum moss substrate (1.20±0.45 seedlings / plant), coconut fiber substrate (1.80±0.84 seedlings / plant), and other tested substrates. This indicates that cedar sawdust can effectively promote the germination of axillary buds in *Cymbidium goeringii*, which is beneficial for the rapid propagation of seedlings.

The application belongs to the technical field of Chinese medicinal material seedling breeding, and discloses a method for one-step seedling of Cymbidium goeringii tissue culture seedlings, which uses seeds as explants, carries out Cymbidium goeringii plant tissue culture induction seedling through 1 / 2MS culture medium, hormones NAA, 6-BA, ZT, sucrose, fresh milk, agar and active carbon and other related substances after disinfection and sterilization. The application can directly germinate from seeds, induce value-added differentiation and root seedling. The one-step seedling method reduces the processes of value-added differentiation and induction rooting of tissue culture seedlings, saves time cost and transfer cost, uses seeds as explants, and the roots of the one-step seedling are 4-5 cm long and the leaf buds are 10-15 cm long after 8-9 months of culture. The application solves the problems of long time consumption, high cost and complicated multi-stage transfer process in the existing plant tissue culture. The one-step seedling method for Cymbidium goeringii tissue culture seedlings adopts a simplified culture process, effectively shortens the culture period, reduces the cost, and enhances the consistency and health of plant growth.

The present invention discloses the coding sequence of the Cymbidium flowering regulation gene CsFTL1 and its application. The Cymbidium flowering regulation protein CsFTL is characterized in that its amino acid sequence is as shown in SEQ ID NO: 2. The present invention isolated the key flower development gene FTL1 from the flower bud cDNA of the Cymbidium sinense variety 'Xiaoxiang' and named it CsFTL1. Temporal and spatial expression pattern analysis found that the expression of CsFTL1 was the highest in the flowers of Cymbidium sinense; among different flower development stages of Cymbidium sinense, the expression trend of CsFTL1 was that the mRNA accumulation increased rapidly at the stage of flower bud differentiation and then gradually decreased. After heterologously expressing CsFTL1 in Arabidopsis thaliana, it was found that Arabidopsis thaliana showed the phenotype of early flowering. The Cymbidium flowering regulation gene of the present invention can be used for the study of the molecular mechanism of flower organ development in Orchidaceae plants and the improvement of plant flowering traits.

The application discloses orchid-flavor instant Pu'er ripe tea and a preparation method thereof. The application uses specific orchid and Pu'er ripe tea, optimized extraction parameters (ethanol concentration, solid-liquid ratio and extraction time) and selective extraction and retention of ingredients by a spray drying process, so that the product is enriched with specific terpenes, esters and other aroma substances (such as big root myrcene D and ethyl hexanoate) of Cymbidium goeringii and flavor ingredients (such as amino acids and soluble sugar) of Pu'er tea, and the proportions of the ingredients are coordinated. Technical advantages: the product prepared by the method has orchid fragrance, and has the functions of antibiosis and antioxidation. The product has the functions of the Pu'er tea, and has good solubility and quality stability. The product is obviously different from traditional instant Pu'er ripe tea, and the drinking value and market recognition of the product are improved.

The invention discloses a method for efficiently raising seedlings of cremastra appendiculata by using a mixture of corncobs and wood as a matrix, and belongs to the technical field of seedling raising of cremastra appendiculata. According to the method, through reasonable matrix matching and scientific seedling raising steps, the advantages of corncobs and wood are fully exerted, the seedling emergence time of the mixed breeding rhododendron simsii seeds is 40-50 days and is 5-20 days earlier, the current-year differentiation emergence rate of protocorms is 80% or above, the emergence rate is increased by 20% or above, seedlings grow robustly, remarkable economic benefits and social benefits are achieved, and the method is suitable for large-scale popularization and application. And the method is suitable for popularization and application in seedling production of the rhododendron.

The utility model discloses a cymbidium hookerianum cultivation device suitable for peasant houses, and belongs to the technical field of plant cultivation, the cymbidium hookerianum cultivation device comprises an adjustable supporting frame made of metal profiles, the adjustable supporting frame is provided with a plurality of multi-layer cultivation groove placing seat plates, and the multi-layer cultivation groove placing seat plates are provided with anti-corrosion wood cultivation grooves. Drainage holes are formed in the bottom of the anti-corrosion wood cultivation groove, ventilation holes are formed in the four side walls, and a sandy soil and cultivation medium mixed layer is laid in the groove. The device further comprises a water liquid spraying assembly used for irrigation and an atomizing spray head assembly used for humidity adjustment, a foldable drainage plate used for guiding residual liquid to be drained outwards is arranged below each layer of anti-corrosion wood cultivation groove, and a foldable rain-shading and sun-shading dual-purpose plate is arranged above the anti-corrosion wood cultivation groove on the topmost layer of the anti-corrosion wood cultivation groove on the topmost layer of the anti-corrosion wood cultivation groove on the topmost layer of the anti-corrosion wood cultivation groove. And a drawable base is arranged at the bottom of the adjustable supporting frame. The utility model has the advantages of high space utilization rate, convenient manual operation and low maintenance cost.

This invention discloses a gene for regulating the growth of Cymbidium goeringii. CsARF4 Its coding sequence and applications belong to the field of plant molecular biology. This invention's analysis revealed that... CsARF4 The highest expression was observed in the roots of Cymbidium goeringii; it was also observed at different flower development stages in Cymbidium goeringii. CsARF4 The expression trend is a gradual decrease; in the tissue parts of mature Cymbidium flowers, CsARF4 The expression is highest in the petals. This invention, through genetic engineering technology, discovered that the aforementioned gene regulating the growth and development of *Cymbidium goeringii* is overexpressed in *Cymbidium sinense*, altering plant growth traits, resulting in increased rhizomes and rapid growth. Therefore, this gene and its encoded protein can be used to study growth and development regulation pathways such as rhizome proliferation, increased budding rate, and promotion of rapid plant growth, as well as for improving growth traits.

This invention relates to the field of molecular breeding, and in particular to a KASP marker combination for identifying Cymbidium goeringii varieties with multiple perianth segments and unique flowers, its application, primers, and kits. The KASP markers include one or more of KASP1, KASP2, and KASP3. The three KASP markers designed and developed in this invention can be used for the selection and breeding of Cymbidium goeringii germplasm and hybrid offspring with multiple perianth segments. They can accurately distinguish between Cymbidium goeringii varieties with more than six perianth segments and normal Cymbidium goeringii varieties. This molecular marker combination can play a role in large-scale early screening of germplasm, providing effective assistance for molecular breeding of Cymbidium goeringii flower types.

The invention belongs to the technical field of microorganisms, and particularly relates to a CNOCC V.coerule-41 strain and application thereof. According to the invention, a CNOCC V.coerulea-41 strain is obtained through separation and screening, and the preservation number of the CNOCC V.coerulea-41 strain is GDMCC No: 67766. The strain provided by the invention has a stable growth promoting effect on various orchid plants, the strain is inoculated into pre-cultured seedlings or explants of vanda macrantha, Chinese renanthera, anoectochilus formosanus or paphiopedilum bulbifer for symbiotic culture, the survival rate of tissue culture seedlings can be remarkably increased under the condition of completely no hormone, and the survival rate of the tissue culture seedlings can be increased. The problem that tissue culture seedlings are prone to death in hormone-free culture is effectively solved, the tissue culture seedlings inoculated with the strain can maintain normal growth, biomass accumulation is stable, and the method has the advantages of being easy and convenient to operate, low in cost and high in seedling survival rate.

The invention belongs to the technical field of plant molecular biology, and particularly relates to a cymbidium sinense CsCCD4 gene and an effect of the cymbidium sinense CsCCD4 gene in aroma regulation. The invention provides a related CsCCD4 gene for synthesizing a substance for enhancing orchid aroma. The nucleotide sequence of the related CsCCD4 gene is shown as SEQ ID NO. 1. According to the invention, the full length of a gene sequence is obtained through cloning, an over-expression vector is constructed on the basis, and instantaneous conversion of butterfly orchid petals and stable conversion of tobacco grandiflora are carried out. The result shows that the substance content of the aroma substance beta-ionone in the instantaneous phalaenopsis petals and the stably converted Bensi tobacco petals is remarkably increased compared with a control group CK, and it is indicated that CsCCD4 plays an important role in enhancing the aroma substance of orchid. As an important CsCCD4 gene in the synthesis process of substances for enhancing orchid aroma, the CsCCD4 gene can be used for improving ornamental characters and creating genetic varieties in orchid genetic engineering.

The application discloses a composition for inhibiting discoloration of ascorbyl glucoside and application of the composition in antioxidant skin care products, and belongs to the field of toiletry preparations. The composition for inhibiting discoloration of ascorbyl glucoside comprises the following components in mass fractions: ascorbyl glucoside 10-15 parts, pine mushroom extract 2-4 parts, Cymbidium goeringii root extract 2-5 parts and astaxanthin 1-3 parts. The composition provided by the application has good stability when ascorbyl glucoside, pine mushroom extract, Cymbidium goeringii root extract and astaxanthin are used in combination, effectively inhibits discoloration of ascorbyl glucoside over time, enhances the antioxidant performance of ascorbyl glucoside, and has important significance for effective application of ascorbyl glucoside in skin care products.

The application discloses a kind of adjustable microclimate film cultivation device for Chinese orchid cultivation, the device is suitable for being arranged in greenhouse environment, including support frame, the support frame is fixedly connected on hardened ground, the support frame top horizontal laying is covered with film plate, the lateral film of support frame side vertical surface can be detachably connected, a plurality of cultivation holes are formed in the film plate, the cultivation hole is inserted with special flowerpot of Chinese orchid, the support frame below is natural soil layer, the support frame around is the hardened ground, the hardened ground has gradient, and the support frame is located at high place.The device has no energy consumption, low cost, solves the multiple needs of Chinese orchid "ventilation-moisture-flood prevention-heat preservation", and is suitable for efficient cultivation of cold-sensitive varieties such as cymbidium goeringii.

This invention provides a tissue culture method for the ornamental aquatic plant *Cephalotaxus fortunei*, including explant preparation: selecting healthy plants free from diseases and pests, cutting stem segments with strong regeneration ability containing 1-2 leaf buds, washing with soapy water, rinsing with tap water for 15 minutes, placing the washed stem segments in a clean bench, soaking in 75% alcohol for 30 seconds, rinsing with sterile water 1-2 times, soaking in 0.1% mercuric chloride solution for 5 minutes, and rinsing with sterile water 3-5 times. Explant inoculation: inoculating the sterilized stem segments into a basic medium formulated with MS + 0.5 mg / L 6-BA + 0.1 mg / L NAA for induction culture. After 30-35 days of culture, differentiated seedlings are obtained. The tissue culture seedlings obtained by the method of this invention have a high proliferation coefficient, are sterile and free from pests and diseases, are uniform and robust, have well-developed root systems, have a long shelf life, and a survival rate of 100%. The overall tissue culture process is simple and easy to operate, shortening the time by more than 20 days compared with traditional methods, which is of great significance to the development of the ornamental aquatic plant industry.