The invention discloses a preparation method of traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica, belonging to the technical field of traditional Chinese medicine preparation methods. At present, the heat-toxin colpitis mycotica is generally treated by antibiotics and sulfonamides, causing great toxic and side effects. The traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica is prepared by the following steps of: soaking fig, Chinese soapberry fruit, soda-apple night-shade herb, Tianqiaomai root, dandelion, dysosma versipellis, root of Indian stringbush, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, longhairy antenoron herb, rohdea japonica root, aster ageratoides, radix sophorae tonkinensis, cremastra appendiculata makino, climbing groundsel herb, huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, herba portulacae, leaf of Chinese coriaria, polygonum hydropiper, sugarcane, hlblsclls llutabilis, radix sophorae flavescentis, bitter gourd, broadleaf holly leaf, elephantopus scaber, rhizoma polygoni cuspidati, saxifraga stolonifera, umbilicaria esculenta, lycoris radiata and liquorice in water; decocting with slow fire; and filtering to remove slag to obtain the liquid medicine. Through the invention, the prepared traditional Chinese medicine has the advantages of little toxic and side effect, short course of treatment and high cure rate.