62 results about "Cremastra appendiculata" patented technology

The invention discloses a method for inducing proliferation of a bud cluster of a protocorm of cremastra appendiculata, wherein the method comprises the following steps: 1) taking the protocorm obtained through germ-free germination of a hand-pollinate seed of the cremastra appendiculata as an explant; 2) putting the protocorm in a minimal medium 1 / 2MS for proliferation culture; 3) putting the proliferated protocorm into a minimal medium 1 / 4MS to induce the bud cluster; 4) cutting an undifferentiated part and a differentiated part of the bud cluster, putting the undifferentiated part and the differentiated part of the bud cluster into different mediums to proliferate the bud cluster and differentiate to emerge a seedling and culture; 5) putting a plant with the emerged seedling in a minimal medium 1 / 4MS for rooting culture. The tissue culture seedling of the cremastra appendiculata is hardened and transplanted to a medium suitable for growth until the seedling grows into a strong cremastra appendiculata plant. The method effectively improves the propagation coefficient of the cremastra appendiculata seedling, thereby laying a foundation for the large-scale production.

The invention belongs to field of crop seed artificial production technology, and provides a key technology for producing rhododendron orchid which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of ghododendron orchid and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

The invention discloses a preparation method of traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica, belonging to the technical field of traditional Chinese medicine preparation methods. At present, the heat-toxin colpitis mycotica is generally treated by antibiotics and sulfonamides, causing great toxic and side effects. The traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica is prepared by the following steps of: soaking fig, Chinese soapberry fruit, soda-apple night-shade herb, Tianqiaomai root, dandelion, dysosma versipellis, root of Indian stringbush, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, longhairy antenoron herb, rohdea japonica root, aster ageratoides, radix sophorae tonkinensis, cremastra appendiculata makino, climbing groundsel herb, huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, herba portulacae, leaf of Chinese coriaria, polygonum hydropiper, sugarcane, hlblsclls llutabilis, radix sophorae flavescentis, bitter gourd, broadleaf holly leaf, elephantopus scaber, rhizoma polygoni cuspidati, saxifraga stolonifera, umbilicaria esculenta, lycoris radiata and liquorice in water; decocting with slow fire; and filtering to remove slag to obtain the liquid medicine. Through the invention, the prepared traditional Chinese medicine has the advantages of little toxic and side effect, short course of treatment and high cure rate.

The invention discloses a cremastra appendiculata seedling method. The method includes seed selection, culture medium preparation, inoculation, culture-room cultivation, seedling hardening, bottle-out cultivation, fertilization and transplantation. Group-culture seedling and seedling hardening method and process are improved, a culture medium formula that group-culture seedlings start to take root in the first stage, the following nearly three-month group-culture time is omitted, the transfer process of twice group-culture seedlings is omitted, a great deal of man power and financial resources are saved, sufficient time is provided for production of the next batch of cremastra appendiculata seedlings, and the cremastra appendiculata seedling method is suitable for promotion and application in the technical biotechnilogies.

A traditional Chinese medicinal composition for treating gout is disclosed in the present invention, the traditional Chinese medicinal composition is composed of radix astragali 10 to 50 weight amount, smilax glabra 20 to 120 weight amount, cyathula officinalis 10 to 50 weight amount, and cremastra appendiculata 5 to 30 weight amount, on the basis, also adding great burdock achene, sparganium stoloniferum, zedoary turmeric clematis root and etc. raw materials. The preparation method of the traditional Chinese medicinal composition raw materials is: weighting raw materials, adding water to decoct 2 to 3 times, each time 5 to 15 times amount, decocting 0.5 to 2 hours, combining medicine liquid, filtering, reduced pressure concentrating the filtering liquid to recovery ethanol, concentrating to relative density is 1.05 to 1.25 detected under 50 to 60 C DEG, drying, obtaining dried cream; grinding dried cream into fine powder, adding proper supplementary to prepare granule, pill, capsule or tablet orally-administrated solid preparations. The traditional Chinese medicinal composition of the present invention can be used for treating gout.

The invention discloses a quick cultivation method for regenerated plantlets of cremastra appendiculata. The quick cultivation method comprises the following steps: (1) culturing cremastra appendiculata corms; (2) selecting explants; (3) inducing adventitious buds; (4) rooting the rootless seedlings; (5) hardening seedlings. According to the invention, the corms on the cremastra appendiculata budbases are used as the explants, the adventitious buds are induced, the rooting culture for the rootless seedlings is performed and the seedlings are hardened, so that the culturing time of the regenerated plantlets of cremastra appendiculata is greatly shortened, a new method is supplied for large-scale reproduction of the regenerated plantlets of the cremastra appendiculata and the problem of shortage of present cremastra appendiculata plant resources and seedling resources can be effectively solved.

The invention belongs to the technical field of medicinal herb propagation and discloses a cremastra appendiculata seed tissue culture and propagation method. The method comprises the following steps:flushing with running water for 10 minutes, and stripping seeds; disinfecting by using 0.1% of mercury bichloride 10min+70% of alcohol 10s; inoculating into MS+2, 4-D1mg / L+BA 1mg / L callus induction;transferring into 1 / 2MS+NAA 1mg / L+BA 1mg / L callus to realize seedling differentiation; culturing under the conditions of 24+ / -1 DEG C and 2000-2100lx, and illuminating 8-10 hours per day; and placingculture bottles of tissue culture seedlings under room-temperature natural lighting conditions for hardening seedlings, cleaning with clean water, planting into a pre-disinfected substrate, and splitcharging by a feeding block. The induction rate can reach 55% within 50 days, the seedlings are grown within 40 days after culture, the survival rate of the seedlings is 83%, the new roots grow earlier, the root system is developed and strong, and plant growth is good.

The invention relates to a medicament for treating epidural post anesthesia lumbago and backache. The medicament comprises the following materials in parts by weight: 10-20 parts of herba dracocephali integrifolii, 10-25 parts of erycibe obtusifolia, 1-15 parts of soldago decurrens lour, 1-10 parts of kamuning, 1-15 parts of saposhnicovia divaricata, 1-10 parts of trichomanes japonicum thunb., 1-10 parts of dried ginger, 1-15 parts of folium isatidis, 1-10 parts of medulla helwingiae, 10-20 parts of cremastra appendiculata, 10-30 parts of rhizoma arisasmatis, 1-20 parts of semen plantaginis, 1-10 parts of kansui root, 10-20 parts of salvia chinensis and 1-10 parts of bighead atractylodes rhizome. The medicament for treating epidural post anesthesia lumbago and backache has the characteristics of being low in cost, easy to prepare, less in toxic and side effects, and obvious in curative effect.

The invention provides a method for accelerating germination of cremastra appendiculata seeds and belongs to the technical field of plant tissue culture. The method comprises the steps of seed selection, seed surface treatment, seed sterilization and seed culture. Plant disinfection solution is mainly adopted for immersing in the step of seed surface treatment and seed sterilization, active ingredients of the plant disinfection solution are glycyrrhizic acid, lemon juice and honeysuckle leaf extract; lycium barbarum leaf juice and moringa oleifera leaf juice are added into a germination culture medium and used as nutrient components, and NAA and GGR6 are used as auxin. According to the method, cremastra appendiculata seeds can be germinated in advance, and the seed germination rate can beimproved.

The invention discloses a method for improving seedling hardening survival rate of tissue culture seedlings of cremastra appendiculata, and particularly relates to the technical field of planting. The method comprises the following steps: selecting mature cremastra appendiculata capsules, picking the cremastra appendiculata capsules, washing the cremastra appendiculatacapsules with running water for surface cleaning, and then placing the cremastra appendiculata capsules in an ultra-clean workbench. Corms at the base parts of buds of the cremastra appendiculata are used as explants, and adventitious buds are induced and rootless seedlings are subjected to rooting culture and seedling hardening, so that the time for culturing regeneration plants of the cremastra appendiculatais greatly shortened, a new way is provided for large-scale propagation of the regeneration plants of the cremastra appendiculata, the cremastra appendiculata cultivated in the mode grows well and is high in survival rate, the technical level in the prior art is remarkably improved, reliable technical guarantee is provided for large-scale and industrialized production of cremastra appendiculataseedlings, a new way is provided for large-scale propagation of the cremastra appendiculata, and the problem of shortage of current cremastra appendiculata plant resources and seedling resources is effectively solved.

The invention discloses a traditional Chinese medicine composition for treating alzheimer disease as well as a preparation method and an application thereof. The traditional Chinese medicine composition is prepared from the following medicinal materials in parts by weight: 2-6 parts of American ginseng, 5-15 parts of angelica sinensis, 7-15 parts of rhizoma corydalis, 4-12 parts of waternut herb, 4-12 parts of cremastra appendiculata, 2-6 parts of schisandra chinensis, 4-12 parts of stephania tetrandra, and 2-10 parts of polygala tenuifolia. The research shows that the traditional Chinese medicine composition can improve the learning and memory abilities of an AD mouse model and the visceral index of the immune organs, improve the permeability of the blood brain barrier of the AD mouse model, reduce the hippocampi APP, Abeta1-42, IL-1beta and IL-6 levels of the AD mouse, enhance the oxidation resistance of the hippocampi of the AD mouse, improve the hippocampi morphological structure of the AD mouse, and reduce the hippocampi RAGE and NF-kappaBp65 expressions of the AD mouse, and has no influences on the body weight of the AD mouse model. The traditional Chinese medicine composition, as well as the preparation method and the application thereof provide a novel technological means for the further research and development on the new traditional Chinese medicine for preventing and treating AD.

The invention provides a method for promoting the germination of Rhododendron orchid seeds, and belongs to the technical field of plant tissue culture. The method includes the steps of seed selection, seed surface treatment, seed disinfection and seed culture. The seed surface treatment and seed disinfection mainly use plant disinfectant for soaking. The active ingredients of the plant disinfectant are acetylshikonin, aloe juice, and oleander leaf extract; okra juice and moringa are added to the germination medium. Leaf juice serves as nutrients and NAA and GGR6 as auxins. The method of the invention can cause the seeds of Rhododendron to germinate early and improve the seed germination rate.

The invention discloses a method for culturing calluses of cremastra appendiculata. The method includes the following steps of explant selection, pre-culture, induction culture of the calluses, and enrichment culture of the calluses. According to the method, a cremastra appendiculata tender scape serves as an explant, a large quantity of good-quality calluses of cremastra appendiculata can be quickly obtained through induction culture and enrichment culture of the calluses, and therefore the problem that the prior art is short of cremastra appendiculata plant resources and medicinal material resources can be effectively solved.

The invention relates to the field of traditional Chinese medicine application and particularly discloses a traditional Chinese medicine for treating the dysfunction of endocrine. The traditional Chinese medicine for treating the dysfunction of the endocrine is prepared by mixing of raw materials of fineleaf schizonepeta herbs, moxa liquoric roots, white muscardine silkworm, notopterygium roots, balloon flowers, Chinese thorowax roots, cremastra appendiculata, piperis longi, lycopus lucidus, flying bandit, oriental wormwood, geranium strictipes, dendrobium, roselle, leonurus macranthus, mioga ginger, crocus sativus, herba hyperici japonici, panax notoginseng, heartleaf houttuynia herbs, coriolus versicolor, Chinese angelica, bighead atractylodes rhizome, white peony roots, cinnamon, morinda officinalis, Chinese magnoliavine fruit, male silkworm moths, sliced aconite, male cloves, fossil fragments, polygonatum, jujubes, alismatis, poria cocos and root bark of the peony tree in certain weight proportions. The traditional Chinese medicine for treating the dysfunction of the endocrine has the advantages of being simple in preparation, good in absorption effect, long in duration of the efficacy remarkable in curative effect, free of side effect, good in safety, low in production cost and beneficial for patients to relieve symptoms in time.

The invention belongs to field of crop seed artificial production technology, and provides a key technology for producing rhododendron orchid which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of ghododendron orchid and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

The invention relates to a high-yield bifoliate cremastra appendiculata seedling cultivation method. The high-yield bifoliate cremastra appendiculata seedling cultivation method comprises the following steps of obtaining pure fruit pods of bifoliate cremastra appendiculata, carrying out softening treatment, disinfection and sterilization on testa of the bifoliate cremastra appendiculata to obtain sterile cremastra appendiculata seeds, sowing the sterile cremastra appendiculata seeds into a specific germination culture medium, carrying out germination culture to obtain light yellow protocorms, transferring the protocorms into a proliferation culture medium for proliferation culture to obtain green proliferation protocorms with white villus appearing on the proliferation surface of the green proliferation protocorms, transferring the green proliferation protocorms into a germination culture medium for differentiation germination culture to obtain rootless seedlings with expanded root pseudobulbs and differentiated clustered buds and a pair of leaves, and transferring the seedlings into a rooting culture medium for rooting culture to obtain tissue culture seedlings with expanded root pseudobulbs and 3-5 root hairs. By means of the cultivation method, a large number of high-yield bifoliate cremastra appendiculata domesticated seedlings can be obtained.

The invention relates to traditional Chinese medicine for treating tumors and a preparation method of traditional Chinese medicine. The traditional Chinese medicine comprises plant raw materials and animal raw materials by weight, wherein the plant raw materials are 22 to 35 parts of peach kernel, 12 to 20 parts of acanthopanax senticosus, 15 to 19 parts of cremastra appendiculata, 10 to 15 parts of herba gynostemmatis pentaphylli, 20 to 30 parts of astragalus membranaceus, 32 to 36 parts of semen cuscutae, 12 to 18 parts of radix sophorae flavescentis, 5 to 15 parts of giant knotweed, 8 to 15 parts of juncus effusus, 20 to 30 parts of arenaria brevipetala, 23 to 28 parts of discolorous clinquefoil root, 30 to 34 parts of ginkgo leaves, 20 to 25 parts of aconitum szechnyianum gay, 30 to 36 parts of asparagus cochinchinensis, 28 to 35 parts of starjasmine stem, 8 to 15 parts of fructus polygoni orientalis, 3 to 13 parts of selfheal, 30 to 50 parts of honeysuckle stem, 10 to 18 parts of rhizoma dioscoreae nipponicae, and 8 to 10 parts of uncaria rhynchophylla; theanimal raw materials are 3 to 5 parts of lumbricus, 4 to 8 parts of sea crab shell, and 2 to 5 parts of centipede; the weight ratio of the plant raw materials to the animal raw materials is 1:(0.01 to 0.05). According to the characteristics of drug property compatibility, the traditional Chinese medicine has the advantages that under the condition of inhibiting growth of tumor cells, the organic immunity and resistibility can be improved; the phagocytic function of macrophage can be promoted; the content of white blood cells can be increased; the haematopoietic function of bone marrow is protected; the ill symptoms and signs of patients can be improved.

The invention discloses an ozostomia-treating pulvis which is characterized by comprising the following ingredients, by weight, 25-35 parts of Radix Codonopsis, 25-35 parts of bighead atractylodes rhizome, 20-30 parts of Poria cocos, 20-30 parts of licorice, 20-30 parts of Cremastra appendiculata Makino, 25-35 parts of dried orange peel and 25-35 parts of barbat skullcap. The medicine provided by the invention contains pure Chinese herbal components, is mainly used for treating ozostomia, especially ozostomia caused by stomach Qi, has a remarkable curative effect on long-term ozostomia, and has efficacy of benefiting the stomach, tonifying spleen, nourishing the stomach and the like.

The invention provides a cancerous pain treating traditional Chinese medicine agent, which comprises radix aconiti, radix aconiti kusnezoffii, arisaema heterophyllum blume, pinellia ternata, ricinus communis, semen sinapis, semen strychni, flos rhododendri mollis, garden balsam seed, hyoscyamus niger l, gamboge, fructus polygoni orientalis, curcuma longa l, halitum purpureum, piper nigrum l, cremastra appendiculata pseudobulb, stellera chamaejasme, borneol, calomel, radix paeoniae alba and licorice root. According to the present invention, the cancerous pain treating traditional Chinese medicine agent has characteristics of reasonable compatibility, simple and convenient administration, external treatment of internal illness, pain removing and life prolonging, has effects of blood circulation activation, blood stasis removing, body fluid regeneration, toxic substance removing, swelling subsiding, pain stopping, heat clearing, mass softening, anticancer and antibacterial effect, and canprovide good treatment effects on various cancerous pains.

The invention discloses a medicine for treating gastric cancer and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. The medicine disclosed by the invention is prepared from the following raw materials in parts by weight: 30 parts to 60 parts of radix pseudostellariae, 30 parts to 60 parts of radix astragali, 10 parts to 20 parts of radix glycyrrhizae, 20 parts to 30 parts of herba dendrobii, 10 parts to 20 parts of fructus mume, 15 parts to 30 parts of parched radix paeoniae alba, 20 parts to 60 parts of herba scutellariae barbatae, 30 parts to60 parts of herba duchesneae indicae, 10 parts to 30 parts of lizard, 10 parts to 30 parts of fructus aurantii, 3 parts to 6 parts of radix notoginseng powder, 15 parts to 30 parts of cremastra appendiculata, 30 parts to 90 parts of coix seeds, and 10 parts to 30 parts of radix scutellariae. For 50 cases adopting the medicine for the treatment of gastric cancers, 31 cases take effects after one treatment course, 12 cases are effective, 7 cases are ineffective, and the overall response ratio is up to 86 percent. The medicine for treating the gastric cancer has the advantages that clinical symptoms of the gastric cancer can be improved, the curative effect is definite, and the side effects are small.

The invention relates to a traditional Chinese medicine for treating cancer and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine provided by the invention comprises the following raw materials in parts by weight: hedyotis diffusa willd, sculellaria barbata, caulis spatholobi, venenum bufonis, berchemia lineate, young-geun kim, fructus xanthii, phymatopsis hastate, eucommia ulmoides, radix fici simplicissimae, keel, kadsura pepper stem, cedrela sinensis root, semen oroxyli, cremastra appendiculata makino, fructus ligustri lucidi and ginseng. The traditional Chinese medicine has the effects of removing blood stasis, detoxifying, eliminating evil, diminishing inflammation, tonifying spleen, harmonizing stomach, tonifying Qi, tonifying spleen, invigorating kidney, balancing Yin and Yang, enhancing immunity and the like and has a better curative effect on various cancers.

The invention discloses a method for increasing proliferation coefficient by increasing and jointing pseudobulb of cremastra appendiculata. The method comprises the following steps: firstly, inoculating protocorm of the cremastra appendiculata into a cluster bud induction culture medium for culturing, obtaining cluster buds after 30 days, adding a sterilized pseudobulb inducer and joint increasing and jointing inducer, and forming clustered pseudobulb from the cluster buds after 60 days, wherein the number of the pseudobulb sections is 2-6, and the distance between the joints is long; and cutting the clustered pseudobulb of the cremastra appendiculata into single pseudobulb, transversely cutting each pseudobulb into a stem section with one stem node, and respectively inoculating the stem sections into a proliferation culture medium to achieve the purpose of improving the proliferation coefficient of cremastra appendiculata. According to the method, the pseudobulb and the joint increasing and jointing inducer thereof are added to the culture medium of the cremastra appendiculata cluster buds, so that the number of joints on the pseudobulb is increased, the distance between the joints is increased, and the multiplication coefficient can be increased by 2.7-4.1 times on the original basis and can reach 21.32 to the maximum. The method is good in repeatability, safe, efficient, energy-saving and consumption-reducing, and provides technical support for applying the tissue culture and rapid propagation technology of cremastra appendiculata to industrialized production.

The invention discloses a traditional Chinese medicine composition for treating chronic nephritis. The traditional Chinese medicine composition comprises the following Chinese herbal raw materials in parts by weight: 10-30 parts of eleutherococcus senticosus, 10-20 parts of eucommia ulmoides, 5-15 parts of rhizoma zingiberis, 10-20 parts of semen plantaginis, 10-20 parts of astragalus membranaceus, 5-15 parts of rhizoma atractylodis, 10-20 parts of bluish dogbane, 10-20 parts of cremastra appendiculata, 10-20 parts of radix morindae officinalis, 5-15 parts of serissa japonica, 10-20 parts of lalang grass rhizome, 10-20 parts of rhizoma polygonati, 5-15 parts of cortex mori radicis, 10-20 parts of herba houttuyniae, 10-20 parts of common achyranthes herb, 10-20 parts of radix ophiopogonis, 5-15 parts of rhubarb, 10-20 parts of crotalaria ferruginea Grah, 10-20 parts of corn silk, 10-20 parts of pericarpium arecae, 5-15 parts of soapstone, 10-20 parts of madder, 10-20 parts of sculellaria barbata and 5-15 parts of radix glycyrrhizae. The traditional Chinese medicine composition has the efficacies of invigorating spleen and reinforcing kidney, clearing heat and drying dampness, relieving internal heat and dispelling wind, and inducing diuresis and alleviating edema, and is obvious in the curative effect of treating chronic nephritis.

The invention discloses a method for inducing proliferation of a bud cluster of a protocorm of cremastra appendiculata, wherein the method comprises the following steps: 1) taking the protocorm obtained through germ-free germination of a hand-pollinate seed of the cremastra appendiculata as an explant; 2) putting the protocorm in a minimal medium 1 / 2MS for proliferation culture; 3) putting the proliferated protocorm into a minimal medium 1 / 4MS to induce the bud cluster; 4) cutting an undifferentiated part and a differentiated part of the bud cluster, putting the undifferentiated part and the differentiated part of the bud cluster into different mediums to proliferate the bud cluster and differentiate to emerge a seedling and culture; 5) putting a plant with the emerged seedling in a minimal medium 1 / 4MS for rooting culture. The tissue culture seedling of the cremastra appendiculata is hardened and transplanted to a medium suitable for growth until the seedling grows into a strong cremastra appendiculata plant. The method effectively improves the propagation coefficient of the cremastra appendiculata seedling, thereby laying a foundation for the large-scale production.

The invention provides a method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS. The method comprises the following steps: artificial cultivation conditions of the rhododendron, extraction of the chemical components of the pseudobulbs, preparation of a standard sample, preparation of a sample to be detected, liquid phase detection conditions, mass spectrum detection conditions, ion source setting and qualitative analysis of the chemical components. According to the method for detecting the chemical components of pseudobulbs of Cremastra appendiculata, 9 chemical components of colchicine, quercetin, adenosine, rutin, emodin, rheinic acid, physcion, myristic acid and oleanolic acid of the Cremastra appendiculata pseudobulb extract can be rapidly, accurately and sensitively detected; the method overcomes the defects of few chemical component detection methods, undefined effective components, difficulty in evaluating the internal quality of the medicinal material and the like, and provides guidance for evaluating the internal quality of the Cremastra appendiculata medicinal material.

The present invention discloses a preparation for gout, soft tissue injury and rheumatalgia, and a preparation method thereof, wherein the preparation comprises the following raw materials by weight: 20-40 parts of clematis chinensis osbeck, 20-60 parts of zanthoxylum nitidum, 10-30 parts of cremastra appendiculata, 20-40 parts of panax notoginseng, 20-60 parts of angelica sinensis, 60-100 parts of curcuma longa l, 20-40 parts of cape jasmine, 10-30 parts of safflower, and 10-30 parts of rhizoma paridis. According to the present invention, the preparation has effects of heat clearing, blood cooling, wind evil expelling, dampness removing, blood circulation activation, blood stasis removing, tendon relaxing and activating, swelling subsiding and pain stopping, can provide good treatment effects for gout, soft tissue injury and rheumatalgia, and further has characteristics of convenient use, safety, no toxicity, and no side effect; and the preparation method has characteristics of simple process, low production cost and low energy consumption, and is suitable for industrial production.

The invention discloses a new method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino.The method comprises the following steps: (1) obtaining an explant; (2) firstly washingsoil on the surface of pseudobulb in step (1) with tap water, then soaking with a washing powder solution, then rinsing with running water, then treating with an ethanol solution, and rinsing after disinfection; (3) obtaining sterile seedlings; (4) transferring young shoots grown from buds cultured in step (3) to a medium A in step (3) for enrichment culture, cutting off seedlings with callus, andcontinuing to transfer to a medium B for culture; (5) inoculating a medium C with single seedlings with rice grain-sized pseudobulb after subculture in step (4) for culture; (6) exercising the seedlings and transplanting. The method disclosed by the invention has the advantages that the in-vitro rapid propagation of the cremastra appendiculata (D.Don) Makino is optimized and adjusted; the processof artificial rapid propagation is simplified; the propagation efficiency is greatly improved; the cost is low and the time is short; not only is the survival rate of tube seedlings through seedlingexercising improved, but the nursery cycle is also greatly shortened.

The invention discloses passiflora fruit nutritive soybean milk, which is prepared from the following raw materials of passiflora fruit, soybean, Chinese ixeris herb, mung bean, water, rape oil, cremastra appendiculata pseudobulb, honeysuckle flower, oenothera root, water chestnut leaf, mentha spicata herb, fructose and spiral seaweed powder. The preparation method comprises the following steps: (1) preparing passiflora fruit juice, (2) preparing mixed juice, (3) preparing mung bean flour, (4) preparing nutrient solution, (5) preparing soybean milk, (6) mixing the mixed juice, the mung bean flour, the nutrient solution, the soybean milk and the spiral seaweed powder, adding the fructose and sterilizing after homogeneity to obtain the passiflora fruit nutrition soybean milk. By introducing the nutrient ingredients of the passiflora fruit and the hinese ixeris herb into the soybean milk and adding the health-care beneficial components of the cremastra appendiculata pseudobulb and the spiral seaweed powder, the passiflora fruit nutrition soybean milk is soft and smooth in taste and unique in flavor, contains various kinds of nutrient ingredients of protein, amino acid and microelements, is easy to digest and absorb and has better-dietary therapy and health-care effects.

The invention relates to a formula and a preparation method of Hui traditional Chinese medicine cremastra appendiculata liver cancer treating particles. According to the present invention, the Hui traditional Chinese medicine cremastra appendiculata liver cancer treating particles are the green high-tech Chinese herb product for liver cancer treatment; cremastra appendiculata, camptotheca acuminata, American ginseng, oyster, cirsium japonicum, radix paeoniae rubra and other Chinese herbs are mixed according to a certain ratio, water is added, and decoction, extraction, concentration and granulation are performed to obtain the traditional Chinese medicine particles; and the Hui traditional Chinese medicine cremastra appendiculata liver cancer treating particles mainly treat liver cancer, can further treat throat cancer, stomach cancer, large intestine cancer and other digestive system cancers, and have characteristics of moderate components and moderate doses, wherein the modern medical pharmacology experiment results prove that the selected medicines can treat cancers, and the body functions are fundamentally improved, the condition deteriorating is prevented, and the health body is recovered in the low cost manner.