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59 results about "Ostrea gigas" patented technology

Method for quickly detecting content of glycogen and protein of crassostrea gigas

The invention discloses a method for quickly detecting the content of glycogen and protein of crassostrea gigas. The method comprises the following steps: firstly, homogenizing a to-be-detected sample; then, collecting near infrared spectrum data; and finally comparing with a pre-established crassostrea gigas glycogen and protein content near infrared model to acquire a measurement value of the to-be-detected sample. The method has the characteristics of high detection speed, no chemical reagent, low experiment cost and no environmental pollution in the process of analyzing the content of glycogen and protein of crassostrea gigas. Establishment of the method for quickly detecting content of glycogen and protein of crassostrea gigas has important meaning to crassostrea gigas meat quality analysis, meat property breeding, breeding generation identification and genetic resource evaluation.
Owner:山东省海洋资源与环境研究院

Method for screening pacific oysters capable of increasing content of unsaturated fatty acid C20:3omega6 and related SNP (Single Nucleotide Polymorphism) primer pair

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for identifying pacific oyster individuals capable of increasing content of an unsaturated fatty acid epoxyeicosatrienoic acid (C20:3omega6) and development and application methods thereof. By virtue of early genome-wide association study, 38 clustered SNP signals in significant correlation with the content of C20:3omega6 located on chromosome 1 of the pacific oysters are identified. A corresponding detection method is developed aiming at one SNP site at the base scaffold 460_104,049 of the area, two base forms A / T exist at the site, and a nucleotide sequence of the upstream / downstream 500bp is shown as SEQ ID No.1. According to the detection method, AT genotype maternal oysters are screened to be used for guiding oyster breeding. The invention provides a method for performing genotype identification on the maternal oysters before fry breeding and increasing the content of the unsaturated fatty acid in the offspring. The result is an SNP marker obtained based on the genome-wide association study, and the effect is stable.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Multifunctional food composition capable of increasing bone density and preparation method of multifunctional food composition

The invention belongs to the technical field of health-care foods, and particularly relates to a multifunctional food composition capable of increasing bone density and a preparation method of the multifunctional food composition. The multifunctional food composition consists of the following components in parts by weight of 20-25 parts of dried roots of pueraria lobate, 12-20 parts of Chinese wolfberry fruits, 10-15 parts of dried roots and stems of polygona tum kingianum, polygonatum sibiricum or polygonatum cyrtonema, 4-6 parts of dried roots and stems of dioscorea opposite, 4-6 parts of dried ziziphus jujuba, 2-3 parts of shell of ostrea gigas or ostrea talienwhanensis crosse or ostrearivularis gould, 2-4 parts of colla corii asini, 2-4 parts of rhizoma polygonati odorati, 1-3 parts ofporia cocos, 1-3 parts of dried roots and stems of glycyrrhiza uralensis, or glycyrrhiza inflata or glycyrrhiza glabra, 1-3 parts of dried mature Chaenomeles speciosa and 1-2 parts of coix lacryma-jobi. The raw drug materials have medicinal and edible functions, and have notable curative effects through osteoporosis-resistant experiment on animals. Compared with an estrogens medicine through clinical application, the multifunctional food composition has similar drug effects, and adverse reactions on the liver, the kidney and the like can be notably reduced. Besides, the multifunctional food composition can assist in reducing blood lipid, and improve memory.
Owner:沈阳百岁生物科技发展有限公司

Crassostrea gigas elongation factor-1-alpha (EF-1-alpha) promoter, and recombinant vector and application thereof

ActiveCN110684769APerfecting gene editing technologyPeptidesNucleic acid vectorBiotechnologyEmbryo
The invention discloses a crassostrea gigas elongation factor-1-alpha (EF-1-alpha) promoter as well as a recombinant vector and application thereof. The invention provides an EF-1-alpha promoter. TheEF-1-alpha promoter is a high-expression housekeeping gene promoter which can be expressed in early stage of embryo development of crassostrea gigas. The EF-1-alpha promoter is specifically expressedas follows: designing a specific primer; amplifying a fragment of the crassostrea gigas EF-1-alpha promoter; inserting the crassostrea gigas EF-1-alpha promoter into a vector containing a GFP reportergene by utilizing In-fusion connection so as to obtain a recombinant vector; and then, introducing the recombinant vector into fertilized eggs of crassostrea gigas, thereby efficiently driving expression of the GFP reporter gene in embryos and larvae of crassostrea gigas. A universal-type efficient promoter sequence for crassostrea gigas is prepared according to the invention; and thus, importanttechnical basis is provided for development of CRISPR / cas9 targeted gene editing technology.
Owner:OCEAN UNIV OF CHINA

Method for cultivating new, convex-shell and thin-shell strain of Ostrea rivularis Gould in north

InactiveCN109122439AOvercoming small shell convexityShell convexMicrobiological testing/measurementClimate change adaptationThin shellsGonad
The invention provides a method for cultivating a new, convex-shell and thin-shell strain of Ostrea rivularis Gould in the north. The method comprises the steps: collecting parent oysters of Crassostrea gigas and northern Ostrea rivularis Gould firstly, performing synchronous maturity promoting cultivation, when gonads of the parent oysters of Crassostrea gigas and northern Ostrea rivularis Gouldare mature synchronously, performing acquisition of mature eggs of Ostrea rivularis Gould and sperms of Crassostrea gigas, performing hybridization and fertilization so as to obtain a hybrid filial generation of the parent oysters, cultivating the filial generation after the hybrid group is obtained, adopting a microsatellite molecular marker technique to confirm that the first hybrid filial generation is the first filial generation of hybridization of Crassostrea gigas and northern Ostrea rivularis Gould, then adopting a truncated selection method to cultivate the first filial generation for1-2 years in a northern breeding sea area, and performing continuous one-to-one selfing on the hybrid strain so as to obtain the new, convex-shell and thin-shell strain of Ostrea rivularis Gould. Theobtained new strain of Ostrea rivularis Gould has the advantages of large shell convexity, thin shells, fast growth, high meat yield and the like.
Owner:OCEAN UNIV OF CHINA

Cup-shaped long oyster new strain cultivation method

ActiveCN108012964AAchieving Collaborative ChoiceHigh yieldClimate change adaptationPisciculture and aquariaOysterBiology
The invention provides a cup-shaped long oyster new strain cultivation method. The method comprises the steps that a group of which the shell shape is cup-shaped, the oyster cup depth is more than 3 cm, and the meat yield rate is high is selected as a core group; 10%of long oyster groups of the core group of which the shell shape is cup-shaped, the oyster cup depth is more than 3 cm, and the meatyield rate is high are selected through a truncating selection method as a preferred group and a breeding group to obtain a cup-shaped long oyster new strain F1; a character that the shell shape is cup-shaped is further purified to form a second-generation breeding family strain F2 through a family strain breeding method; finally, a new strain with stable characters is obtained through successivepurification of a number of generations. The cup-shaped long oyster strain cultivated by the method has the advantages of stable inheritance, high meat yield, extremely high commercial value and the like, and a huge prospect of popularization and breeding.
Owner:OCEAN UNIV OF CHINA

Recombinant serum amyloid A capable of enhancing immune response of crassostrea gigas, and preparation method thereof

The invention discloses recombinant serum amyloid A capable of enhancing immune response of crassostrea gigas. The recombinant serum amyloid A is characterized that the amino acid sequence is represented by SEQ ID NO. 1. Gene expression of cytokines CgIL17-1, CgIL17-5, and CgTNF in blood lymphocytes can be remarkably promoted inside and outside bodies of the crassostrea gigas, the immune responselevel of the crassostrea gigas can be enhanced, and the recombinant serum amyloid A has application value in preparation of novel immune preparations and other drugs for shellfishes, especially breeding shellfishes.
Owner:DALIAN OCEAN UNIV

Cultivation method for rapidly growing triploid crassostrea gigas Haida No.1

The invention provides a cultivation method for rapidly growing triploid crassostrea gigas Haida No.1. The method comprises the following steps: obtaining high-heterozygosity triploid crassostrea gigas Haida No.1 by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas Haida No.1, taking females of the triploid crassostrea gigas Haida No.1 as female parents, taking males of the diploid Haida No.1 as male parents, and obtaining tetraploid crassostrea gigas Haida No.1 with improved growth characters by inhibiting discharge of the first polar bodies of the fertilized eggs; and hybridizing males of the tetraploid crassostrea gigas Haida No.1 and the diploid crassostrea gigas Haida No.1 to obtain the triploid crassostrea gigas Haida No.1 which grows quickly. According to the method, the growth performance of the crassostrea gigas is improved by adopting a method for improving the heterozygosity of parent genes, and the tetraploid crassostrea gigas Haida No.1 obtained by adopting the method is hybridized with the diploid crassostrea gigas Haida No.1 which originally has the characteristic of rapid growth to obtain the triploid crassostrea gigas Haida No.1 with rapid growth performance.
Owner:OCEAN UNIV OF CHINA

Induction method of high-heterozygosity tetraploid of new variety of crassostrea gigas 'Haida No.3'

PendingCN112931323AIncrease gene heterozygosityImprove slow growthClimate change adaptationPisciculture and aquariaPolar bodyOstrea gigas
The invention provides an induction method of a high-heterozygosity tetraploid of a new variety of crassostrea gigas 'Haida No.3'. The method comprises the following steps: taking female triploid crassostrea gigas 'Haida No.3' obtained by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas 'Haida No.3' as a female parent; taking a male diploid 'Haida No.3' as a male parent; after the female parent and the male parent are artificially matured, putting ova into seawater to be matured, and when the ova are round through microscopic examination, the ova are matured; conducting insemination on the cured ova and sperms, starting timing after the sperms and the ova are mixed, treating fertilized ova with cytochalasin B, inhibiting discharging of the first polar bodies of the fertilized ova, and collecting and soaking the fertilized ova; and transferring the fertilized ova into a cultivation container for incubation and larva cultivation. The triploid female parent shellfish is obtained by inhibiting the discharge of the first polar bodies of the fertilized ova of the diploid crassostrea gigas 'Haida No.3', and the tetraploid obtained by the triploid female parent shellfish has higher growth advantage due to higher gene heterozygosity.
Owner:OCEAN UNIV OF CHINA

A Breeding Method of Long Oyster with High Melanin Content in Soft Body

The invention discloses a breeding method of crassostrea gigas (black oysters) with a high melanin content in the soft parts and a proof scheme for the effectiveness of the method. The breeding method includes the following steps that by observing the color of common oysters, the oysters of which the ratio of the area of black shells to the total shell area is more than 90 percent and the lightness value V< 0.20 are selected, melanin extraction of oyster shells, melanin extraction of soft parts of the oyster shells and relation analysis of the melanin content and the melanin content of the soft parts are carried out, the applicant finds a significant positive correlation phenomenon of the color of the shells of the crassostrea gigas and the deposit rate of melanin in the pallium in the experiment process, and the blacker the shells are, the higher the melanin content of the soft parts is. By means of the breeding method of the crassostrea gigas (black oysters) with the high melanin content in the soft parts, the oysters with the high melanin content in the soft parts can be selected and bred rapidly, the breeding efficiency of the oysters is improved, and as the health-care value of melanin is accepted by the market, the new oyster species obtained through the method can increase the additional value of breeding.
Owner:LUDONG UNIVERSITY

Method for evaluating green weight of soft part of ostrea gigas thunb

The invention discloses a method for evaluating the green weight of the soft part of ostrea gigas thunb. The method comprises the steps that the shell height H, shell length L and shell width W of the ostrea gigas thunb are measured; the product of HLW of the shell height H, shell length L and shell width W of the ostrea gigas thunb is calculated; the bigger the HLW value is, the bigger the green weight of the soft part of the ostrea gigas thunb is. According to the method for evaluating the green weight of the soft part of the ostrea gigas thunb, the green weight of the soft part can be reflected more accurately, the ostrea gigas thunb selection and breeding efficiency is improved, the breeding process of the ostrea gigas thunb is accelerated, the breeding precision of the ostrea gigas thunb is improved, the breeding target is strengthened, so that the genetic traits of monomer are more stable and the economic benefits are maximized.
Owner:LUDONG UNIVERSITY

Environment-friendly green and efficient insecticide

The invention discloses an environment-friendly green and efficient insecticide. The environment-friendly green and efficient insecticide is composed of the following components in parts by weight: 0.1-0.2 part of aschersonia powder, 0.5-1.0 part of bacillus thuringiensis var powder, 0.1-0.2 part of bacillus thuringiensis powder, 0.5-1.5 parts of candida powder, 1-3 parts of perilla, 2-4 parts of caesalpinia sappan linn, 2-4 parts of perilla leaves, 1-2 parts of radix paeoniae rubra, 3-4 parts of daphne genkwa, 4-6 parts of fructus amomi, 2-4 parts of almonds, 2-4 parts of eucommia ulmoides, 2-4 parts of holly root, 2-4 parts of ostrea gigas thunberg, 1-2 parts of citrus medica sarcodactylis, 2-4 parts of tortoiseshells, 5-8 parts of aconitum carmichaeli debx, 3-8 parts of coptis chinensis, 1-3 parts of peppers, 5-6 parts of L-nicotine, 2-4 parts of peppers, 5-9 parts of poncirus trifoliata, 9-13 parts of stemona sessilifolia, 15-20 parts of szechwan chinaberry fruit, 4-10 parts of camphor, 1-5 parts of an emulsifying agent and 2-3 parts of turpentine. The green pesticide is a pollution-free pesticide and is friendly to the environment.
Owner:宁贻伟

Health-maintenance health-care coffee and preparation method thereof

InactiveCN112401038AImprove Qi and blood deficiencyImprove fatigue and sleepinessAntinoxious agentsRoasted coffee treatmentHouttuyniaLepidium meyenii
The invention discloses health-maintenance health-care coffee and a preparation method thereof. The health-maintenance health-care coffee is prepared from the following raw material compositions: coffee powder, Lepidium meyenii Walp., ostrea gigas tnunb, ginseng radix, fructus lycii, rhizoma polygonati, euryales semen, radix puerariae lobatae, semen cuscutae, herba taxilli, radix achyranthis bidentatae, Ziziphus jujuba Mill., herba cistanches, radix isatidis, herba houttuyniae, herba portulacae and cortex eucommiae. The health-maintenance health-care coffee disclosed by the invention is actually a coffee beverage capable of tonifying kidney and nourishing, can be drunk for a long time as the beverage, is conveniently taken, is capable of warming and tonifying kidney and eliminating deficiency of qi and blood of the body, fatigue and drowsiness, has an obvious function of nourishing and soothing the nerves, can enhance physical fitness and improves immunity and working efficiency.
Owner:林幼芬

Breeding method of stress-resistant crassostrea gigas new variety

The invention provides a breeding method of a stress-resistant crassostrea gigas new variety. The method comprises the steps that 1, crassostrea gigas with regular shell shapes is selected, and elimination selection is carried out; 2, parent shellfishes with the strongest stress resistance are selected out, and dissecting is carried out after artificial maturation promotion to obtain sperms and ova; 3, after being cleaned, the ova are put into seawater filtered by sand to be cured; 4, one-to-one fertilization is carried out on the cured ova and sperms in sand-filtered seawater to establish families; and 5, the fertilized ova of the different families after fertilization are put into different plastic barrels, the fertilized ova are incubated in sand-filtered seawater, and larva cultivationis carried out. According to the method, along with prolonging of oyster culture time and year-by-year increasing of the oyster death rate, the advantages of the new variety are more remarkable, thelower death rate enables farmers to try to culture oysters of two years old or above, large-size and high-quality oysters more popular with consumers, especially e-commerce, can be produced, and the method has great popularization value.
Owner:烟台和丰水产科技有限公司

Novel technique for preparing oyster shell active calcium capsules

The invention relates to a new production process and a new preparation of an edible acidic active calcium capsule, belonging to the technology field of Chinese medicinal preparations. The edible acidic active calcium capsule is prepared from the shells of Ostrea gigas Thunberg, Ostrea talienwhanensis Crosse or Ostrea rivularis Gould belonging to the family Ostreidae, and thallus of Laminaria japonica Aresch belonging to the family Laminariaceae. Since Laminaria japonica Aresch powder is added into the capsule, and the calcium content in Laminaria japonica Aresch is high, the edible acidic active calcium capsule has good calcium supplementing effect; and because of dietary fiber in the Laminaria japonica Aresch, the capsule can prevent adverse reactions of conventional oyster calcium carbonate preparation, such as constipation, belching and abdominal discomfort. The capsule has the advantages of rich nutrition, easy absorption, scientific and reasonable formula, and convenience for production, transportation, storage, carrying and consumption.
Owner:SHANDONG UNIV AT WEIHAI

Fattening method and device for crassostrea gigas cultured in north

The invention provides a fattening method and device for crassostrea gigas cultured in the north. The fattening method comprises the following steps: selecting a fattening sea area; selecting to-be-fattened crassostrea gigas: selecting crassostrea gigas which is bred in a raft hanging mode and has the fullness degree smaller than 10%; uniformly loading the to-be-fattened crassostrea gigas into the fattening device; putting the fattening device into the fattening sea area: transporting the fattening device loaded with the to-be-fattened crassostrea gigas to the selected fattening sea area for fattening, wherein the fattening device is hung on a culture raft frame; and conducting management in the fattening period. The fattening method and device for the crassostrea gigas cultured in north solve the problems of crassostrea gigas accumulation and poor water flow in the traditional crassostrea gigas fattening process; and the ingestion and growth of the fattening crassostrea gigas are facilitated, the fattening effect of the cultured crassostrea gigas is improved, compared with a traditional breeding fattening technology, the fattening survival rate can be increased by 20-30%, the fattening time is shortened by 15-20 days, and the quality and yield of the cultured crassostrea gigas are remarkably improved.
Owner:OCEAN UNIV OF CHINA

Crassostrea gigas breeding method capable of increasing meat percentage

The invention provides a crassostrea gigas breeding method capable of increasing meat percentage, which comprises the following steps: S1, selection of parent crassostrea gigas: screening crassostrea gigas with golden yellow left shell color from a crassostrea gigas wild population as a basic population, and taking the golden yellow shell color and growth speed as breeding targets; S2, secondary optimization: reselecting 30-35 male and female crassostrea gigas with golden left shell and high meat percentage from offspring propagated by the parentcrassostrea gigas population selected in the S2, and selecting 30-35 male and female crassostrea gigas with golden left shell and high meat percentage from the offspring propagated by the parent crassostrea gigas population selected in the S2; S3, porpulation mating: performing mutual mating on the male and female crassostrea gigas selected in the S1; and S4, second breeding: performing male and female single-pair single mating propagation on the crassostrea gigas with offspring whaving golden yellow shell color and high growth speedin the S2. According to the crassostrea gigas breeding method capable of increasing meat percentage, the crassostrea gigas is formed through continuous breeding of at least four generations by adopting a mixed breeding technology combining family breeding and group breeding, and in the same breeding environment, compared with unbred crassostrea gigas, the average shell height, the average weight and the meat percentage of 15-month-old crassostrea gigas are improved respectively.
Owner:湛江华南贝类研究院

Artificial seedling breeding technology for Portuguese oyster triploid

The invention relates to the technical field of seedling breeding, in particular to a Portuguese oyster triploid artificial seedling breeding technology, which comprises the following steps: hybridizing cultivated crassostrea gigas tetraploid and cultivated Portuguese oyster diploid to obtain Portuguese oyster triploid fertilized eggs, cultivating the Portuguese oyster triploid fertilized eggs to juvenile mollusks, and culturing the juvenile mollusks to obtain the Portuguese oyster triploid artificial seedling. The juvenile mollusks are cultivated into Portuguese oyster triploid adults; proper temperature and salinity are provided for culture of the Portuguese oyster triploid, and the cultured Portuguese oyster triploid has a good survival rate.
Owner:广鸿源(广东)水产有限公司

Fertility recovery method for oyster distant hybrid sterility

ActiveCN103719000ASolve the problem that the follow-up breeding work cannot be carried outClimate change adaptationPisciculture and aquariaOstrea gigasGenotype
The invention discloses a fertility recovery method for oyster distant hybrid sterility. The fertility recovery method includes by a distant hybridization mode, performing synchronous gonad treatment on Hongkong oysters and pacific oysters, and performing interspecific oyster hybridization to acquire a highly-sterile filial generation F1; during maturity seasons of filial generation F1 hybrids, analyzing fertility of the hybrids and screening out fertile individuals; by a family or group reproduction mode, performing self-reproduction of the fertile individuals of the hybrids F1 to acquire a secondary filial generation F2; through molecular markers, screening out individuals relatively similar with the hybrids F1 in terms of genotype from completely-fertile hybrids F2 to acquire completely-fertile groups with characteristics of the hybrids F1 so as to realize complete hybrid fertility recovery. By the fertility recovery method, the problem of failure to continue follow-up breeding work due to oyster intersterility frequently caused during distant hybridization is solved, data and references are provided for distant backcross, multiple cross, potential estimation on hybrid offspring breeding and the like of oysters, and possibility is also provided for research and development of new species of the oysters.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Earthworm polypeptide health-care wine and preparation technology

The invention discloses earthworm polypeptide health-care wine and a preparation technology, and relates to the technical field of health-care wine. The health-care wine comprises the following components in weight ratio: 85 to 90 percent of Baijiu, 1.5 to 2.5 percent of earthworm polypeptide, 2 to 3 percent of rehmannia glutinosa, 2 to 3 percent of ostrea gigas thunberg, 1 to 1.5 percent of platycladus orientalis, 1 to 1.5 percent of ziziphus jujube, and 1 to 1.5 percent of asini corii colla, wherein the alcohol content of the Baijiu is greater than 55 percent; the earthworm polypeptide is an earthworm protein extract. An earthworm polypeptide component with the effect of sleep improvement is extracted from an earthworm material through the technologies of flash extraction, bienzyme hydrolysis, ozone deodorization and ultrafiltration, and is prepared into the health-care wine with obvious efficacy and small side effects through the combination with the traditional Chinese medicine components having the effect of sleep improvement, such as the rehmannia glutinosa, the ostrea gigas thunberg, the platycladus orientalis, the ziziphus jujube and the asini corii colla; and the health-care wine can effectively improve dyssomnia, and is suitable for most of crowds, high in practicability, practical, safe and reliable.
Owner:JIANGSU BULONG BIOTECH

Breeding method of a new strain of Kumamoto oyster resistant to low temperature in the north

The present invention provides a method for cultivating a new strain of low temperature resistant Crassostrea sikamea in the North, wherein Crassostrea sikamea and Crassostrea gigas that belong to Crassostrea are selected; by use of Crassostrea sikamea and Crassostrea gigas with different temperature tolerance and salt tolerance, through hybridization and then selfing, purification, hybridization,and selfing for multiple generations of breeding, finally the new strain of low temperature resistant Crassostrea sikamea is obtained. Practice has proved that the outstanding advantages of the low temperature resistant Crassostrea sikamea obtained by the use of the method in farming are improved low temperature resistance, high survival rate, and greatly increased growth rate, and compared withordinary varieties of Crassostrea sikamea, the growth rate can be increased by 30%, the survival rate is increased by 50%, and the low temperature resistance is increased by 5-6DEG C. The new strain of Crassostrea sikamea obtained by the use of the method can overcome the winter death problem of Crassostrea sikamea in the North and ensure the sustainable development of Crassostrea sikamea farmingindustry.
Owner:OCEAN UNIV OF CHINA

Ostrea gigas tnunb shell opening device

The invention discloses an ostrea gigas tnunb shell opening device. The ostrea gigas tnunb shell opening device is manufactured by a steel strip; the cross section of the top end of the steel strip iselliptic; the edge of the steel strip is sharp; a handle is arranged at the tail end of the steel strip; a cavity is formed in the middle part of the steel strip; and a small steel strip is arrangedin the cavity. The ostrea gigas tnunb shell opening device disclosed by the invention is simple in structure, convenient and practical; ostrea gigas tnunb shells can be unclenched without too much physical strength; and the ostrea gigas tnunb shell opening device is also suitable for other shellfish which are difficult to unshell.
Owner:GUIGANG HOUSHUN INFORMATION TECH CO LTD

A method for inducing tetraploids of a new variety of long oyster "Haida No. 2"

The invention relates to an induction method for a 'Haida 2#' new variety tetraploid of crassostrea gigas. The induction method comprises the steps of selecting a 'Haida 2#' new variety of crassostreagigas as parent oysters, carrying out manual maturity-promoting culture until the parent oysters are mature, selecting female and male individuals with good gonad development as parent oysters, dissecting for fetching ova, filtering, putting the ova into filtered seawater for maturation, enabling the maturated ova to be fertilized with sperms in the filtered seawater, processing fertilized ova byvirtue of cytochalasin B so as to inhibit the discharging of first polar bodies, after the processing, collecting the fertilized ova, transferring the fertilized ova into an ethanol solution for soaking, finally transferring the fertilized ova into a culture container, and carrying out incubation and larva culture. According to the induction method, the discharging of the first polar bodies of the fertilized ova of the ''Haida 2#'' new variety of a diploid is inhibited by virtue of the cytochalasin B, and the living 'Haida 2#' new variety tetraploids of crassostrea gigas are directly induced,so that the survival rate is greatly increased, and a seedling culturing foundation is provided for the amplified breeding of the 'Haida 2#' new variety tetraploid of crassostrea gigas.
Owner:OCEAN UNIV OF CHINA
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