7 results about "Ostrea gigas" patented technology

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The present application belongs to the technical field of aquaculture, and particularly relates to a method for improving survival rate of Dendostrea folium shell top larvae by dry exposure treatment. In the present application, the Dendostrea folium larvae in the late shell top stage are subjected to dry exposure stress treatment, and then rehydrated, fed and cultivated. Compared with the control group of oyster larvae without dry exposure stress treatment, the survival rate of the larvae cultivated by the shell top larvae subjected to dry exposure stress treatment is significantly improved under high temperature, low temperature and low oxygen stress. The present application can significantly improve the environmental stress resistance of Dendostrea folium larvae, and has a good application prospect in shellfish seed production.

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

This invention belongs to the field of molecular genetics and shellfish genome engineering, and particularly relates to a safe harbor region of the oyster genome for stable integration of exogenous genes and a screening method. The safe harbor region is a region for stable integration and expression of exogenous genes, located in different chromosomal regions of the oyster genome, in a non-open chromatin state, and meeting the following conditions: (1) the region is located on chromosomes and scaffolds, and has no coding genes / lncRNAs; (2) the region has no ATAC-seq signal; (3) the region length is 5.25–14.84 kb; (4) the average methylation level of the region is 0–0.161765; (5) the PAM (NGG) density of the region is 38.68–81.35 PAM / kb. This invention provides a reproducible and scalable general technical platform for the stable expression of oyster gene knock-in fragments and the study of their gene function.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the quality of gametes of Crassostrea gigas. Gonad undeveloped parent oysters of Crassostrea gigas are selected, and the parent oysters are treated with lipopolysaccharide, and then the gametes generated by the parent oysters are used to cultivate offspring after temperature promotion. The diameter of the gametes generated by the parent oysters of Crassostrea gigas treated with lipopolysaccharide is increased, the total antioxidant capacity is improved, and the growth speed and the attachment metamorphosis capacity of the offspring larvae are significantly improved. The application opens up a new way for solving the bottleneck problem of high mortality of larvae in the process of artificial breeding of Crassostrea gigas.

The application firstly adopts selection breeding technology combined with ploidy breeding technology to construct and cultivate long oyster tetraploid population and Fujian oyster tetraploid population which are genetically stable, fast in growth speed and high in survival rate; constructs hybrid oyster tetraploid population (GGAA) through hybridization of long oyster tetraploid and Fujian oyster tetraploid; hybridizes near Jiang oyster diploid breeding population as a female parent and GGAA as a male parent to obtain "near Jiang oyster-long oyster-Fujian oyster" three-way hybrid triploid oyster (RGA). The cultivated three-way hybrid triploid oyster can keep fast growth, high survival rate and high stress resistance under the condition of low salt sea area (<20), and at the same time, shows a relatively high proportion of infertile individuals in the breeding season. The application is suitable for large-scale preparation of three-way hybrid triploid oyster seedlings, provides matching breeding technology of three-way hybrid triploid oyster suitable for estuary, gulf and other medium and low salinity breeding sea areas of oyster industry, and has good industrial application value.