46 results about "Ostrea gigas" patented technology

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The application belongs to the field of genetic engineering and genetic breeding, and particularly relates to a high-temperature response gene HSP70 expression regulation SNP marker of Crassostrea gigas and application of the SNP marker in identifying high-temperature resistant oyster individuals. The SNP marker is the 251th base in the sequence shown in SEQ ID NO: 2, and the mutation type is C / G (trans-regulation site Marker13973). The application provides a SNP marker which is significantly related to the expression of the HSP70 gene, and the advantage of the application is that the genotype of parent oysters can be identified before seed breeding, and the tolerance of offspring to high-temperature environment is improved. The SNP marker obtained in the research has high reliability and stable results.

The present invention belongs to the field of molecular biology technology, and in particular relates to a recombinant protein of the CgVg functional domain DUF1943 and VWD of the long oyster, as well as a preparation method and application thereof. The amino acid sequence of the recombinant protein of the CgVg functional domain DUF1943 of the long oyster is shown in SEQ ID NO.1. The amino acid sequence of the recombinant protein of the CgVg functional domain VWD of the long oyster is shown in SEQ ID NO.2. The recombinant proteins of the CgVg functional domain DUF1943 and VWD of the long oyster obtained by the present invention have a wide range of binding activity against microorganisms, among which the DUF1943 recombinant protein has the activity of agglutinating bacteria and fungi, and the VWD recombinant protein has a broad spectrum of activity in inhibiting bacterial growth, and can be applied to the research and development of antibacterial drugs, immunopotentiators and feed additives for marine organisms.

The invention provides a seed production method of a new purple golden crassostrea gigas strain with a high meat yield, and belongs to the technical field of aquaculture. The method comprises the following steps: firstly, preliminarily screening out a class group with two shells which are purple golden and purple mainly, 1-3 golden shells and high meat yield as a core group; the method comprises the following steps: selecting crassostrea gigas individuals with alternately purple and golden shells and high meat percentage as an optimal population by adopting a head cutting selection method, taking the optimal population as a breeding population, and carrying out filial generation breeding according to a conventional shellfish breeding method to obtain a new purple and golden shell strain F1 with high meat percentage; further purifying the purple golden shell character in a family breeding mode to form a second-generation breeding family F2; and then a plurality of generations of continuous purification are carried out to obtain the new crassostrea gigas strain with purple gold color, high meat yield and stable ovum shape. The survival rate, the growth speed and the fatness degree of the new purple golden crassostrea gigas strain with the high meat percentage are greatly improved.

The invention discloses an oyster-derived polypeptide and application thereof, and belongs to the technical field of bioactive peptides. The oyster source polypeptide provided by the invention is selected from at least one of the following polypeptide sequences: PAPLWQMPKFRN, NPVDPAPLWQMPK, PFPL, VPPF, PLNF, APAPAVKYLR, PPMIFKTR, PPMIFKTRIM, AVNMVPFPR, FSLP, PFKP, FPSIVGRPR, VPKPYLNHPL, LPPL, PQDVPPKKPLIMIAAR, VPFVPYLNHPL, FLLK, SPPELPDVM and MPVTDPPLRTF. The invention also discloses a preparation method of the oyster source polypeptide. The invention also discloses application of the oyster-derived polypeptide in preparation of food, health care products or medicines with an immune regulation function. The oyster source peptide product disclosed by the invention has digestion stability and immunocompetence, can keep a basic structure after simulating gastric juice digestion, and can activate the immune function of RAW264.7 cells and improve the expression quantity of cell factors. The method provided by the invention can provide a new direction for high-value utilization of marine resources, and has a good application prospect.

The application belongs to the technical field of molecular biology, and particularly relates to a long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein and application. The amino acid sequence of the long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein is shown as SEQ ID NO. 1. The rCRD obtained by the application has binding capacity to various pathogen-associated molecular patterns (PAMPs), can bind and agglutinate various microorganisms, has a growth inhibiting effect on gram-negative bacteria Vibrio splendidus and Escherichia coli, and can be used for the development of bacteriostatic feed additives and new-type immune enhancers.

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The invention relates to a pair of PCR (polymerase chain reaction) primers and application thereof in identification of crassostrea gigas, Fujian oysters and hybrids thereof, and belongs to the field of molecular biology, sequences of the primers are shown as SEQ ID NO.1-2, and the invention also provides application of the primers in identification of crassostrea gigas, Fujian oysters and hybrids thereof. According to the method, the crassostrea gigas, the Fujian oyster and the hybrid thereof can be identified, and the method is simple and convenient.

The present invention discloses an application of a variant site combination, an application of a molecular marker combination, a primer combination and its application, a detection product, and a method for identifying long oysters, Fujian oysters, and hybrid oysters of the two, relating to the field of biotechnology. The method comprises the following steps: extracting genomic DNA from the individual to be tested; using the genomic DNA as a template, performing multiplex PCR amplification using a primer combination, and sequencing the PCR products; determining the species of the individual to be tested based on the base type of the SNV variant site; the primer combination comprises the primer pairs described in (c1) and (c2): (c1) a primer pair having a nucleotide sequence as shown in SEQ ID NO.1-2 and / or a primer pair having a nucleotide sequence as shown in SEQ ID NO.3-4; (c2) a primer pair having a nucleotide sequence as shown in SEQ ID NO.5-6. The method can effectively distinguish long oysters, Fujian oysters, and hybrid oysters of the two.

The application belongs to the technical field of microorganisms, and particularly relates to a long oyster parabacillus ZH-28 and application thereof. Metabacillus crassostreae The long oyster parabacillus ZH-28 has been preserved in the China General Microbiological Culture Collection Center on January 27, 2026, and the preservation number is CGMCC No.37550. Metabacillus crassostreae The long oyster parabacillus ZH-28 has strong salt and alkali resistance, and has multiple functions such as nitrogen fixation, protein degradation, cellulose degradation, fat degradation and iron carrier production, and has significant growth promotion on wheat and cucumber in a saline-alkali environment, and has a good application prospect in plant growth promotion in a saline-alkali soil.

The present application belongs to the field of aquatic animal genetics and breeding, and particularly relates to a breeding method of a new line of palm-shaped and high-meat-yield long oyster. The method comprises the following steps: (1) determining the genetic distance of each population of long oysters from different geographical sources; (2) obtaining the variation coefficient of the shell length and meat yield of long oysters; (3) calculating the genetic force of the shell length and meat yield of long oysters, and the genetic correlation between the two indexes; (4) calculating the family breeding value of the two traits, setting the weight value of the shell length and meat yield respectively, calculating the comprehensive breeding value, and screening the optimal family in proportion; (5) pairing the families among populations to improve the genetic diversity of the offspring; (6) taking the shell length and meat yield as the breeding target, and retaining the seed oysters according to the set seed retention rate every generation, the number of female and male seed oysters is maintained above the set number, and the breeding population retains the matching line.

The invention discloses an SNP marker related to oyster ERK phosphorylation level and high-temperature resistance and application of the SNP marker, and belongs to the technical field of genetic engineering and genetic breeding. The nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1, and A / G mutation exists at the 1188th site of the sequence. The ERK phosphorylation level and high-temperature resistance of an oyster individual of which the mutation site genotype of the SNP marker is GG are obviously higher than those of an oyster individual of AA and AG. Based on the molecular basis that the ERK phosphorylation level of crassostrea gigas and the ERK phosphorylation level of Fujian crassostrea gigas are remarkably different under the condition of heat stress, the key SNP marker for regulating ERK differential heat activation and the candidate gene of the key SNP marker are successfully screened out by systematically analyzing the upstream regulation pathway and combining whole genome association analysis. The marker has stable hereditary characteristics, can realize high-throughput and no-damage genetic typing of high-temperature-resistant oysters, and can be directly applied to assisted breeding.

The present application belongs to the technical field of aquaculture, and particularly relates to a method for improving survival rate of Dendostrea folium shell top larvae by dry exposure treatment. In the present application, the Dendostrea folium larvae in the late shell top stage are subjected to dry exposure stress treatment, and then rehydrated, fed and cultivated. Compared with the control group of oyster larvae without dry exposure stress treatment, the survival rate of the larvae cultivated by the shell top larvae subjected to dry exposure stress treatment is significantly improved under high temperature, low temperature and low oxygen stress. The present application can significantly improve the environmental stress resistance of Dendostrea folium larvae, and has a good application prospect in shellfish seed production.

The invention relates to the technical field of bioengineering, in particular to crassostrea gigas FMO1 protein as well as a preparation method and application thereof. The invention provides a method for efficiently preparing crassostrea gigas FMO1 protein. According to the invention, identification of a key step of crassostrea gigas taurine synthesis, namely a key taurine metabolism gene FMO1 sequence and function generated by oxidation of sub-taurine, is realized for the first time. Therefore, the Crassostrea gigas FMO1 candidate gene sequence is accurately identified, and meanwhile, the Crassostrea gigas FMO1 in-vitro recombinant protein is efficiently and massively prepared. The invention also provides a method for detecting the activity of the crassostrea gigas FMO1 in-vitro recombinant protein for catalyzing the oxidation reaction of the hypotaurine. According to the method, the activity of the FMO1 protein and the generation of the taurine can be evaluated by monitoring the consumption of NADPH (Nicotinamide Adenine Dinucleotide Phosphate) in a reaction system.

The application constructs a hybrid basic population GA F0 by group hybridization of long oyster as female parent and Fujian oyster as male parent; the GA F0 is used as a basis, shell height selection is combined with high-temperature heat stress screening, continuous multi-generation population breeding is carried out, and the GA breeding population GA F6 with rapid growth and high-temperature tolerance is obtained; the RGA F0 is constructed by hybridization of near Jiang oyster as female parent and GA F6 as male parent; the RGA F0 is used as a basic population, shell height selection is combined with low-salt and high-temperature combined stress screening, continuous multi-generation population breeding is carried out, and the fast-growing, low-salt and high-temperature-adaptable oyster three-way hybrid new strain is obtained. The application establishes an oyster three-way hybrid breeding procedure, so that the breeding population has the characteristics of rapid growth, low-salt tolerance and high-temperature tolerance, and has a good industrial application prospect.

The invention discloses a traditional Chinese medicine prescription for treating nodules and application thereof. The traditional Chinese medicine prescription comprises the following raw material medicines: selfheal, bupleurum chinense, rhizoma pinellinae praeparata, thunberg fritillary bulb, radix scrophulariae, oyster, curcuma zedoary, spina gleditsiae, turtle shell and astragalus membranaceus. According to the traditional Chinese medicine prescription disclosed by the invention, the characteristics of the raw material medicines are utilized, the formula is reasonable, the proportioning is scientific, and the synergistic efficacy is achieved. The traditional Chinese medicine prescription disclosed by the invention takes'qi regulating, phlegm reducing, blood circulation promoting, blood stasis removing, hardness softening and stagnation dissipating 'as a treatment principle, and has a good effect on treating various nodules.

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

The invention relates to the technical field of aquaculture molecular breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to a crassostrea gigas shell shape and application thereof. The SNP (Single Nucleotide Polymorphism) molecular marker obtained by screening disclosed by the invention is as follows: (1) Cg05009-24308790Cgt; t is located at the 24308790th site of the reference genome; (2), Cg05270 to 28618069Agt, Cg05270 to T is located at the 28618069th site of the reference genome; (3) Cg < 18426-31151905 > A < gt >; t is located at the 31151905th site of the reference genome. The SNP molecular markers obtained by screening provide a powerful molecular tool for developing a new oyster variety with a nearly circular shell, so that a targeted breeding plan is supported.

This invention discloses a method for identifying the parentage of tetraploid Pacific oysters based on SNP loci, belonging to the field of molecular-assisted breeding technology for tetraploid Pacific oysters. The method includes the following steps: (1) sequencing the adductor muscle of the tetraploid Pacific oyster to be tested; (2) comparing the genotype of the designated SNP locus in the tetraploid Pacific oyster with the reference genome; (3) calculating the IBS and LOD of all tested parents and offspring based on the SNP typing results; and (4) combining the IBS and LOD to identify the parentage of the tested parents and offspring. The advantages of this invention are: it discloses for the first time 1000 SNP loci for identifying the parentage of tetraploid Pacific oysters, providing data support for the identification of parentage in tetraploid Pacific oysters and laying the foundation for the development of liquid-phase chips for identifying the parentage of tetraploid Pacific oysters, showing good application prospects.

The invention discloses a traditional Chinese medicine composition for treating infertility, which is prepared from the following raw materials: radix rehmanniae recen, radix paeoniae alba, turtle shell, cortex moutan, peach kernel, ginseng, radix glehniae, medlar, oyster, rhizoma cyperi, radix curcumae, astragalus membranaceus, angelica sinensis, rhizoma anemarrhenae, golden cypress, lotus seed, rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, chicken's gizzard-membrane, radix bupleuri, tetrapanax papyriferus, donkey-hide gelatin, eucommia ulmoides and deer glue. Spleen tonifying, blood nourishing, phlegm reducing and dampness clearing; the effects of soothing liver-qi stagnation, promoting qi and activating blood are achieved; qi is benefited, blood is nourished, and Zhongzhou is promoted to regenerate Qi and blood; promoting blood circulation to remove blood stasis and dredging Chong and Ren qi; the traditional Chinese medicine composition has the effects of clearing heat, promoting diuresis, eliminating dampness and reducing phlegm, can achieve sufficient qi and blood of liver and kidney and smooth Chong and Ren channels, has a good treatment effect on male and female infertility caused by various reasons, and is particularly suitable for female infertility caused by kidney deficiency, weakness, fallopian tube blockage, cold uterus, kidney deficiency type ovulation disorder, obesity, stasis, weakness, blood deficiency and the like; and the traditional Chinese medicine composition is also suitable for male infertility caused by cold sperm, rare sperm, oligospermia and necrospermia due to kidney deficiency.

The present invention relates to a method for screening long oyster broodstock with high glycogen content and its related SNP primer pairs. Through whole-genome association analysis of 486 individuals, 67 SNP signals significantly correlated with glycogen content on chromosome 6 of long oysters were identified. A corresponding detection method was developed for a SNP site at the scaffold1243_53489 base in this region, and the results showed that the SNP site can be detected simply and quickly using this method. At the same time, individuals with the CC genotype at this site are compared with individuals with the TT genotype. Parent oysters with the CC genotype are screened to guide oyster breeding. The nucleotide sequence of the long oyster 500kb upstream and downstream of this site is shown in SEQ ID No.1. The beneficial effect of the present invention is to identify the genotype of parent oysters before seed breeding, thereby increasing the glycogen content of the offspring. The SNP markers obtained by this study are more reliable and have more stable effects.

The invention discloses a standardized evaluation method for shell hardness of crassostrea gigas, and belongs to the field of aquatic shellfish mechanical phenotype determination and biomineralization material performance detection. The method comprises the following steps: carrying out cleaning pretreatment on double shells of crassostrea gigas; respectively determining the longest axis and the widest axis in the contours of the left shell and the right shell, constructing an orthogonal reference cross axis, axially arranging three standardized measurement sites of the left shell and the right shell at positions about 5mm away from the inner sides of the edges of the shells, and marking the standardized measurement sites; cutting the marked area to obtain a flat shell sample; applying a load of about 10 gf to each measurement site by using a Vickers microhardness tester, maintaining the pressure for about 10 seconds, and carrying out multiple indentation tests to obtain an original hardness value; carrying out abnormal value elimination and mean value calculation on the data to obtain representative hardness values of all sites; and further comprehensively calculating representative hardness values of six sites of the left shell and the right shell, and constructing an individual comprehensive shell hardness index. Compared with an existing single-point or random sampling measurement method, the method has the advantages that the problems of irregular shell shapes, asymmetry of left shells and right shells and the like of oysters are considered, and the accuracy and repeatability of oyster hardness phenotype measurement are remarkably improved through standardization of measurement sites and multi-site high-repetition measurement. The method can be widely applied to the fields of oyster stress resistance evaluation, marine acidification, environmental monitoring and the like, provides possibility for accurate phenotype determination in GWAS and QTL positioning in breeding, and has a huge application prospect.

The invention discloses application of crassostrea gigas miR-182-5p in negative regulation of melanin synthesis, and belongs to the technical field of aquatic genetic breeding and biology. The miR-182-5p in the crassostrea gigas is interfered by the miR-182-5p inhibitor, synthesis of melanin of the crassostrea gigas is remarkably enhanced after the miR-182-5p is interfered, and the color of the crassostrea gigas is black. In addition, the invention also synthesizes an exclusive miR-182-5p stimulant to treat the crassostrea gigas. Results show that compared with wild crassostrea gigas, the content of melanin in the crassostrea gigas injected with the simulant is lower, and the shell color is white. Therefore, the miR-182-5p is in negative correlation with the synthesis of the melanin of the crassostrea gigas, so that the miR-182-5p not only can be used for selecting or predicting the shell color character of the crassostrea gigas, but also can be used for assisting in screening or culturing varieties with high melanin content by silencing or knocking out the miR-182-5p in the crassostrea gigas. The important effect of miR-182-5p in oyster melanin synthesis is clarified for the first time, the molecular mechanism of mollusk melanin formation can be deeply understood, and the miR-182-5p has important significance in promoting practical application of crassostrea gigas quality breeding.

The present application relates to the technical field of beverage preparation, and discloses a kudzu root and oyster peptide beverage preparation device, which comprises a main body, a fixed frame is fixedly connected to the outer surface of the main body, a hot air pump is fixedly connected to the top of the fixed frame, a liquid inlet pipe is fixedly connected to the top of the main body, one end of the liquid inlet pipe close to the fixed frame penetrates through the top inner wall of the main body and extends to the outside, and a partition plate is fixedly connected to the inside of the main body. When the concentrated liquid is sprayed in the form of mist, the mist-shaped concentrated liquid will fall into the middle of the hollow disc, and then part of the mist-shaped concentrated liquid will be rolled into the vortex along with the flow of the vortex when the vortex is generated and flows, at this time, the hot air can fully contact the mist-shaped concentrated liquid when the vortex generated by the hot air flows, and the mist-shaped concentrated liquid can be quickly taken away, and meanwhile, the vortex can form strong disturbance to the concentrated liquid, so that the heat exchange between the mist droplets and the hot air is more sufficient, thereby shortening the drying time and improving the drying efficiency.

The invention discloses a crassostrea gigas culture site selection method, device, equipment, medium and product, and relates to the field of aquaculture site selection, and the method comprises the steps: obtaining the actual multi-source marine environment data of crassostrea gigas, and carrying out the prediction through a global scale model according to a global scale marine environment variable, and obtaining the global scale species existence probability; the global scale model comprises a plurality of integrated global scale crassostrea gigas ecological niche single models; taking the regional scale marine environment variable as the input of the nested ecological niche distribution model, taking the global scale species existence probability as the covariable of the nested ecological niche distribution model, and processing based on a covariable strategy to obtain the final species existence probability; the nested ecological niche distribution model is an integrated multi-regional-scale crassostrea gigas ecological niche single model; and according to the final species existence probability, carrying out suitability evaluation to obtain an evaluation result. According to the invention, the reliability of site selection can be improved.

The invention provides a method for producing tetraploid oysters with different shell colors through interspecific hybridization. The method comprises the following steps: firstly, selecting Fujian oysters and crassostrea gigas with common shell color, golden shell color and black shell color and excellent growth traits as parents, and respectively obtaining triploid and tetraploid individuals through a chemical induction technology; and then carrying out continuous 2-4 generations of population breeding to obtain Fujian oyster and crassostrea gigas tetraploid populations with stable ploidy and different shell color characteristics. Then, the tetraploid populations are hybridized to obtain hybrid tetraploid oysters with different shell colors, and the hybrid tetraploid oysters have three shell colors including a common shell color, a gold shell color and a black shell color. The produced tetraploid oysters have high growth speed, high ploidy stability and high survival rate, and excellent germplasm resources can be provided for the triploid oyster industry. The method not only improves the cultivation benefits of the oysters, but also has wide industrial application prospects. The method combines the technologies of selective breeding, polyploid breeding, cross breeding and the like, breaks through the limitation of a traditional breeding method, has higher breeding efficiency and application potential, is particularly suitable for oyster breeding in coastal areas of China, and promotes the oyster industry to be transformed and upgraded to a high-end consumer market.

The invention discloses a recombinant crassostrea gigas complement C3 cleavage fragment rCgC3dg as well as a preparation method and application thereof. The amino acid sequence of the rCgC3dg is shown as SEQ ID NO. 1; the preparation method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a Crassostrea gigas CgC3dg coding region fragment by using primers P1 and P2, wherein the DNA (Deoxyribose Nucleic Acid) sequence of the primer P1 is as shown in SEQ ID NO. 2, and the DNA sequence of the primer P2 is as shown in SEQ ID NO. 3; respectively carrying out BamHI and Xhol I enzyme digestion on a PCR amplification product and a pET-30a carrier, and then connecting and transforming through T4 ligase to construct a recombinant; transferring the recombinant into an escherichia coli Transsetta (DE3) expression strain, culturing the expression strain, adding IPTG (isopropyl-beta-d-thiogalactoside) to carry out induced expression on the recombinant protein, and then purifying and renaturating to obtain the recombinant rCgC3dg protein. The recombinant crassostrea gigas complement C3 split fragment rCgC3dg and rCgGSDME-N can be used for preparing a medicine for resisting vibrio splendidus and escherichia coli after being mixed.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the attachment rate and disease resistance of long oyster (Crassostrea gigas) nauplii and application thereof. The method is that early nauplii of the long oyster are soaked in filtered seawater containing a certain dose of glutathione. Compared with a control group without adding glutathione, the growth and development speed of the long oyster nauplii soaked in the glutathione is accelerated, the attachment rate is significantly improved, the lysozyme activity is enhanced, and the ability to resist vibrio is significantly increased. The application has a significant effect on reducing the mortality of the long oyster nauplii in the attachment metamorphosis process and improving the attachment metamorphosis rate, and can be used as an immune enhancer for the attachment metamorphosis stage of the long oyster and applied to seed production.

The application discloses a method for obtaining and preserving tetraploid male Crassostrea gigas, and belongs to the technical field of genetic breeding of aquatic breeding varieties. The method comprises the following steps: accelerating the maturity of tetraploid Crassostrea gigas by regulating water temperature and bait, identifying the male and female of the tetraploid Crassostrea gigas by nuclear magnetic resonance technology, further accelerating the maturity of the tetraploid male Crassostrea gigas by regulating water temperature and bait, and preserving the tetraploid male Crassostrea gigas by well storage seawater. The method has the advantages of simple operation, no influence of external air temperature and seawater temperature, no damage to the tetraploid Crassostrea gigas, and can provide the tetraploid male Crassostrea gigas stably throughout the year, thereby laying a solid foundation for the stable cultivation of triploid Crassostrea gigas throughout the year.

This invention belongs to the field of molecular genetics and shellfish genome engineering, and particularly relates to a safe harbor region of the oyster genome for stable integration of exogenous genes and a screening method. The safe harbor region is a region for stable integration and expression of exogenous genes, located in different chromosomal regions of the oyster genome, in a non-open chromatin state, and meeting the following conditions: (1) the region is located on chromosomes and scaffolds, and has no coding genes / lncRNAs; (2) the region has no ATAC-seq signal; (3) the region length is 5.25–14.84 kb; (4) the average methylation level of the region is 0–0.161765; (5) the PAM (NGG) density of the region is 38.68–81.35 PAM / kb. This invention provides a reproducible and scalable general technical platform for the stable expression of oyster gene knock-in fragments and the study of their gene function.