20 results about "Ostrea gigas" patented technology

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The application belongs to the technical field of microorganisms, and particularly relates to a long oyster parabacillus ZH-28 and application thereof. Metabacillus crassostreae The long oyster parabacillus ZH-28 has been preserved in the China General Microbiological Culture Collection Center on January 27, 2026, and the preservation number is CGMCC No.37550. Metabacillus crassostreae The long oyster parabacillus ZH-28 has strong salt and alkali resistance, and has multiple functions such as nitrogen fixation, protein degradation, cellulose degradation, fat degradation and iron carrier production, and has significant growth promotion on wheat and cucumber in a saline-alkali environment, and has a good application prospect in plant growth promotion in a saline-alkali soil.

The invention discloses an SNP marker related to oyster ERK phosphorylation level and high-temperature resistance and application of the SNP marker, and belongs to the technical field of genetic engineering and genetic breeding. The nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1, and A / G mutation exists at the 1188th site of the sequence. The ERK phosphorylation level and high-temperature resistance of an oyster individual of which the mutation site genotype of the SNP marker is GG are obviously higher than those of an oyster individual of AA and AG. Based on the molecular basis that the ERK phosphorylation level of crassostrea gigas and the ERK phosphorylation level of Fujian crassostrea gigas are remarkably different under the condition of heat stress, the key SNP marker for regulating ERK differential heat activation and the candidate gene of the key SNP marker are successfully screened out by systematically analyzing the upstream regulation pathway and combining whole genome association analysis. The marker has stable hereditary characteristics, can realize high-throughput and no-damage genetic typing of high-temperature-resistant oysters, and can be directly applied to assisted breeding.

The present application belongs to the technical field of aquaculture, and particularly relates to a method for improving survival rate of Dendostrea folium shell top larvae by dry exposure treatment. In the present application, the Dendostrea folium larvae in the late shell top stage are subjected to dry exposure stress treatment, and then rehydrated, fed and cultivated. Compared with the control group of oyster larvae without dry exposure stress treatment, the survival rate of the larvae cultivated by the shell top larvae subjected to dry exposure stress treatment is significantly improved under high temperature, low temperature and low oxygen stress. The present application can significantly improve the environmental stress resistance of Dendostrea folium larvae, and has a good application prospect in shellfish seed production.

The invention relates to the technical field of bioengineering, in particular to crassostrea gigas FMO1 protein as well as a preparation method and application thereof. The invention provides a method for efficiently preparing crassostrea gigas FMO1 protein. According to the invention, identification of a key step of crassostrea gigas taurine synthesis, namely a key taurine metabolism gene FMO1 sequence and function generated by oxidation of sub-taurine, is realized for the first time. Therefore, the Crassostrea gigas FMO1 candidate gene sequence is accurately identified, and meanwhile, the Crassostrea gigas FMO1 in-vitro recombinant protein is efficiently and massively prepared. The invention also provides a method for detecting the activity of the crassostrea gigas FMO1 in-vitro recombinant protein for catalyzing the oxidation reaction of the hypotaurine. According to the method, the activity of the FMO1 protein and the generation of the taurine can be evaluated by monitoring the consumption of NADPH (Nicotinamide Adenine Dinucleotide Phosphate) in a reaction system.

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

This invention discloses a method for identifying the parentage of tetraploid Pacific oysters based on SNP loci, belonging to the field of molecular-assisted breeding technology for tetraploid Pacific oysters. The method includes the following steps: (1) sequencing the adductor muscle of the tetraploid Pacific oyster to be tested; (2) comparing the genotype of the designated SNP locus in the tetraploid Pacific oyster with the reference genome; (3) calculating the IBS and LOD of all tested parents and offspring based on the SNP typing results; and (4) combining the IBS and LOD to identify the parentage of the tested parents and offspring. The advantages of this invention are: it discloses for the first time 1000 SNP loci for identifying the parentage of tetraploid Pacific oysters, providing data support for the identification of parentage in tetraploid Pacific oysters and laying the foundation for the development of liquid-phase chips for identifying the parentage of tetraploid Pacific oysters, showing good application prospects.

The invention discloses a traditional Chinese medicine composition for treating infertility, which is prepared from the following raw materials: radix rehmanniae recen, radix paeoniae alba, turtle shell, cortex moutan, peach kernel, ginseng, radix glehniae, medlar, oyster, rhizoma cyperi, radix curcumae, astragalus membranaceus, angelica sinensis, rhizoma anemarrhenae, golden cypress, lotus seed, rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, chicken's gizzard-membrane, radix bupleuri, tetrapanax papyriferus, donkey-hide gelatin, eucommia ulmoides and deer glue. Spleen tonifying, blood nourishing, phlegm reducing and dampness clearing; the effects of soothing liver-qi stagnation, promoting qi and activating blood are achieved; qi is benefited, blood is nourished, and Zhongzhou is promoted to regenerate Qi and blood; promoting blood circulation to remove blood stasis and dredging Chong and Ren qi; the traditional Chinese medicine composition has the effects of clearing heat, promoting diuresis, eliminating dampness and reducing phlegm, can achieve sufficient qi and blood of liver and kidney and smooth Chong and Ren channels, has a good treatment effect on male and female infertility caused by various reasons, and is particularly suitable for female infertility caused by kidney deficiency, weakness, fallopian tube blockage, cold uterus, kidney deficiency type ovulation disorder, obesity, stasis, weakness, blood deficiency and the like; and the traditional Chinese medicine composition is also suitable for male infertility caused by cold sperm, rare sperm, oligospermia and necrospermia due to kidney deficiency.

The invention discloses a standardized evaluation method for shell hardness of crassostrea gigas, and belongs to the field of aquatic shellfish mechanical phenotype determination and biomineralization material performance detection. The method comprises the following steps: carrying out cleaning pretreatment on double shells of crassostrea gigas; respectively determining the longest axis and the widest axis in the contours of the left shell and the right shell, constructing an orthogonal reference cross axis, axially arranging three standardized measurement sites of the left shell and the right shell at positions about 5mm away from the inner sides of the edges of the shells, and marking the standardized measurement sites; cutting the marked area to obtain a flat shell sample; applying a load of about 10 gf to each measurement site by using a Vickers microhardness tester, maintaining the pressure for about 10 seconds, and carrying out multiple indentation tests to obtain an original hardness value; carrying out abnormal value elimination and mean value calculation on the data to obtain representative hardness values of all sites; and further comprehensively calculating representative hardness values of six sites of the left shell and the right shell, and constructing an individual comprehensive shell hardness index. Compared with an existing single-point or random sampling measurement method, the method has the advantages that the problems of irregular shell shapes, asymmetry of left shells and right shells and the like of oysters are considered, and the accuracy and repeatability of oyster hardness phenotype measurement are remarkably improved through standardization of measurement sites and multi-site high-repetition measurement. The method can be widely applied to the fields of oyster stress resistance evaluation, marine acidification, environmental monitoring and the like, provides possibility for accurate phenotype determination in GWAS and QTL positioning in breeding, and has a huge application prospect.

The invention discloses application of crassostrea gigas miR-182-5p in negative regulation of melanin synthesis, and belongs to the technical field of aquatic genetic breeding and biology. The miR-182-5p in the crassostrea gigas is interfered by the miR-182-5p inhibitor, synthesis of melanin of the crassostrea gigas is remarkably enhanced after the miR-182-5p is interfered, and the color of the crassostrea gigas is black. In addition, the invention also synthesizes an exclusive miR-182-5p stimulant to treat the crassostrea gigas. Results show that compared with wild crassostrea gigas, the content of melanin in the crassostrea gigas injected with the simulant is lower, and the shell color is white. Therefore, the miR-182-5p is in negative correlation with the synthesis of the melanin of the crassostrea gigas, so that the miR-182-5p not only can be used for selecting or predicting the shell color character of the crassostrea gigas, but also can be used for assisting in screening or culturing varieties with high melanin content by silencing or knocking out the miR-182-5p in the crassostrea gigas. The important effect of miR-182-5p in oyster melanin synthesis is clarified for the first time, the molecular mechanism of mollusk melanin formation can be deeply understood, and the miR-182-5p has important significance in promoting practical application of crassostrea gigas quality breeding.

The invention discloses a crassostrea gigas culture site selection method, device, equipment, medium and product, and relates to the field of aquaculture site selection, and the method comprises the steps: obtaining the actual multi-source marine environment data of crassostrea gigas, and carrying out the prediction through a global scale model according to a global scale marine environment variable, and obtaining the global scale species existence probability; the global scale model comprises a plurality of integrated global scale crassostrea gigas ecological niche single models; taking the regional scale marine environment variable as the input of the nested ecological niche distribution model, taking the global scale species existence probability as the covariable of the nested ecological niche distribution model, and processing based on a covariable strategy to obtain the final species existence probability; the nested ecological niche distribution model is an integrated multi-regional-scale crassostrea gigas ecological niche single model; and according to the final species existence probability, carrying out suitability evaluation to obtain an evaluation result. According to the invention, the reliability of site selection can be improved.

This invention belongs to the field of molecular genetics and shellfish genome engineering, and particularly relates to a safe harbor region of the oyster genome for stable integration of exogenous genes and a screening method. The safe harbor region is a region for stable integration and expression of exogenous genes, located in different chromosomal regions of the oyster genome, in a non-open chromatin state, and meeting the following conditions: (1) the region is located on chromosomes and scaffolds, and has no coding genes / lncRNAs; (2) the region has no ATAC-seq signal; (3) the region length is 5.25–14.84 kb; (4) the average methylation level of the region is 0–0.161765; (5) the PAM (NGG) density of the region is 38.68–81.35 PAM / kb. This invention provides a reproducible and scalable general technical platform for the stable expression of oyster gene knock-in fragments and the study of their gene function.

The invention discloses an ascorbic acid-based oyster umami peptide browning targeted inhibition and nutritional ingredient synergistic preservation method, and relates to the technical field of aquatic product processing, and the method comprises the following steps: selecting fresh crassostrea gigas adductor muscle, washing with sterile water, carrying out standing pretreatment, and crushing and pulping by adopting a grinding technology to obtain oyster primary pulp; adding an ascorbic acid solution into the oyster primary pulp, stirring and mixing under a dark condition, and balancing for a preset time, so as to construct a browning substrate targeted binding system, and the like. According to the invention, through targeted combination of ascorbic acid and a browning substrate, phenolic substances, free amino acids and other components which are easy to cause browning are locked in advance; an ascorbic acid-composite antioxidant synergistic effect mechanism is formed by combining with a composite antioxidant composed of a green tea extract and citric acid, the problems that a traditional single anti-browning means is limited in effect and prone to being affected by the environment and losing efficacy are effectively solved, the browning risk of the whole processing flow of the oyster flavor peptide is reduced, and the natural color and luster of the product are reserved to the maximum extent.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 0.5 part of radix saposhnikoviae, 0.5 part of dandelion, 0.5 part of honeysuckle flower, 0.5 part of prepared kusnezoff monkshood root, 0.5 part of radix angelicae pubescentis, 0.5 part of angelica sinensis, 0.5 part of rheum officinale, 0.5 part of lindera aggregata, 0.5 part of radix sophorae flavescentis, 0.5 part of radix paeoniae rubra, 0.5 part of radix angelicae, 0.5 part of ground beeltle, 0.5 part of radix euphorbiae lantu, 0.5 part of gallnut, 0.5 part of asarum, 0.5 part of edible tulip, 0.5 part of oyster, 0.5 part of pollen typhae, 0.5 part of notopterygium root, 0.5 part of rhizoma bletillae and 0.5 part of ligusticum wallichii. The traditional Chinese medicine composition disclosed by the invention can be prepared into ointment after being decocted and taken according to the weight ratio, has the functions of relieving pain, stopping bleeding, promoting blood circulation, removing blood stasis, diminishing swelling, removing stasis and the like, and is good in fracture treatment effect, rapid in healing and high in cure rate.

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating chronic pulmonary heart disease in the heart failure stage and application of the traditional Chinese medicine composition. 15 g of ginseng; 10 g of herba ephedrae; 15 g of rhizoma belamcandae; 10 g of dried ginger; 5 g of asarum; 15 g of white mulberry root-bark; 30 g of semen lepidii; 30 g of a magnet; 30 g of oyster; 15g of poria with hostwood; 15 g of semen boitae; 15 g of polygala tenuifolia; 15g of schisandra chinensis; the traditional Chinese medicine composition is prepared by the following steps: pretreating the traditional Chinese medicine raw materials, decocting with water twice, and combining filtrate, so that the finally prepared traditional Chinese medicine composition has the effects of tonifying qi and warming yang, tonifying lung and kidney, purging lung and inducing diuresis, reducing phlegm and relieving asthma, and calming heart and tranquilizing, and is suitable for chronic pulmonary heart diseases caused by yang deficiency and water flooding, turbid phlegm obstructing lung and heart and kidney deficiency. The traditional Chinese medicine composition can obviously improve the symptoms of cough and asthma, palpitation, edema, shortness of breath, weakness and restlessness at night, and clinical verification shows that the traditional Chinese medicine composition is definite in curative effect, high in safety and suitable for clinical popularization and application.

The invention belongs to the technical field of molecular breeding of aquatic products, and relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers remarkably associated with egg diameter and brood quantity characters of crassostrea gigas and application. According to the invention, through high-throughput whole genome re-sequencing and whole genome association analysis (GWAS), a group of SNP loci closely associated with the breeding traits of crassostrea gigas are mined. Wherein the marker associated with the egg diameter character is located at the G / T variation at the 44, 274 and 019 bp of the NC047563.1 chromosome and the A / G variation at the 44, 274 and 322 bp of the NC047563.1 chromosome, and the marker associated with the egg diameter character is located at the T / A variation at the 9, 556 and 289 bp of the NC047568.1 chromosome; the marker associated with the brood quantity character is located at 25, 410 and 510 bp of the NC047564.1 chromosome C / G variation, in addition, the dominant genotypes and combination of the sites are determined, early prediction of the breeding potential of individuals and parent screening can be achieved through genotype detection in the crassostrea gigas seedling stage or non-breeding season, and the breeding potential of the individuals can be predicted. The breeding efficiency of the new high-fecundity crassostrea gigas variety is obviously improved.

The invention discloses a method for accurately evaluating the germplasm purity of crassostrea gigas, and relates to the technical field of aquatic genetic breeding biology, the core of the method is to construct an index based on an NCBI (National Center of Biotechnology Information) crassostrea gigas genome sequence by virtue of a whole genome re-sequencing technology, collect 150 crassostrea gigas and 150 Fujian oysters in a natural population, extract mantle DNA (Deoxyribose Nucleic Acid) for sequencing, screen out 635 specific SNP (Single Nucleotide Polymorphism) sites, and obtain the purity of the crassostrea gigas. The method comprises the following steps: constructing a pure variety feature sequence, sequencing a to-be-detected sample, comparing two detection sequences of 635 sites with the feature sequence, and calculating a consistency average value according to the same base number / sequence length * 100%, namely the germplasm purity value. The method fills the blank that quantitative evaluation cannot be carried out in the prior art, is high in precision and reliability, and can provide key technical support for crassostrea gigas breeding improvement, germplasm protection, industrial standardization and the like.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the quality of gametes of Crassostrea gigas. Gonad undeveloped parent oysters of Crassostrea gigas are selected, and the parent oysters are treated with lipopolysaccharide, and then the gametes generated by the parent oysters are used to cultivate offspring after temperature promotion. The diameter of the gametes generated by the parent oysters of Crassostrea gigas treated with lipopolysaccharide is increased, the total antioxidant capacity is improved, and the growth speed and the attachment metamorphosis capacity of the offspring larvae are significantly improved. The application opens up a new way for solving the bottleneck problem of high mortality of larvae in the process of artificial breeding of Crassostrea gigas.

The application firstly adopts selection breeding technology combined with ploidy breeding technology to construct and cultivate long oyster tetraploid population and Fujian oyster tetraploid population which are genetically stable, fast in growth speed and high in survival rate; constructs hybrid oyster tetraploid population (GGAA) through hybridization of long oyster tetraploid and Fujian oyster tetraploid; hybridizes near Jiang oyster diploid breeding population as a female parent and GGAA as a male parent to obtain "near Jiang oyster-long oyster-Fujian oyster" three-way hybrid triploid oyster (RGA). The cultivated three-way hybrid triploid oyster can keep fast growth, high survival rate and high stress resistance under the condition of low salt sea area (<20), and at the same time, shows a relatively high proportion of infertile individuals in the breeding season. The application is suitable for large-scale preparation of three-way hybrid triploid oyster seedlings, provides matching breeding technology of three-way hybrid triploid oyster suitable for estuary, gulf and other medium and low salinity breeding sea areas of oyster industry, and has good industrial application value.