3612 results about "Bacteroides species" patented technology

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 76 to 77 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 68 to 69 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

The invention discloses a composite microorganism viable bacteria preparation, which contains pediococcus, yeast and bacillus with the total bacteria count of more than 1 billion per gram. The invention also discloses a method for preparing the composite microorganism viable bacteria preparation, which comprises the following steps: performing amplification liquid culture on various strains respectively according to a conventional method in the field; mixing the materials according to a required proportion; and performing solid fermentation, drying and pulverization to obtain the composite microorganism viable bacteria preparation. The invention also discloses application of the composite microorganism viable bacteria preparation in preparing an organic waste water purifying agent and an aquaculture water body purifying agent, a deodorizer, a degreasing agent or an agent for removing pipe blockages for the hygiene of a refuse tip, a public place, an agricultural product market or a kitchen, feed additives in poultry and animal husbandry or pet culture, and preparations for controlling agricultural microorganism diseases. The composite microorganism viable bacteria preparation can effectively decompose a large amount of putrescent organic matters, inhibit the growth of harmful microorganisms and eliminate peculiar smells, and has the characteristics of high ammonia nitrogen removal rate, no generation of residual sludge, effective deodorization, low cost and the like.

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The invention relates to Bacteroides fragilis and applications thereof, particularly to Bacteroides fragilis ZY-312 having the preservation number of CGMCC No.10685, and applications of the Bacteroides fragilis ZY-312 in preparation of medicines, pharmaceutical compositions, foods, health products and food additives for prevention and / or treatment of antibiotic-associated diarrhea, wherein the Bacteroides fragilis ZY-312 is preserved in the China general microbiological culture collection center on April 2, 2015, has the preservation number of CGMCC No.10685, does not contain enterotoxin gene bft, has characteristics of significantly-improved cholate resistance and significantly-improved gastric acid resistance compared to the existing Bacteroides fragilis, and can effectively prevent and / or treat antibiotic-associated diarrhea.

The present invention belongs to the field of food biotechnology and is especioally microbial conversion process of preparing gamma-amino butyric acid. Gamma-amino butyric acid is prepared with lactic acid bacteria or lactic acid bacteria and saccharomycete mixture as bacteria strain, sodium L-glutamate as converting substrate and through fermentating process with fermenting medium with carbon source, nitrogen source and inorganic salt. The selected lactic acid bacteria can growth well in MRS or PYG culture medium at 25-40 deg.c and may be used as culture seed liquid. Of the culture medium, the carbon source is glucose, and the nitrogen source may be one or several selected from corn paste, yeast paste, defatted peanut cake powder, etc. After fermentation, the concentration of gamma-aminoacid may reach as high as 300-500 ml / 100 ml.

The invention relates to a convenient preparation method for culturing ecological bacteria. The product comprises a composite microbial freeze-drying strain, a special culture medium, a flora coordination and growth promotion factor and the like. After the freeze-drying microbial strains and nutrients are mixed according to the weight ratio of 1:100-1:100000, finished products are obtained by sealing and encapsulating. When in use, the product is added with water with the quantity of 20 times, the mixture is stirred evenly, the environmental temperature is controlled between 15 DEG C and 40 DEG C, and the mixture is covered by a cover, sealed at a dry position and then can be fed after being naturally activated for 2-3 days. The added water is required to be drinking water and does not contain a disinfector, a bactericide, an antibiotic and a synthetic antibacterial agent, and the temperature of the water can not exceed 45 DEG C, warm water with the temperature of 25-37 DEG C is preferred; a used container must be washed and cleaned, is provided with a cover for sealing and can be recycled by disinfection after being used. At present, most of the active bacterium products sold in the market are packaged and delivered to the market after a microbial agent and the products are fermented in a factory, which is a sale-after-growth mode; the defects of unstable effect, unreasonable performance-price ratio and the like generally exist in the active bacterium products produced by this mode. The invention adopts a growth-after-sale mode and hands the production priority of a microbial preparation to a user, and the user can prepare and culture the required ecological bacteria under a simple condition by carrying out water addition, temperature control and natural fermentation on the product. The convenient bacteria do not contain chemical medicaments and toxin and are ecological and environment-friendly.

The invention discloses an enterococcus faecium EF08 as well as a feed additive and a feed containing the enterococcus faecium EF08. The enterococcus faecium EF08 is collected with the number of CGMCC (China General Microbiological Culture Collection Center) No.5549 in CGMCC. The feed additive disclosed by the invention can be used for reducing the feed excessively consumed by harmful bacteria in intestines by virtue of an equalizing effect of bacteria for equalizing the intestinal environment, furthermore, the conversion rate of the feed for economic animals is increased, the feed for the economic animals can be effectively converted into a primary animal product with an economic value, and then, the effect of increasing the economic values of the economic animals can be improved.

The invention relates to bsacillus subtilis and microbial inoculum using the bsacillus subtilis as active ingredients, which are applied to the field of fruit freshness keeping. The bsacillus subtilis related by the invention is named as Bsacillus subtilis J18, which is preserved in China General Microbiological Culture Preservation and Management Center at present with the preservation number of CGMCC NO: 3665. Strains are prepared into fermentation agents after fermentation culture, and the fermentation agents are respectively mixed with calcium chloride and chitosan solution to be prepared into dry powder preparations and fruit glaze agents with obvious prevention and treatment effects on pear melasma after picking, black rot of pear, grape grey mould, watermelon wilt and the like. The bsacillus subtilis J18 belongs to a strain of antagonistic bacteria with wide-spectrum resistance on fruit and crop diseases.

The invention relates to a bacteria strain for short-cut denitrification and an application thereof. The short-cut denitrification bacteria strain is arthrobacter creatinolyticus FDN-1 and is preserved in Ordinary Microorganism Center of China Committee for Culture Collection of Microorganisms on March 11, 2010, and the preservation number is CGMCC No. 3657. The bacteria strain can directly adopt nitrite nitrogen as a substrate to complete the short-cut denitrification process. When the arthrobacter creatinolyticus FDN-1 is used for processing ammonia-contained waste water, the technique is simple, high denitrification activity can be still obtained under the condition that low-concentration organic carbon source exists, and at the same time the high-concentration organic carbon source can be withstood. When the bacteria strain is in use, the system is rapid to start, and a vast application prospect can be realized in the denitrification treatment process of different types of waste water.

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

The invention discloses a preparation method of a combined microbial inoculum which is suitable for being inoculated in an early farmyard manure stage and is prepared by solid inclined plane cultivation of monomer strains, propagation cultivation of seed fluids and combined fermentation fluid cultivation that uses candida utilis, lactobacillus plantarum and bacillus subtilis. The number of active microorganisms can reach over 1 multiplied by 10 per milliliter. The combined microbial inoculum can be applied into farmyard manure processing of municipal refuse or high-temperature farmyard manure processing of poultry excrement. By the inoculation in the early farmyard manure stage, the reinforcement of early-stage acidification and substrate fungus activation as well as the implementation of quick through decomposition, the farmyard manure quality is improved and the farmyard manure production efficiency is raised.

The invention relates to a kind of microbic reagent curing breed aquatics water by multi-bacteria. It can degrade and transform organic contamination of water, prohibite the growth of harmful algae and pathogeny bacteria, and promote propagation of beneficial creature, forming good entironment for growth of aquatic animal. Environment select breed and abduction select breed are adopted in this invention, spontaneous aberration and directional breed are combined, fermentated technology of original compound breed and domestication are used to make many bacteria of improved water mixed and matched each other. What' s more, growth factor and special carrier are added for promoting the growth of microbe, guaranteeing the efficiency of improvement. This product can take the place of antibiotic in feed, producing organic farm products which are green, safe and antibiotic-free.

The invention relates to a compound microecological preparation used to purify aquaculture water in the field of aquiculture, a preparation form and a preparation process thereof; the preparation is composed of photosynthetic bacterium, bacillus subtilis, denitrifying bacterium, coagulant aid and auxiliary components; the preparation form is composed of an inner layer and an outer layer, wherein, the outer layer comprises bacillus subtilis, denitrifying bacterium and coagulant aid, and the inner layer comprises solid photosynthetic bacterium particles. The invention breaks through the prior theory that people keeps using nitrite bacteria and nitrobacteria to solve the problem of the accumulation of ammonia nitrogen and nitrite in the aquaculture water in the control of aquaculture water, introduces an advanced SHARON theory in wastewater treatment, integrates the characteristics of the aquaculture water quality, purifies the aquaculture water with denitrifying bacteria strains as the main bacteria strains, can quickly and effectively reduce the content of NH3 and NO2- in the aquaculture water, is not inhibited by the concentration of the NO2-, and effectively reduces the oxygen consumption in the aquaculture water.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention discloses a lactobacillus plantarum and a preparation method thereof for high-density culture and freeze-drying bacteria powder, and has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum P8) is probiotics separated from the traditional fermentation milk product (yoghurt) of the Urat Middle Banner of Inner Mongolia Bayan Nur. The probiotics has the characteristics of artificial gastric juice and artificial digestive juice tolerance, cholate tolerance, intestinal agglutination action and common pathogenic entero bacteria inhibition. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5468 on 18th, Nov., 2011.

This invention relates to selection and application of thermophil denitriding Bacillales,of which conservation number is CGMCC-1228. Initial strain is from water of oil field. The strain is obtained by selection, inoculation, and domestication with good thermal stability. It belongs to soil Bacillales and can be used in fermented industry, which needs thermally stable enzyme. It grows well in the oil field and can degrade paraffin and decrease viscosity to improve liquidity. All of this can increase recovery and carriage efficiently. It can be used to control pollution and prepare surfactant.

The microbial fertilizer is mixture of fermented liquids separately with photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria. It is prepared through inoculating photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria separately to the first stage culture medium, and culturing to obtain the first stage bacterial seeds; inoculating the first stage bacterial seeds separately to second stage culture medium, and culturing to obtain the second stage bacterial seeds; and inoculating the second stage bacterial seeds separately to the fermenting culture medium, and culturing to obtain fermented bacterial liquids; and mixing the fermented bacterial liquids in certain proportion to obtain the microbial fertilizer. The present invention also relates to the application of the microbial fertilizer in controlling diseases of tobacco and trees and the usage.

The invention relates to a strain of Staphylococcus cohnii, and applications thereof. The strain is Staphylococcus cohnii FSDN-C, and is preserved in the China General Microbiological Culture Collection Center on July 14, 2011, wherein a preservation number is CGMCCNO.5062. According to the present invention, the strain can adopt nitrite nitrogen as a substrate to complete short time denitrification denitrogenation, wherein organic pollutants can be removed during denitrogenation; when the Staphylococcus cohnii FSDN-C is adopted to treat ammonia-containing wastewater, characteristics of simple process, and high concentration organic carbon source tolerance are provided; after the strain is poured, the system is rapidly started; and the strain has broad application prospects in various wastewater denitrogenation treatment processes.

The invention relates to a strain capable of degrading feather keratin efficiently and a screening method thereof. According to result of the analysis and identification of the sequence of 16SrDNA and the result of the identification of a Biolog system, F4 is determined to be Bacillus licheniformis and is named Bacillus licheniformis F4. The Bacillus licheniformis F4 was collected in China Center for Type Culture Collection with a collection number of CGMCC No.4229 on October 22th, 2010. The screening method comprises: primarily screening, separating, enriching, and secondarily screening to obtain the strain capable of degrading feather keratin efficiently. In the invention, the strain capable of degrading feather keratin efficiently is obtained by two steps of screenings under strict conditions, wherein primary screening conditions are a carbon source and a nitrogen source, and secondary screening conditions are complete degradation of feathers and measurement of enzymatic activity of keratin. The strain is inoculated into a fermentation culture medium containing complete feathers to perform fermentation culture for 60 hours, all barbules of the feathers are removed, the left feather stems and a major part of removed pinnules are degraded, and the feather removing and feather keratin degrading effects of the strain are obvious.

The invention discloses a bacterial strain b capable of resisting the growth of heavy metal Cd and reducing sulfate, and a bacterial strain capable of resisting the growth of the heavy metal Cd and solubilizing phosphate. The classification and name of the bacterial strain b and the bacterial strain are respectively enterbacter ludwigii SRB-2-5U-2 and pseudochrobactrum saccharolyticum LB-4-4-1c, which are already preserved in the Common Microbe Center of the China Committee for Culture Collection of Microorganisms. The bacterial strains disclosed by the invention can be used for simultaneously remediating the surface soil, deep soil and rhizosphere soil of a plant, reducing the pollution of Cd to surface water and underground water, and also improving the activity and fertility of the soil.

The invention discloses bacillus amyloliquefaciens JK6 and biological fertilizer and application. The serial number of strain preservation is CGMCC No. 10658 and is stored in the China general microbiological culture collection center on March 23, 2015. The bacillus amyloliquefaciens JK6 is mainly used for biological prevention and control of pseudomonas solanacearum and is provided with an iron-production carrier, a biological membrane, protease, catalase, cellulose and IAA characteristics, multiple fungal pathogens of banana wilt pathogen, litchi anthrax germs, litchi frost phytophthora pathogens, rice blast fungus, cucumber wilt pathogen and the like, and growth of lettuce plants is obviously promoted; the K6 strain can be successfully planted, and disease preventing functional genes of yndj, srfAB, fend and ituC are expressed in soil; the strain is high in reproduction speed, simple in production technology, high in adverse resistance, easy to store and favorable for industrialized production, the biological fertilizer is prepared with the strain, soil-born diseases can be prevented and controlled, growth of the plants can be promoted, and application prospect is broad.

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

The invention discloses a microorganism compound microbial inoculum, the strains of which comprise bacillus, soil actinomycetes, microzyme, mildew and photosynthetic bacteria. By using the microbial inoculum for straw fermentation, a result indicates that the microbial inoculum can make straws quickly decay and transfer to organic fertilizer required for the growth of plants, thereby the content of soil humus is increased, and the content of microorganism and trace elements in soil is improved. The physicochemical property of soil is improved, and the capability of water and fertility preserving of soil is increased. The soil permeability can also be improved so as to inhibit the happening of crop pests, and the output and quality of products can be improved. The microbial inoculum has the advantages of convenient use, low cost and obvious effect.

The invention discloses bacillus amyloliquefaciens and the microbial inoculum and application thereof. Bacillus amyloliquefaciens is a Latin name, the strain number is HC200, and the accession number in the China General Microbiological Culture Collection Center is CGMCC No.10371. The bacillus amyloliquefaciens has a selective antagonism effect on biocontrol bacteria and pathogenic bacteria and has a strong inhibiting effect on fusarium oxysporum, fusarium solani, phytophthora nicotianae and ralstonia solanacearum, and has a good synergetic antagonism effect on pathogenic bacteria by being matched with trichoderma viride H06.

The invention relates to Bacillus amyloliquefaciens and application thereof on aquiculture. The bacterial strain is separated from the substrate sludge of a polluted brook, is identified as the Bacillus amyloliquefaciens and is collected in Microscobial Culture Collection of China. The bacterial strain can normally grow within the temperature of 20 to 45 DEG C and within the pH value of 5.0 to 10.0; the bacterial strain can reach the fermentation terminal point when being inoculated on a broth culture medium the initial pH value of which is 7.0 according to inoculum concentration of 1:20 and cultured for 18 hours at the speed of 150r.min-1 at the temperature of 39 DEG C. The invention is characterized in that the bacterial strain can enable the content of nitrate nitrogen, nitrite nitrogen, COD and the like in a culture water body to be obviously lowered, has no harm to people and livestock and can be produced on a large scale for improving the aquiculture environment. The Bacillus amyloliquefaciens has obvious degradation function on main pollutants in a simulate culture water body and can be made into a micro-ecology preparation for purifying the culture water body.

The invention discloses bacillus amyloliquefaciens (Ba 168) and a preparation method and an application of the bacillus amyloliquefaciens, and aims at providing a biological control method of phytopathogens. The bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.6462 on August 21, 2012. The bacterial strain is separated and obtained from soil in the Qinling Mountain virgin forest, and subjected to bacteria activation, shake-flask fermentation, seed liquid fermentation and large tank fermentation to obtain fermentation liquid; and the fermentation liquid is subjected to filtration and concentration, ultrafiltration membrane separation, and spray drying and packaging to be prepared into bactericide. The bacillus amyloliquefaciens has the advantages that the bacillus amyloliquefaciens can well prevent and cure bighead atractylodes rhizome root rot, American ginseng root rot, pseudo-ginseng root rot and other root rot caused by fusarium oxysporum.

The invention discloses a compound bacterium for preventing and treating root-knot nematode and a preparation method thereof. The compound bacterium for preventing and treating root-knot nematode is prepared by the following steps: carrying out high-density cultivation on raw material strains of bacillus thuringiensis, pseudomonas fluorescens, bacillus subtilis, streptomycete, aspergillus niger, paecilomyces lilacinus and fusarium oxysporum, inoculating activated and cultivated strains in a fermentation tank and implementing fermentation and extended cultivation; dehydrating and drying various single strains obtained from extended cultivation to obtain resting microorganism dry powder; and mixing 10-20 parts of bacillus thuringiensis, 5-10 parts of pseudomonas fluorescens, 10-20 parts of bacillus subtilis, 10-20 parts of streptomycete, 10-15 parts of aspergillus niger, 10-25 parts of paecilomyces lilacinus and 8-15 parts of fusarium oxysporum to obtain the compound bacterium for preventing and treating root-knot nematode. The compound bacterium disclosed by the invention is used for preventing and treating root-knot nematode; and compared with an existing product, the compound bacterium is environment-friendly, harmless and non-toxic to a human body and good in control efficiency.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion / g to 1 billion / g and an organic matter content of 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.