182 results about "Biomineralization" patented technology

The invention relates to the technical field of biological induction of calcium carbonate deposition, in particular to self-repairing concrete for biological induction of calcium carbonate deposition and a preparation method thereof. The cement comprises the following components in parts by weight: 320-380 parts of Portland cement and the like. The biomineralization composite inducer contains a genetically engineered bacterium solution, a urea-calcium chloride solution and a nano strontium carbonate seed crystal; the nano silicon dioxide-graphene composite powder is prepared by chemical vapor deposition; the wall material of the double-response microcapsule contains a temperature-sensitive polymer, and the core material contains a plurality of components; nanocellulose whiskers are also added; self-repairing is achieved through cooperation of multiple components, and all the components are specially treated or prepared. The self-repairing concrete is high in self-repairing efficiency, and wide cracks can be repaired; the mechanical property is excellent, and the compression strength and the breaking strength are remarkably improved; working performance is good, and construction is convenient; durability is good, and erosion and permeability are resisted; environmental protection and economy are considered, and the application prospect is wide.

The invention discloses an in-situ biomineralizable mineral-PHA water-based seed coating based on microphase separation and soil water potential response and a preparation method and application thereof, and belongs to the technical field of green coatings, the in-situ biomineralizable mineral-PHA water-based seed coating comprises a structural mineral skeleton, a PHA and resin composite matrix and a pore-forming disintegrating agent. According to the invention, a resin enrichment layer and island microphase structure is innovatively constructed, and the contradiction between poor wear resistance and difficult disintegration of a bio-based material is effectively solved. Compared with the prior art, the wear-resistant and dust-reducing coating has the advantages that the wear-resistant and dust-reducing performance far better than that of the traditional PHA coating is realized by utilizing an interface enhancement effect under the condition of equal or even lower polymer dosage; meanwhile, the coating is endowed with a sensitive soil water potential response'switch ', so that dry-state tough seed protection and wet-state rapid disintegration are ensured, and germination is not hindered. Organic components of the composition can be biodegraded in aerobic soil, inorganic components are natural minerals, the environmental compatibility is good, the composition has the advantages of high performance and low cost, and an excellent seed treatment scheme is provided for green agriculture.

The invention relates to the technical field of embankment reinforcing and widening, and provides an embankment widening construction method based on biomineralization and reinforcement cooperation. The microcosmic cementation effect of calcium carbonate precipitation in the inclusion is induced by the enzyme and is combined with the macroscopic constraint of the geogrid, so that a friction-cementation composite interface is formed, and the shear resistance and impermeability of the new and old roadbed combined surface are greatly improved. Meanwhile, the synergistic enhancement transition area serves as a rigidity coordination layer, loads can be effectively homogenized, differential deformation can be effectively absorbed, and therefore differential settlement is restrained, and reflection cracks are prevented. According to the method, the microcosmic cementation strengthening capacity of enzyme-induced calcium carbonate precipitation and the macroscopic reinforcement restraining capacity of the geotechnical reinforcement material are subjected to space coupling and function cooperation, the weak area of the widened embankment is fundamentally improved, and the integrity and long-term stability of the widened embankment are remarkably improved.

The invention relates to a method for repairing light rare earth and heavy metal compound polluted soil, which is characterized in that the compound polluted soil is repaired by adopting bacillus sp., the bacillus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22085, and the preservation date is March 29, 2021. The repairing process comprises the following steps: mixing the bacillus subtilis bacterial liquid with the cementing liquid to obtain a mixed liquid; mixing the air-dried polluted soil with the mixed solution, and filling the mixture into a mold for reaction; the mold is communicated with a grouting pipe to inject the mixed liquid, and multi-round pumping is carried out; and after pumping is completed, the polluted soil is maintained and naturally air-dried. According to the method, dominant strains are adopted, biological adsorption and biological mineralization are combined, and effective control over the light rare earth and heavy metal compound contaminated soil is achieved under the action of dual bioremediation.

The invention relates to the technical field of material performance detection, and discloses a non-destructive evaluation method and system for the strength of microorganism mineralized river sand, and the method comprises the following steps: applying a sweep frequency sound wave excitation signal to a microorganism mineralized river sand standard sample model; performing penetration simulation operation on the microorganism mineralized river sand standard sample model according to the sweep frequency sound wave excitation signal; extracting mineralization specific characteristic parameters corresponding to the sample model through the penetration response signal; according to the mineralization specificity characteristic parameters, constructing a biological cementation index of microorganism mineralization, and according to the biological cementation index, analyzing the compressive strength of the microorganism mineralization river sand standard sample model; constructing a destructive calibration curve of the microorganism mineralized river sand standard sample model; the target compressive strength of the to-be-tested sample model is analyzed through the destructive calibration curve, and the strength non-destructiveness of the microorganism river sand corresponding to the to-be-tested sample model is analyzed based on the target compressive strength. According to the method, the accuracy of non-destructive evaluation of the strength of the microorganism mineralized river sand can be improved.

The invention discloses an ecological slope protection material, system and method based on struvite biomineralization technology.The ecological slope protection material comprises a slope stabilization material and a vegetation matrix, the slope stabilization material is composed of an enzyme-induced phosphate precipitation reaction solution and an enzyme-induced carbonate precipitation reaction solution, and through the synergistic effect of the two biomineralization technologies, the slope stabilization material can be used for slope protection. A biological mineralization reaction occurs in the side slope to generate struvite, magnesium carbonate and other minerals, so that side slope tailing sand reinforcement and heavy metal stabilization are realized, and slow-release nutrition is provided for plant growth. The invention further constructs a multi-layer slope protection system comprising a slope lattice beam, an in-situ remediation area, a slope drainage layer and a slope vegetation layer, and the multi-layer slope protection system is achieved through a construction method combined with a special grouting-sprinkling irrigation device. And finally, integrated safety reinforcement and ecological regreening of the side slope of the heavy metal tailings pond are achieved.

The invention provides a method for solidifying soft soil through the biological mineralization effect of guar gum and a urease-producing strain, the urease-producing strain is bacillus pasteurii DC419, and the preservation number of the urease-producing strain is CCTCC (China Center for Type Culture Collection) NO: M 20222038. The method for solidifying the soft soil comprises the following steps: mixing urease-producing strain fermentation liquor and cementing liquid to obtain mixed liquor; soft soil and guar gum are evenly mixed, the mixed solution is added, the mixture is evenly mixed and then stands still, and solidification of the soft soil is achieved. The soft soil cured by the method disclosed by the invention has relatively high compressive strength and relatively good waterproof capability.

The invention discloses a concrete structure crack control repairing method under unfavorable geological conditions, and relates to the technical field of concrete structure crack repairing, and the method comprises the following steps: S1, crack pretreatment: adopting a laser-ultrasonic wave composite cleaning technology to remove impurities of cracks, and combining a three-dimensional CT scanning technology to mark crack three-dimensional distribution and risk grade; s2, intelligent grouting repair; s3, surface nano coating; s4, releasing and reinforcing stress; s5, enhancing biomineralization; s6, installing an intelligent monitoring system; s7, layered grouting treatment; and S8, regularly maintaining and dynamically repairing. Through the synergistic effect of the step S1, the step S2 and the step S3, accurate control over the whole process from crack cleaning, filling to surface protection is achieved, compared with traditional high-pressure water cleaning, the surface roughness is improved, and meanwhile an accurate foundation is laid for follow-up repairing; through the steps S4, S6 and S8, a closed-loop maintenance system with active stress regulation and control, real-time monitoring and autonomous healing is constructed, a microcapsule self-repairing agent is released to a high-risk area, and the self-healing efficiency is improved.

The invention belongs to the technical field of building materials, and particularly relates to a microorganism mineralization modified recycled aggregate as well as a preparation method and application thereof. The preparation method of the microorganism mineralization modified recycled aggregate provided by the invention comprises the following steps: mixing a bacterium solution of microorganisms with nitrification and denitrification capabilities with a bacterium solution of urealytic bacteria for the first time to obtain a mixed bacterium solution; the isoconcentration bacterial liquid volume ratio of the microorganisms with nitrification and denitrification capabilities to the urealysis bacteria in the mixed bacterial liquid is (2: 1)-(1: 4); mixing the mixed bacterial liquid, the recycled aggregate and a mineralizing liquid for a second time, and then performing biological mineralization to obtain the microorganism mineralized modified recycled aggregate; the mineralization liquid comprises urea and calcium formate. The recycled aggregate is subjected to microbial mineralization under the combined action of microorganisms with nitrification and denitrification capacities and urealysis bacteria in a specific proportion, a calcium carbonate protection layer can be generated, the strength of the recycled aggregate is improved, the generated ammonia gas is less, and large-scale application is facilitated.

Disclosed herein is a biomineral supplemental blend for the replacement of hydraulic cement to create sustainable controlled low strength material (CLSM) with composition comprising a biomineralized algae, a silica source, pozzolan, biochar and / or bio-fly ash; and an aggregate; where the aggregate comprises a secondary binder, sand and / or rock, hydraulic cement, and filler material(s). Also disclosed herein is a method of manufacturing a transportable dry biomineral supplemental blend CLSM dry paste composition comprising blending together a microorganism package, a biomineralized algae, biochar and / or bio-fly ash, and pozzolan; mixing the biomineral supplemental blend, with hydraulic cement, cementitious materials, and liquid media to form a sustainable CLSM paste; and mixing the sustainable CLSM paste with an aggregate to form a sustainable CLSM mix design.

The invention provides a microbial dust suppressant based on infiltration-consolidation, a preparation method and a dust suppression method. The dust suppressant specifically comprises a cementing material, mineralizing bacteria, a nutrient solution and a cementing solution. The preparation method comprises the following steps: inoculating mineralized bacteria into a nutrient solution to obtain a mineralized bacteria solution, and then adding gelling materials with different concentrations to obtain a gelling material-mineralized bacteria solution; then, a calcium source-urea mixed cementing liquid is added, a gelling-microbial dust suppression material is obtained, and performance improvement of the microbial dust suppressant based on infiltration-consolidation is achieved through the coupling effect of the process of inducing calcium carbonate precipitation through microorganisms and the hydration reaction of the gelling material. According to the gelling-microorganism dust suppression material disclosed by the invention, not only can the infiltration process be remarkably enhanced by utilizing the gelling effect of the gelling material to play a role in regulating and controlling the infiltration performance, but also the synergistic dust suppression of the gelling material and the mineralizing bacteria can be realized by combining the biomineralization effect of the mineralizing bacteria, so that the dust consolidation effect is effectively improved.

The invention belongs to the technical field of soil amendments, and particularly relates to a saline-alkali soil biological-mineral composite amendment prepared from industrial waste and a preparation method thereof. Alkali fusion activated steel slag provides a quick-acting active calcium source, hydrothermal modification neutralized red mud forms hydrated aluminosilicate gel solid sodium, modified biochar enhances cation exchange capacity, a composite functional bacterial agent drives MICP biomineralization reaction, a quick-acting-slow-release-storage three-stage gradient improvement system is established, and the quick-acting-slow-release-storage three-stage gradient improvement effect is achieved. According to the present invention, the effects of 0-50 cm soil profile full-layer improvement and 12-24 month continuous improvement are achieved, 90 d after the modifier is applied, the soil pH value of the severe saline-alkali soil is reduced to 7.8 from 10.1, the conductivity is reduced to 3.2 dS / m from 11.5 dS / m, the sodium adsorption ratio is reduced to 11 from 42, the crop yield increase amplitude reaches 40-60%, 15-25 kg of the modifier is fixed per ton, and the industrial waste resource utilization rate gt is achieved; 90%.

The invention discloses a vulcanized porous activated carbon composite material as well as a preparation method and application thereof, and belongs to the technical field of inorganic functional materials. The preparation method comprises the following steps: synthesizing copper sulfide / chitosan quaternary ammonium salt composite particles by adopting a biomineralization strategy, depositing the copper sulfide / chitosan quaternary ammonium salt composite particles on a biomass porous activated carbon material to obtain a sulfurized porous activated carbon composite material, and uniformly loading the copper sulfide / chitosan quaternary ammonium salt composite particles by taking the biomass porous activated carbon material as a carrier to obtain the composite material. The sulfurized porous activated carbon composite material shows excellent photothermal conversion performance, high temperature is generated after light treatment, and the structure of the biomass porous activated carbon material and the structure of the chitosan quaternary ammonium salt are combined, so that the sulfurized porous activated carbon composite material not only shows strong adsorption capacity on organic pollutants in sewage, but also has strong adsorption capacity on the organic pollutants in the sewage. Meanwhile, bacteria attached to the surface of the fabric can be effectively inhibited and killed.

The present application belongs to the technical field of solid waste resource utilization, and relates to a kind of microbial mineralization solid waste based three-level interpenetrating heat storage body and its preparation method.The heat storage body includes microporous silica skeleton, and the solid waste activation component loaded in its interior;The solid waste activation component includes three-level functional material and red mud matrix;With the total mass of solid waste activation component being 100%, red mud matrix accounts for 30%-50%, and three-level functional material accounts for 50%-70%;With the total mass of three-level functional material being 100%, three-level functional material includes: 50%-60% titanium dioxide quantum dots, 20%-30% gallium oxide exciton, 10%-20% activated phosphogypsum sulfate powder;Three-level functional material forms three-level interpenetrating network structure in microporous silica skeleton.In the prior art, there is a problem in the solid waste treatment method that cannot realize the application of solid waste reenergy storage field through quantum dot and exciton coupling mechanism in solid waste.

The invention discloses an all-dimensional spraying vibration grouting sample preparation device based on MICP reinforced sandy soil. The device is composed of an all-dimensional spraying system, a grouting auxiliary system and a vibration system. The omni-directional spraying system comprises a conveying pump, a liquid distribution box, a regulation and control nozzle and a liquid storage bin; the grouting auxiliary system comprises an intelligent program control board, a circuit integrated carrier cover, a circular box body, a square base, a net-shaped thin-wall sample loading barrel, an annular drainage table and a waste liquid flow guide pipe; the vibration system comprises a miniature variable-frequency electromagnetic vibration motor and a buffer assembly table. In the process of biologically mineralizing sandy soil, the sandy soil is filled into the net-shaped thin-wall sample loading barrel, the circuit integrated carrier cover is closed, the omnibearing spraying system and the vibration system are accurately controlled through the intelligent program control panel, and the cementing liquid and the bacterial liquid are uniformly permeated among sand particles. The problem of uneven deposition of calcium carbonate in the MICP microbial mineralization process can be effectively solved, so that the mineralization efficiency and quality are improved.

The application discloses a kind of solid sealing chromium composite cementitious material and preparation method thereof, solid sealing chromium composite cementitious material includes the following mass percentage of substance: 65wt.%~75wt.% of clinker with heavy metal content, 20wt.%~34wt.% of poly-element auxiliary cementitious material, the rest is gypsum;Total chromium content in clinker with heavy metal content is greater than 150mg / kg;Poly-element auxiliary cementitious material includes the following mass percentage of substance: 30wt.%~50wt.% of microbial mineralization steel slag powder, 20wt.%~40wt.% of metakaolin, the rest is fly ash;Microbial mineralization steel slag powder is prepared by carbonic anhydrase producing bacteria and steel slag powder carbon fixation grinding.The application is based on the synergistic effect between clinker with heavy metal and the overall chemical composition of poly-element composite auxiliary cementitious material, and the pH value of solidified body is reduced by secondary hydration.

This utility model discloses a slurry proportioning adjustment device for biomineralization, including a mixing tank and a support frame. The support frame is located at the center of each of the four sides of the top of the mixing tank. A support cover is provided at the bottom of the mixing tank via legs. A stirring shaft is located at the center of the mixing tank, with stirring rods evenly distributed on the stirring shaft. Rotating rods are located at the center of both sides of the support cover, and stirring rods are evenly distributed on the rotating rods inside the mixing tank. An annular tube is located at the top of the mixing tank, and an inlet pipe connected to an external water storage tank is located on one side of the top of the annular tube. An annular toothed block is located on the top of the mixing tank inside the annular tube, and an L-shaped brush facing the annular tube is located at the center of one side of the bottom of the annular toothed block. This utility model improves the uniformity of the slurry by achieving more comprehensive mixing, and the annular tube can be cleaned after mixing to prevent nozzle clogging.

The invention discloses a medical polyaryletherketone implant and a surface modification method thereof, and the method comprises the following steps: immersing a polyaryletherketone material matrix into a saturated sodium carbonate solution used as a foaming agent, taking out the polyaryletherketone material matrix, and carrying out concentrated sulfuric acid ultrasonic treatment at room temperature; alternately treating with a saturated sodium carbonate solution and concentrated sulfuric acid until a microporous network is formed on the surface of the polyaryletherketone material matrix; carrying out surface phosphorylation on the polyaryletherketone material matrix with the microporous network formed on the surface by using a diazo reaction to obtain a surface phosphorylated matrix; treating the surface phosphorylated matrix by adopting a hydrothermal method and a chemical coprecipitation method, and forming a biomineralization layer doped with antibacterial metal ions on the surface of the matrix; and freeze-drying a hydrogel coating doped with antibacterial fatty acid on the surface of the matrix to obtain the medical polyaryletherketone implant.

The invention provides a method for synchronously removing suspended matters and hardness ions in water based on biomineralization, which comprises the following steps: high-hardness wastewater and a pre-mineralized urease-producing bacterium liquid are mixed and cultured, the pre-mineralization is to form a calcium carbonate shell on the surface of the urease-producing bacterium, and the urease-producing bacterium can hydrolyze urea to generate carbonate ions and ammonium ions. A mineralized shell is formed through the pre-mineralization process to protect the strain, reduce the influence of pollutants in the environment on the urease activity of the strain, increase the self weight of the strain and promote co-sedimentation of thalli and suspended solids in wastewater, hardness ions in the wastewater are converted into carbonate solid minerals through biological mineralization, removal of the hardness ions is achieved, and the removal rate of the wastewater is increased. Meanwhile, heavy metals and organic pollutants in the wastewater can be removed in the biomineralization process of the urease-producing bacteria. According to the method, synchronous removal of hardness ions and suspended solids in the wastewater is realized based on a biomineralization treatment process, and the method is environment-friendly, low in cost and easy for large-scale application.

The invention discloses a desertified soil restoration method based on microorganism mineralization induction and moss-algae crust, which comprises the following steps: crushing soybeans, and dissolving the crushed soybeans in water to obtain a soybean urease crude extract; adding desert green algae and drought-enduring moss into the cementing liquid prepared from the travertine for later use; the algae crust cementing liquid is obtained; the method comprises the following steps: preparing a soybean urease crude extracting solution, uniformly mixing the soybean urease crude extracting solution with desertification soil needing to be recovered, adding a moss-algae crust cementing solution into the surface layer of the mixed desertification soil, and curing for a period of time to realize the solidification of the desertification soil and the moss-algae crust on the surface layer. On the basis of microorganism mineralization induced sand stabilization, the bryophyllum crust is further applied to the soil surface to enhance the compressive strength of the soil surface, so that the strength of the desertification soil can be effectively recovered, and meanwhile, the bryophyllum crust also enables the desertification soil to be covered by moss and algae again, and the nutrients and environment of the soil are recovered.

The application discloses a method for repairing light rare earth and heavy metal composite contaminated soil, and adopts bacillus megaterium (Bacillus megaterium) to repair the composite contaminated soil. Oceanobacillus sp. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No.22085, and is preserved on March 29, 2021. The repairing process is as follows: mixing bacillus megaterium liquid and cementing liquid to obtain a mixed liquid; mixing the air-dried contaminated soil and the mixed liquid, and then loading the mixed liquid into a mold for reaction; connecting a grouting pipe to the mold to inject the mixed liquid, and performing multiple rounds of pumping; after the pumping is completed, the contaminated soil is maintained and air-dried naturally. The application adopts the advantage strain, combines biological adsorption and biological mineralization, and realizes the effective control of the light rare earth and heavy metal composite contaminated soil under the action of double biological repair.

The application discloses a kind of composite materials for 3D printing wild animal skeleton, which is composed of composite nanoparticles of calcium phosphate and hydroxyapatite, and is prepared by a combined method of biomimetic in-situ synthesis-solvent thermal phase inversion-spray drying method.The composite material has high specific surface area and excellent fluidity, and can be used for 3D printing wild animal skeleton, with broad market application prospect.

This application relates to the field of marine engineering technology and discloses a marine cofferdam anti-scour structure. The structure includes permanent support piles formed by modifying temporary cofferdam steel pipe piles; a riprap protection body constructed around the support piles; and a capping concrete layer poured on top of the riprap protection body. The riprap protection body is a differentiated structure. In scour hotspot areas calculated based on a hydrodynamic model, its upper riprap layer consists of a mixture of large-diameter boulders and a bio-induced matrix. The bio-induced matrix contains a slow-release marine microbial nutrient binder, which can induce biomineralization in the marine environment to cement loose boulders into a biomimetic reef body with self-healing capabilities. The construction method of this invention correspondingly includes hydrological optimization design and the formation of permanent support piles. This invention achieves precise protection against scour risks through the synergistic optimization of structure and construction method, improving the long-term stability and durability of the cofferdam foundation.

The invention discloses a slope biomineralization protection method and system.The method comprises the steps that a bentonite-carbon nano tube composite carrier, a signal capsule and a reaction capsule are prepared, the bentonite-carbon nano tube composite carrier is obtained by treating ultrasonically-dispersed bentonite and multi-wall carbon nano tubes according to a preset mixing proportion, and the signal capsule and the reaction capsule are filled with the bentonite-carbon nano tube composite carrier; the specific surface area of the bentonite-carbon nanotube composite carrier is 800 to 1000 m / g; combining the bentonite-carbon nanotube composite carrier with the germination capsule to obtain the germination capsule containing the composite carrier; mixing the signal capsule, the reaction capsule and the germination capsule containing the composite carrier with graded fine sand and chopped basalt fiber to obtain a capsule-aggregate mixture; and the capsule-aggregate mixture is laid on the surface of the slope, planting soil is covered, vegetation is planted, and three-level time sequence repair is triggered through rainwater infiltration. The stability and the high efficiency of slope biomineralization protection are improved.

The test device comprises a supporting frame and a test cylinder installed at the upper end of the supporting frame, a permeable base plate is detachably connected to the position, close to an opening in the lower end of the test cylinder, of the inner wall of the test cylinder, and a multi-stage inverted filter layer is detachably connected to the upper end of the permeable base plate; a plurality of monitoring assemblies and thermocouples are arranged on the inner wall of the test cylinder, a lower end opening of the test cylinder is communicated with a liquid guide hopper in a matched mode, a negative pressure regulation and control assembly is arranged on one side of the supporting frame, a water spraying assembly is detachably connected to the upper end of the outer wall of the test cylinder, a liquid supply assembly is arranged on the other side of the supporting frame, and a micropore injection and infiltration assembly is arranged on one side of the water spraying assembly. A plurality of guide grooves are formed in the inner wall of the test cylinder, the outer wall of the test cylinder is wrapped with a temperature control assembly, and a control cabinet is installed on one side of the upper end of the supporting frame. According to the invention, the unsaturated infiltration process under complex natural conditions such as rainfall and underground water level change can be simulated, slurry can be uniformly distributed in a soil body, and the discreteness of a mineralization effect is reduced.

The application discloses a preparation method of a high-efficiency bio-mineralized recycled construction waste mixture under load action, adopts a hydrostatic pressure loading reactor, carries out gradient hydrostatic pressure domestication on a starting strain, and obtains a high-load-resistant strain; the high-load-resistant strain is cultured in a large scale to obtain a high-pressure-resistant bacterial liquid; soluble calcium salt, urease activator, crystal form regulator and water are mixed to prepare a composite inducer; the high-pressure-resistant bacterial liquid and the composite inducer are uniformly mixed according to a volume ratio to obtain a mineralization mixing liquid; recycled construction waste aggregates and cementing materials are weighed and put into a stirring device, the mineralization mixing liquid is added into the stirring device as mixing water to be stirred, and pressure forming and curing are carried out after uniform stirring to obtain the recycled construction waste mixture. Through directional screening of the pressure-resistant strain and preparation of a rapid induction system, the application solves the problems of inactivation of microorganisms and low mineralization efficiency under extrusion load, and the aggregates do not need to be pretreated and can be directly integrated into the existing engineering mixing process.

The invention discloses a side slope biomineralization protection system and method based on a double-response type signal capsule, and the method comprises the steps: preparing the double-response type signal capsule, a germination capsule and a reaction capsule respectively, a wall material of the double-response type signal capsule is obtained by mixing poly (N-isopropylacrylamide) and xanthan gum according to a preset proportion; mixing the double-response signal capsule, the germination capsule and the reaction capsule with graded fine sand and chopped basalt fiber to obtain a capsule-aggregate mixture; the capsule-aggregate mixture is laid on the surface of the slope, planting soil is covered, vegetation is planted, and when the preset temperature-water content condition is met, slope time sequence repairing is triggered. According to the scheme, the temperature and the moisture can be responded at the same time, and the complex climatic environment can be accurately adapted.

This invention relates to a semi-rigid vertical barrier reinforced by synergistic microbial mineralization and carbide slag, and its preparation method. The raw materials include calcium-based bentonite, in-situ soil, urease-inducing bacteria activator, and carbide slag. The dry weight of the calcium-based bentonite accounts for 15.0%–25.0% of the dry weight of the in-situ soil, the total dry weight of all components in the urease-inducing bacteria activator accounts for 2.0%–4.0% of the dry weight of the in-situ soil, and the dry weight of the carbide slag accounts for 3.0%–6.0% of the dry weight of the in-situ soil. This invention addresses the shortcomings of microbial mineralization technology in improving and reinforcing fine-grained soil, significantly improving the strength and impermeability of calcium-based bentonite-based barrier materials. Simultaneously, it utilizes ammonium ions, a byproduct of the microbial mineralization process, reducing the cost and carbon emissions of the barrier material.

The invention belongs to the technical field of biology, and particularly relates to an efficient extraction method of mineralized tissue RNA applicable across animal categories. The invention provides a solution combination suitable for mineralized tissue pretreatment, which not only is beneficial to promoting the release of cells from a mineralized matrix, but also can inhibit the activity of ribonuclease to a certain extent, and is beneficial to extracting high-quality RNA (Ribonucleic Acid). Furthermore, the invention also provides an efficient extraction method of mineralized tissue RNA suitable for cross-animal categories, the sample is pretreated by using a solution combination, and then RNA extraction, precipitation and other treatments are sequentially performed, so that the RNA extraction efficiency of the exoskeleton sample is remarkably improved, and the method has good universality and is suitable for large-scale popularization and application. The method is suitable for extracting RNA in cross-animal mineralized tissues, has important biological significance, and lays a methodological foundation for subsequent development of transcriptomics research of exoskeleton dynamic development, analysis of an environmental adaptation mechanism and construction of a biomineralization process regulation and control network.

This invention discloses a cerium-doped diatom-based silicon-carbon anode material, its preparation method, and its application, belonging to the technical field of lithium battery anode materials and their preparation. Using a diatom shell as a framework, a bio-doping method is employed. When the diatoms grow to the early logarithmic phase in a culture medium, cerium ammonium nitrate is added to the diatom culture medium for co-cultivation. The co-cultivated diatom shells are then calcined at high temperature to obtain the cerium-doped diatom-based silicon-carbon anode material. This invention employs a two-stage cultivation strategy, introducing cerium ammonium nitrate after the diatoms have reached the early logarithmic phase to avoid toxic inhibition during the growth-sensitive period. Simultaneously, it utilizes the efficient absorption and doping capabilities of diatoms during the active biomineralization phase, achieving a balance between high doping efficiency and structural integrity. This method utilizes the biomineralization capabilities of diatoms to achieve uniform elemental doping, resulting in a simple, low-cost, and environmentally friendly process; providing a new approach for the development of high-performance silicon-carbon anode materials.