211 results about "Biomineralization" patented technology

The invention relates to the technical field of biological induction of calcium carbonate deposition, in particular to self-repairing concrete for biological induction of calcium carbonate deposition and a preparation method thereof. The cement comprises the following components in parts by weight: 320-380 parts of Portland cement and the like. The biomineralization composite inducer contains a genetically engineered bacterium solution, a urea-calcium chloride solution and a nano strontium carbonate seed crystal; the nano silicon dioxide-graphene composite powder is prepared by chemical vapor deposition; the wall material of the double-response microcapsule contains a temperature-sensitive polymer, and the core material contains a plurality of components; nanocellulose whiskers are also added; self-repairing is achieved through cooperation of multiple components, and all the components are specially treated or prepared. The self-repairing concrete is high in self-repairing efficiency, and wide cracks can be repaired; the mechanical property is excellent, and the compression strength and the breaking strength are remarkably improved; working performance is good, and construction is convenient; durability is good, and erosion and permeability are resisted; environmental protection and economy are considered, and the application prospect is wide.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention discloses an in-situ biomineralizable mineral-PHA water-based seed coating based on microphase separation and soil water potential response and a preparation method and application thereof, and belongs to the technical field of green coatings, the in-situ biomineralizable mineral-PHA water-based seed coating comprises a structural mineral skeleton, a PHA and resin composite matrix and a pore-forming disintegrating agent. According to the invention, a resin enrichment layer and island microphase structure is innovatively constructed, and the contradiction between poor wear resistance and difficult disintegration of a bio-based material is effectively solved. Compared with the prior art, the wear-resistant and dust-reducing coating has the advantages that the wear-resistant and dust-reducing performance far better than that of the traditional PHA coating is realized by utilizing an interface enhancement effect under the condition of equal or even lower polymer dosage; meanwhile, the coating is endowed with a sensitive soil water potential response'switch ', so that dry-state tough seed protection and wet-state rapid disintegration are ensured, and germination is not hindered. Organic components of the composition can be biodegraded in aerobic soil, inorganic components are natural minerals, the environmental compatibility is good, the composition has the advantages of high performance and low cost, and an excellent seed treatment scheme is provided for green agriculture.

The invention discloses application of a mineralized carbon sequestration strain, the mineralized carbon sequestration strain is classified and named as Halomonas sulfidaeresis. ZN2 #, the mineralized carbon sequestration strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32033. The invention further discloses a preparation method of the mineralized carbon sequestration strain. The mineralization carbon sequestration strain can metabolize to generate urease and carbonic anhydrase at the same time, the two enzymes are subjected to a dual mineralization reaction in combination with a microorganism-induced calcium carbonate precipitation technology, the crystal form of a mineralization product is improved, the mineralization strength is improved, meanwhile, carbonic anhydrase can absorb carbon dioxide in the environment, high-strength soil reinforcement is achieved, meanwhile, carbon dioxide emission is reduced, and the soil quality is improved. Therefore, the dual effects of biomineralization remediation and improvement and carbon sequestration and emission reduction of the soil are achieved, and sustainable development of a soil bioremediation and improvement technology is achieved.

The invention relates to the technical field of embankment reinforcing and widening, and provides an embankment widening construction method based on biomineralization and reinforcement cooperation. The microcosmic cementation effect of calcium carbonate precipitation in the inclusion is induced by the enzyme and is combined with the macroscopic constraint of the geogrid, so that a friction-cementation composite interface is formed, and the shear resistance and impermeability of the new and old roadbed combined surface are greatly improved. Meanwhile, the synergistic enhancement transition area serves as a rigidity coordination layer, loads can be effectively homogenized, differential deformation can be effectively absorbed, and therefore differential settlement is restrained, and reflection cracks are prevented. According to the method, the microcosmic cementation strengthening capacity of enzyme-induced calcium carbonate precipitation and the macroscopic reinforcement restraining capacity of the geotechnical reinforcement material are subjected to space coupling and function cooperation, the weak area of the widened embankment is fundamentally improved, and the integrity and long-term stability of the widened embankment are remarkably improved.

The invention discloses a microorganism mineralization induced flood prevention preparation stone and a preparation method thereof. The microorganism mineralization induced flood prevention preparation stone is prepared from the following raw materials: a microorganism mineralization repairing agent, aluminum oxide, slag powder, silt, microorganism cementing liquid and steel fibers. The preparation method comprises the following steps: preparing the microbial mineralization repairing agent and the microbial cementing liquid, mixing the microbial mineralization repairing agent with alumina, adding the slag powder, stirring to obtain a composite gel material, mixing the composite gel material, the silt and the slag powder according to a preset ratio, adding the microbial cementing liquid to obtain mixed slurry, slowly adding the steel fibers into the mixed slurry, and stirring to obtain the composite gel material. And curing and demolding to obtain a sample, and curing to prepare the flood prevention preparation stone. The silt is used as a main raw material, microorganisms are added to accelerate mineralization and accelerate hydration of carbon dioxide, deconstruction and reunion of an active material are achieved, the pH value of a microorganism growth environment is reduced through aluminum oxide, microorganism adsorption sites are provided, and the corrosion resistance and strength of the stone are improved through the synergistic effect of aluminum oxide and steel fibers.

The invention relates to a method for repairing light rare earth and heavy metal compound polluted soil, which is characterized in that the compound polluted soil is repaired by adopting bacillus sp., the bacillus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22085, and the preservation date is March 29, 2021. The repairing process comprises the following steps: mixing the bacillus subtilis bacterial liquid with the cementing liquid to obtain a mixed liquid; mixing the air-dried polluted soil with the mixed solution, and filling the mixture into a mold for reaction; the mold is communicated with a grouting pipe to inject the mixed liquid, and multi-round pumping is carried out; and after pumping is completed, the polluted soil is maintained and naturally air-dried. According to the method, dominant strains are adopted, biological adsorption and biological mineralization are combined, and effective control over the light rare earth and heavy metal compound contaminated soil is achieved under the action of dual bioremediation.

The invention relates to the technical field of material performance detection, and discloses a non-destructive evaluation method and system for the strength of microorganism mineralized river sand, and the method comprises the following steps: applying a sweep frequency sound wave excitation signal to a microorganism mineralized river sand standard sample model; performing penetration simulation operation on the microorganism mineralized river sand standard sample model according to the sweep frequency sound wave excitation signal; extracting mineralization specific characteristic parameters corresponding to the sample model through the penetration response signal; according to the mineralization specificity characteristic parameters, constructing a biological cementation index of microorganism mineralization, and according to the biological cementation index, analyzing the compressive strength of the microorganism mineralization river sand standard sample model; constructing a destructive calibration curve of the microorganism mineralized river sand standard sample model; the target compressive strength of the to-be-tested sample model is analyzed through the destructive calibration curve, and the strength non-destructiveness of the microorganism river sand corresponding to the to-be-tested sample model is analyzed based on the target compressive strength. According to the method, the accuracy of non-destructive evaluation of the strength of the microorganism mineralized river sand can be improved.

The invention discloses an ecological slope protection material, system and method based on struvite biomineralization technology.The ecological slope protection material comprises a slope stabilization material and a vegetation matrix, the slope stabilization material is composed of an enzyme-induced phosphate precipitation reaction solution and an enzyme-induced carbonate precipitation reaction solution, and through the synergistic effect of the two biomineralization technologies, the slope stabilization material can be used for slope protection. A biological mineralization reaction occurs in the side slope to generate struvite, magnesium carbonate and other minerals, so that side slope tailing sand reinforcement and heavy metal stabilization are realized, and slow-release nutrition is provided for plant growth. The invention further constructs a multi-layer slope protection system comprising a slope lattice beam, an in-situ remediation area, a slope drainage layer and a slope vegetation layer, and the multi-layer slope protection system is achieved through a construction method combined with a special grouting-sprinkling irrigation device. And finally, integrated safety reinforcement and ecological regreening of the side slope of the heavy metal tailings pond are achieved.

The invention provides a method for solidifying soft soil through the biological mineralization effect of guar gum and a urease-producing strain, the urease-producing strain is bacillus pasteurii DC419, and the preservation number of the urease-producing strain is CCTCC (China Center for Type Culture Collection) NO: M 20222038. The method for solidifying the soft soil comprises the following steps: mixing urease-producing strain fermentation liquor and cementing liquid to obtain mixed liquor; soft soil and guar gum are evenly mixed, the mixed solution is added, the mixture is evenly mixed and then stands still, and solidification of the soft soil is achieved. The soft soil cured by the method disclosed by the invention has relatively high compressive strength and relatively good waterproof capability.

The invention discloses an in-situ remediation method for uranium pollution and application of the in-situ remediation method. According to the in-situ remediation method for uranium pollution, pyrite is driven to be hydrolyzed, oxidized, subjected to atomic rearrangement and biomineralized through interaction of microorganisms and pyrite, uranium pollutants are fixedly sealed and stored in reconstructed product iron oxide lattices, uranium pollution areas are remedied, and the microorganisms comprise one or more of brucella, geobacter and clostridium. If the uranium polluted area contains microorganisms, natural humus is used as an electron donor to stimulate in-situ microorganisms. The natural humus solution is injected into the uranium polluted area in a circulating pumping and injecting mode, in-situ microorganisms are stimulated, the concentration of the natural humus solution is 0.05-5 g / L, and the dosage of the natural humus solution is 5-500 g / day. According to the method, in-situ minerals, microorganisms and geochemical resources are fully considered and reasonably utilized, the engineering cost is saved by means of the in-situ occurrence interaction relation of the minerals and the microorganisms, and the remediation efficiency is high.

The invention discloses a concrete structure crack control repairing method under unfavorable geological conditions, and relates to the technical field of concrete structure crack repairing, and the method comprises the following steps: S1, crack pretreatment: adopting a laser-ultrasonic wave composite cleaning technology to remove impurities of cracks, and combining a three-dimensional CT scanning technology to mark crack three-dimensional distribution and risk grade; s2, intelligent grouting repair; s3, surface nano coating; s4, releasing and reinforcing stress; s5, enhancing biomineralization; s6, installing an intelligent monitoring system; s7, layered grouting treatment; and S8, regularly maintaining and dynamically repairing. Through the synergistic effect of the step S1, the step S2 and the step S3, accurate control over the whole process from crack cleaning, filling to surface protection is achieved, compared with traditional high-pressure water cleaning, the surface roughness is improved, and meanwhile an accurate foundation is laid for follow-up repairing; through the steps S4, S6 and S8, a closed-loop maintenance system with active stress regulation and control, real-time monitoring and autonomous healing is constructed, a microcapsule self-repairing agent is released to a high-risk area, and the self-healing efficiency is improved.

The invention belongs to the technical field of building materials, and particularly relates to a microorganism mineralization modified recycled aggregate as well as a preparation method and application thereof. The preparation method of the microorganism mineralization modified recycled aggregate provided by the invention comprises the following steps: mixing a bacterium solution of microorganisms with nitrification and denitrification capabilities with a bacterium solution of urealytic bacteria for the first time to obtain a mixed bacterium solution; the isoconcentration bacterial liquid volume ratio of the microorganisms with nitrification and denitrification capabilities to the urealysis bacteria in the mixed bacterial liquid is (2: 1)-(1: 4); mixing the mixed bacterial liquid, the recycled aggregate and a mineralizing liquid for a second time, and then performing biological mineralization to obtain the microorganism mineralized modified recycled aggregate; the mineralization liquid comprises urea and calcium formate. The recycled aggregate is subjected to microbial mineralization under the combined action of microorganisms with nitrification and denitrification capacities and urealysis bacteria in a specific proportion, a calcium carbonate protection layer can be generated, the strength of the recycled aggregate is improved, the generated ammonia gas is less, and large-scale application is facilitated.

The invention provides a microorganism curing fish-scale pit based on concrete waste and a preparation method of the microorganism curing fish-scale pit based on the concrete waste. The microorganism curing fish-scale pit comprises concrete broken materials, microorganism curing bacterium liquid, cementing liquid and natural coconut fiber, the strength and internal friction force of the whole material are increased, good attachment and biomineralization action points are provided for microorganisms, and a large number of microorganisms can generate calcium carbonate crystals to bond soil particles and short coconut fiber materials with the attachment points. The short coconut fiber is a reinforced body of the integrally cured material, has good toughness and tensile strength, and can enhance the mechanical strength and erosion resistance of the cured material, the fish-scale pit has good mechanical strength, erosion resistance, durability and ecological environmental protection property, the concrete waste and local soil are used as main materials, and the cost is reduced. And a whole is formed by utilizing a mineralization reaction principle of a microbial strain and natural coconut fibers, so that the requirements of energy conservation and low carbon are met.

The invention provides a marine microorganism mineralization coating and a preparation method thereof, and relates to the field of marine anti-corrosion coatings. The preparation method of the marine microorganism mineralization coating comprises the following steps: after carrying out sterile treatment on a target metal material, immersing the target metal material into a shewanella putrefaciens culture solution for first culture to obtain a metal material with a biological membrane; and adding L-calcium aspartate into the shewanella putrefaciens culture solution to obtain a biomineralization nucleation solution, and carrying out second culture to obtain the metal material with the marine microorganism mineralization coating. According to the method provided by the invention, an inorganic calcium source L-calcium aspartate is inoculated to regulate and control a carbonate layer formed by inducing shewanella putrefaciens. The form of the microorganism mineralization coating mediated and regulated by the L-calcium aspartate is converted into a flagstone shape from a general calcite crystal form, and the microorganism mineralization coating which is more compact, smoother and more stable is formed and has excellent corrosion resistance.

Disclosed herein is a biomineral supplemental blend for the replacement of hydraulic cement to create sustainable controlled low strength material (CLSM) with composition comprising a biomineralized algae, a silica source, pozzolan, biochar and / or bio-fly ash; and an aggregate; where the aggregate comprises a secondary binder, sand and / or rock, hydraulic cement, and filler material(s). Also disclosed herein is a method of manufacturing a transportable dry biomineral supplemental blend CLSM dry paste composition comprising blending together a microorganism package, a biomineralized algae, biochar and / or bio-fly ash, and pozzolan; mixing the biomineral supplemental blend, with hydraulic cement, cementitious materials, and liquid media to form a sustainable CLSM paste; and mixing the sustainable CLSM paste with an aggregate to form a sustainable CLSM mix design.

The invention provides a microbial dust suppressant based on infiltration-consolidation, a preparation method and a dust suppression method. The dust suppressant specifically comprises a cementing material, mineralizing bacteria, a nutrient solution and a cementing solution. The preparation method comprises the following steps: inoculating mineralized bacteria into a nutrient solution to obtain a mineralized bacteria solution, and then adding gelling materials with different concentrations to obtain a gelling material-mineralized bacteria solution; then, a calcium source-urea mixed cementing liquid is added, a gelling-microbial dust suppression material is obtained, and performance improvement of the microbial dust suppressant based on infiltration-consolidation is achieved through the coupling effect of the process of inducing calcium carbonate precipitation through microorganisms and the hydration reaction of the gelling material. According to the gelling-microorganism dust suppression material disclosed by the invention, not only can the infiltration process be remarkably enhanced by utilizing the gelling effect of the gelling material to play a role in regulating and controlling the infiltration performance, but also the synergistic dust suppression of the gelling material and the mineralizing bacteria can be realized by combining the biomineralization effect of the mineralizing bacteria, so that the dust consolidation effect is effectively improved.

The invention relates to the field of harmless treatment of dangerous solid wastes, in particular to a harmless treatment method for mineralizing cyanidation tailings through steel slag and microorganisms, which comprises the following steps: preparing a bacterial liquid, inoculating with sporosarcina pasteurii, and culturing to prepare a microbial bacterial liquid; steel slag-cyanidation tailing slurry is prepared, specifically, steel slag and cyanidation tailings are sequentially weighed according to the proportion and mixed to be uniform, then a microbial bacterium solution, a cementing solution and water are added, the mixture is stirred to be uniform, and microbial cementing filling slurry is obtained; and underground backfilling. The prepared underground backfill slurry removes cyanide in tailings, solidifies heavy metal, is good in cementing property, can be used as an underground backfill material for backfill, provides a new solution for resource utilization of cyanidation tailings, also promotes high additional utilization of solid waste steel slag, reduces environmental influence, provides potential value, and has a wide application prospect. And sustainable development is realized.

The invention belongs to the technical field of soil amendments, and particularly relates to a saline-alkali soil biological-mineral composite amendment prepared from industrial waste and a preparation method thereof. Alkali fusion activated steel slag provides a quick-acting active calcium source, hydrothermal modification neutralized red mud forms hydrated aluminosilicate gel solid sodium, modified biochar enhances cation exchange capacity, a composite functional bacterial agent drives MICP biomineralization reaction, a quick-acting-slow-release-storage three-stage gradient improvement system is established, and the quick-acting-slow-release-storage three-stage gradient improvement effect is achieved. According to the present invention, the effects of 0-50 cm soil profile full-layer improvement and 12-24 month continuous improvement are achieved, 90 d after the modifier is applied, the soil pH value of the severe saline-alkali soil is reduced to 7.8 from 10.1, the conductivity is reduced to 3.2 dS / m from 11.5 dS / m, the sodium adsorption ratio is reduced to 11 from 42, the crop yield increase amplitude reaches 40-60%, 15-25 kg of the modifier is fixed per ton, and the industrial waste resource utilization rate gt is achieved; 90%.

The invention discloses a vulcanized porous activated carbon composite material as well as a preparation method and application thereof, and belongs to the technical field of inorganic functional materials. The preparation method comprises the following steps: synthesizing copper sulfide / chitosan quaternary ammonium salt composite particles by adopting a biomineralization strategy, depositing the copper sulfide / chitosan quaternary ammonium salt composite particles on a biomass porous activated carbon material to obtain a sulfurized porous activated carbon composite material, and uniformly loading the copper sulfide / chitosan quaternary ammonium salt composite particles by taking the biomass porous activated carbon material as a carrier to obtain the composite material. The sulfurized porous activated carbon composite material shows excellent photothermal conversion performance, high temperature is generated after light treatment, and the structure of the biomass porous activated carbon material and the structure of the chitosan quaternary ammonium salt are combined, so that the sulfurized porous activated carbon composite material not only shows strong adsorption capacity on organic pollutants in sewage, but also has strong adsorption capacity on the organic pollutants in the sewage. Meanwhile, bacteria attached to the surface of the fabric can be effectively inhibited and killed.

The present application belongs to the technical field of solid waste resource utilization, and relates to a kind of microbial mineralization solid waste based three-level interpenetrating heat storage body and its preparation method.The heat storage body includes microporous silica skeleton, and the solid waste activation component loaded in its interior;The solid waste activation component includes three-level functional material and red mud matrix;With the total mass of solid waste activation component being 100%, red mud matrix accounts for 30%-50%, and three-level functional material accounts for 50%-70%;With the total mass of three-level functional material being 100%, three-level functional material includes: 50%-60% titanium dioxide quantum dots, 20%-30% gallium oxide exciton, 10%-20% activated phosphogypsum sulfate powder;Three-level functional material forms three-level interpenetrating network structure in microporous silica skeleton.In the prior art, there is a problem in the solid waste treatment method that cannot realize the application of solid waste reenergy storage field through quantum dot and exciton coupling mechanism in solid waste.

The invention discloses an all-dimensional spraying vibration grouting sample preparation device based on MICP reinforced sandy soil. The device is composed of an all-dimensional spraying system, a grouting auxiliary system and a vibration system. The omni-directional spraying system comprises a conveying pump, a liquid distribution box, a regulation and control nozzle and a liquid storage bin; the grouting auxiliary system comprises an intelligent program control board, a circuit integrated carrier cover, a circular box body, a square base, a net-shaped thin-wall sample loading barrel, an annular drainage table and a waste liquid flow guide pipe; the vibration system comprises a miniature variable-frequency electromagnetic vibration motor and a buffer assembly table. In the process of biologically mineralizing sandy soil, the sandy soil is filled into the net-shaped thin-wall sample loading barrel, the circuit integrated carrier cover is closed, the omnibearing spraying system and the vibration system are accurately controlled through the intelligent program control panel, and the cementing liquid and the bacterial liquid are uniformly permeated among sand particles. The problem of uneven deposition of calcium carbonate in the MICP microbial mineralization process can be effectively solved, so that the mineralization efficiency and quality are improved.

The application discloses a kind of solid sealing chromium composite cementitious material and preparation method thereof, solid sealing chromium composite cementitious material includes the following mass percentage of substance: 65wt.%~75wt.% of clinker with heavy metal content, 20wt.%~34wt.% of poly-element auxiliary cementitious material, the rest is gypsum;Total chromium content in clinker with heavy metal content is greater than 150mg / kg;Poly-element auxiliary cementitious material includes the following mass percentage of substance: 30wt.%~50wt.% of microbial mineralization steel slag powder, 20wt.%~40wt.% of metakaolin, the rest is fly ash;Microbial mineralization steel slag powder is prepared by carbonic anhydrase producing bacteria and steel slag powder carbon fixation grinding.The application is based on the synergistic effect between clinker with heavy metal and the overall chemical composition of poly-element composite auxiliary cementitious material, and the pH value of solidified body is reduced by secondary hydration.

This utility model discloses a slurry proportioning adjustment device for biomineralization, including a mixing tank and a support frame. The support frame is located at the center of each of the four sides of the top of the mixing tank. A support cover is provided at the bottom of the mixing tank via legs. A stirring shaft is located at the center of the mixing tank, with stirring rods evenly distributed on the stirring shaft. Rotating rods are located at the center of both sides of the support cover, and stirring rods are evenly distributed on the rotating rods inside the mixing tank. An annular tube is located at the top of the mixing tank, and an inlet pipe connected to an external water storage tank is located on one side of the top of the annular tube. An annular toothed block is located on the top of the mixing tank inside the annular tube, and an L-shaped brush facing the annular tube is located at the center of one side of the bottom of the annular toothed block. This utility model improves the uniformity of the slurry by achieving more comprehensive mixing, and the annular tube can be cleaned after mixing to prevent nozzle clogging.

The invention discloses a medical polyaryletherketone implant and a surface modification method thereof, and the method comprises the following steps: immersing a polyaryletherketone material matrix into a saturated sodium carbonate solution used as a foaming agent, taking out the polyaryletherketone material matrix, and carrying out concentrated sulfuric acid ultrasonic treatment at room temperature; alternately treating with a saturated sodium carbonate solution and concentrated sulfuric acid until a microporous network is formed on the surface of the polyaryletherketone material matrix; carrying out surface phosphorylation on the polyaryletherketone material matrix with the microporous network formed on the surface by using a diazo reaction to obtain a surface phosphorylated matrix; treating the surface phosphorylated matrix by adopting a hydrothermal method and a chemical coprecipitation method, and forming a biomineralization layer doped with antibacterial metal ions on the surface of the matrix; and freeze-drying a hydrogel coating doped with antibacterial fatty acid on the surface of the matrix to obtain the medical polyaryletherketone implant.

The invention discloses a method for immobilization and magnetic separation of arsenic in water by biomineralization, which comprises the following steps: S1, preparation of a liquid culture medium: dissolving a 60% (volume) sodium lactate solution, sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate and a yeast extract in deionized water in proportion, adjusting the pH value of the culture medium solution to 6.9-7.0, sub-packaging and performing high-pressure sterilization; s2, inoculating a bacteria solution into the sterilized culture medium for amplification culture; s3, preparing a standard arsenic solution; s4, preparing ferric citrate and ferrous chloride solutions; s5, performing MSR-1 biological control mineralization synthesis of magnetosome; 6, MSR-1 induces ferrous iron to precipitate and remove arsenic; and S7, magnetically separating a bacterium-iron-arsenic mixture. The method realizes fixation and separation of As in water, not only retains the advantages of low cost and environmental friendliness of biomineralization polluted water remediation, but also overcomes the defects of low remediation efficiency and potential pollution caused by incapability of recycling.

The invention provides a method for synchronously removing suspended matters and hardness ions in water based on biomineralization, which comprises the following steps: high-hardness wastewater and a pre-mineralized urease-producing bacterium liquid are mixed and cultured, the pre-mineralization is to form a calcium carbonate shell on the surface of the urease-producing bacterium, and the urease-producing bacterium can hydrolyze urea to generate carbonate ions and ammonium ions. A mineralized shell is formed through the pre-mineralization process to protect the strain, reduce the influence of pollutants in the environment on the urease activity of the strain, increase the self weight of the strain and promote co-sedimentation of thalli and suspended solids in wastewater, hardness ions in the wastewater are converted into carbonate solid minerals through biological mineralization, removal of the hardness ions is achieved, and the removal rate of the wastewater is increased. Meanwhile, heavy metals and organic pollutants in the wastewater can be removed in the biomineralization process of the urease-producing bacteria. According to the method, synchronous removal of hardness ions and suspended solids in the wastewater is realized based on a biomineralization treatment process, and the method is environment-friendly, low in cost and easy for large-scale application.

The invention discloses a desertified soil restoration method based on microorganism mineralization induction and moss-algae crust, which comprises the following steps: crushing soybeans, and dissolving the crushed soybeans in water to obtain a soybean urease crude extract; adding desert green algae and drought-enduring moss into the cementing liquid prepared from the travertine for later use; the algae crust cementing liquid is obtained; the method comprises the following steps: preparing a soybean urease crude extracting solution, uniformly mixing the soybean urease crude extracting solution with desertification soil needing to be recovered, adding a moss-algae crust cementing solution into the surface layer of the mixed desertification soil, and curing for a period of time to realize the solidification of the desertification soil and the moss-algae crust on the surface layer. On the basis of microorganism mineralization induced sand stabilization, the bryophyllum crust is further applied to the soil surface to enhance the compressive strength of the soil surface, so that the strength of the desertification soil can be effectively recovered, and meanwhile, the bryophyllum crust also enables the desertification soil to be covered by moss and algae again, and the nutrients and environment of the soil are recovered.

The application discloses a method for repairing light rare earth and heavy metal composite contaminated soil, and adopts bacillus megaterium (Bacillus megaterium) to repair the composite contaminated soil. Oceanobacillus sp. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No.22085, and is preserved on March 29, 2021. The repairing process is as follows: mixing bacillus megaterium liquid and cementing liquid to obtain a mixed liquid; mixing the air-dried contaminated soil and the mixed liquid, and then loading the mixed liquid into a mold for reaction; connecting a grouting pipe to the mold to inject the mixed liquid, and performing multiple rounds of pumping; after the pumping is completed, the contaminated soil is maintained and air-dried naturally. The application adopts the advantage strain, combines biological adsorption and biological mineralization, and realizes the effective control of the light rare earth and heavy metal composite contaminated soil under the action of double biological repair.