Calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and preparation method thereof

A technology of tissue engineering scaffold and bone-like apatite, which is applied in the field of biomedical materials, can solve the problems of comprehensive performance and bionic level gap, and achieve good bone formation ability, good mechanical properties, and the effect of promoting bone tissue repair and regeneration

Inactive Publication Date: 2013-10-09
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, research has been devoted to the development of collagen-based or biomaterials that use it as an additive phase for the repair of bone tissue, but the comprehensive performance and bionic degree of the obtained materials are still far from the application requirements.

Method used

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  • Calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and preparation method thereof
  • Calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and preparation method thereof
  • Calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A tertiary biomimetic scaffold for bone tissue engineering synthesized on the basis of porous hydroxyapatite ceramics. Its production specific steps are as follows:

[0050] 1) Customized porous calcium phosphate ceramic products. Product pore requirements: overall porosity 85%±5%, macropore diameter 400±50μm, interpenetrating micropore diameter 80±20μm, cut into thin discs of Φ15×2 mm;

[0051] a) The hydroxyapatite powder prepared by the wet reaction is screened with a standard type sieve to screen out the dry hydroxyapatite powder with a diameter of 80-160 μm, and then the porous ceramics of 20×20×50 mm are prepared by hydrogen peroxide foaming method Embryo body, low temperature <100 ℃ drying;

[0052] b) Put the ceramic green body into a muffle furnace for sintering, raise the temperature from room temperature to 1100 °C at a rate of 5 °C / min for 2 hours for sintering, and then cool with the furnace. Then cut into thin round test pieces of Φ15×2 mm;

[0053] 2)...

Embodiment 2

[0058] Synthesis of tertiary biomimetic bone tissue engineering scaffolds based on porous β-tricalcium phosphate (β-TCP) ceramics. The porosity of the porous ceramic matrix is ​​85%±5%, the diameter of the macropores is 400±50 μm, and the diameter of the interpenetrating micropores is 80±20 μm. The rest of the product preparation process and steps are the same as in Example 1. The scanning electron microscope picture of the porous β-tricalcium phosphate ceramics obtained is as follows figure 2 As shown in (a), figure 2 Middle (b) is the SEM image of collagen fibers composited in the internal pores of the porous calcium phosphate ceramic material, figure 2 Middle (c) is the SEM image of the sheet-like bone-like apatite generated on the collagen fiber template in the internal pores of the porous calcium phosphate ceramic material. The mass percentages (wt%) of calcium phosphate / collagen / bone-like apatite are: calcium phosphate ceramics-85%, collagen-14.5%, and bone-like apa...

Embodiment 3

[0060] Other conditions and processes are the same as those in Example 2, except that porous β-tricalcium phosphate ceramics with high porosity are used as the matrix to synthesize a three-level biomimetic bone tissue engineering scaffold. The ceramic porosity is 95%±5%, the macropore diameter is 500±100μm, and the interpenetrating micropore diameter is 100±20μm. The mass percentages (wt%) of the obtained calcium phosphate / collagen / bone-like apatite are: calcium phosphate ceramics-80%, collagen-19% and bone-like apatite-1%. The increase in the porosity of ceramics is conducive to the perfusion and compounding of more collagen, and is also conducive to the formation of bone-like apatite layers.

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Abstract

The invention provides a calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and a preparation method thereof. The material of the scaffold possesses components similar to natural bone tissues, and an inorganic/organic/inorganic three-level bionic bone-like netted three-dimensional structure. The preparation method comprises: customizing a calcium phosphate ceramic which possesses a bionic porous structure similar to the natural bone micropore structure, filling collagen prepared by simulated body fluid (SBF) into the porous calcium phosphate ceramic base, low-temperature aging to simulate a biomineralization process, in-situ nucleating on the collagen organic macro-molecule matrix, and self-assembly crystallizing to form a third level bone-like apatite layer structure. The composite scaffold material has bionic material components and bionic microstructure both similar to the natural bone tissues, the mechanical properties and the biological activity of the material are substantially improved, and the composite scaffold material has a wide application prospect.

Description

technical field [0001] The invention relates to a three-level bionic bone tissue engineering scaffold material compounded by calcium phosphate ceramics, collagen and bone-like apatite and a preparation method thereof, belonging to the field of biomedical materials. technical background [0002] Natural bone is a complex biocomposite material with a precise hierarchical structure. It is constructed by organic and inorganic components. The organic component is mainly Type I collagen, which endows bone tissue with elasticity and toughness. The inorganic component is mainly composed of calcium phosphate (Calcium phosphate), especially biological apatite (Biological aptite), which gives bone tissue sufficient rigidity and strength. The smallest bone unit of natural bone begins with the deposition of nanocrystalline apatite on collagen fibers. Collagen fibers provide apatite mineralization nucleation sites and control their orientation in a regularly arranged array structure. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/24A61L27/50
Inventor 周长春叶兴江樊渝江谭言飞朱向东张兴栋
Owner SICHUAN UNIV
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