83results about How to "Promote repair and regeneration" patented technology

An articular cartilage fixation device and method for repairing and regenerating diseased or injured soft tissue such as articular cartilage of the knee, hip, shoulder, and the like. A cartilage flap or cartilage repair device is retained within the chondral or osteochondral defect for a an sufficient amount of time such that the cartilage repair device can perform its function and facilitate an appropriate healing response. A plurality of biocompatible anchors are attached to a plurality of flexible members and are shaped to seat into the tissue beneath or near the defect such that the cartilage flap is retained within the defect.

The invention relates to a preparation method of three-layer lead tissue regenerating velum with gradient, belonging to macromolecule biomedical material field. The velum is a three-layer composite velum made from natural biological material, degradable polymer and nanometer hydroxyapatite as raw materials. The method comprises steps of dissolving mixture of the natural biological material, degradable polymer and nanometer hydroxyapatite with different proportions in a special solvent; preparing the natural biological material/degradable polymer/nanometer hydroxyapatite three-layer gradient composite velum through static spinning layer by layer; and using pure natural biological material to modify surface of the velum by means of static self-assembly layer by layer. The velum has excellentbiological compatibility and mechanical property; one side of the velum is crude and loose, so the side can face to a defect area and is helpful for bones to absorb and grow and for blood coagulationblocks to stabilize; the other side is smooth and compact, so the side can face to surrounding tissue to prevent connective tissues from entering the defect area.

The invention provides a calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and a preparation method thereof. The material of the scaffold possesses components similar to natural bone tissues, and an inorganic/organic/inorganic three-level bionic bone-like netted three-dimensional structure. The preparation method comprises: customizing a calcium phosphate ceramic which possesses a bionic porous structure similar to the natural bone micropore structure, filling collagen prepared by simulated body fluid (SBF) into the porous calcium phosphate ceramic base, low-temperature aging to simulate a biomineralization process, in-situ nucleating on the collagen organic macro-molecule matrix, and self-assembly crystallizing to form a third level bone-like apatite layer structure. The composite scaffold material has bionic material components and bionic microstructure both similar to the natural bone tissues, the mechanical properties and the biological activity of the material are substantially improved, and the composite scaffold material has a wide application prospect.

The invention provides a recombinant human XVII type collagen. The collagen is composed of amino acid sequence shown as (A)n or comprises an amino acid sequence shown as (A)n, wherein A is a sequence shown as SEQ ID NO.2 or a sequence obtained by carrying out amino acid modification to a certain extent on the basis of SEQ ID NO.2 or a sequence having more than 80% of homology with SEQ ID NO.2; n is an integer greater than or equal to 1; and each A is the same or different and is serially repeated by taking A as a basic unit, and each same or different A is directly connected through a peptide bond. It is verified that the collagen not only can realize the efficient secretion soluble expression of the human XVII type collagen in the pichia pastoris which is an eukaryotic host cell, but also can realize the high-efficiency secretion soluble expression of the human XVII type collagen; in addition, the collagen also has more excellent cell adhesion activity, cell migration promotion activity and excellent tissue regeneration and biological activity of promoting hair follicle repair and regeneration than commercial natural human collagen, and can be industrially produced.

The invention belongs to the technical field of cosmetics, and in particular relates to a composition with functions of skin repairing and age defying, and a preparation method of the composition. Theskin care composition with the functions of skin repairing and age defying is prepared from the following components in parts by mass: 33-47 parts of chitosan oligosaccharide, 10-20 parts of acylatedchitosan oligosaccharide, 20-30 parts of chitosan, 22-38 parts of carboxymethyl chitosan, 10-15 parts of beta-glucan and 3-6 parts of schizophyllan. The composition with the functions of skin repairing and age defying is safe to use, mild in properties and free from skin irritation, and does not cause skin allergy. Furthermore, the composition also can replenish skin cell nutrients so as to achieve the effects of deeply repairing, promoting cell metabolism and activating cell vitality; the composition also has better anti-bacterial, moisturizing and anti-aging functions for the skin.

The invention discloses a preparation method of a polyhydroxyalkanoate/polydopamine composite electrospinning film and the electrospinning film. According to the method, the polyhydroxyalkanoate electrospinning film is prepared by means of an electrospinning technology, and polydopamine (PDA) is grafted onto the surface of a polyhydroxyalkanoate fiber skeleton through chemical modification to prepare the polyhydroxyalkanoate/polydopamine composite electrospinning film. The polyhydroxyalkanoate/polydopamine composite electrospinning film prepared by means of the method has the advantages of great adhesion prevention performance, high biocompatibility and excellent mechanical properties and has a wide application prospect in the biomedical fields of tissue repairing, prevention of tissue adhesion and the like.

The invention is directed to a composition and associated method for the treatment of musculoskeletal related disorders and diseases. In particular, the present invention provides a class of citrate compounds that can be used to for the treatment of diseases/disorders characterized by the degeneration of musculoskeletal tissues including menisci, bone, articular cartilage, and soft tissues. Examples of suitable citrate and citrate analog compounds include citrates having the following base formula (I):

where X may be one of the following:

wherein Y, Y′, and Y″ are independently a citrate moiety, O, O—Y, OH, NH, NH—Y, R and R—Y, with R being an alkyl, alkyene, ester, aryl, or phenyl group having from 1 to 6 carbon atoms.

The invention discloses a hydrogel/fibroin nanofiber/neural stem cell integrated transplant capable of promoting VEGF secretion as well as a preparation method and application thereof, and belongs tothe technical field of biological tissue medical engineering. The method comprises the following step: polysaccharide-based hydrogel/neural stem cell gel are adhered to a fibroin nanofiber scaffold through dopamine to form a polysaccharide-based hydrogel/fibroin nanofiber/neural stem cell integrated transplant, wherein the polysaccharide-based hydrogel is a self-healing injectable polysaccharide-based hydrogel. According to the invention, the polysaccharide-based hydrogel embedded with the neural stem cells is adhered to a gap between fibroin nanofiber scaffolds through dopamine to construct the polysaccharide-based hydrogel/fibroin nanofiber/neural stem cells integrated transplant, so that the cells can be promoted to secrete VEGF and fill the defect part, and provide required microenvironment for adhesion, proliferation and directional differentiation of the neural stem cells into neurons, and the neural stem cells can be applied to the field of treatment of cerebral ischemia injuryand promotion of neural stem cell amplification and differentiation.

The invention discloses a composition, which is prepared from the following raw materials: a mesenchymal stem cell active factor compound, a hydrolyzed placenta extract, hyaluronic acid, collagen, water and an auxiliary reagent. The mesenchymal stem cell active factor compound is prepared from a cell culture supernatant of mesenchymal stem cells and a cell lysis solution through an ultrafiltrationmethod. The hydrolyzed placenta extract is prepared from placenta tissues through decellularization and enzyme digestion methods. The content of active factors in the composition is 0.1-50 micrograms/ml. The invention also discloses a preparation method and application of the above composition. According to the embodiment of the invention, stem cell secretions and accellular placenta tissues areapplied such that the risk of anaphylactic reaction possibly caused by direct use of stem cells is reduced, and effective regulatory factors are all from cell secretions and have higher activity thanrecombinant active factors.

The invention relates to the technical field of cell exosome preparation, in particular to a dental pulp stem cell exosome and a preparation method and application thereof. The preparation method of the dental pulp stem cell exosome sequentially comprises the following steps: mixing a dental pulp mesenchymal cell suspension and a low-melting-point agarose solution to obtain a mixture, then inoculating the mixture into a culture container, and carrying out pre-culture and induction culture to obtain a supernatant containing the exosome; and extracting the exosome in the supernate. The preparation method of the scheme solves the technical problem that the treatment effect of the exosome obtained by the existing technical method is limited. In the exosome obtained by the scheme, the expression quantity of miRNAs such as miR-302a/b/c/d family and miR-24-3p is remarkably improved, and the exosome obtained by using the three-dimensional cultured dental pulp mesenchymal cells has a relatively remarkable treatment application potential.

The invention relates to a microecological preparation for poultry and belongs to the field of veterinary drug. The microecological preparation is prepared from clostridium butyricum, lactobacillus acidophilus, beer yeast, bacillus subtilis and other probiotics, auxiliary materials are added, and the microecological preparation is formed through clathration of clathrate compounds. The microecological preparation can adjust the gastrointestinal flora microenvironment of meat and egg poultry, and the disease incidence of diarrhea and other gastrointestinal diseases is reduced. The absorption and conversion capacity of a body is enhanced, the feed utilizing rate is increased, the content of ammonia in faeces is reduced, the condition of a chicken house is improved, and therefore the incidence rate of respiratory diseases of poultry is reduced.

The invention provides a tantalum-doped hydroxyapatite coating bone implantation material and a preparation method thereof. The tantalum-doped hydroxyapatite coating bone implantation material comprises a metal matrix and a composite coating, wherein the composite coating comprises tantalum and hydroxyapatite, and the weight ratio of tantalum in the composite coating is 60%-90%; and the porosity of the composite coating is 18%-22%. Mixed powder of tantalum and hydroxyapatite is melted and is sprayed to metal matrix by virtue of an atmosphere plasma spraying method; the spraying conditions are as follows: the spraying power is 20KW-30KW, the powder feeding velocity is 10g/min-15g/min, the argon feeding quantity is 35L/min-55L/min, and the helium feeding quantity is 8L/min-20L/min. By virtue of the preparation method, the binding strength between the coating and the matrix is enhanced, the porosity of the coating is improved, and the material has good biological activity and is capable of promoting the adhesion and proliferation of cells and bone-formation differentiation and is beneficial to the restoring and regeneration of bone tissues.

The invention provides a method for purifying pluripotent vascular progenitor cells from a perinatal tissue. The method comprises the steps of pretreatment and acquisition, wherein in the acquisitionstep, immunomagnetic beads of two different ligands are used for marking and purifying the perinatal tissue cells, the first immunomagnetic beads are used for marking firstly, through positive expression, non-pluripotent vascular progenitor cells are recognized, and impurity cells are removed to obtain a high-concentration cell population containing the pluripotent vascular progenitor cells, thenthe impurity-removed perinatal tissue cells are marked through the second immunomagnetic beads, and recognition is carried out through positive expression to obtain the pluripotent vascular progenitorcells. The pluripotent vascular progenitor cells prepared through the method have phenotypes similar to phenotypes of bone marrow mesenchymal stem cells and perivascular cells at the same time, havethe same mesoderm cell differentiation function as the bone marrow mesenchymal stem cells, further have the functions of differentiating into mature vascular cells and promoting cardiovascular systemrepair and regeneration, and can maintain the cell morphology and the amplification capability.

The invention provides photo-thermal-ion synergistic antibacterial hydrogel as well as a preparation method and application thereof and relates to a polydopamine/copper-doped bioactive ceramic composition. The polydopamine/copper-doped bioactive ceramic composition comprises raw materials as follows: modified polyglutamic acid polymer aqueous solution grafted and modified by dopamine and adipic dihydrazide as well as copper-doped bioactive ceramic powder, wherein the mass percent concentration of the modified polyglutamic acid polymer aqueous solution is 2.5%-10%, and the addition amount of the copper-doped bioactive ceramic powder is 0.5%-5% of the mass of the modified polyglutamic acid polymer aqueous solution.

The medicine for treating aseptic necrosis of femur head cooperating with high pressure oxygen therapy is prepared with Dangshen, white atractylodes rhizome, tuckahoe, cassia twig, licorice and other 19 kinds of Chinese medicinal materials and through stoving, crushing, sterilization, and produced into bolus. The medicine of the present invention has high curative effect and high effective rate, and no side effect.

The invention relates to the technical field of cosmetics and discloses bandage type body wrap and a body skin care method. The bandage type body wrap comprises a body wrap substrate and essence adsorbed on the body wrap substrate, wherein the body wrap substrate is a bandage; the essence contains active components and base components, and the active components comprise a dendrobium officinale extract, superoxide dismutase, squalane and a licorice extract. The body skin care method comprises the step of winding the bandage type body wrap around a body part. The bandage type body wrap can be used at any part of the body, is convenient to use, high in universality and tightly attached, facilitates nutrition uptake, has no skin irritation and can maintain skin moisture effectively, promote repair and regeneration, delay skin senescence and recover skin elasticity.

The invention relates to the technical field of rhinitis preparation and specifically relates to a rhinitis ointment and a preparation method thereof. The rhinitis ointment is prepared from the following raw materials in parts by weight: 10-20 parts of flos lonicerae, 8-20 parts of flos magnoliae, 5-8 parts of borneol, 5-10 parts of propolis liquid, 5-8 parts of vitamin A, 2-7 parts of vitamin B1,2-7 parts of vitamin B2, 5-8 parts of vitamin C, 5-8 parts of vitamin E, 2-5 parts of zinc oxide, 15-25 parts of glycerin, 20-25 parts of vaseline, 20-25 parts of wool fat, 10-15 parts of olive oil and 3-5 parts of azone. According to the rhinitis ointment and the preparation method thereof provided by the invention, the nasal diseases are effectively cleaned, cared and treated; the prepared rhinitis ointment has the advantages of convenience in carrying and using, no hormone medicine, short onset time, good long-term maintenance effect and no side effect, solves the problem of existing rhinitis medicine that the curative effect is very little and the rhinitis easily relapses, and brings good news to rhinitis patients.

The invention relates to the technical field of nutrition food, in particular to organic black millet nutrition food. The organic black millet nutrition food comprises 15-18 parts of black millet, 2.5-3 parts of soybeans and 2.5-3 parts of walnuts; and after the black millet and the soybeans are washed, dried in the air, evaporated and baked for 10 minutes, the black millet, the soybeans and the dry walnuts are mixed according to the proportioning and are ground into 100-120 meshes, and finally the ground mixed powder is packaged. The organic black millet nutrition food provided by the invention contains nutrition ingredients such as abundant protein, carrot elements, vitamins, mineral substances, calcium, iron, zinc, selenium and the like and has the advantages of balancing the physiological function of human bodies, promoting the metabolism, reinforcing the immunity and playing dietary therapy role in comprehensively replenishing the nutrition of the human bodies. The organic black millet nutrition food contains low glucose, low fat, high protein and high vitamins and is ideal for diabetics. The beta-carotenes have good effects on reparative regeneration and exertion of normal functions of epithelial cells of digestive tracts.

The invention relates to a veterinary medicine, in particular to a medicine composition for preventing and treating the laying hen fatty liver syndrome and a preparation method thereof. The medicine composition is prepared from traditional Chinese medicine components and western medicine components according to the weight ratio of 2:1. The traditional Chinese medicine components include, by weight, 25-35 parts of Radix Salviae Miltiorrhizae, 18-22 parts of Schisandrachinensls (Turcz.) Baill, 8-12 parts of radix angelica sinensis, 8-12 parts of radix astragali, 8-12 parts of radix bupleuri, 8-12 parts of radix isatidis and 8-12 parts of liquorice roots. The western medicine components include, by weight, 80-120 parts of choline chloride, 100-150 parts of methionine, 1.8-2.2 parts of vitamin E, 0.001-0.0015 part of vitamin B, 80-120 parts of inositol and 150-200 parts of glucose. Western medicine compounds and traditional Chinese medicine such as Radix Salviae Miltiorrhizae are used in cooperation, and the treatment effect is improved.

The invention relates to an external medicament for treating cervical erosion, which comprises the following Chinese herbal medicines: 40 to 60 grams of fructus cnidii, 40 to 60 grams of kuh-seng, 2o to 40 grams of phellodendron amurense, 20 to 40 grams of broom cypress fruit, 2o to 40 grams of nutgall, 5o to 80 grams of mother-of-pearl, 20 to 40 grams of coptis, 20 to 40 grams of rhubarb, 40 to 60 grams of peach kernel, 10 to 30 grams of angelica dahurica, 20 to 40 grams of white paeony root, and 20 to 40 of fructus xanthil. The external medicament is an external lotion prepared by the Chinese herbal medicines, is directly applied to the focus at the pudendum, remarkably improves the internal environment of vagina, causes the local necrosis tissue to drop off, promotes the healing and regeneration of the cervical squamous tissue, and has no toxic and side effects and good treatment effect.