Method of purifying pluripotent vascular progenitor cells from perinatal tissue

A tissue cell and perinatal technology, applied in the field of biomedical research, can solve the problems of good repeatability and fast purification of multipotent vascular progenitor cells, achieve good expansion potential, and promote the effect of cardiovascular system repair
CN112159790AActive Publication Date: 2021-01-01至仁生命科技(天津)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
至仁生命科技(天津)有限公司
Publication Date
2021-01-01

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Abstract

The invention provides a method for purifying pluripotent vascular progenitor cells from a perinatal tissue. The method comprises the steps of pretreatment and acquisition, wherein in the acquisitionstep, immunomagnetic beads of two different ligands are used for marking and purifying the perinatal tissue cells, the first immunomagnetic beads are used for marking firstly, through positive expression, non-pluripotent vascular progenitor cells are recognized, and impurity cells are removed to obtain a high-concentration cell population containing the pluripotent vascular progenitor cells, thenthe impurity-removed perinatal tissue cells are marked through the second immunomagnetic beads, and recognition is carried out through positive expression to obtain the pluripotent vascular progenitorcells. The pluripotent vascular progenitor cells prepared through the method have phenotypes similar to phenotypes of bone marrow mesenchymal stem cells and perivascular cells at the same time, havethe same mesoderm cell differentiation function as the bone marrow mesenchymal stem cells, further have the functions of differentiating into mature vascular cells and promoting cardiovascular systemrepair and regeneration, and can maintain the cell morphology and the amplification capability.
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Description

technical field

[0001] The invention belongs to the field of biomedical research, in particular to a method for purifying and obtaining multipotent vascular progenitor cells from perinatal tissues. Background technique

[0002] Perinatal tissue refers to the accessory organs and tissues related to fetal development produced during the period from 28 weeks of pregnancy to the birth of the baby, mainly including cord blood, umbilical cord and placenta. The perinatal tissue after the fetus is delivered at full term has a small chronological age and a low gene mutation rate. It is usually discarded as biological waste and can be obtained without any risk to the fetus and mother. The acquisition method will not cause harm to the human body, etc. advantage. Perinatal tissue has become the focus of attention in the field of regenerative medicine because it contains a variety of stem cells and specific tissue progenitor cell subsets, and has many advantages such as abundant resourc...

Claims

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