19 results about "Tissue cell" patented technology

The utility model discloses a device capable of simultaneously carrying out tissue cell collection and confocal imaging, which comprises a workbench and a puncture needle, one side of the workbench is provided with a collection detection mechanism, the collection detection mechanism comprises a transverse plate, one side of the transverse plate is fixedly connected with a hollow block, and the hollow block is fixedly connected with the puncture needle. An electric telescopic rod is fixedly connected to the front face of the hollow block, a connecting frame is fixedly connected to one side of the electric telescopic rod, and a sealing plate is fixedly connected to one side of the connecting frame. By arranging a collecting and detecting mechanism, starting an electric telescopic rod, driving a connecting frame to move by the electric telescopic rod, and driving a sealing plate to move by the connecting frame, negative pressure suction work is performed, cells are sucked into an inner cavity of a hollow block, and after part of the cells are sucked, the electric telescopic rod can be controlled to contract, so that the cells are pushed into an inner cavity of a fixed pipe through the sealing plate; cells are discharged to the top of the bearing plate through the fixing pipe, meanwhile, the bearing plate is moved to a detection position, and detection work is conducted through the detection probe.

The invention discloses a composite therapeutic apparatus for synchronously treating closed fracture and complications thereof, and relates to the field of therapeutic apparatuses, the composite therapeutic apparatus comprises an upper layer main shell, a lower layer main shell and a bottom shell, the bottom shell is internally provided with an automatic locking unit for rapidly locking the upper layer main shell and the lower layer main shell; a pressing unit; the treatment structure is electrically connected with the control mainboard; the intermediate-frequency treatment unit and the LIPUS treatment unit are integrated on one device, the purpose of simultaneously treating the fracture affected part by jointly using LIPUS and intermediate-frequency electrical stimulation is achieved, meanwhile, the functions of easing pain, eliminating inflammation and promoting tissue and cell regeneration of the affected part are achieved, and the application of the patent product is not limited to fracture injuries, but also limited to bone fracture injuries. The device can also be applied to treatment of closed skeletal muscle movement system soft tissue injury, is small in size and flexible in deployment, has good sanitary conditions and reusability, and can provide good treatment capacity for long bone fracture patients in a bandaging and fixing state.

A type of under-eye implant includes two mirror-symmetrical implant units. Each implant unit is arc-shaped, tapering at both ends and thickening in the middle along its length. All edges and surfaces are smoothly transitioned through rounded surfaces. The arc trajectory of the implant unit matches the natural contour of the lower eyelid from the inner corner to the outer corner, with the radius of curvature of the inner segment near the inner corner being larger than that of the outer segment near the outer corner. Each implant unit has a through-hole at each end. The implant is made of expanded polytetrafluoroethylene (ePTFE), whose microporous structure induces tissue cell ingrowth after implantation, preventing implant displacement and light transmission, and demonstrating good biocompatibility.

The invention provides a dental pulp regeneration structure based on a piezoelectric intelligent material and a preparation method of the dental pulp regeneration structure, and relates to the technical field of dental pulp regeneration, the preparation method of the dental pulp regeneration structure based on the piezoelectric intelligent material comprises the following steps: mixing a biodegradable polymer and piezoelectric ceramic in chloroform to form uniform slurry, the method comprises the following steps: constructing a three-dimensional porous scaffold with periodically arranged pores by adopting a 4D printing method, wherein the Poisson ratio of the three-dimensional porous scaffold is-0.3 to-0.8; and a piezoelectric material film is attached to the surface of the three-dimensional porous bracket. The tissue scaffold forming the three-dimensional space complex has a negative Poisson's ratio superstructure design, the energy absorption effect can be remarkably improved, the piezoelectric property can be enhanced, the differentiation efficiency of tissue cell nerves can be improved through stronger electrical stimulation, and dental pulp regeneration is promoted.

The degradable stent is of a hollow tubular structure with the two ends open and comprises a main tube body and retention flanges at the two ends of the main tube body, the main tube body comprises a plurality of coaxially-nested sub-tube bodies, annular gaps are formed between the adjacent sub-tube bodies, the two ends of any sub-tube body are connected with the retention flanges, and a plurality of holes are formed in the tube wall of each sub-tube body; and in the radial direction of the main pipe body, the porosity of the pipe wall of the sub pipe body is gradually increased from the central axis to the periphery. The main tube body is arranged to be a plurality of coaxially nested sub-tube bodies with gradually increased porosity, an outer-layer tissue migration guiding channel is formed, the sub-tube bodies on the outermost layer of the stent preferentially induce rapid migration and colonization of autologous tissues, and a gradual mechanical supporting environment is provided for deep tissue cell ingrowth along with the sequential reduction of the porosity of the inner layer. When autologous cells construct a complete cavity tissue structure, each layer of material of the stent is gradually degraded and absorbed according to a preset gradient, and finally a natural cavity with a physiological function is formed at an implantation part, so that the risk of a secondary operation is avoided.

The invention discloses a method for obtaining human sclerosing bone tissue cells suitable for full transcriptome sequencing, comprising the following steps: tissue cleaning and pretreatment: placing human sclerosing bone tissue in a centrifuge tube, soaking it in DPBS, inverting the centrifuge tube to clean the human sclerosing bone tissue, and incubating it with precooled BL buffer for pretreatment; tissue treatment and enzymatic hydrolysis: passing the incubated human sclerosing bone tissue and BL buffer through a cell screen, and rinsing the tissue 2-3 times with precooled PBS; transferring the human sclerosing bone tissue on the cell screen to a culture dish, adding BE solution, cutting the tissue into small pieces, and adding more BE solution; placing the culture dish in a 37°C constant temperature incubator, performing enzymatic hydrolysis for 15 minutes, and shaking the culture dish every 5 minutes; after the enzymatic hydrolysis is completed, transferring the enzymatic hydrolysis solution to a new centrifuge tube using a pipette; cell separation and collection: filtering the enzymatic hydrolysis solution using a cell screen, rinsing the cell screen with precooled DPBS containing 0.1% (m / v) BSA; centrifuging the filtrate in a horizontal centrifuge to collect cells.

The utility model provides a primary tissue cell suspension preparation device which comprises a suspension preparation instrument, a preparation bin is arranged at the inner end of the suspension preparation instrument, a suspension cylinder is arranged at the inner end of the preparation bin, an embedded ring groove is formed in the inner end of the suspension cylinder, a centrifugal counterweight cylinder is arranged at the inner end of the embedded ring groove, and a positioning spring is arranged at the outer end of the centrifugal counterweight cylinder. An embedded groove is formed in the inner end of the suspension cylinder, a positioning shaft is arranged at the inner end of the embedded groove, a positioning rod is arranged at the outer end of the positioning shaft, a hook plate is arranged at the upper end of the positioning rod, an outer ring groove is formed in the outer end of the hook plate, a silica gel ring is arranged at the inner end of the outer ring groove, and a suspension pipe is arranged at the inner end of the suspension cylinder. According to the cell suspension preparation device, the problems that in the design of cell suspension preparation, when a suspension tube is centrifugally rotated, some suspension cylinders are not provided with the suspension tube in a preparation bin, so that the inertia force borne by the vacant suspension cylinders is inconsistent with the inertia force borne by the suspension cylinders containing the suspension tube in the centrifugal movement process, and the centrifugal effect is easily influenced are solved.

The invention provides a puncture force and shape sensing puncture needle and method based on fiber bragg grating sensing. The puncture needle and method are used for pulmonary nodule puncture biopsy under a bronchoscope. The puncture needle provided by the invention comprises a needle body assembly and a sensing assembly, the needle body assembly is connected with the sensing assembly to form a puncture needle body with a sensing function; the structure is ingenious, the annular groove structure is arranged on the puncture needle and used for collecting tissue cell samples, the FBG optical fiber is integrated on the puncture needle through the structural design, the shape sensing function and the puncture force sensing function of the puncture needle are achieved, more puncture information is provided for an operation doctor, and the puncture precision is improved.

The invention relates to a plugging structure convenient for cell creeping attachment, which comprises a plugging body, the plugging body comprises a contraction section and an outer disc section, and the contraction section and the outer disc section are connected with each other; in the unfolded state, the outer diameter of the contraction section is smaller than that of the outer disc section, the outer surface of the outer disc section comprises a circular outer disc face and an annular outer circumferential face, the cross section of the outer circumferential face is semicircular, the outer edge of the outer disc face is connected with the first edge of the outer circumferential face, and the outer edge of the outer disc face is connected with the second edge of the outer circumferential face. The first edge of the peripheral surface is connected with one end of the contraction section, the second edge of the peripheral surface is connected with one end of the contraction section, the first edge and the second edge are circular, the first edge is far away from the contraction section compared with the second edge, and a hydrogel layer is formed on the peripheral surface. And the hydrogel layer is formed on the peripheral surface, so that the creeping attachment of tissue cells is facilitated, and the endothelialization process is accelerated.

The application discloses a modeling method and system for dynamic stress stimulation regulation of bone healing, which is applied to the technical field of bone healing and comprises the following steps: establishing a geometric model of a fracture site; performing mesh division on the geometric model to generate a finite element model of bone and callus; assigning material properties at an initial time in the callus; applying a dynamic load constraint to the top end of cortical bone based on the finite element model to establish a bone healing model; calculating new material properties in tissue cells, judging whether the new material properties reach a preset healing standard through the bone healing model; if the preset healing standard is reached, the simulation is completed, otherwise, iterative simulation is performed through dynamic adjustment of mechanical parameters until the preset healing standard is reached, and finally, bone healing data is output. Through the bone healing model, loading parameters are adjusted in real time according to simulation data, and the accuracy and reliability of bone healing simulation are improved.

The invention relates to the technical field of cervix uteri sampling in the obstetrics and gynecology department, in particular to a cervix uteri scraping and brushing sampling device for the obstetrics and gynecology department, which comprises a fixed tube and a handle fixedly connected to the fixed tube, and further comprises an internal thread frame placed on the inner side of a cylinder body, a driving assembly is arranged between the internal thread frame and the cylinder body, and the handle is fixedly connected to the fixed tube. The internal thread frame is driven to move. Firstly, the limiting frame supports and limits the inside of the cervix uteri, the movable column drives the brush plate to move to make contact with the inside of the cervix uteri through the driving base and the connecting rod, then the brush plate rotates to brush down tissue cells in the cervix uteri to complete sampling, the brush plate moves inwards to be reset and retracted, and the brush plate drives the brushed-down tissue cells to move into the limiting frame; the device can be taken out from the cervix uteri and the vagina of the patient by taking out the tissue cells, the brushed tissue cells are located in the limiting frame, the limiting frame can block other parts in the body of the patient, independence of the brushed tissue cells is guaranteed, it is guaranteed that the tissue cells do not interfere with other parts, and therefore the sampling effect is improved.

The utility model discloses a low-temperature plasma surgery electrode knife head convenient for fine operation, which comprises a knife handle, the front side of the knife handle is connected with a knife head main body, one end of the knife head main body is provided with a blood coagulation working surface, and a plurality of micro convex ball points are uniformly distributed on the upper side surface and the lower side surface of the blood coagulation working surface. According to the low-temperature plasma operation electrode knife head, the knife handle, the knife head body, the insulating coating, the cutting working area, the side knife face, the front knife face, the knife head cutting edge, the bottom knife face and the top knife face are arranged, the area for cutting work is designed to be small, and the contact area between the electrode cutting working area and tissue cells of a patient is reduced; by arranging the blood coagulation working face and the micro-convex ball points, when high-frequency current is generated in the electrode, due to the fact that the surface area of the smooth surface is increased, the skin effect of the high-frequency current is formed, charges are concentrated on the surfaces of the micro-convex ball points, energy is focused through the vertexes of the micro-convex ball points, the energy density of the contact position with local tissue is increased, tissue blood coagulation is faster, and the blood coagulation efficiency is improved. The blood coagulation efficiency is higher.

The invention provides an oral cavity tissue sampling and detecting device for stomatology department nursing, and belongs to the technical field of medical instruments. The device comprises a machine body and a swab, a hollow connecting pipe is fixedly connected to the outer surface of the right end of the swab, and a mounting pipe used for clamping and fixing the connecting pipe is arranged on the left side of the machine body; the surface of the swab is provided with meshes for a liquid sample to permeate, an inner cavity of the swab is communicated with the connecting tube, a negative pressure generation assembly is arranged in the machine body, and the negative pressure generation assembly comprises a sealing sleeve capable of being pressed in a reciprocating manner, a cavity sealed by the sealing sleeve and a drainage tube communicated with the cavity and the connecting tube; when the sealing sleeve is pressed and released, negative pressure can be generated in the cavity, the connecting tube communicated with the cavity and the swab, so that a liquid sample in the oral cavity is sucked in through the meshes and temporarily stored. According to the scheme, the swab is used for dipping tissue cells in the oral cavity, saliva or wound secretions in the oral cavity can be sucked into the connecting tube through the meshes in the surface of the swab, the application range is widened, and the detection precision is improved.

Techniques for High-Frequency Irreversible Electroporation (HFIRE) using a single-pole tine-style internal device communicating with an external surface electrode are described. In an embodiment, a system for ablating tissue cells in a treatment region of a patient's body by irreversible electroporation without thermally damaging the tissue cells is described. The system includes at least one single-pole electrode probe for insertion into the treatment region, the single-pole electrode probe including one or more tines. The system further includes at least one external surface electrode for placement outside the patient's body and configured to complete a circuit with the single-pole electrode probe. The system also includes a control device for controlling HFIRE pulses to the single-pole tine-style electrode and the skin-surface electrode for the delivery of electric energy to the treatment region. Other embodiments are described and claimed.

In order to realize the absorption of mask liquid by skin and improve the absorption efficiency of mask solution by skin, the invention designs a structure of a photomagnetic mask, high-frequency laser is irradiated on skin tissues, and a cell magnetic field is generated in tissue cells through induction; under the action of a cell magnetic field, the absorption of capillary pores of facial skin and skin attachments to liquid is promoted. Aiming at edema lesions of different facial skins and pigmentation of epidermis, photomagnetic effect regions with different wavelengths and frequencies are divided, and partitioned treatment of the facial skins and absorption of deposited pigments are realized.

The application provides a portable non-closed oxygen inhalation mask, and relates to the field of medical auxiliary devices; the oxygen inhalation mask comprises: a flexible mask used for being worn on the head and face of a user, which is provided with a connecting port at a position opposite to the mouth and nose of the user; a supporting framework is arranged in the flexible mask; a cup cover is arranged on the side of the flexible mask away from the face of the user, and the open end of the cup cover is in sealing connection with the connecting port; an air inlet for being connected with an oxygen supply pipe is arranged on the side wall or the bottom of the cup cover; wherein the volume of the cup cover can be adjusted, and the overall weight is relatively light to avoid sliding. The application can improve the oxygen concentration around the mouth and nose of the user to improve the oxygen utilization rate; reduce the damage of the oxygen flow to the nasal mucosa and the face, thereby improving the oxygen inhalation comfort; improve the carbon dioxide concentration around the mouth and nose, maintain the relatively normal blood carbon dioxide partial pressure through carbon dioxide reabsorption to promote the red blood cells to release oxygen to tissue cells, and improve the internal respiration efficiency of each organ of the body and the effect of oxygen inhalation treatment.

The invention relates to the technical field of airway stents, in particular to a biodegradable airway stent which comprises a main stent structure, and the main stent structure is composed of a radial frame and a transverse frame. The inner fixing frame is spirally mounted and combined with the spiral mounting frame in the outer fixing frame through the spiral mounting strip, the friction force between the outer fixing frame and the inner fixing frame can be increased through spiral textures, the risk of displacement is reduced, a layer of degradable biological adhesive is coated between the spiral mounting strip and the spiral mounting frame, and the biological adhesive can be gradually decomposed in the degradation process; and the surface of the outer fixing frame is uniformly distributed with growing and attaching micropores, so that the ingrowth of tissue cells and the exchange of nutrient substances are facilitated, the stress on the outer fixing frame can be dispersed in multiple directions through the micropore structures, and through the spiral combination and the micropore structure combination, the stress on the outer fixing frame can be dispersed in multiple directions. And stress concentration can be effectively reduced.

The invention provides a facial soft tissue defect repair device based on a bio-absorbable stent, and belongs to the technical field of medical repair, the facial soft tissue defect repair device comprises a 3D printing bio-absorbable stent and a growth factor slow-release coating; according to the 3D printing biological absorbable stent, a biodegradable polymer material without an inorganic material is adopted, a porous stent matched with a facial defect area of a patient is prepared through a 3D printing technology, and the porous stent is of a bionic micro-nano structure simulating a natural facial soft tissue extracellular matrix; the mechanical support and the three-dimensional space for the growth of facial soft tissues are provided. The stent is made of a biodegradable polymer without inorganic materials, local inflammation or foreign body reaction possibly caused by inorganic components in the face soft tissue environment can be avoided, the stent does not need to be taken out through a secondary operation due to the biodegradable characteristic, and the pain of a patient is relieved. The device promotes tissue regeneration by precisely adapting to the defect part, overcomes the defects of a traditional method, and has remarkable advantages.

Cellular therapy infusion devices for the delivery of media including cellular therapies to a patient's tissue, cells, and / or blood include a barrel, a plunger that fits within the barrel, a syringe shaft, and a pressure relief system configured to achieve and / or maintain a desired level of pressure and / or force within the device so that cellular behavior and / or viability is not adversely impacted by the mechanical forces exerted thereon via depressing the plunger into the barrel so the cellular therapy media may be pushed from the barrel into a patient delivery device for administration to the patient. The cellular therapy infusion devices may cooperate with and / or fit into cellular therapy infusion systems designed to create and / or maintain preferred conditions for the cellular therapy media stored in the barrel and automatically administer the cellular therapy media from the cellular therapy infusion device to the patient in a steady, regulated, and / or preferred manner.