The invention relates to an extraction method and application of orchid root
polysaccharide, which can effectively extract orchid root
polysaccharide GLHG0 from orchid roots and realize application in preparation of medicines for treating
lung injury. The method comprises the following steps:
cutting orchid roots into sections, performing
reflux extraction, and concentrating; centrifuging to obtain a precipitate, adding water for suspension after deproteinization treatment, adding a sevage
reagent, magnetically stirring, standing, layering, taking an upper-layer solution, concentrating under reduced pressure, adding H2O2, adjusting the pH value of a
reaction system by using a NaOH solution, decolorizing, dialyzing by using a
semipermeable membrane, and freeze-
drying to obtain
powder; dissolving with deionized water, centrifuging, taking supernate, and filtering; carrying out
gradient elution, detecting and identifying, drawing an
elution curve, carrying out vacuum concentration, dialyzing, freeze-
drying, selecting a water component
polysaccharide, preparing a polysaccharide solution, and purifying and separating. The raw materials are rich, the preparation method is simple and easy to operate,
technical support is provided for development of novel medicines for treating
lung injury, and practical application and popularization value is achieved.