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Personalized bionic composite structure as well as preparation method of composite structure and method for drug screening by using composite structure

A technology of biomimetic composite structures and drugs, applied in biochemical equipment and methods, measurement/testing of microorganisms, animal cells, etc., can solve problems such as inability to fully simulate the real structure of multi-cells

Inactive Publication Date: 2014-04-30
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing tissue engineering cell / scaffold composite structures can express the characteristics of tissues or organs in a certain dimension in vitro, but they cannot fully simulate the real structure of multicellular cells containing vascular system

Method used

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  • Personalized bionic composite structure as well as preparation method of composite structure and method for drug screening by using composite structure
  • Personalized bionic composite structure as well as preparation method of composite structure and method for drug screening by using composite structure
  • Personalized bionic composite structure as well as preparation method of composite structure and method for drug screening by using composite structure

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preparation example Construction

[0034] The preparation method of a personalized bionic composite structure provided by the present invention comprises the following steps: 1) designing a three-dimensional model of a branch vessel stent, and dividing into two groups to imitate arterial vessels and venous vessels respectively; The hydrogel of tissue cells, the prepared hydrogel containing vascular seed cells and the prepared synthetic polymer solution are loaded into different nozzles of the composite multi-nozzle 3D printing device; 3) Preparation of branch vascular scaffolds: according to step 1) The three-dimensional model of the composite multi-nozzle 3D printing equipment is used to extrude the synthetic polymer solution, and freeze-dried to remove the organic solvent, or the melt-extruded synthetic polymer material is obtained to obtain the branch vessel stent; 4) Preparation of functional tissue area: use the composite multi-nozzle The 3D printing equipment prints the prepared hydrogel containing tissue ...

Embodiment 1

[0039] Example 1: Preparation of a personalized bionic vascularized liver tissue and its application in hepatitis drug screening

[0040] 1) Preparation of cell-containing hydrogel: extract rat adipose stem cells (ADSC) and hepatocytes (Hep), and prepare cell suspension after ADSC is cultured and passaged; mix natural polymer powder with (DMEM, dulbecco's modified eagle medium) The culture solution is mixed to obtain a gelatin solution with a mass volume concentration of 10%, a sodium alginate solution with a mass volume concentration of 2%, and a fibrinogen solution with a mass volume concentration of 1%. The above gelatin solution, sodium alginate solution and fiber The volume ratio of the original protein solution was mixed at 2:2:1 to obtain a natural polymer solution for use; the ADSC and Hep suspensions were centrifuged and mixed with the natural polymer solution respectively to obtain a cell concentration of 1×10 6 Individual / mL natural polymer solution containing ADSC,...

Embodiment 2

[0045] Example 2: Preparation of a personalized bionic vascularized liver tissue and its application in hepatitis drug screening

[0046] 1) Preparation of cell-containing hydrogel: extract rat adipose stem cells (ADSC) and embryonic stem cells (ESC), and prepare cell suspension after culture and passaging; mix natural polymer powder with DMEM culture medium to obtain mass volume concentration 20% gelatin solution, 4% mass volume concentration of sodium alginate solution and 2% mass volume concentration of fibrinogen solution, the volume ratio of the above gelatin solution, sodium alginate solution and fibrinogen solution is 1:2 :1 After mixing, the natural polymer solution was obtained for use; the ADSC and ESC suspensions were centrifuged respectively, and mixed with the natural polymer solution respectively to obtain a cell concentration of 3×10 6 Individual / mL natural polymer solution containing ADSC, and the cell concentration is 1×10 5 A / mL natural polymer solution cont...

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Abstract

The invention discloses a personalized bionic composite structure as well as a preparation method of the composite structure and a method for drug screening by using the composite structure, and relates to a bionic composite structure based on a composite multi-nozzle three-dimensional printing technology and a preparation method thereof. The bionic composite structure comprises a polymer shell, a branched vascular stent, a functional tissue area, a vascular tissue area and polymer isolating layers, wherein the functional tissue area is a hydrogel layer containing tissue cells; the vascular tissue area is a hydrogel layer containing vascular seed cells; the functional tissue area and the vascular tissue area are alternately arranged in space; the polymer isolating layers are alternately distributed in the functional tissue area and the vascular tissue area, and divide the functional tissue area and the vascular tissue area into a plurality of small regions; the branched vascular stent includes two parts, i.e., artery blood vessel and vein blood vessel. According to the invention, the tissue / organ bionic structure with the branched blood vessel is prepared through the composite multi-nozzle 3D printing technology; the hydrogel structures are not cross-linked or polymerized; in vivo vascularized tissue and blood vessel states are simulated to the greatest extent, thus providing a reference for vascularized organ and tissue.

Description

technical field [0001] The invention belongs to the field of bioengineering, specifically to the field of biology or enzymes and their compositions, and relates to a personalized biomimetic composite structure and a method for its preparation and drug screening. Background technique [0002] In recent years, with the increasingly close communication between disciplines, cell arrangement technology has emerged, laying the engineering foundation for tissue engineering, organ manufacturing and drug screening. Among them, the prominent 3D printing technology, also called Rapid Prototyping (RP) technology, uses computer-aided modeling and discrete-stacking principles to process materials [Fedorovich NE, et al. Trends in biotechnology, 2011; 29(12): 601-606]. This technology can precisely position biomaterials and is highly valued in the field of in vitro assembly of complex organs. Tissues or organs formed in vitro are widely used, and can be used to study the basis of maintain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/02
Inventor 王小红许雨帆
Owner TSINGHUA UNIV
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