193 results about "Pullulan" patented technology

The invention belongs to the technical field of biological materials, and particularly relates to nano composite hydrogel as well as a preparation method and application thereof. In the preparation process of the hydrogel, preparation of gelatin hydrazide and preparation of pullulan oxidation are sequentially completed, then preparation of active component loaded PLGA nanoparticles and self-assembly coating with metal-polyphenol are completed, and finally, the metal-polyphenol coated PLGA nanoparticles are mixed with gelatin hydrazide and pullulan oxidation to form gel in situ. The hydrogel has the characteristics of injectability, self-healing, wet adhesion and the like, the metal-polyphenol layer has the effects of resisting inflammation, resisting oxidation and promoting angiogenesis and generates photo-thermal response to near infrared rays, on-demand controllable release of the drug-loaded nanoparticles is achieved, the composite hydrogel can improve the wound surface microenvironment, promote angiogenesis and inhibit inflammation and scar formation, and the drug-loaded nanoparticles can be applied to wound healing. The ointment is suitable for burns, scalds and various complex wounds.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention relates to the technical field of biological medicines, in particular to a soluble skin-adhering microneedle for delivering an inactivated enterovirus vaccine as well as a preparation method and application of the soluble skin-adhering microneedle. Sodium hyaluronate is adopted as a film forming material to wrap an enterovirus 71 type (EV71)-coxsackie virus A16 (CA16) type bivalent inactivated vaccine antigen, and a needle body layer of the soluble microneedle is formed; the substrate layer is prepared from a pullulan material, so that the microneedle patch with a stable overall structure is formed. The technology provided by the invention is completed in a clean environment, and the vaccine patch which is qualified through sterility detection is obtained. The soluble microneedle disclosed by the invention has good antigen stability under the condition of 2-8 DEG C. In animal experiments, the microneedle is attached to the skin surface of a mouse for skin-adhering immunization, a strong immune response reaction can be induced, good immunogenicity is shown, and a novel, safe and effective vaccine delivery way is provided for prevention and control of the infantile hand-foot-and-mouth disease.

The invention provides a multi-stage synergistic wound repair nanofiber membrane and a preparation method thereof. The composite fiber membrane sequentially comprises an electrostatic spinning pullulan / tannic acid / tranexamic acid nanofiber membrane for promoting the formation of blood clots, a polydopamine / calcium chloride modified electrostatic spinning cellulose acetate membrane with coagulating, antibacterial and repairing effects, and a chitosan composite cellulose non-woven fabric, and the electrostatic spinning pullulan / tannic acid / tranexamic acid nanofiber membrane is a wound surface contact layer. The nanofiber membrane has the functions of a physical hemostasis barrier and continuously releasing regeneration promoting biological signals, and a dynamic hemostasis healing process is formed.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The invention relates to the technical field of bioengineering, in particular to a preparation process for extracting antihypertensive peptide from euphausia superba. The process comprises the following steps: adding a ferulic acid / arginine double-grafted pullulan compound in a preparation process, and breaking an emulsification interface by utilizing beta-cyclodextrin adsorption and phospholipase A2 cracking to obtain a soluble pretreatment product; meanwhile, short-time round-trip pH pulse is implemented in the incision enzyme hydrolysis process to activate potential enzyme cutting sites, finally, hydrophobic aggregation peptides are removed through weak acid rapid elution, and excision hydrolysis is performed to obtain a target product with the molecular weight of 0.5-3 kDa, so that dynamic induction and antioxidant steady-state dual control of hydrolysis reaction is realized; the generation proportion of short peptides and the ACE inhibition persistence are improved, and the flavor and the storage stability are remarkably improved. The method is controllable in technological process, high in repeatability and suitable for large-scale preparation of the euphausia superba protein functional peptide and development and application of antihypertensive food.

The formation of scars at a wound site is reduced by contacting the wound site with an effective dose of an inhibitor of focal adhesion kinase (FAK) formulated in a pullulan hydrogel. The release profile of the FAK inhibitor can be adjusted according to the nature of the wound, e.g., excisional wounds, burn wounds, etc.

Disclosed herein are home care compositions and films formed therefrom. According to one aspect of the present invention, there is provided a home care composition comprising: a polysaccharide comprising Pulullan, an alginate, or a combination thereof; optionally, cellulose; a polyol; one or more surfactants, the one or more surfactants including an anionic surfactant, a betaine surfactant, a nonionic surfactant, or a combination of two or more thereof; optionally, a fragrance; and optionally an emollient, wherein the home care composition is in the form of a film.

The invention discloses a fresh-keeping storage method of plum fruits, which is characterized in that a compact network structure of a shellac-pullulan-gellan gum basic composition effectively obstructs invasion of external microorganisms, and significantly slows down loss of water in the fruits, so that the plump appearance and hardness of the fruits are maintained, and the fruit quality is improved. The siRNA and the AVG wrapped by the lipidosome accurately interfere with the ripening and senescence process in the fruit from the molecular level, the siRNA specifically silences the gene expression of cell wall degradation key enzyme and delays the softening process of the fruit, and the AVG effectively blocks the biosynthesis pathway of ethylene and inhibits the start of an after-ripening signal, so that the ripening and senescence process of the fruit is delayed. The external formed physical film provides a stable microenvironment for internal bioactive molecules, protects the internal bioactive molecules from being degraded and prolongs the action time, and the internal molecular regulation delays the disintegration of cell structures, so that the external film can be attached more enduringly and exerts a barrier function, and the maintenance of the fruit quality is realized under a refrigeration condition.

The invention discloses a high-wettability and strong-adhesion cross-linking enhanced dust fall material. The dust fall material comprises the following raw materials in parts by mass: 2-5 parts of pullulan, 2-5 parts of agarose, 5-15 parts of sodium hydroxide, 5-15 parts of chloroacetic acid, 5-15 parts of ethylene glycol diglycidyl ether, 3-11 parts of malic acid, 5-10 parts of a water-retaining agent, 5-10 parts of diluted hydrochloric acid and 1000-2500 parts of distilled water. The dust falling material has high wettability and strong adhesion, and can effectively settle coal dust in air; meanwhile, a coal dust consolidation layer formed after the dust falling material is sprayed is high in strength, reentrainment of dust can be effectively prevented, and the technical problems that the dust falling material is poor in dust falling effect, single in function and the like are solved.

The invention relates to aureobasidium pullulans Cy28 and application thereof, and belongs to the technical field of microorganisms, the aureobasidium pullulans Cy28 can reduce the content of Cd in soil and can generate more pullulan in a Cd-containing environment; when the culture medium is applied to lettuce planting, the dry weight of edible tissues of the lettuce can be remarkably increased in a cadmium-free or cadmium-containing environment, and the content of Cd in the edible tissues and roots of the lettuce is reduced, so that the content of Cd in the edible tissues of the lettuce reaches the edible standard; in addition, the vitamin C content, the chlorophyll content, the soluble protein content and the IAA content of edible tissues of the lettuce can be improved; meanwhile, the content of beneficial microorganism Pseudarthrobacter in the soil can be remarkably increased in a cadmium environment, and the effect of improving the soil is achieved.

A composition for application to a surface of skin includes a carrier, a plasticizer, a non-water solvent, salicylic acid, a gum and / or a polymer binder-film former, an occlusive agent, an emollient, and a pullulan. The composition may form a thin and durable protective film on the surface of skin after application. Methods of making and using the composition are further disclosed.

The invention discloses a preparation method of a whey protein / pullulan / sugar astaxanthin nano-dispersion, WPC / PUL compounds are adopted to construct different carriers to embed AST, the carriers are respectively a nano-dispersion loaded with AST, emulsion gel and microcapsule powder, the three carriers have good embedding efficiency and protection effect on AST, the simulated gastrointestinal digestion stability of AST is remarkably improved, and the stability of AST is improved. And the method has great significance in expanding the preparation of an AST embedding delivery system and the application of AST in industries such as food and the like.

The present invention relates to a film and a capsule which do not contain titanium oxide and which have light-blocking properties and improved mechanical strength. The film and capsule are produced using a composition comprising a film-forming polymer and a whitening agent. The film-forming polymer is selected from the group consisting of cellulose derivatives, gelatin, and pullulan. The whitening agent comprises a salt and a surfactant, wherein the salt is sodium sulfate or a combination of sodium sulfate and a white pigment, wherein the white pigment is not titanium dioxide. The light-blocking film and capsule having improved mechanical strength are obtained by molding the composition into a film and a capsule, respectively, and then drying the film and capsule at 55°C or lower.

The invention provides a pulullan refining method, which belongs to the technical field of biology, and specifically comprises the following steps: fermenting a microbial strain for producing pulullan to obtain fermentation liquor, and sterilizing and decoloring the fermentation liquor to obtain a crude pure extracting solution; carrying out a membrane process and a resin process on the obtained crude pure extracting solution to obtain a refined pure solution; the pore diameter of the membrane material is 500-1000Da; according to the method disclosed by the invention, it is found for the first time that the pullulan is extracted and purified by using a small-aperture nanofiltration membrane and is matched with resin adsorption, so that the purification effect can be achieved, the purity of the product is improved, and the yield of the product is also obviously improved.

This invention discloses a pullulanase mutant with enhanced catalytic activity against highly branched dextrin substrates, its construction method, and its applications, belonging to the fields of enzyme engineering and biotechnology. This invention obtains two mutants, D139A and D139E, by site-directed mutagenesis of the pullulanase derived from Klebsiella pneumoniae at position 139 aspartic acid residue. Compared with the wild-type enzyme, both mutants maintain high hydrolytic activity against pullulan polysaccharides while significantly improving hydrolytic activity against dextrin substrates with high α-1,6 glycosidic bond content. Specifically, the hydrolytic activity against dextrin substrates with 6% and 10% α-1,6 bond content is increased by up to 242% and 110%, respectively. The pullulanase mutants obtained in this invention exhibit excellent catalytic performance in the enzymatic debranching reaction of highly branched dextrin substrates, showing promising application prospects in starch deep processing and related biomanufacturing fields.

The invention discloses a hangover-alleviating and liver-protecting preparation, which belongs to the technical field of pharmacy and comprises a porous skeleton, a targeted nano core dispersed in pores of the porous skeleton and a conditioning layer, the porous framework is formed by taking sodium alginate, chitosan and pullulan as base materials, and an effervescing agent is uniformly dispersed in the porous framework and comprises organic acid and bicarbonate; the targeted nano core comprises an ionic cross-linked dual network formed by film-forming polysaccharide and gellan and is coated with an enteric layer; alcohol effect dispelling layer powder is further dispersed in the porous framework and comprises one or more of kudzuvine roots, hovenia dulcis thunb, turmeric extracts, taurine, emblic leafflower fruit extracts, corn oligopeptide powder, rice bran fatty alkanol, ammonium glycyrrhizinate and mung beans. The conditioning layer comprises one or two of resistant dextrin and soluble dietary fiber. The hangover-alleviating and liver-protecting preparation provided by the invention simultaneously realizes near-end intervention of controllable retention and quick release in the stomach, efficient and in-place liver-protecting release of the small intestine section and far-end support of continuous conditioning of the colon section.

The invention relates to a bagging-free quality-improving and efficiency-improving pesticide for reducing the insect fruit rate of pomes and a spraying method of the bagging-free quality-improving and efficiency-improving pesticide. The quality-improving and efficiency-improving pesticide comprises the following components: 1-100 g / L of pullulan; 0.5 g / L to 2 g / L of EDTA-2NaCa; the nutrient solution is prepared from 0.3 g / L to 5 g / L of L-calcium aspartate, 1 g / L to 3 g / L of D-calcium gluconate, 2 g / L to 4 g / L of sucrose fatty acid ester, 5 g / L to 15 g / L of tea polyphenol, 2 g / L to 6 g / L of gallic acid, 0.5 mg / L to 1.5 mg / L of brassinolide, 2 g / L to 4 g / L of fumed silica, 0.1 g / L to 0.3 g / L of xanthan gum, 1 g / L to 3 g / L of D-sorbitol, 0.1 g / L to 0.5 g / L of an anti-coagulant and water serving as a solvent. After the pesticide is sprayed, the pest prevention and control effect is equal to that of bagging, the cost is reduced by 68%, sunburn and bitter pox diseases are reduced, the chlorophyll SPAD value is increased by 22%, the photosynthetic rate is increased by 18%, and high yield is promoted.

This invention provides a pullulan polysaccharide hemostatic sponge and its preparation method. The sponge comprises a double-network structure consisting of a skeletal layer and an interpenetrating layer. The skeletal layer is formed by aldol condensation of pullulan polysaccharide, constituting the outer network; the interpenetrating layer is formed by ionic crosslinking of sodium alginate and gallium ions, constituting the inner network; the skeletal layer and the interpenetrating layer are connected by borate ester bonds, thus forming the pullulan polysaccharide hemostatic sponge. The sponge of this invention not only has high porosity and water absorption rate, enabling it to quickly absorb excess blood from the wound surface for rapid and effective hemostasis and promoting wound coagulation, but also possesses antibacterial and hemostatic properties and shape memory function, making it applicable to various environments.

The present invention relates to a gel composition and a method of its preparation and use, wherein the composition comprises 1-30 wt% pectin, 1-20 wt% tara gum, and 6-70 wt% pullulan, the composition substantially not comprising carrageenan and starch.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

The invention provides a mildewproof and antibacterial edible packaging film as well as a preparation method and application thereof. The mildewproof and antibacterial edible packaging film comprises soybean protein isolate, carrageenan, pullulan, modified cellulose, a plasticizer, a reinforcing agent and a preservative. According to the application, a six-element synergistic system of modified cellulose, soybean protein isolate, pullulan, carrageenan, lysine and calcium propionate is formed; the modified cellulose is used as a film forming main body, so that the water sensitivity is low and is not easily influenced by environmental temperature and humidity; the soybean protein isolate and the pullulan synergistically provide high barrier property; carrageenan improves the mechanical property and compactness of the film in a blending system; free calcium of calcium propionate provides a bridge effect for crosslinking of protein and carrageenan, and the mechanical property and compact structure of the film are improved; lysine and a blending system can form a'charge-hydrogen bond-hydrophobicity 'multi-synergistic network, so that the mechanical and barrier properties of the film are improved, and meanwhile, the mildew resistance of calcium propionate is coordinated. The film can meet the packaging of superfine powder materials, and is applied to the extreme temperature and humidity environment with the temperature ranging from-20 DEG C to 50 DEG C and the RH ranging from 30% to 95%.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The invention relates to the technical field of wine, in particular to rose and litchi flavored compound wine and a preparation process thereof. The preparation method comprises the following steps: mixing a rose extract with a grease carrier, mixing with an aqueous solution of whey protein isolate and sodium caseinate, homogenizing, adding sodium alginate, adjusting the pH value, further reacting by using transglutaminase and calcium chloride, and then adding pullulan to prepare the rose extract compound. The obtained compound is excellent in ethanol solution resistance and high in stability, can stably exist in low-alcohol compound wine, and can further shorten the failure time of active ingredients of the rose extract and improve the taste and fragrance of the compound wine in the storage process. In addition, the rose extract compound, the litchi concentrated juice and other active ingredients are adopted for preparing the low-alcohol compound wine, and the low-alcohol compound wine is lasting in fragrance, moderate in taste and good in application effect.

Disclosed herein is a water-soluble film comprising pullulan, carrageenan, a plasticizer, and a crosslinking agent, wherein the pullulan comprises from about 50 wt.% to about 75 wt.% of the film, and the crosslinking agent, when present, comprises from about 0.001 wt.% to about 5.0 wt.% of the film, based on the total weight of the film.

Water-soluble films comprising: a film-forming resin; and optionally, a plasticizer; wherein: the film-forming resin comprises a mixture of a water-soluble polyvinyl alcohol (PVOH) and a biopolymer (or natural polymer); the PVOH is present in an amount up to about 40 wt.%, based on the total weight of the film-forming resin; and the biopolymer is present in an amount of at least about 60 wt.%, based on the total weight of the film-forming resin; with the provisos that: (a) when the biopolymer comprises pullulan, pullulan is not the sole biopolymer in the film; and (b) when the biopolymer comprises starch, starch is not the sole biopolymer in the film. Multiple combinations of biopolymers are contemplated.

Film products, especially suitable for oral delivery, which can be formed during manufacture in the form of large and / or heavy film strips or sheets and subsequently cut into uniform dosage units, each dosage unit being uniform in content and having distributed therein a linear polysaccharide, such as pullulan, a plasticizer, and an active component.

The present application provides a kind of meal replacement gel, raw materials include konjac glucomannan, pullulan, sodium carboxymethyl cellulose, citric acid and tea polyphenol;Wherein, the meal replacement gel is formed by chemical crosslinking of konjac glucomannan, pullulan, sodium carboxymethyl cellulose under the action of citric acid, and by physical crosslinking of konjac glucomannan, pullulan and sodium carboxymethyl cellulose under the action of tea polyphenol;By chemical crosslinking and physical crosslinking constitute interpenetrating network, so that meal replacement gel obtains high swelling performance, simultaneously by adjusting the weight part of citric acid and tea polyphenol adjusts the proportion of chemical crosslinking and physical crosslinking in interpenetrating network, to regulate the swelling performance of meal replacement gel.

The invention provides low-irritation nicotine gel as well as a preparation method and application thereof. The nicotine gel comprises a gel matrix and an exosome compound embedded in the gel matrix, the gel matrix is prepared from the following components: pullulan and water; the exosome compound comprises a pomegranate exosome and nicotine loaded in the pomegranate exosome. According to the pomegranate exosome, the nicotine is coated in the pomegranate exosome, so that the stability of the nicotine can be effectively improved, the skin permeability of the nicotine is enhanced, and the abstinence symptom occurring during smoking cessation is relieved; pulullan is used as a medium, the skin absorption effect of nicotine is further regulated and controlled, and the situation that the smoking cessation effect is affected by too fast or too slow absorption is avoided.