9results about How to "Improve polysaccharide yield" patented technology

The invention provides velvet antler mushroom sporocarp water extraction residue polysaccharide as well as an extraction method and application thereof, and belongs to the technical field of edible mushroom polysaccharide preparation. The invention provides an extraction method of water extraction residue polysaccharide of a mushroom fruiting body, which comprises the following steps: sequentially carrying out solid state fermentation, post-treatment and hot water extraction on the water extraction residue of the mushroom fruiting body to obtain an extraction product containing the water extraction residue polysaccharide of the mushroom fruiting body. According to the method disclosed by the invention, the water extraction residues of the pilose antler mushroom sporocarp are used as raw materials, so that high-value utilization of the water extraction residues of the pilose antler mushroom sporocarp can be realized. Moreover, the method can improve the yield and content of polysaccharide, and has the advantages of no chemical residue, ecological environmental protection, high production efficiency and the like. Besides, the polysaccharide prepared by the extraction method disclosed by the invention is stable under a digestion condition, and the prepared polysaccharide can improve the content of short-chain fatty acids in intestinal tracts, so that a basis is provided for application of the polysaccharide of the water-extracted residues of the fruiting bodies of the velvet antler mushrooms in new potential prebiotics.

The invention belongs to the technical field of extraction processes, and particularly relates to a process for extracting auricularia auricula polysaccharide by a compound enzyme-assisted subcritical water extraction method. The method specifically comprises the following steps: performing degreasing treatment on agaric to obtain degreased agaric powder; mixing the degreased auricularia auricula powder with water, then adding cellulose-pectin compound enzyme into the mixture, and performing enzymolysis to obtain an enzymolysis auricularia auricula solution; and carrying out subcritical extraction on the enzymolysis agaric solution to obtain the agaric polysaccharide. The compound enzyme-assisted subcritical water extraction method disclosed by the invention not only effectively shortens the extraction time, but also has the advantages of improving the polysaccharide yield and the polysaccharide biological activity.

The invention discloses a preparation method of Chuzhou chrysanthemum water-soluble polysaccharide and application of the Chuzhou chrysanthemum water-soluble polysaccharide as a rice juice stabilizer, and belongs to the technical field of Chuzhou chrysanthemum polysaccharide extraction.The preparation method comprises the steps that firstly, Chuzhou chrysanthemum is soaked and pretreated with ethyl alcohol, then the ethyl alcohol is removed, and pretreated chrysanthemum filter residues are obtained; 2, the obtained pretreated chrysanthemum filter residues are subjected to water extraction, a sodium bicarbonate water solution serves as a water extraction system, and first-step treatment is conducted for 5-10 min under the dual-action conditions of 10-15 KHz ultrasonic treatment and 200-300 Mpa pressure; then carrying out intermittent 10-15 KHz ultrasonic treatment, and carrying out second-step treatment for at least 1 hour under the conditions that the air pressure is 50-60 kpa and the temperature is 80-86 DEG C, so as to obtain a water extraction filtrate; 3, mixing the obtained water extraction filtrate with ethanol, and precipitating, wherein the ethanol concentration in the mixed system is 35-50%; and 4, collecting the precipitate, dissolving the precipitate, dialyzing with running water, and freeze-drying to obtain the water-soluble polysaccharide of Chuzhou chrysanthemum. According to the method, the polysaccharide obtaining rate is increased.

This invention belongs to the technical field of pharmaceuticals, health foods, and medicines, specifically relating to Russula polysaccharide, its extraction method, and its application in anti-inflammatory, antioxidant, and / or hypoglycemic effects. This invention employs an ultrasound-assisted enzymatic hydrolysis method to extract Russula polysaccharide, thereby significantly improving extraction efficiency, reducing enzyme dosage, protecting the polysaccharide structure, and increasing yield and purity. Furthermore, experimental verification shows that it possesses good anti-inflammatory, antioxidant, and / or hypoglycemic effects, and when combined with Phyllanthus emblica polysaccharide, it synergistically enhances hypoglycemic performance, thus demonstrating significant practical application value.

The invention belongs to the technical field of polysaccharide extraction, and particularly relates to a boschniakia rossica polysaccharide extraction method. The boschniakia rossica polysaccharide extract is obtained by dispersing boschniakia rossica powder in an ethanol solution, then adding a compound enzyme, adjusting the pH value to 5-7, and carrying out ultrasonic treatment, enzyme deactivation and centrifugation. Compared with a traditional single extraction method, the extraction method has the advantages that the extraction process of the boschniakia rossica polysaccharide is optimized through the response surface method, and compared with the traditional single extraction method, the extraction method is practical and reliable, can be used for extracting the boschniakia rossica polysaccharide, and can remarkably improve the polysaccharide yield which can reach 13.67%.

The invention discloses a heartleaf houttuynia herb beverage based on ultrahigh pressure synergetic enzymolysis, which belongs to the technical field of beverages and is prepared from the following components: a distillation extract of fresh heartleaf houttuynia herb roots, a leaching solution of the fresh heartleaf houttuynia herb roots, an ultrahigh pressure synergetic enzyme extract of heartleaf houttuynia herb residues and auxiliary materials, the ultrahigh-pressure synergistic enzyme extract of the herba houttuyniae residues is obtained by drying and crushing residues obtained by distilling and extracting fresh roots of herba houttuyniae, mixing the obtained powder with water to form slurry, performing ultrahigh-pressure pretreatment on the slurry, performing enzymolysis treatment on the slurry subjected to ultrahigh-pressure pretreatment, and performing solid-liquid separation and concentration. According to the method disclosed by the invention, after the ultrahigh pressure pretreatment, the efficiency of the subsequent enzymolysis reaction is greatly improved, and the enzyme can directly act on the substrate, so that the dosage of the enzyme is reduced, the enzymolysis time is shortened, and the comprehensive cost is reduced while the polysaccharide yield is improved; plant cell walls can be instantly, uniformly and physically broken through ultrahigh pressure (300-600 MPa) treatment, so that components such as intracellular polysaccharides are fully exposed, and the problem of component dissolution diffusion in a traditional pure water extraction or enzymolysis method is solved.

The invention relates to the technical field of food biology. In particular to a preparation method of mesona jelly and application of the mesona jelly in preparation of drinks. By optimizing the enzyme composition, when the mesona chinensis jelly is prepared, the extraction efficiency of mesona chinensis polysaccharide is obviously improved, and the extracted mesona chinensis polysaccharide has very good oxidation resistance. The mesona chinensis jelly with high mesona chinensis polysaccharide content has better health efficacy when being used for preparing food. The method is low in energy consumption, simple to operate and suitable for large-scale production and preparation in food factories.

This invention discloses a method for extracting polysaccharides from the stipe of *Morchella esculenta* and its application, relating to the field of polysaccharide extraction technology. The *Morchella esculenta* stipe polysaccharide is D-MSPS-1, mainly composed of mannose, glucose, and galactose in a molar ratio of 25.88:65.17:8.95, with a weight-average molecular weight of 64202. The method includes the following steps: Step 1: Drying, pulverizing, and sieving the *Morchella esculenta* stipe; Step 2: Ultrasonic extraction using a eutectic solvent, collecting the supernatant, adding anhydrous ethanol, precipitating overnight, centrifuging to collect the precipitate, obtaining crude *Morchella esculenta* stipe polysaccharide; Step 3: Removing proteins from the crude polysaccharide in Step 2 using a potassium ferrocyanide-zinc acetate method, and adsorbing pigments using macroporous resin to obtain crude polysaccharide D-MSP; Step 4: Separating and purifying D-MSP using a chromatography column to obtain homogeneous D-MSPS-1. This invention provides a technical reference for the green and efficient extraction of fungal stem polysaccharides, and provides preliminary experimental basis for their potential application in the development of functional foods and tumor adjuvant therapy products.

The invention discloses an extraction method and application of Vanis lindera and its spore powder polysaccharide. The Vanis lindera spore powder polysaccharide is used for selective separation of polysaccharide in Vanis lindera spore powder sample liquid treated by cellulose enzymolysis, ultrasound, and enzymolysis-assisted ultrasound pretreatment through a separation system composed of tert-butyl alcohol and inorganic salt ammonium sulfate. This is the first time to separate active polysaccharide from lindera spore powder. The Vanis lindera spore powder polysaccharide of the invention has high-efficiency antioxidant, hypoglycemic, immunostimulatory and anti-tumor capabilities, and can be widely used in functional foods, cosmetics, medicines, etc.