3results about How to "Improve polysaccharide yield" patented technology

This invention belongs to the technical field of pharmaceuticals, health foods, and medicines, specifically relating to Russula polysaccharide, its extraction method, and its application in anti-inflammatory, antioxidant, and / or hypoglycemic effects. This invention employs an ultrasound-assisted enzymatic hydrolysis method to extract Russula polysaccharide, thereby significantly improving extraction efficiency, reducing enzyme dosage, protecting the polysaccharide structure, and increasing yield and purity. Furthermore, experimental verification shows that it possesses good anti-inflammatory, antioxidant, and / or hypoglycemic effects, and when combined with Phyllanthus emblica polysaccharide, it synergistically enhances hypoglycemic performance, thus demonstrating significant practical application value.

The invention discloses a heartleaf houttuynia herb beverage based on ultrahigh pressure synergetic enzymolysis, which belongs to the technical field of beverages and is prepared from the following components: a distillation extract of fresh heartleaf houttuynia herb roots, a leaching solution of the fresh heartleaf houttuynia herb roots, an ultrahigh pressure synergetic enzyme extract of heartleaf houttuynia herb residues and auxiliary materials, the ultrahigh-pressure synergistic enzyme extract of the herba houttuyniae residues is obtained by drying and crushing residues obtained by distilling and extracting fresh roots of herba houttuyniae, mixing the obtained powder with water to form slurry, performing ultrahigh-pressure pretreatment on the slurry, performing enzymolysis treatment on the slurry subjected to ultrahigh-pressure pretreatment, and performing solid-liquid separation and concentration. According to the method disclosed by the invention, after the ultrahigh pressure pretreatment, the efficiency of the subsequent enzymolysis reaction is greatly improved, and the enzyme can directly act on the substrate, so that the dosage of the enzyme is reduced, the enzymolysis time is shortened, and the comprehensive cost is reduced while the polysaccharide yield is improved; plant cell walls can be instantly, uniformly and physically broken through ultrahigh pressure (300-600 MPa) treatment, so that components such as intracellular polysaccharides are fully exposed, and the problem of component dissolution diffusion in a traditional pure water extraction or enzymolysis method is solved.

This invention discloses a method for extracting polysaccharides from the stipe of *Morchella esculenta* and its application, relating to the field of polysaccharide extraction technology. The *Morchella esculenta* stipe polysaccharide is D-MSPS-1, mainly composed of mannose, glucose, and galactose in a molar ratio of 25.88:65.17:8.95, with a weight-average molecular weight of 64202. The method includes the following steps: Step 1: Drying, pulverizing, and sieving the *Morchella esculenta* stipe; Step 2: Ultrasonic extraction using a eutectic solvent, collecting the supernatant, adding anhydrous ethanol, precipitating overnight, centrifuging to collect the precipitate, obtaining crude *Morchella esculenta* stipe polysaccharide; Step 3: Removing proteins from the crude polysaccharide in Step 2 using a potassium ferrocyanide-zinc acetate method, and adsorbing pigments using macroporous resin to obtain crude polysaccharide D-MSP; Step 4: Separating and purifying D-MSP using a chromatography column to obtain homogeneous D-MSPS-1. This invention provides a technical reference for the green and efficient extraction of fungal stem polysaccharides, and provides preliminary experimental basis for their potential application in the development of functional foods and tumor adjuvant therapy products.