75results about How to "Improve polysaccharide yield" patented technology

The invention discloses an extraction and purification method of bioactive polysaccharides in dendrobium huoshanense. The method comprises the following steps: crushing the dendrobium huoshanense, then adding water, performing microwave constant-temperature extraction, and performing vacuum concentration on extract solution till the relative density is 110-1.30 so as to get concentrated solution;adding ethanol into the concentrated solution, standing, and further performing centrifugation to get precipitate; adding the water into the precipitate for dissolution, enabling the solution to passthrough an ultrafiltration membrane in the molecular weight of 100KD under the pressure of 0.35MPa, collecting trapped fluid, then enabling the trapped fluid to pass through the ultrafiltration membrane in the molecular weight of 300KD under the pressure of 0.35MPa, and collecting passing fluid; and performing the vacuum concentration on the collected passing fluid, and drying to get a polysaccharide extract from the dendrobium huoshanense. By adopting the method, the extraction time can be shorted, and the yield of a crude extract can be improved from 20% to about 35% under the same extraction times; and the yield of the polysaccharides from the dendrobium huoshanense after purification can be improved from about 5% to 10-15%, and the content of the polysaccharides from the dendrobium huoshanense can be improved from 40% to above 60%.

The invention discloses a process for extracting polysaccharide from a dendrobium officinale leaf according to an enzymatic degradation method. According to the process, a pure product of the dendrobium officinale polysaccharide is obtained by drying the dendrobium officinale leaf, crushing, backflowing with an organic solvent, collecting a dreg of a decoction, drying the dreg of the decoction, adding distilled water to dissolve, conducting ultrasonic treatment, conducting enzymolysis by using cellulase, pectinase and papain, heating to denature the enzyme, centrifuging, filtering, decoloringa filtrate by using an activated carbon, filtering and collecting the filtrate again, concentrating in vacuum, adding an organic solvent to conduct alcoholysis, filtering and collecting a precipitateagain, airing to obtain a crude product of the dendrobium officinale polysaccharide, adding distilled water to dissolve, dialyzing with a nanofilm, adding the organic solvent to conduct alcoholysis, filtering and collecting a precipitate again, and finally freeze-drying. The yield of the prepared dendrobium officinale polysaccharide is high, the operation condition of the enzymatic method is relatively mild, and the process has the advantages of high efficiency, energy saving, low pollution and the like.

The invention discloses a ganoderma lucidum polysaccharide extracting method. The extracting method comprises the steps: firstly, smashing ganoderma lucidum sporocarp and sieving by a 80-mesh sieve; then putting into a steam explosion device to be exploded, adding into water to be soaked for 1.5h according to a solid to liquid ratio as 1 to (10 to 20) and then heating to 30 to 50 DEG C to performultrasonic treatment; adjusting a material liquid temperature, a pH value, protease and cellulase; after enzymolysis finishes, warming to denaturalize enzyme, centrifuging, decoloring resin, deproteinizing, filtering, vacuum concentrating and vacuum drying under a lower temperature to obtain high-activity and high-purity ganoderma lucidum polysaccharide solid powder. Therefore, a polysaccharide extracting rate and a polysaccharide production efficiency are improved, a space structure of polysaccharide is prevented from being damaged, biological activity of the polysaccharide is prevented frombeing reduced, and the ganoderma lucidum polysaccharide extracting method is more suitable for industrial production.

The invention discloses a tremella polysaccharide extracting method, and belongs to the technical field of edible fungus deep processing. The method comprises the steps of smashing, enzyme-extraction, alkaline extraction, primary concentration, protein removing, secondary concentration and drying, wherein an enzyme used in the step of enzyme-extraction is a compound enzyme which is obtained by mixing protease, cellulase and pectinase according to the mass ratio of 0.5 to 1 to 2-4. According to the tremella polysaccharide extracting method, by means of the matching design of the compound enzyme and adding the step of alkaline extraction, the yield of polysaccharide is greatly increased, and therefore the economic benefit of an edible fungus deep processing industry is improved.

The invention relates to a method for preparing a pure polysaccharose from alfalfa hay and provides the method for preparing the pure alfalfa polysaccharose, which can be subject to mass production and is simple in process, low in cost and high in efficiency. The method comprises the following steps of: crushing aired or dried alfalfa; soaking the powder in a certain amount of water, wherein the ratio of the powder to the water is 1:20-1:45; heating the mixture at the temperature of 55 to 85 DEG C for 10 to 35 minutes under the action of 40 to 60kHz ultrasonic wave of which the power is 240 to 400W; filtering the mixture and collecting the filtrate; concentrating the obtained filtrate until the volume of the filtrate is reduced to one third; removing proteins from the filtrate with n-butyl alcohol-trichloromethane solution, wherein the volume ratio of n-butyl alcohol to hloromethane is 1 to 4; performing alcohol precipitation on the filtrate with ethanol of which the volume is 2 to 4.5 times that of the filtrate; after standing the filtrate, centrifugating the filtrate at the speed of 3,000 to 5,000 rpm for 10 to 20 minutes; freezing and drying the lower layer of precipitate at the temperature of -70 to -50 DEG C to obtain the e polysaccharose; and separating and purifying the polysaccharose through two kinds of cellulose column chromatography to obtain the high-purity polysaccharose. When the method is compared with the traditional method, the cost is reduced; the production efficiency is greatly improved; and the purity of the obtained polysaccharose product can be up to over 96 percent.

The invention discloses a method for increasing polysaccharide content of morel submerged fermentation mycelium through astragalus extract solution. The method is characterized in that the astragalus extract solution is added to morel submerged fermentation culture solution to promote the growth of morel mycelium so as to increase the morel polysaccharide content. According to the method, active ingredients (alkaloid, saponin, flavones and the like) of astragalus can promote or inhibit the growth of medical fungal mycelium and the growth of metabolite.

The invention discloses a purification method of low-molecular-weight sargassum fusiforme polysaccharides. The purification method comprises the following steps of (1): ultrafiltration classification:enabling an argassum fusiforme polysaccharide solution containing different molecular weights to pass through ultrafiltration systems, the molecular weight cutoffs of which are 10kDa, 5kDa and 1kDa respectively and which are further sequentially connected in series, and enabling primary tail liquid of the 10kDa ultrafiltration system to flow into a circulation cup of the 5kDa ultrafiltration system to carry out water circulation ultrafiltration, wherein the weight-average molecular weight of the primary tail liquid is smaller than and equal to 10kDa; enabling secondary tail liquid of the 5kDaultrafiltration system to flow into a circulation cup of the 1kDa ultrafiltration system to carry out the water circulation ultrafiltration until tertiary tail liquid of the 1kDa ultrafiltration system does not contain polysaccharide, wherein the weight-average molecular weight of the secondary tail liquid is smaller than and equal to 5kDa; afterwards, rinsing the polysaccharide solution in pipelines of the ultrafiltration systems by using water and collecting respectively; (2), concentration and freeze-drying: collecting the polysaccharide solution of each interval section, carrying out reduced pressure concentration, and then carrying out reduced pressure freezing-drying, so that the low-molecular-weight sargassum fusiforme polysaccharides of intervals of the corresponding molecular weights can be obtained.

The invention discloses a method for extracting polysaccharide of pineapple pomace. The method for extracting the polysaccharide of the pineapple pomace comprises the following steps of pretreatment,degreasing, enzymolysis of a raw material, water extraction of filter residues, concentration, alcohol precipitation, deproteinization, depigmentation, drying and the like, wherein the extraction rateof the polysaccharide of the pineapple pomace can be improved effectively by adopting xylanase and pectinase Y-23 as a mixed enzyme preparation, then a trichloroacetic acid-butanol mixed solution isadded for deproteinization of crude polysaccharide, X-5 macroporous adsorption resin is added for the depigmentation of the deproteinized polysaccharide solution, and the purity of the polysaccharideof the pineapple pomace can be improved by controlling the conditions of the steps. The method for extracting the polysaccharide of the pineapple pomace is simple and rapid, and compared with a hot water extraction method, an ultrasonic wave-assisted extraction method and a cellulase extraction method, the obtained polysaccharide of the pineapple pomace has a high extraction rate, high purity andgood antioxidant activity, and can be used for preparing functional food, cosmetics and the like.

The invention discloses a preparation method of large-leaf yellow tea polysaccharide with a blood sugar reducing effect. The preparation method is characterized in that large-leaf yellow tea polysaccharide LYT-2 is prepared by taking large-leaf yellow tea produced from Huoshan as a raw material, performing solid-state fermentation through EM bacteria and carrying out extracting by utilizing a deepeutectic solvent technology; the molecular weight of the polysaccharide compound is 4.38x104 Da, the polysaccharide compound contains 4.98% of binding protein, and the molar ratio of mannose to glucose to arabinose is 2.16:4.83:1 in monosaccharide composition. In-vivo experimental data shows that LYT-2 can obviously reduce the weight and blood sugar level of high-sugar and high-fat mice. The result shows that LYT-2 has good application prospects in the aspects of food, medicine, health care and the like.

The invention discloses a complex enzymatic extraction method for laminarin and the obtained laminarin. The method comprises the following steps: taking kelp processing waste as a raw material, drying and crushing the kelp processing waste first, extracting and adding a proper amount of kelp powder into a fully-automatic hydrolysis reactor, adding a buffer solution with pH 6.0 for uniform stirring according to a solid-liquid ratio of 1:(35-45)ml, and adding complex enzymes, the additions of cellulase, hemicellulase, pectinase and protease being 0.004 to 0.006 grams, 0.006 to 0.008 grams, 0.004 to 0.006 grams and 0.001 to 0.014 grams based on the mass of 1 gram of the kelp powder; regulating the pH value to be 5.5 to 6.5, performing enzymatic hydrolysis for 3 to 4h at 45 to 55 DEG C, raising the temperature for enzyme deactivation, extracting hydrolysate, performing suction filtration to remove precipitates and insoluble substances, adding ethanol into supernatant until the volume fraction of the ethanol of the system is 65 to 75 percent, and performing cold storage overnight in a refrigerator of 4 DEG C; performing centrifugation for 20min at 3,000 to 4,000g/min, and performing freeze-drying on precipitates to obtain crude laminarin. According to the method and the laminarin, the yield and purity of the laminarin can be effectively improved under a mild condition, the structure and bioactivity of the laminarin can be completely preserved, and toxic substances and environmental pollution in a process are avoided.

The invention mainly relates to a preparation method of low molecular glossy ganoderma polysaccharide, in particular to the preparation method of low molecular ganoderma lucidum polysaccharide. The method comprises the steps of grinding, water extracting, concentrating, grading acetone sinking and centrifugal drying. The preparation method of the low molecular glossy ganoderma polysaccharide is simple, convenient, speedy and efficient, has low cost, few steps, no pollution, and high polysaccharide purity and is suitable for industrialization production.

The invention specifically relates to an extraction method for peach gum polysaccharide, belonging to the technical field of extraction of natural active components. The extraction method comprises the following steps: subjecting dried peach gum to impurity removal and crushing and carrying out treatment at 60 to 65 DEG C; then mixing the treated peach gum with distilled water under stirring for extraction; carrying out centrifugation with a high-speed centrifuge so as to obtain a supernatant; subjecting the supernatant to vacuum concentration; then adding liquid nitrogen into a concentrate obtained in the previous step; storing the concentrate at 2 to 3 DEG C and removing a precipitate; adding absolute ethyl alcohol into the concentrate without the precipitate; and subjecting another precipitate obtained in the previous step to vacuum drying and grinding so as to obtain the peach gum polysaccharide. Unlike conventional methods where proteins in peach gum are removed through a Sevage process, the extraction method provided by the invention realizes removal of proteins in the peach gum by adding liquid nitrogen into the concentrate according to a volume ratio of the concentrate to the liquid nitrogen of 10: 1-2, and the removal rate of proteins reaches 99.5%; moreover, the process of removal poses no pollution to the peach gum polysaccharide; and thus, the high-quality peach gum polysaccharide is finally obtained.

The invention discloses an extracting method for pleurotus eryngii polysaccharide, and belongs to the technical field of plant active ingredient extraction. The extracting method comprises the following steps: firstly, separating micromolecule components to the greatest extent by taking a low-concentration alcohol solution as a solvent, and supplementing with ultrasonic treatment; and then, extracting by taking water as a solvent and adopting an ultrasonic-inner boiling method and precipitating pleurotus eryngii polysaccharide from the high-concentration alcohol solution. Compared with conventional methods such as a water extraction method and an alcohol extraction method, the extracting speed and the extracting rate are obviously increased; the pleurotus eryngii polysaccharide yield is higher than or equal to 11% which is improved by 17-59% in comparison with that of the conventional method; and moreover, the activity of the pleurotus eryngii polysaccharide is kept to the greatest extent, so that the whole extracting process is short in consumed time, is high in yield, is less in impurities and is low in cost.

The invention discloses a preparation method of inonotus obliquus polysaccharides. The method comprises the following steps: firstly, carrying out superfine grinding on inonotus obliquus, then breaking walls, extracting with hot water and performing centrifugation to obtain an extracting solution, and carrying out the steps of microfiltration and ultrafiltration on the extracting solution, aqueoustwo-phase extraction, macroporous adsorption resin decolorization, nanofiltration desalting and concentration, freeze-drying and the like to obtain an inonotus obliquus polysaccharide product. The preparation method of the inonotus obliquus polysaccharides disclosed by the invention has the advantages that 1, the release of polysaccharide substances in the inonotus obliquus is obviously improvedby using a low-temperature micro-nano wall breaking technology to destroy the cell walls, 2, most of proteins can be separated from the polysaccharides by using an aqueous two-phase extraction technology, to avoid causing influence on the polysaccharides, 3, depigmentation is performed by using macroporous adsorption resin to make the color of the polysaccharide finished product lighter, 4, sodiumcitrate and other inorganic salts mixed in a polysaccharide solution can be removed by using a nanofiltration membrane technology, and the polysaccharide solution is concentrated to avoid the effectof high temperature on polysaccharide activity, and 5, the solvent is safe and pollution-free, the yield is high, and the preparation method is convenient to operate and low in cost.

The invention discloses a process for refining group C/Y/W135 meningococcal polysaccharides. The process includes dissolving one of a coarse group C meningococcal polysaccharide, a coarse group Y meningococcal polysaccharide and a coarse group W135 meningococcal polysaccharide, displacing the polysaccharide into an equilibration buffer solution, feeding a collected polysaccharide to an anion exchange column, using an eluant to elute absorption substances from the anion exchange column, piecewise collecting absorption peaks with wavelength smaller than 206 according to different elution peaks and using a desalting method to remove micromolecule materials. According to the process for refining the group C/Y/W135 meningococcal polysaccharides, protein contents can be effectively controlled, so that the polysaccharide protein content is far lower than the polysaccharide protein content in a cold phenol method, the recovery rate can reach around 80%, and the process is applicable to mass production with 100g of coarse polysaccharide yield per batch.

The invention relates to oyster mushroom galactomannan as well as a preparation method and an application thereof, and belongs to the field of preparation of effective components of edible mushrooms.The pleurotus ostreatus galactomannan is obtained by performing normal-temperature wall-breaking extraction on dried fruiting bodies of pleurotus ostreatus, and combining extrusion separation, proteinremoval and ion exchange chromatography. According to the wall breaking in combination with extrusion filtration, in the preparation method, the extraction time is shortened, the reagent consumptionis reduced, the energy is saved, and the polysaccharide yield is improved; and an adopted extrusion filtration technology is suitable for separation of a viscous solution, and the obtained oyster mushroom galactomannan has the functional activity of protecting myocyte oxidative damage, and can be used for preparing auxiliary medicines and health foods for protecting myocyte oxidative damage.

The invention discloses a preparation method of honey raisin tree polysaccharide. The method comprises the following steps: (1) drying, crushing and degreasing honey raisin tree pulp; (2) extracting honey raisin tree polysaccharide in a vacuum pulsing manner by adopting a complex enzyme preparation, extracting twice, merging extracting solutions, and concentrating in vacuum for later use; (3) carrying out alcohol precipitation on the concentrated honey raisin tree polysaccharide extracting solution, centrifugally separating and drying in vacuum, so as to obtain a honey raisin tree polysaccharide product. The honey raisin tree polysaccharide is extracted by adopting a complex enzyme method, so that the method has the characteristics of short extraction time, high polysaccharide yield, and good storage of active ingredients. Mass transfer is reinforced by vacuum pulsing in the polysaccharide extracting process, so that the efficiency is further improved, and the extraction time is shortened.