Method for extracting polysaccharide of pineapple pomace

A technology of pineapple peel residue and extraction method, which is applied in the directions of antidote, drug combination, etc., can solve the problems of large equipment investment and high operation requirements

Active Publication Date: 2018-05-08
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with hot water extraction, ultrasonic-assisted extraction technology has the advantages of fast, effici

Method used

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  • Method for extracting polysaccharide of pineapple pomace
  • Method for extracting polysaccharide of pineapple pomace
  • Method for extracting polysaccharide of pineapple pomace

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0068] Example 1 Take 5g pineapple peel dry powder, add 150mL distilled water, 1.5% complex enzyme (mass ratio of xylanase: pectinase Y-23=1:2), keep the natural pH of the extract (6.0), and rotate The hydrolysis reaction is carried out in the evaporator, the enzymatic hydrolysis temperature is controlled to 50℃, the enzymatic hydrolysis time is 2h, and the speed is 130rpm / min. After the extraction, the enzyme is inactivated in a boiling water bath for 10min, centrifuged at 8000rpm for 10min, the supernatant is taken, and the residue is added to 150mL of distilled water. It was transferred to a water bath for extraction at 100°C for 2 hours. After the extraction, it was centrifuged at 5000 rpm for 10 minutes, the supernatant was taken, and the residue was extracted once again. Concentrate the volume of the extract to 1 / 5 of the original volume by evaporation under reduced pressure, add 4 times the volume of the concentrate to 95% ethanol, let it stand overnight at 4°C, centrifug...

Example Embodiment

[0069] Example 2 Take 5g of dry powder of pineapple peel residue, add 150mL distilled water, 2.0% complex enzyme (mass ratio of xylanase: pectinase Y-23 = 1:2), keep the natural pH of the extract (6.0), and rotate The hydrolysis reaction is carried out in the evaporator, the enzymatic hydrolysis temperature is controlled at 50℃, the enzymatic hydrolysis time is 1.5h, and the speed is 130rpm / min. After the extraction is completed, the enzyme is inactivated in a boiling water bath, centrifuged at 6000rpm for 10min, the supernatant is taken, and the residue is added to 200mL distilled water Transfer it to a water bath for extraction at 100°C for 2 hours, centrifuge at 5000 rpm for 10 minutes after the end of extraction, take the supernatant, and repeat the extraction of the residue once. Concentrate the volume of the extract to 1 / 5 of the original volume by evaporation under reduced pressure, add 4 times the volume of the concentrate to 95% ethanol, let stand overnight at 4°C, cent...

Example Embodiment

[0075] Embodiment 3 adopts the following steps to realize the present invention:

[0076] (1) Pretreatment: freeze-dried the sieved pineapple skin residue, then pulverize, pass a 40-mesh sieve to obtain dry powder of pineapple skin residue; freeze-drying conditions: temperature -40℃, pressure 6Pa, time 24h;

[0077] (2) Degreasing: Put the dry powder of pineapple peel residue obtained in step (1) in a container, add petroleum ether with a boiling range specification of 30~60°C for reflux extraction 3 times, collect the filter residue, and dry it naturally before use;

[0078] (3) Enzymatic hydrolysis of raw materials: Add distilled water to the pineapple peel powder prepared in step (2) at a material-to-liquid ratio of 1:40g / mL, and at the same time add 2.2% complex enzyme (xylanase: pectinase Y- 23 mass ratio=1:3), under 53℃, pH6.0 water bath conditions, carry out enzymolysis in a rotary evaporator with a rotating speed of 130r / min for 2.1h. After enzymolysis is completed, the enzym...

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Abstract

The invention discloses a method for extracting polysaccharide of pineapple pomace. The method for extracting the polysaccharide of the pineapple pomace comprises the following steps of pretreatment,degreasing, enzymolysis of a raw material, water extraction of filter residues, concentration, alcohol precipitation, deproteinization, depigmentation, drying and the like, wherein the extraction rateof the polysaccharide of the pineapple pomace can be improved effectively by adopting xylanase and pectinase Y-23 as a mixed enzyme preparation, then a trichloroacetic acid-butanol mixed solution isadded for deproteinization of crude polysaccharide, X-5 macroporous adsorption resin is added for the depigmentation of the deproteinized polysaccharide solution, and the purity of the polysaccharideof the pineapple pomace can be improved by controlling the conditions of the steps. The method for extracting the polysaccharide of the pineapple pomace is simple and rapid, and compared with a hot water extraction method, an ultrasonic wave-assisted extraction method and a cellulase extraction method, the obtained polysaccharide of the pineapple pomace has a high extraction rate, high purity andgood antioxidant activity, and can be used for preparing functional food, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of deep processing of horticultural crop products, and in particular relates to a method for extracting pineapple pomace polysaccharide. Background technique [0002] Pineapple (Ananas comosus), also known as pineapple and prosperous pear, is one of the four famous fruits in the tropics. According to incomplete statistics, the planting area of ​​pineapples in tropical regions of my country reaches 67,000 hm 2 , the output reached 1.44 million tons. During the fresh eating and processing of pineapple, a large amount of skin dregs will be produced, accounting for 15-20% of the fruit weight. The proportion of total sugar, water and citric acid is almost the same as that of the pulp. Due to the lack of processing technology and related research, a large amount of skin dregs are produced. Being discarded not only causes waste of resources, but also aggravates environmental pollution. [0003] Polysaccharide is...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003
Inventor 胡会刚赵巧丽谢江辉胡玉林陈晶晶段雅婕
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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