Tremella polysaccharide extracting method

An extraction method and tremella polysaccharide technology are applied in the field of tremella polysaccharide extraction, which can solve the problems of poor economic benefits and insufficient product yield, and achieve the effects of increasing polysaccharide yield, reducing enzyme consumption, and promoting development

Inactive Publication Date: 2016-04-13
赵志鑫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of insufficient product yield and poor economic benefits in extracting polysaccharides from tremella by enzymatic hydrolysis in the prior art, and proposes a new method for extracting tremella polysaccharides. After the enzymolysis, the alkaline extraction step is added, so that the polysaccharide yield of the present invention is more than 16.5%, thereby improving the economic benefits of the edible fungus deep-processing industry

Method used

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Examples

Experimental program
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Embodiment 1

[0021] A method for extracting tremella polysaccharides, comprising crushing, enzymolysis extraction, alkaline extraction, first concentration, protein removal, second concentration, and drying steps, the enzyme used in the enzymolysis step is a compound enzyme, and the compound enzyme It is obtained by mixing protease, cellulase and pectinase at a mass ratio of 0.5:1:2.

Embodiment 2

[0023] A method for extracting tremella polysaccharides, comprising crushing, enzymolysis extraction, alkaline extraction, first concentration, protein removal, second concentration, and drying steps, the enzyme used in the enzymolysis step is a compound enzyme, and the compound enzyme It is obtained by mixing protease, cellulase and pectinase at a mass ratio of 0.5:1:3.

Embodiment 3

[0025] A method for extracting tremella polysaccharides, comprising crushing, enzymolysis extraction, alkaline extraction, first concentration, protein removal, second concentration, and drying steps, the enzyme used in the enzymolysis step is a compound enzyme, and the compound enzyme It is obtained by mixing protease, cellulase and pectinase at a mass ratio of 0.5:1:3.

[0026] In this example, crushing refers to crushing the dried white fungus to 60 meshes; enzymatic extraction refers to mixing white fungus powder and deionized water at a ratio of 1:50, and after adjusting the pH to 6, adding compound enzymes, enzymes After 0.5h of solution, the temperature was raised rapidly to 80°C to inactivate the enzyme, and then placed in a circulating ultrasonic extractor for extraction for 0.5h, and finally filtered to collect the filtrate and filter residue.

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Abstract

The invention discloses a tremella polysaccharide extracting method, and belongs to the technical field of edible fungus deep processing. The method comprises the steps of smashing, enzyme-extraction, alkaline extraction, primary concentration, protein removing, secondary concentration and drying, wherein an enzyme used in the step of enzyme-extraction is a compound enzyme which is obtained by mixing protease, cellulase and pectinase according to the mass ratio of 0.5 to 1 to 2-4. According to the tremella polysaccharide extracting method, by means of the matching design of the compound enzyme and adding the step of alkaline extraction, the yield of polysaccharide is greatly increased, and therefore the economic benefit of an edible fungus deep processing industry is improved.

Description

technical field [0001] The invention relates to a method for extracting Tremella polysaccharide, belonging to the technical field of edible fungus deep processing. Background technique [0002] Tremella fuciformls (Tremella fuciformls) is a kind of medicinal and edible fungus with high economic value and rich nutrition. It has the effects of nourishing body fluid, moistening lung and nourishing stomach. Tremella polysaccharide is the main active ingredient of Tremella, which has the functions of improving the body's immunity, lowering blood sugar, lowering blood fat, anti-aging, anti-ulcer, anti-thrombosis, and anti-mutation. At present, the common extraction method of tremella polysaccharide is hot water extraction, but this traditional extraction method has problems such as low extraction rate, time-consuming, and waste of resources. Tremella polysaccharides are divided into extracellular polysaccharides and cell wall polysaccharides. It is difficult to extract the cell w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 赵志鑫
Owner 赵志鑫
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