Preparation method of Caulis Lonicerae polysaccharide

A technology of honeysuckle vine and polysaccharide, which is applied in the field of medicine, can solve problems such as unmanned access, and achieve the effects of high biological activity, simple operation, and significant economic and social value

Inactive Publication Date: 2012-07-25
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on honeysuckle vine is limited to the research on organic acids, flavonoids, tannins, alkaloids and other compounds, while the research on honeysuckle polysaccharides has no one at home and abroad. At present, research reports have shown that the flower buds of honeysuckle The polysaccharides of honeysuckle have better immunity and antioxidant activity, and are a potential drug in the treatment of tumors and anti-aging.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Dry the honeysuckle vine, crush it through a 20-mesh sieve to obtain the honeysuckle vine powder, weigh 1kg of the honeysuckle vine powder for later use;

[0015] (2) Weigh 5g of cellulase, add 50ml of warm water at 32°C to the cellulase, dissolve, activate in a 32°C water bath for 28 minutes, and obtain an activated enzyme solution for later use;

[0016] (3) Mix the honeysuckle powder weighed above with 10,000 ml of water, soak in a 40°C water bath for 120 minutes, cool to 18°C, adjust the pH to 5.3 with hydrochloric acid, shake well, heat in a water bath to 55°C, and add the activated Enzyme solution, mix well, enzymolysis for 2 hours;

[0017] (4) Extract the solution obtained in step (3) with a water bath at 80-95°C for 2 times, 2 hours each time, filter, combine the filtrates, concentrate to twice the weight and volume of the honeysuckle powder, and then add ethanol to the alcohol content reach 80%, let it stand for 12 hours, centrifuge to remove the supernat...

Embodiment 2

[0020] (1) Dry the honeysuckle vine, crush it through a 20-mesh sieve to obtain the honeysuckle vine powder, weigh 2 kg of the honeysuckle vine powder for later use;

[0021] (2) Weigh 30 g of pectinase, add 450 ml of warm water at 45°C to the pectinase, dissolve it, activate it in a water bath at 45°C for 20 minutes, and obtain an activated enzyme solution for later use;

[0022] (3) Mix the honeysuckle powder weighed above with 30,000 ml of water, soak in a 70°C water bath for 50 minutes, cool to 20°C, adjust the pH to 4 with hydrochloric acid, shake well, heat in a water bath to 47°C, and add the activated enzyme solution, mix well, and enzymatically hydrolyze for 5 hours;

[0023] (4) Extract the solution obtained in step (3) with reflux in a 95°C water bath for 3 times, each time for 5 hours, filter, combine the filtrate, concentrate to 1 times the weight and volume of the honeysuckle powder, and then add ethanol until the alcohol content reaches 80 %, let it stand for 1...

Embodiment 3

[0026] (1) Dry the honeysuckle vine, crush it through a 20-mesh sieve to obtain the honeysuckle vine powder, weigh 3kg of the honeysuckle vine powder for later use;

[0027] (2) Weigh 75g of xylanase, add 1500ml of warm water at 58°C to the xylanase, dissolve it, and activate it in a water bath at 58°C for 8 minutes to obtain an activated enzyme solution for later use;

[0028] (3) Mix the honeysuckle powder weighed above with 60,000 ml of water, soak in 80°C water bath for 20 minutes, cool to 25°C, adjust pH to 4.5 with hydrochloric acid, heat to 40°C in water bath, add the above activated enzyme solution, mix homogeneous, enzymatic hydrolysis for 11 hours;

[0029] (4) Extract the solution obtained in step (3) with reflux in a 95°C water bath for 4 times, each time for 3 hours, filter, combine the filtrate, concentrate to 1 times the weight and volume of the honeysuckle powder, and then add ethanol until the alcohol content reaches 80 %, let it stand for 12 hours, centrifug...

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PUM

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Abstract

The invention relates to a preparation method of Caulis Lonicerae polysaccharide, effectively solving the preparation problem of the Caulis Lonicerae polysaccharide. The preparation method comprises the following technical scheme: drying and crushing Caulis Lonicerae for later use; adding water to enzyme, dissolving, activating by a water bath to obtain an activated enzyme solution for later use; mixing Caulis Lonicerae powder with water, carrying out water bath, soaking, adjusting the pH to be 4.0-5.5, uniformly shaking, heating with water bath, adding the activated enzyme solution, mixing uniformly, carrying out enzymolysis, reflowing by water bath and extracting, filtering, condensing, adding ethanol till the ethanol content reaches 80%, standing, and centrifuging to remove supernate to obtain a polysaccharide precipitate; and sequentially adding anhydrous ethanol, acetone and aether to the polysaccharide precipitate, washing, centrifuging to remove supernate, drying the precipitate in vacuum to obtain the Caulis Lonicerae polysaccharide. The preparation method disclosed by the invention is simple, convenient and quick to operate and high in polysaccharide yield; initial reactants are available and low in price; and the preparation cost is greatly reduced, and the extracted Caulis Lonicerae polysaccharide is high in purity, keeps high biological activity and has remarkable economic and social values.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of honeysuckle polysaccharide. Background technique [0002] Casulis Lonicerae, a traditional Chinese medicine, is the Lonicerae Lonicerae plant. onicera japonica The dried stems and branches of the plant are rich in resources, cheap and easy to get. Traditional Chinese medicine is used to treat fever due to febrile disease, toxic heat and bloody dysentery, carbuncle and sore, rheumatism-heat arthralgia, red, swollen and hot pain in joints. There is heat-clearing and toxic substances removing, the function of dispelling wind and dredging collaterals. At present, the research on honeysuckle vine is limited to the research on organic acids, flavonoids, tannins, alkaloids and other compounds, while the research on honeysuckle polysaccharides has no one at home and abroad. At present, research reports have shown that the flower buds of honeysuckle The polysaccharides o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/00A61P37/02A61P39/06
Inventor 刘富岗杨云卫冰许闽
Owner HENAN UNIV OF CHINESE MEDICINE
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