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Complex enzymatic extraction method for laminarin and obtained laminarin

A technology of laminar polysaccharides and extraction methods, which is applied in the field of compound enzyme extraction methods and obtained laminar polysaccharides, can solve the problems of long time consumption, extraction rate, low purity and activity, and pollute the environment, and achieve the effect of improving the yield of polysaccharides

Inactive Publication Date: 2017-06-23
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a compound enzyme extraction method for laminar polysaccharides in view of the shortcomings of the existing laminar polysaccharide extraction process, such as low extraction rate, purity, and activity, or polluting the environment, and taking a long time. The polysaccharides in kelp are designed to increase the yield and purity of polysaccharides under mild conditions, and to fully retain the polysaccharide structure and biological activity

Method used

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  • Complex enzymatic extraction method for laminarin and obtained laminarin
  • Complex enzymatic extraction method for laminarin and obtained laminarin
  • Complex enzymatic extraction method for laminarin and obtained laminarin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Kelp processing leftovers are used as raw materials, first dried and crushed, take an appropriate amount of kelp powder, add it to a fully automatic hydrolysis reactor, add a certain volume (solid-liquid ratio 1g: 35ml) of 0.1mol / L, pH6.0 citric acid-sodium citrate Buffer solution, stir well and then add compound enzyme. According to the mass of kelp powder is 1g, the amount of cellulase added is 0.004g, hemicellulase 0.006g, pectinase 0.004g and protease 0.01g;

[0046] Adjust the pH value to 5.5, 45°C, enzymatic hydrolysis for 3 hours, heat up to inactivate the enzyme, take out the hydrolyzate, filter to remove precipitates and insoluble matter, add ethanol to the supernatant until the volume fraction of ethanol in the system is 60%, refrigerate at 4°C overnight . Centrifuge at 3000g / min for 20min, and freeze-dry the precipitate to obtain kelp crude polysaccharide. The crude polysaccharide was formulated into a 25% aqueous solution, and ethanol was added until the et...

Embodiment 2

[0048] Kelp processing leftovers are used as raw materials, first dried and crushed, take an appropriate amount of kelp powder, add it to a fully automatic hydrolysis reactor, add a certain volume (solid-liquid ratio 1g: 40ml) of 0.1mol / L, pH6.0 citric acid-sodium citrate Buffer solution, stir well and then add compound enzyme. According to the mass of kelp powder is 1g, the amount of cellulase added is 0.005g, hemicellulase 0.007g, pectinase 0.005g and protease 0.012g;

[0049] Adjust the pH value to 6.0, 50°C, enzymatic hydrolysis for 3.5h, increase the temperature to inactivate the enzyme, take out the hydrolyzate, remove the precipitate and insoluble matter by suction filtration, add ethanol to the supernatant until the volume fraction of ethanol in the system is 65%, refrigerate at 4°C, overnight. Centrifuge at 3500g / min for 20min, and freeze-dry the precipitate to obtain kelp crude polysaccharide. Prepare a 30% aqueous solution of the crude polysaccharide, add ethanol u...

Embodiment 3

[0051] Kelp processing leftovers are used as raw materials, first dried and crushed, take an appropriate amount of kelp powder, add it to a fully automatic hydrolysis reactor, add a certain volume (solid-liquid ratio 1g: 45ml) of 0.1mol / L, pH6.0 citric acid-sodium citrate Buffer solution, stir well and then add compound enzyme. According to the mass of kelp powder is 1g, the amount of cellulase added is 0.006g, hemicellulase 0.008g, pectinase 0.006g and protease 0.014g;

[0052] Adjust the pH value to 6.5, 55°C, enzymatic hydrolysis for 4 hours, increase the temperature to inactivate the enzyme, take out the hydrolyzate, remove the precipitate and insoluble matter by suction filtration, add ethanol to the supernatant until the volume fraction of ethanol in the system is 70%, refrigerate at 4°C overnight . Centrifuge at 4000g / min for 20min, and freeze-dry the precipitate to obtain kelp crude polysaccharide. Prepare a 35% aqueous solution of the crude polysaccharide, add ethano...

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Abstract

The invention discloses a complex enzymatic extraction method for laminarin and the obtained laminarin. The method comprises the following steps: taking kelp processing waste as a raw material, drying and crushing the kelp processing waste first, extracting and adding a proper amount of kelp powder into a fully-automatic hydrolysis reactor, adding a buffer solution with pH 6.0 for uniform stirring according to a solid-liquid ratio of 1:(35-45)ml, and adding complex enzymes, the additions of cellulase, hemicellulase, pectinase and protease being 0.004 to 0.006 grams, 0.006 to 0.008 grams, 0.004 to 0.006 grams and 0.001 to 0.014 grams based on the mass of 1 gram of the kelp powder; regulating the pH value to be 5.5 to 6.5, performing enzymatic hydrolysis for 3 to 4h at 45 to 55 DEG C, raising the temperature for enzyme deactivation, extracting hydrolysate, performing suction filtration to remove precipitates and insoluble substances, adding ethanol into supernatant until the volume fraction of the ethanol of the system is 65 to 75 percent, and performing cold storage overnight in a refrigerator of 4 DEG C; performing centrifugation for 20min at 3,000 to 4,000g / min, and performing freeze-drying on precipitates to obtain crude laminarin. According to the method and the laminarin, the yield and purity of the laminarin can be effectively improved under a mild condition, the structure and bioactivity of the laminarin can be completely preserved, and toxic substances and environmental pollution in a process are avoided.

Description

technical field [0001] The invention relates to laminarin and its extraction method, specifically, a compound enzyme extraction method of laminarin and the obtained laminarin. Background technique [0002] my country's kelp production ranks first in the world. At present, the kelp processing industry mainly focuses on rough processing; the brown algae industry mainly produces sodium alginate, mannitol and iodine. The brown algae industry and the rough processing of kelp produce a large amount of residue, waste liquid and leftovers. Fucoidan, seaweed protein and dietary fiber exist in it, which not only pollutes the environment and wastes resources, but also is a huge waste. A large number of studies have found that laminarin has various physiological activities such as anti-oxidation, anti-tumor, anti-virus, anti-coagulation, and lowering blood pressure, and can improve the body's immunity. To find out a method for efficiently extracting laminarin polysaccharides from kelp...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 付学军孙力赵明日王鸷丁宁
Owner YANTAI UNIV
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