Complex enzymatic extraction method for laminarin and obtained laminarin

A technology of laminar polysaccharides and extraction methods, which is applied in the field of compound enzyme extraction methods and obtained laminar polysaccharides, can solve the problems of long time consumption, extraction rate, low purity and activity, and pollute the environment, and achieve the effect of improving the yield of polysaccharides

Inactive Publication Date: 2017-06-23
YANTAI UNIV
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a compound enzyme extraction method for laminar polysaccharides in view of the shortcomings of the existing laminar polysaccharide extraction process, such as low extraction rate, pu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Complex enzymatic extraction method for laminarin and obtained laminarin
  • Complex enzymatic extraction method for laminarin and obtained laminarin
  • Complex enzymatic extraction method for laminarin and obtained laminarin

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1

[0045] The leftovers of kelp processing are used as raw materials, dried and crushed first, take an appropriate amount of kelp powder, add it to a fully automatic hydrolysis reactor, add a certain volume (1g: 35ml) of 0.1mol / L, pH 6.0 citric acid-sodium citrate Buffer, mix well and add compound enzyme based on the mass of kelp powder as 1g, the added amount of cellulase is 0.004g, hemicellulase 0.006g, pectinase 0.004g and protease 0.01g;

[0046] Adjust the pH value to 5.5, 45℃, enzymatic hydrolysis for 3h, raise the temperature to kill the enzyme, take out the hydrolysate, suction and filter to remove the precipitate and insoluble matter, add ethanol to the supernatant until the ethanol volume fraction of the system is 60%, refrigerate at 4℃, overnight . Centrifuge at 3000g / min for 20min, and freeze-dry the sediment to obtain kelp crude polysaccharide. The crude polysaccharide was prepared into a 25% aqueous solution, ethanol was added to the system to achiev...

Example Embodiment

[0047] Example 2

[0048] The leftovers from kelp processing are used as raw materials, dried and crushed first, take an appropriate amount of kelp powder, add it to a fully automatic hydrolysis reactor, add a certain volume (1g: 40ml) of 0.1mol / L, pH6.0 citric acid-sodium citrate Buffer, mix well and add compound enzyme based on the mass of kelp powder as 1g, the added amount of cellulase is 0.005g, hemicellulase 0.007g, pectinase 0.005g and protease 0.012g;

[0049] Adjust the pH to 6.0, 50℃, enzymatic hydrolysis for 3.5h, raise the temperature to kill the enzyme, take out the hydrolysate, suction and filter to remove the precipitate and insoluble matter, add ethanol to the supernatant until the ethanol volume fraction of the system is 65%, and refrigerate at 4℃. overnight. Centrifuge at 3500g / min for 20min and freeze-dry the sediment to obtain kelp crude polysaccharide. The crude polysaccharide was prepared into a 30% aqueous solution, ethanol was added to the system to have a...

Example Embodiment

[0050] Example 3

[0051] The leftovers from kelp processing are used as raw materials, dried and crushed first, and an appropriate amount of kelp powder is taken and added to a fully automatic hydrolysis reactor. Add a certain volume (1g:45ml) of 0.1mol / L, pH6.0 citric acid-sodium citrate Buffer solution, after mixing, add compound enzyme according to the mass of kelp powder as 1g, the added amount of cellulase is 0.006g, hemicellulase 0.008g, pectinase 0.006g and protease 0.014g;

[0052] Adjust the pH to 6.5, 55℃, enzymatic hydrolysis for 4h, raise the temperature to kill the enzyme, take out the hydrolysate, suction filter to remove the precipitate and insoluble matter, add ethanol to the supernatant until the ethanol volume fraction of the system is 70%, refrigerate at 4℃ in the refrigerator, overnight . Centrifuge at 4000g / min for 20min, and freeze-dry the sediment to obtain kelp crude polysaccharide. The crude polysaccharide was prepared into a 35% aqueous solution, ethano...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a complex enzymatic extraction method for laminarin and the obtained laminarin. The method comprises the following steps: taking kelp processing waste as a raw material, drying and crushing the kelp processing waste first, extracting and adding a proper amount of kelp powder into a fully-automatic hydrolysis reactor, adding a buffer solution with pH 6.0 for uniform stirring according to a solid-liquid ratio of 1:(35-45)ml, and adding complex enzymes, the additions of cellulase, hemicellulase, pectinase and protease being 0.004 to 0.006 grams, 0.006 to 0.008 grams, 0.004 to 0.006 grams and 0.001 to 0.014 grams based on the mass of 1 gram of the kelp powder; regulating the pH value to be 5.5 to 6.5, performing enzymatic hydrolysis for 3 to 4h at 45 to 55 DEG C, raising the temperature for enzyme deactivation, extracting hydrolysate, performing suction filtration to remove precipitates and insoluble substances, adding ethanol into supernatant until the volume fraction of the ethanol of the system is 65 to 75 percent, and performing cold storage overnight in a refrigerator of 4 DEG C; performing centrifugation for 20min at 3,000 to 4,000g/min, and performing freeze-drying on precipitates to obtain crude laminarin. According to the method and the laminarin, the yield and purity of the laminarin can be effectively improved under a mild condition, the structure and bioactivity of the laminarin can be completely preserved, and toxic substances and environmental pollution in a process are avoided.

Description

technical field [0001] The invention relates to laminarin and its extraction method, specifically, a compound enzyme extraction method of laminarin and the obtained laminarin. Background technique [0002] my country's kelp production ranks first in the world. At present, the kelp processing industry mainly focuses on rough processing; the brown algae industry mainly produces sodium alginate, mannitol and iodine. The brown algae industry and the rough processing of kelp produce a large amount of residue, waste liquid and leftovers. Fucoidan, seaweed protein and dietary fiber exist in it, which not only pollutes the environment and wastes resources, but also is a huge waste. A large number of studies have found that laminarin has various physiological activities such as anti-oxidation, anti-tumor, anti-virus, anti-coagulation, and lowering blood pressure, and can improve the body's immunity. To find out a method for efficiently extracting laminarin polysaccharides from kelp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 付学军孙力赵明日王鸷丁宁
Owner YANTAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap