Preparation method of honey raisin tree polysaccharide

The technology of jujube jujube and polysaccharide is applied in the field of compound enzyme preparation enzymolysis and vacuum pulse extraction to prepare jujube jujube polysaccharide, which can solve the problems of not meeting the requirements of extraction of jujube jujube polysaccharide, easily destroying the specific structure of polysaccharide, and taking a long time. To achieve the effect of shortened extraction time, high polysaccharide yield and short time

Inactive Publication Date: 2014-04-02
JISHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The predecessors used neutral hot water extraction or acid and alkali extraction to extract the polysaccharides of jujube. The former takes a long time and the yield is low, and the latter is easy to destroy the specific structure related to the activity of the polysaccharide.
Therefore, the prior art cannot meet the requirements for the extraction of jujube polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Treatment of jujube: Dry, pulverize and degrease the pulp of jujube.

[0021] (2) Extraction of jujube polysaccharide: put the material in the extraction tank, add water 10 times the mass of the material, 1.15% cellulase, 1.48% pectinase, 0.98% papain, of which the cellulose The enzyme activity is 29×10 4 U·mg -1 , the enzyme activity of pectinase is 1.2×10 4 U·mg -1 , the activity of papain is 101×10 4 U·mg -1 , adjust the pH value to 3.6, and the temperature is 45°C, extract for 61 minutes, filter and repeat the operation once, combine the filtrate obtained twice, concentrate in vacuo and set aside.

[0022] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses are used to enhance mass transfer.

[0023] (3) Analysis and drying of jujube polysaccharide: filter the concentrated jujube polysaccharide extract, and add ethanol to make the concentration of ethanol 58%. To obtain the jujube polysaccha...

Embodiment 2

[0025] (1) Treatment of jujube: Dry, pulverize and degrease the pulp of jujube.

[0026] (2) Extraction of jujube polysaccharides: put the material in the extraction tank, add water 6 times the mass of the material, 0.81% cellulase, 1.33% pectinase, 1.15% papain, of which the cellulose Enzyme activity is 21×10 4 U·mg -1 , the enzyme activity of pectinase is 1.9×10 4 U·mg -1 , the activity of papain is 82×10 4 U·mg -1 , adjust the pH value to 4.1, the temperature is 41 ° C, extract for 32 minutes, filter and repeat the operation once, combine the filtrate obtained twice, concentrate in vacuo and set aside.

[0027] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses are used to enhance mass transfer.

[0028] (3) Analysis and drying of jujube polysaccharide: filter the concentrated jujube polysaccharide extract, and add ethanol to make the concentration of ethanol 66%. To obtain the jujube polysaccharide pr...

Embodiment 3

[0030] (1) Treatment of jujube: Dry, pulverize and degrease the pulp of jujube.

[0031] (2) Extraction of jujube polysaccharides: put the material in the extraction tank, add water 14 times the mass of the material, 1.02% cellulase, 1.67% pectinase, 0.83% papain, of which the cellulose Enzyme activity is 38×10 4 U·mg -1 , the enzyme activity of pectinase is 2.7×10 4 U·mg -1 , the activity of papain is 117×10 4 U·mg -1 , adjust the pH value to 4.4, the temperature is 49 ° C, extract for 87 minutes, filter and repeat the operation once, combine the filtrate obtained twice, concentrate in vacuo and set aside.

[0032] (3) Analysis and drying of jujube polysaccharide: filter the concentrated jujube polysaccharide extract, and add ethanol to make the concentration of ethanol 78%. To obtain the jujube polysaccharide product, the mass fraction of the polysaccharide in the product is 55%.

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Abstract

The invention discloses a preparation method of honey raisin tree polysaccharide. The method comprises the following steps: (1) drying, crushing and degreasing honey raisin tree pulp; (2) extracting honey raisin tree polysaccharide in a vacuum pulsing manner by adopting a complex enzyme preparation, extracting twice, merging extracting solutions, and concentrating in vacuum for later use; (3) carrying out alcohol precipitation on the concentrated honey raisin tree polysaccharide extracting solution, centrifugally separating and drying in vacuum, so as to obtain a honey raisin tree polysaccharide product. The honey raisin tree polysaccharide is extracted by adopting a complex enzyme method, so that the method has the characteristics of short extraction time, high polysaccharide yield, and good storage of active ingredients. Mass transfer is reinforced by vacuum pulsing in the polysaccharide extracting process, so that the efficiency is further improved, and the extraction time is shortened.

Description

technical field [0001] The invention relates to a method for preparing jujube polysaccharides, in particular to a method for preparing jujube polysaccharides by adopting compound enzyme preparation enzymolysis and vacuum pulsation extraction. Background technique [0002] Polysaccharides are macromolecular substances in natural organic compounds, which have health care and medicinal value. For example, lentinan has anti-tumor and immune-enhancing effects. Many important physiological processes are closely related to polysaccharide molecules. These have attracted attention at home and abroad. attention of scientists. [0003] Jujube has the effects of clearing heat and diuresis, relieving drunkenness, curing rheumatism, quenching thirst, reducing fire and detoxification, etc. Most of these effects are related to the polysaccharide in Jujube, which makes the extraction of Jujube polysaccharide have important practical value. The predecessors used neutral hot water leaching or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 黄群杨万根麻成金王珏揭雅娇
Owner JISHOU UNIVERSITY
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