112results about How to "Activity is not affected" patented technology

The invention relates to a method for preparing a chitosan oligosaccharide by applying a complex enzyme. The complex enzyme comprises cellulose, lysozyme, amylase, lipase, glucolase and papain. The invention further provides a method for preparing a chitosan oligosaccharide by applying the complex enzyme. The method comprises the following step of: adding the complex enzyme into a reaction buffersolution of chitosan, wherein the mass ratio of the complex enzyme to the chitosan is (1-20):100, and is preferably (5-15):100. The complex enzyme and the chitosan oligosaccharide prepared from the complex enzyme have uniform molecular weight distribution and high yields.

The invention relates to a sewage treatment composite gel material for microbe embedding, and a preparation method thereof. The sewage treatment composite gel material for microbe embedding comprises, by weight, 60-80 parts of carrageenan, 5-15 parts of polyacrylamide, 5-8 parts of porous starch, 4-8 parts of microbes, 0.1-0.3 parts of a growth factor, 1-4 parts of inorganic powder, 1-3 parts of a cross-linking agent and 0.5-0.8 parts of a potassium salt. The composite gel material is prepared by adopting carrageenan and the polyacrylamide composite gel as an embedding agent, the porous starch as an adsorbent and a carrier and the inorganic powder as an additive, is used for embedding microbes, is applied to sewage treatment, can effectively adsorb and remove heavy metal ions and degrade organic matters, and has the advantages of simple preparation process, low cost and market application prospect.

The invention relates to a method for synthesizing caproic acid by catalyzing lactic acid through microorganisms. According to the method, specific functional florae are adopted to biologically synthesize caproic acid from lactic acid in a culture liquid or wastewater. By adopting the method that lactic acid is adopted as the electron donor for producing caproic acid, the efficiency is increased by more than 20% when being compared with that of a conventional method that ethanol is adopted as an electron donor for producing caproic acid, the toxicity resistance concentration of the caproic acid functional bacterium to metabolites (caproic acid) is increased by more than 2 times when being compared with that of reported caproic acid bacteria, and the caproic acid functional bacterium has the characteristics of high conversion rate and high stress resistance. When the lactic acid as a non-fuel substance which is easy to obtain is converted into caproic acid, the conversion efficiency is high, and the high industrial practicability is achieved.

The invention provides a complete premixed multiple primer-specific PCR detection kit and method for detecting nucleotide polymorphisms at three sites of c.677 and c.1298 of the folic acid metabolic pathway and c.1298 of the MTRR gene. "Allele-specific PCR" principle, design multiple primer-specific PCR, realize two-tube reaction on the real-time fluorescent quantitative PCR technology platform, and simultaneously detect three folic acid metabolic pathway MTHFR genes c.677 and c.1298 and MTRR gene c.66 The nucleotide type of the site. In addition, modified Taq DNA polymerase and disaccharide PCR protective agent are added to the reaction system to increase the stability of the reaction system and realize the long-term stability of the pre-mixed detection system.

The invention discloses a heparin affinity column as well as the preparation method and the application thereof. The heparin affinity column is prepared through the following method: agarose gel 6FF is taken as a solid phase carrier which is firstly activated and then is aminated and coupled with amidocyanogen after an epoxy group is coupled, the aminated agarose gel 6FF and the heparin form intermediate aldimine in methanol, and the intermediate product of aldimine is further subjected to reductive amination for forming stable chemical bonds, thereby acquiring the heparin-agarose gel 6FF. The affinity column preparing preparation with high efficiency is simple and short. Through adopting the combination of the rear aldehyde of the heparin and the amidocyanogen of aminated sepharose, the activity of the heparin is not influenced, thereby ensuring the affinity column to have higher affinity; the heparin coupling is stable, and the heparin can be repeatedly used; the reagent adopted by the method has low cost and no environmental pollution, thereby laying a foundation for sweepingly isolating and purifying materials with specific binding capacity to the heparin in common laboratories, and promoting the research on domestic relative fields.

The invention discloses a microfluidic chip integrating circulating tumor cell separation and single cell immunoblotting. The microfluidic chip is characterized by comprising a circulating tumor cellsorting unit, a single cell capture unit, a photoactive gel electrophoresis separation unit and an immunoblotting analysis unit, wherein the circulating tumor cell sorting unit is used for separatingand sorting circulating tumor cells; the single cell capture unit is used for capturing single cells of sorted and enriched cells and performing closed lysis on the cells; the photoactive gel electrophoresis separation unit is used for pushing a protein obtained after cell lysis to a gel coating area on a chip; and the immunoblotting analysis unit is used for performing incubation and elution through a specific antibody, performing fluorescent or luminescent group labeling on a target protein molecule and detecting a signal of the target protein molecule. According to the invention, a varietyof functional units of cell sorting, purification, single cell capture, gel electrophoresis, immunoblotting analysis and the like of the circulating tumor cells are integrated on the micro-fluidic chip, so the separation speed, the analysis speed and the automation degree of the circulating tumor cells are greatly improved.

The invention provides an animal model with male reproductive disorders of a non-human mammalian animal, as well as a preparation method and application of the animal model, and further identifies the functions of a Prss37 gene and a protein. The Prss37 gene of the animal model is inactivated, thereby affecting the appearance of ADAM3 in mature sperms produced by the animal model, indirectly affecting the combination of the sperms-zona pellucida and the migration of the sperms from the uterus to the oviduct and resulting in the serious male reproductive disorders. The model, the Prss37 gene and the protein thereof can be used for screening ADAM3 ripening accelerators, as well as sterility infertility treatment agents or contraceptive medicaments.

The present invention discloses one kind of adjuvant for pig vaccine and its preparation process and constituted pig vaccine. The gene adjuvant for pig vaccine is adjuvant including pig gamma-interferon gene (pIFN-gamma), or recombinant plasmid pcDNA-pIFN-gamma of animal cell expression plasmid pcDNA3.1 and pig gamma-interferon gene (pIFN-gamma), or the combination of pcDNA-pIFN-gamma and No. 206 adjuvant. The pig vaccine is composition of pigí»s cysticercus-resisting vaccine composition; pigí»s deactivated foot-and-mouth disease virus vaccine and the gene adjuvant. The gene adjuvant of the present invention is prepared through gene cloning, recombination, recombinant plasmid proliferation, extraction and purification and other processes.

The invention relates to a preparation method of placental-chorionic-plate-tissue-derived mesenchymal stem cells, and belongs to the technical field of cell biology. The preparation method of the placental-chorionic-plate-tissue-derived mesenchymal stem cells comprises the following steps: carrying out tissue separation, namely to obtain a placenta tissue sample, separate out chorion tissue, and carry out washing with a tissue cleaning solution until the chorion tissue is semi-translucent; carrying out tissue cryopreservation, namely to put the washed chorion tissue into a cryopreservation solution, and carry out cooling and cryopreservation according to predetermined procedures; carrying out tissue treatment, namely to take out the chorion tissue after the cryopreservation, soak the chorion tissue with ethanol, remove impurities, and perform shearing so as to obtain tissue masses; and then, carrying out cell culture, namely to put the tissue masses into a culture flask, add a mesenchymal stem cell selection medium so as to obtain a mixture, put the mixture into a CO(2) incubator so as to carry out incubation until cell fusion degree reaches 80+/-10%, carry out cell passage, and continue amplification culture and/or cryopreservation of the passage cells. The preparation method of the placental-chorionic-plate-tissue-derived mesenchymal stem cells enables long-term storage of placental chorionic plate tissue and mesenchymal stem cells separated from the placental-chorionic plate tissue in liquid nitrogen; and moreover, the placental chorionic plate tissue and the mesenchymalstem cells still have maintained cell activity after reconstruction.

The invention discloses a C reaction protein immune turbidimetry kit which comprises a freeze-drying R1 reagent and a freeze-drying R2 reagent which are fixed in the same container, wherein the freeze-drying R1 reagent and the freeze-drying R2 reagent are respectively formed by adding an excipient and a protective agent in a liquid R1 reagent and a liquid R2 reagent for freeze drying, wherein the liquid R1 reagent is a buffering liquid containing PEG; the liquid R2 reagent is an anti-human C reaction protein coupled polyclonal antibody or monoclonal antibody coupled latex, an anti-human C reaction protein type polyclonal antibody or monoclonal antibody coupled colloidal gold, or anti-human C reaction protein antiserum. According to the kit, the detection is convenient to operate, the time is saved, the transportation and the storage are convenient, the transportation problem in cold regions is solved, and the stability of the reagents is enhanced.

The present invention discloses one kind of adjuvant for pig vaccine and its preparation process and constituted pig vaccine. The gene adjuvant for pig vaccine is adjuvant including pig interleukin-6 gene (pIL-6), or recombinant plasmid pcDNA-pIL-6 of animal cell expression plasmid pcDNA3.1 and pig interleukin-6 gene (pIL-6). The pig vaccine is composition of pigí»s cysticercus-resisting vaccine composition; pigí»s deactivated foot-and-mouth disease virus vaccine and the gene adjuvant. The gene adjuvant of the present invention is prepared through gene cloning, recombination, recombinant plasmid proliferation, extraction and purification and other processes.

The invention discloses a method for preparing a liquid compound enzyme. The method comprises the steps that: strain is selected to carry out solid fermentation on materials; the solid fermentation materials are smashed and added with a certain amount of water for blending and wetting; then filtrate and dregs are obtained by pressing the mixture; after the dregs are dried, solid feed enzyme is obtained; the pressed filtrate is filtered, then antisepticise stabilizing agent is added in filter clogging liquid to obtain liquid compound enzyme used in fodder. The application of the method ensures that all enzymes in the prepared liquid compound enzyme are mutual complement, independent, has non-antagonism and the activity is not affected; moreover, the cost of the liquid compound enzyme can be reduced.

The invention relates to an automatic control device and method for achieving low-concentration ammonia-nitrogen wastewater short-range nitration. The device is composed of the five parts of a short-range nitration reactor, a water inlet system, a water outlet system, a water bath circulating system and an automatic control system. An on-line monitoring device is adopted, according to the concentration of ammonia-nitrogen in the short-range nitration reactor, reasonable aeration is conducted, it is guaranteed that the activity of ammonia-oxidizing bacteria is not influenced, the short-range nitration efficiency is improved, and nitrite can also be prevented from being further oxidized into nitrate nitrogen; meanwhile, the hydraulic retention time of the short-range nitration reactor can beprecisely controlled, the occurrence that when the concentration of ammonia-nitrogen in outlet water is too low, requirements of an anaerobic ammonia-oxidizing matrix cannot be met is avoided, the possibility that when the hydraulic retention time is too long, nitrite is further oxidized into nitrate nitrogen can also be reduced to a certain degree, and it is also guaranteed that the pH value anddissolved oxygen of the short-range nitration reactor are in the optimal range.

The invention relates to a recombinant beta-glucosidase and an expression and purification method and the immobilization application thereof, and belongs to the technical field of biological enzyme engineering. The recombinant beta-glucosidase is recombinant beta-glucosidase Glu-linker-ELP-GB (GLEGB) obtained by linking binary labels ELP and GB with beta-glucosidase (Glu) through a universal linking peptide linker, wherein the sequence of GB polypeptide is linked to the 3' end of an ELP gene. By virtue of a reversible phase change property of an ELP label on recombinant enzyme, separation andpurification of the enzyme can be achieved; the recombinant enzyme containing the ELP label can be precipitated only by adding a certain amount of (HN4)2SO4 for incubation for a period of time; by virtue of a temperature response behavior of the ELP label, one-step rapid separation and purification of a target enzyme molecule is achieved; the enzyme purifying effect of the expression and purification method is superior to that of an ammonium sulfate precipitation method, and the purification cost of the expression and purification method is much lower than that of a chromatography method.

A preparation method for a feeding solid complex enzyme comprises the following contents of: a. drying solid fermented materials by low temperature flash evaporation, and crashing the materials to prepare the raw powder; b. adding auxiliary ingredients to the raw powder according to the prescription to prepare the feeding solid complex enzyme; and c. packaging and storing the finished product. Inthe prescription, the auxiliary ingredients include acid protease, one or two types of alpha-amylases, and corn bran powder. The ratio of the components by weight is as follows: 4-12 percent of raw powder, 0 or 1-6 percent of 50000U/g acid protease, 0 or 4-5 percent of 2000U/g alpha-amylase, and the balance of corn bran powder. The solid complex enzyme prepared by the method has the advantages oflow cost, complete zymogram and high enzyme activity. Furthermore, the enzymes are mutually supplementary and dependent without antagonism.

The invention provides a hydrogel vascular micro stent and a preparation method thereof. The preparation method comprises the following steps that (a), 3D printing of a mixed solution of GelMA hydrogel and photoinitiator is carried out to obtain GelMA hydrogel tablet; and (b), the GelMA hydrogel tablet obtained from step (a) is self-coiled in imitation of apple peel to obtain the hydrogel vascularmicro stent. Compared with the prior art, the preparation method, different from the previous 3D printing, constructs the hydrogel vascular micro stent in the self-crimping way by imitating the applepeel and can construct a more fine micro-hollow tube (less than 200 [mu]m),the hydrogel vascular micro stent can first construct gel sheets of different shapes and sizes, and then obtain gel tubes ofcorresponding forms with a higher accuracy and controllability; and moreover, the hydrogel vascular micro stent obtained by the preparation method has good cell affinity, good biocompatibility and rich forms, the bionic need of all forms of vascular stents can be basically met, and great development prospects can be reached.

The invention relates to an oxygen-resistant molybdenum phosphide catalyst, which contains sulfur, wherein the sulfur is distributed on the surface and the bulk phase of molybdenum phosphide. According to the present invention, the disclosed molybdenum phosphide catalyst has excellent oxygen resistance, the catalytic activity of the oxygen-resistant molybdenum phosphide catalyst is not substantially affected after the oxygen-resistant molybdenum phosphide catalyst is stored in the air for a long time, and the oxygen-resistant molybdenum phosphide catalyst does not require the pre-reduction activation when the oxygen-resistant molybdenum phosphide catalyst is used in hydrogenation, hydrodesulfurization, hydrodenitrogenation and other hydrogen present reactions.

The invention discloses a washing device. The washing device comprises a gun body, a first high-temperature steam pipe, a valve device and an extraction liquid pipe, wherein the gun body comprises a stock and gun barrel segments communicated with the stock, the gun barrel segments include the first gun barrel segment, the second gun barrel segment and the third gun barrel segment, one end of the first gun barrel segment is communicated with one end of the stock, the other end of the first gun barrel segment is communicated with one end of the second gun barrel segment through a universal joint with a hollow cavity, the second gun barrel segment is sleeved with the third gun barrel segment, one end of the third gun barrel segment is connected with the universal joint, an annular space is formed by the end of the third gun barrel segment and the universal joint, and the other end of the third gun barrel segment extends to the front side of the other end of the second gun barrel segment to form a diffusion space; the first high-temperature steam pipe is arranged in the gun body, one end of the first high-temperature steam pipe is communicated with one end of the universal joint, and the other end of the first high-temperature steam pipe extends out of the other end of the stock to be communicated with a high-temperature high-pressure steam generation device; the valve device is arranged between the stock and the first gun barrel segment and used for connecting and disconnecting the first high-temperature steam pipe; the extraction liquid pipe enters the first gun barrel segment and is communicated with the first high-temperature steam pipe and the universal joint.

The invention discloses a preparation method of honey raisin tree polysaccharide. The method comprises the following steps: (1) drying, crushing and degreasing honey raisin tree pulp; (2) extracting honey raisin tree polysaccharide in a vacuum pulsing manner by adopting a complex enzyme preparation, extracting twice, merging extracting solutions, and concentrating in vacuum for later use; (3) carrying out alcohol precipitation on the concentrated honey raisin tree polysaccharide extracting solution, centrifugally separating and drying in vacuum, so as to obtain a honey raisin tree polysaccharide product. The honey raisin tree polysaccharide is extracted by adopting a complex enzyme method, so that the method has the characteristics of short extraction time, high polysaccharide yield, and good storage of active ingredients. Mass transfer is reinforced by vacuum pulsing in the polysaccharide extracting process, so that the efficiency is further improved, and the extraction time is shortened.

The invention discloses a preparation method for portobello mushroom polysaccharide with immunoregulatory activity in order to be suitable for modern large-scale production. The preparation method comprises the following steps: performing microwave extraction on portobello mushroom powder; cooling the portobello mushroom powder to room temperature; removing impurities through macroporous adsorption resin, then adjusting the pH value to be 3.0-10.0, and performing deproteinization by connecting anion and cation exchange resin in series with a resin column; performing further purification on the portobello mushroom polysaccharide subjected to impurity removal and deproteinization by an ultrafiltration technology, wherein the retained molecular weight in a purification process is 100,000-800,000 D; pre-freezing the portobello mushroom polysaccharide ultrafiltrate until water in the material is completely frozen, and performing vacuum drying to obtain the portobello mushroom polysaccharide. According to the method, the microwave extraction technology, the resin series connection impurity removal and deproteinization technology and the ultrafiltration technical purification and freeze-drying technology are comprehensively applied, so that the purity of the obtained portobello mushroom polysaccharide can be up to over 90 percent, and an obvious immunoregulation effect can be achieved.