Oyster mushroom galactomannan as well as preparation method and application thereof

A technology of Pleurotus ostreatus galactomanna and mannaglucose, which is applied in the fields of application, pharmaceutical formulations, polysaccharide/gum-containing food ingredients, etc., and can solve the large demand that cannot meet the industrial development and production of such products, and the extraction time is long , large amount of solvent, etc., to achieve the effect of protecting myoblasts from oxidative damage, shortening the extraction time, and reducing the amount of reagents

Active Publication Date: 2020-09-25
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hot water extraction is the most commonly used method for extracting polysaccharides. The process is simple, but it has disadvantages such as long extraction time, large amount of solvent used, and high temperature is easy to destroy the structure of polysaccharides; enzymatic hydrolysis is highly specific and can only enzymatically hydrolyze specific glycosidic bonds , the cost of enzyme preparation is relatively high (Yang Yong, Shou Kunxiu, Li Lilang, et al. Process optimization of Pleurotus ostreatus polysaccharides extracted by compound enzymatic method [J]. Journal of Mountain Agricultural Biology, 2019,38(1):29-34); Ultrasound Auxiliary extraction is noisy (Deng Bin. Process optimization of Pleurotus ostreatus polysaccharides extracted by ultrasonic combined with enzymatic method [J]. Journal of Heilongjiang Bayi Agricultural Reclamation University, 2019,31(5):66-72); microwave-assisted extraction is limited by equipment, The extraction amount is small (Zhang Xiao, Zhu Caiping, Deng Hong, et al. Uniform design and optimization of ultrasound-assisted enzymatic extraction of Pleurotus ostreatus polysaccharides [J]. Food and Machinery, 2016, 32(9): 166-171)
None of the above-mentioned existing technologies can meet the large demand for industrialized development and production of such products.

Method used

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  • Oyster mushroom galactomannan as well as preparation method and application thereof
  • Oyster mushroom galactomannan as well as preparation method and application thereof
  • Oyster mushroom galactomannan as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Pulverize the dried fruiting bodies of flat mushrooms and pass through a 40-mesh sieve to form dry fruiting body powders of flat mushrooms. The dried fruiting bodies of flat mushrooms are packed into a wall breaking machine, and deionized water is added according to a material-to-liquid ratio of 1:40. The wall was broken for 10 minutes, and a single-screw extruder was used for extrusion and filtration. The diameter of the die hole of the single-screw extruder was 16 mm, the temperature of the sleeve of the extruder was 58° C., and the screw speed was 220 r / min. Concentrate the extract at 70°C to 1 / 3 of its original volume, cool to room temperature, add 3 times the volume of absolute ethanol, let stand at 4°C for 12 hours, centrifuge at 10,000 r / min for 5 minutes, volatilize the residual ethanol, freeze-dry, and the dry powder is flat Mushroom fruiting body crude polysaccharide.

[0027] 2, Pleurotus ostreatus sporocarp crude polysaccharide is prepared into 5mg / ml sol...

Embodiment 2

[0030] 1. Pulverize the dried fruiting bodies of flat mushrooms and pass through a 40-mesh sieve to form dry fruiting body powders of flat mushrooms. Put the dried fruiting bodies powder of flat mushrooms into a wall breaking machine, add deionized water according to the ratio of material to liquid at 1:10, The wall was broken for 20 minutes, and a single-screw extruder was used for extrusion and filtration. The diameter of the die hole of the single-screw extruder was 8mm, the temperature of the extruder sleeve was 90°C, and the screw speed was 240r / min. Concentrate the extract at 90°C to 1 / 4 of its original volume, cool to room temperature, add 4 times the volume of absolute ethanol, let it stand at 4°C for 12 hours, centrifuge at 5000r / min for 10 minutes, volatilize the residual ethanol, freeze-dry, and the dry powder is flat Mushroom fruiting body crude polysaccharide.

[0031]2, Pleurotus ostreatus sporocarp crude polysaccharide is prepared into 5mg / ml solution, adds chlo...

Embodiment 3

[0034] 1. Pulverize the dried fruiting bodies of flat mushrooms and pass through a 40-mesh sieve to form dried fruiting bodies of flat mushrooms. The dried fruiting bodies of flat mushrooms are packed into a wall breaking machine, and deionized water is added according to a material-to-liquid ratio of 1:80. The wall was broken for 2 minutes, and a single-screw extruder was used for extrusion and filtration. The diameter of the die hole of the single-screw extruder was 16 mm, the temperature of the sleeve of the extruder was 40° C., and the screw speed was 160 r / min. Concentrate the extract at 80°C to 1 / 3 of its original volume, cool to room temperature, add 3 times the volume of absolute ethanol, let it stand at 4°C for 12 hours, centrifuge at 7000r / min for 8 minutes, volatilize the residual ethanol, freeze-dry, and the dry powder is flat Mushroom fruiting body crude polysaccharide.

[0035] 2, Pleurotus ostreatus sporocarp crude polysaccharide is prepared into 5mg / ml solution...

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Abstract

The invention relates to oyster mushroom galactomannan as well as a preparation method and an application thereof, and belongs to the field of preparation of effective components of edible mushrooms.The pleurotus ostreatus galactomannan is obtained by performing normal-temperature wall-breaking extraction on dried fruiting bodies of pleurotus ostreatus, and combining extrusion separation, proteinremoval and ion exchange chromatography. According to the wall breaking in combination with extrusion filtration, in the preparation method, the extraction time is shortened, the reagent consumptionis reduced, the energy is saved, and the polysaccharide yield is improved; and an adopted extrusion filtration technology is suitable for separation of a viscous solution, and the obtained oyster mushroom galactomannan has the functional activity of protecting myocyte oxidative damage, and can be used for preparing auxiliary medicines and health foods for protecting myocyte oxidative damage.

Description

technical field [0001] The invention relates to oyster mushroom galactomannoglucan and its preparation method and application, belonging to the field of natural products. Background technique [0002] Pleurotus ostreatus, scientific name Pleurotus ostreatus, ranks among the top three in the annual output of edible fungi in my country. Pleurotus ostreatus is rich in nutrients such as protein, polysaccharides, vitamins, and trace elements. Traditional Chinese medicine believes that it has the effects of treating waist and leg pain, numbness of limbs, and relaxing tendons and activating collaterals. Polysaccharide is the main active ingredient of Pleurotus ostreatus to exert its pharmacological effects. Restricted by the immature development of technology related to the preparation of Pleurotus ostreatus polysaccharides and products such as medicines or health foods, especially the research on the efficacy of Pleurotus ostreatus polysaccharides, such as the hepatoprotective ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/736A61P39/06A23L33/125A23L31/00
CPCC08B37/0087A61K31/736A61P39/06A23L33/125A23L31/00A23V2002/00A23V2200/30A23V2250/51A23V2250/208
Inventor 王琦苏玲刘宇刘岱尧
Owner JILIN AGRICULTURAL UNIV
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