1039 results about "Pleurotus ostreatus" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The invention relates to a strain Pleurotus ostreatus TIB.BPE.10010 with the accession number being CGMCC No.6232. The invention also relates to a method of preparing L-erythrothioneine. The method comprises steps of culturing and fermenting the Pleurotus ostreatus CGMCC No.6232 to perform biosynthesis to form the L-erythrothioneine and extracting the L-erythrothioneine from mycelium cells.

The invention discloses a preparation method for an edible fungus flavor food. The edible fungus can be any one from edible agaricus bisporus, straw mushrooms, oyster mushrooms, shii-take, needle mushrooms, pleurotus nebrodensis, pleurotus eryngii, agrocybe aegerita, hypsizigus marmoreus, agaricus pratensis schaeff, spring mushrooms, stropharia rugoso-annulata, sparassis crispa, macrolepiota procera, tremellodon gelatinosum, black trumpet mushrooms, hericium erinaceus, pholiota nameko, lepista personata, mitake mushrooms, lactarius hatsudake, golden oyster mushrooms, pholiota adiposa, cantharellus cibarius, termitomyces albuminosus, tricholoma giganteum, pleurotus citrinopileatus, calocybe gambosa, truffle, amillariella mellea, fungus suillus, beef-steak fungus, lactarius deliciosus, tricholoma matsutake, agaricus bitorquis, pleurotus pulmonarius, toadstool and russula vinosa, and after treatment, the edible fungus is steeped into a steeping liquor prepared from red wine vinasse, high quality dry red wine and a flavouring agent in a vacuum and normal temperature and normal pressure combined condition, so as to obtain the flavor food; the edible fungus flavor food for leisure is prepared through oil-bath dewatering; a flavor food flavor puffed food is obtained through a swelling process, so as to meet consumers' hobbies and requirements.

The invention relates to an oyster mushroom culture medium and an oyster mushroom culture method using the oyster mushroom culture medium. The oyster mushroom culture medium comprises crab-flavor mushroom fungus chaff, wood scraps, cottonseed hulls, corncobs, bran, quicklime, gypsum and the like. The crab-flavor mushroom fungus chaff, the cottonseed hulls, the corncobs, the wood scraps, the bran and the like are used for preparing the oyster mushroom culture medium, so that environment pollution possibly caused by the materials is solved, various rich nutrients are provided for growth of oyster mushrooms and the yield of the oyster mushrooms in each turn is improved; by the oyster mushroom culture method, the biological efficiency of the oyster mushrooms is 92-98% and the production cost of the oyster mushrooms is reduced; therefore, the oyster mushroom culture medium and the oyster mushroom culture method have very important environment-friendly and economic significance and have a very good application prospect.

The invention relates to an oyster mushroom growing medium by the utilization of herb residue and biogas residue, and a preparation method thereof. The components of the growing medium are: herb residue, biogas residue, lime powder, gesso, urea, calcium superphosphate, and wheat bran. By combining biogas residue with herb residue, and complementing proper auxiliary materials, the invention ensures that the medium nutrient is complementary to the granule size, thus hitting the target of normal bacteria growing, normal mushroom growing, and stable production without the addition of other raw materials. The invention also provides a method for preparing the growing medium. The cost of the oyster mushroom growing medium is 80 percent lower than that of cottonseed shell raw material and around 60 percent lower than that of straw materials such as corn cob. Usually, the biological efficiency is about 120 percent.

The invention discloses an oyster mushroom cultivation material. The cultivation material is characterized by using hickory branch chips, nutshell chips and husk chips as main raw materials, and using mushroom dreg, bran, calcium superphosphate, gypsum powder and the like as auxiliaries, wherein the water content is 63-65%. The invention further discloses a manufacturing method of the cultivation material. The manufacturing method and the cultivation material have the following advantages: 1, the hickory branch chips, the nutshell chips and the husk chips are insolated by sunshine, secondarily fermented or piled up to be aged, so that the toxic bacteriostats are degraded, and the macromolecular substances such as lignin and cellulose are decomposed to simple substances which are beneficial to absorb by oyster mushroom hypha, and the growth of the oyster mushroom hypha can be promoted; 2, the cultivation material proportion is reasonable, the bacterial material is good in breathability, the water-retaining property is strong, the effective mushroom quantity is large, the yield is high, and the biological efficiency is up to 130.3-142.6%; 3, the preparation method provides a solution for innocent treatment of hickory branch, nutshell and husk, and provides a new substrate for cultivating the oyster mushroom.

The invention discloses a super-high-yield oyster mushroom culture method and belongs to the field of edible mushroom production and plantation. According to the method, corn flour, beam pulp, compound fertilizer and the like are added in a culture medium so that yield and quality can be obviously improved; lotus seed hulls are added to play a role of loosening and ventilation, and tide-turning time is shortened. Fruiting is achieved at one end, nutrition is supplied in a concentrated mode, the product quality is high, and the shapes of the mushrooms are good. Nutrient solution containing nitrogen, sugar and various mineral elements, trace elements and vitamin is supplemented to the oyster mushrooms at a later stage. According to the super-high-yield oyster mushroom culture method, an oyster mushroom production and culture formula is changed and combined with the culture mode of later-period nutrient solution supply and one-end fruiting, the oyster mushrooms are stable, high and excellent in yield, production efficiency can be effectively improved, the utilization rate of a land is improved, and the super-high-yield oyster mushroom culture method is suitable for economical and large-scale production.

The invention relates to a vegetable culture technique, in particular to an oyster mushroom culture technique, which belongs to the technical field of crop culture. The oyster mushroom culture technique comprises mixing culture materials, fermenting the culture materials and breeding varieties. The technique ensures high yield, good quality and complete and nice appearance of the oyster mushroom by using cotton seeds, bran, soybean cake meal and gypsum powder as the culture materials through the fermentation of the culture materials and the breeding and the culture of strains, so the technique can satisfy the market demands.

The invention belongs to the field of edible fungus cultivation and discloses a method for three-dimensionally cultivating white mushroom by a wall soil-covered cultivation process. The method comprises the following steps: building a pergola, producing fungus bags, preparing composite nutrient soil, ripping the bags and making a wall, and is characterized in that: white mushroom bags with hyphae are arrayed and vertically piled in the pergola, and are connected by the composite nutrient soil and the prepared nutrient soil so as to form rows of stable mushroom walls; and the method realizes the frugal three-dimensional cultivation of the white mushroom and effectively solves the problem that a cultivation material loses water. The wall soil-covered cultivation process of the invention creates a small climatic ecological environment for the white mushroom, can increase discharge per unit area, improve land utilization rate, achieve the effect of preserving moisture and increase the yield by 30 percent, breaks a new way for high-yield cultivation of the white mushroom without increasing cost and consuming energy, and can be popularized and applied in vast rural areas.

The invention discloses a method for cultivating oyster mushroom with corn straws. The method mainly comprises four steps: treating the corn straws with quick lime; preparing a culture medium; feeding materials and inoculating; and culturing the mushroom and growing the mushroom. In the method, the corn straws are treated by the quick lime so that the lime plays roles of sterilization and bacteriostasis, thus achieving a purpose of controlling growth of mixed bacteria in the corn straws, and solving a problem of failure of oyster mushroom cultivation with a raw material of the corn straws due to the mixed bacteria growth.

The invention relates to a Pleurotus ostreatus cultivation medium. The Pleurotus ostreatus cultivation medium is prepared through using the following raw materials, by weight, 40-50 parts of wheat straw, 20-30 parts of oat straw, 4-6 parts pear leaf powder, 5-6 parts of vinegar residue, 10-15 parts of expired bread, 8-10 parts of expired biscuit, 8-10 parts of bean dregs, 5-6 parts of cattle bone meal, 2-3 parts of quicklime, 0.3-0.4 parts of calcium carbonate, 1-2 parts of quicklime, 0.5-1.0 part of calcium superphosphate and 5-10 parts of a sustained-release nutritional particle. The Pleurotus ostreatus cultivation medium have the advantages of wide sources of the raw materials, increase of the healthcare efficacies of liver nourishing and eyesight improving of Pleurotus ostreatus cultivated through the medium, and low raw material cost.

The invention relates to a method for preventing and controlling mixed fungus through table salt used in oyster mushroom raw material cultivation. The method is characterized in that when leftovers of agricultural and sideline products, such as sawdust, cottonseed hull, corn cob, cotton straw, ramie straw, rape straw, asparagus straw, cassava straw, cassava dregs, cotton boll shell and peanut shell are used for preparing an oyster mushroom cultivation material, a proper amount of table salt is added to kill or inhibit mixed fungus in the cultivation material. The invention further discloses a preparation method of the cultivation material. Compared with the method in which carbendazim pesticide is used, the method provided by the invention has the advantages that: 1. the anti-hybrid ability is strong and the effect is higher than 99%; 2. the method promotes the growth of the oyster mushroom mycelium and shortens the production cycle; and 3. the produced oyster mushroom has high quality and is free of residual harmful substances, and the yield is increased by more than 20%.

The invention relates to the technical field of edible mushroom cultivation, in particular to a method for using pruned mango branches and leaves as raw materials to cultivate oyster mushrooms under mango trees, which specifically includes: building a cultivation bed under each mango tree in a mango forest, using mango branches and leaves as the raw materials to make oyster mushroom logs for timely cultivation in early February and early October each year at proper temperature, not in rainy seasons, planting the oyster mushroom logs on the beds, and after production management, starting mushroom harvesting in late March and late November. By the method, land output rate can be increased, resource utilization rate can be increased and agricultural labor productivity can be improved. Soil conditions can be improved. Fruit tree growth and ecological environment protection are benefited. By the cultivation mode, farmers can implement the three-dimensional agricultural production mode to achieve high yield of both mango fruits and oyster mushrooms while investment is not increased and yield of other crops is not reduced.

The invention discloses mushroom-meat mixed food which is prepared by taking mushrooms as raw materials, and table salt, white sugar, monosodium glutamate and the like as auxiliary materials, wherein the dosage of the auxiliary materials according to mass percentages in the raw materials is as follows: 1.5-2.5% of the table salt, 1.5-2.5% of the white sugar, 0.1-0.2% of the monosodium glutamate, 0.01-0.02% of I + G, 4-6% of fresh meat, 0-0.5% of No.3618 meat powder, 1.6-2.0% of food gum, 0.05-0.15% of compound phosphate, 0.03-0.06% of citric acid, 1.5-2.5% of cooking oil and 0-0.05% of capsicum red pigment. The mushroom-meat mixed food has original mushroom fragrance of oyster mushrooms or pleurotus eryngii, and full-bodied meat fragrance of the fresh meat, and the edible mushrooms have certain formability by adding the food gum, so that the mushroom-meat mixed food is easy to slice and better in chewiness, and the mouthfeel of the mushroom-meat mixed food is also greatly improved.

The invention relates to the technical field of cultivation of edible fungi, in particular to a method for cultivating oyster mushroom by using tomato straws as main raw materials. The method particularly comprises the following steps of preparing culture mediums for oyster mushroom production by using the tomato straws with abundant resources as the main raw materials; fermenting the main raw materials comprising tomato straw crushed substances, miscellaneous wood sawdust, rice bran and the like, directly mixing with the oyster mushroom strains according to the ratio, bagging, and cultivating until the fungus stick for fungus preparation and mushroom production; and realizing three-dimensional cultivation by a fungus stacking wall. By the method, the tomato straws can be transformed and utilized greatly in the tomato producing area, pollution to atmosphere, soil, water and environment caused by incineration is reduced, the industry chain is prolonged, multi-level value increment of agricultural resources is realized, the industry additional value is increased, and the income of farmers is increased.

The invention discloses pickled oyster mushrooms and a preparation method thereof. The pickled oyster mushroom product is prepared by using 600 weight parts of oyster mushrooms, 36 weight parts of table salt, 36 weight parts of white granulated sugar, 1,200 parts of water and leaven as raw materials. The leaven may be old pickling water, Lactobacillus pentosus or Lactobacillus plantarum standard stain, wherein the old pickling water is added according to a weight part ratio of the old pickling water to the oyster mushrooms of 1:10, and the Lactobacillus pentosus or Lactobacillus plantarum standard stain is added till the bacterium content in the solution reaches 10<7> to 10<8> cfu/mL. The preparation method comprises: placing the pretreated oyster mushrooms, water solution of table salt and white granulated sugar and leaven into a pickling jar; sealing the mouth rim of the pickling jar, placing the pickling jar in a constant-temperature culture tank at 20 DEG C, fermenting for 18 days, and obtaining the pickled oyster mushroom product. The invention solves the problem of short oyster mushroom preservation period; and the preparation method is simple, quick and suitable for industrial production.

The invention discloses a microbe combination of degraded stalks and its preparing method as well as a method of degrading the stalks, where the microbe combination includes: liquid bacterial strain, and solid bacterial strain, the liquid bacterial strain is made by culturing plant lactobacillus, Streptococcus faecali, and Candida utilis in the culture medium and the solid bacterial strain is made by culturing Phanerochaete chrysosporium and Pleurotus ostreatus; the method of degrading the stalks by the combination: mixing the liquid bacterial strain with the stalks raw material in weight ratio of 1 to 15000-25000, adding in 0.9% NaCl water solution to make the water ratio reach 65-70%, sealing and fermenting in a sealed container as 25-30deg.C for 7-10 days and take out; adding in the solid bacterial strain and mix uniformly, fermenting at 25-30 deg.C, and when the mycelium grows completely and drying into the finished products. It simulates the ecological mode of biodegrading stalks in the nature, fully uses specific enzyme system of each bacterial strain, and achieves the purpose of biodegrading stalks.

The invention discloses a method for cultivating oyster mushrooms. The method includes the following steps of mother culture making, stock culture making, preparing and applying of standard selenium liquid, cultivating culture making and mushroom producing management. According to the method for cultivating oyster mushrooms, inorganic selenium is biologically converted into organic selenium facilitating absorption of the human body; meanwhile, the content of the selenium of the oyster mushrooms is increased, the food-drug health caring value of the oyster mushrooms for people is increased, the yield is increased by about 30% on the basis of the original yield, the yield is increased, the enrichment amount of the selenium in every 100 g of fresh mushroom bodies can be 0.1-0.2 g, and the quality of the oyster mushrooms is improved.

The invention discloses a culture medium for edible fungi. The prepared culture medium for edible fungi is prepared from the following raw materials in percentage by mass: 65.34-81.54% of salix mongolica wood flour, 4.02-19.89% of bran, 3.82-10.49% of bean pulp, 3.72-7.15% of corn flour and 2.0% of plaster stone, wherein the sum of the mass percents of the raw materials is 100%. The culture medium for edible fungi is suitable for cultivation of multiple edible fungi such as Pleurotus eryngii, Flammulina velutipes, Pleurotus citrinopileatus, Pleurotus ostreatus, Pleurotus nebrodensis, Lentinus edodes, Exidia auricuia and the like.

The method of cultivating edible fungi by using the discarded branches and leaves of urban greening, at least one kind of urban greening branches and leaves among ginkgo, zelkova, magnolia or white birch is preliminarily sorted, washed, dried, crushed, mixed with nutrients and water, and stirred evenly , to make edible fungus culture material, put the edible fungus culture material into bags and carry out high-temperature sterilization. Flammulina velutipes common edible fungus. The invention uses the discarded branches and leaves of landscaping as raw materials, which not only effectively solves the problem of shortage of raw materials for cultivating edible fungi, but also reasonably solves the problem of waste disposal in current landscaping. The edible fungi cultivated by the invention are rich in nutrition, good in mushroom quality and high in yield ,with broadly application foreground.

The invention relates to an oyster mushroom culture medium as well as a fermentation inoculum and application thereof, belonging to the technical field of edible mushroom culture. The fermentation inoculum of the oyster mushroom culture medium is obtained through the following steps of: preparing first-level strain liquids by respectively culturing Actinomycete strains W-4 and W-10 and bacterium strains N-14 in liquid culture mediums with same volumes under the conditions of respectively appropriate fermentation temperature for same time; mixing the first-level strain liquids into a mixed bacteria solution according to the volume ratio of 3:3:1; and carrying out second-level amplification culture at 30-35 DEG C for 60 hours. The invention also provides an oyster mushroom culture medium prepared through the fermentation of the fermentation inoculum. The oyster mushroom culture medium has the biological conversion rate of 120-130 percent in oyster mushroom culture; and in addition, compared with a common culture medium, the yield of the oyster mushroom culture medium is increased by 20-30 percent.