Microorganism compositions for degrading straw, preparation thereof and method for degradation of straw

A technology of microbial composition and straw is applied in the field of degrading crop waste to achieve the effects of increasing economic benefits, reducing costs and saving energy

Inactive Publication Date: 2004-11-10
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on the microbial degradation of straw and the development o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of liquid strains:

[0042] (1) Medium preparation: 100ml tomato juice, 5g yeast extract, 5g peptone, 10g sugar, KH 2 PO 4 Add 2g of water to 1000ml to make a liquid medium;

[0043] (2) Strain preparation: Lactobacillus plantarum, Streptococcus faecalis, and Candida utilis were inoculated in 1000ml liquid medium, cultivated at 30°C for 48 hours, and the total number of bacteria per milliliter reached 10 9 The above promptly becomes liquid spawn.

[0044] 2. Preparation of solid bacteria:

[0045] (1) Culture medium preparation: add 78g of cornmeal or 20g of wheat bran to straw powder, add KH 2 PO 4 2.0g MnSO 4 0.2gMgSO 4 ·7H 2 O 1.6g Vitamin B 1 10 -3 g CaCl 2 Add 0.05g of water to make the water content reach 65-70% to make a solid medium;

[0046] (2) Strain preparation: inoculate the white rot fungus and Pleurotus pleurotus in a solid medium, and cultivate at 25-27° C. until the hyphae are overgrown to become a solid strain.

Embodiment 2

[0048] 1. Preparation of liquid strains:

[0049] (1) Medium preparation: 100ml tomato juice, 5g yeast extract, 5g peptone, 10g sugar, KH 2 PO 4 Add 2g of water to 1000ml to make a liquid medium;

[0050] (2) Strain preparation: Lactobacillus plantarum, Streptococcus faecalis, and Candida utilis were inoculated in 1000ml liquid medium, cultivated at 30°C for 48 hours, and the total number of bacteria per milliliter reached 10 9 The above promptly becomes liquid spawn.

[0051] 2. Preparation of solid bacteria:

[0052] (1) Culture medium preparation: add 78g of cornmeal or 20g of wheat bran to straw powder, add KH 2 PO 4 2.0g MnSO 4 0.2gMgSO 4 ·7H 2 O 1.6g Vitamin B 1 10 -3 g CaCl 2 Add 0.05g of water to make the water content reach 65-70% to make a solid medium;

[0053] (2) Strain preparation: inoculate the white rot fungus and Pleurotus pleurotus in a solid medium, and cultivate at 25-27° C. until the hyphae are overgrown to become a solid strain.

Embodiment 3

[0055] 1. Preparation of liquid strains:

[0056] (1) Culture medium preparation: mix tomato juice 80ml, yeast extract 3g, peptone 8g, sugar 15g, KH 2 PO 4 Add 1g of water to 1000ml to make a liquid medium;

[0057] (2) Strain preparation: Lactobacillus plantarum, Streptococcus faecalis, and Candida utilis were inoculated in 1000ml liquid medium, cultivated at 30°C for 48 hours, and the total number of bacteria per milliliter reached 10 9 The above promptly becomes liquid spawn.

[0058] 2. Preparation of solid bacteria:

[0059] (1) Culture medium preparation: add KH to 71g of cornmeal or 15g of wheat bran 2 PO 4 1.0g MnSO 4 0.1gMgSO 4 ·7H 2 O 1.1g Vitamin B 1 10 -4 g CaCl 2 Add 0.01 g of water to make the water content reach 65-70% to make a solid medium;

[0060] (2) Strain preparation: inoculate the white rot fungus and Pleurotus pleurotus in a solid medium, and cultivate at 25-27° C. until the hyphae are overgrown to become a solid strain.

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PUM

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Abstract

The invention discloses a microbe combination of degraded stalks and its preparing method as well as a method of degrading the stalks, where the microbe combination includes: liquid bacterial strain, and solid bacterial strain, the liquid bacterial strain is made by culturing plant lactobacillus, Streptococcus faecali, and Candida utilis in the culture medium and the solid bacterial strain is made by culturing Phanerochaete chrysosporium and Pleurotus ostreatus; the method of degrading the stalks by the combination: mixing the liquid bacterial strain with the stalks raw material in weight ratio of 1 to 15000-25000, adding in 0.9% NaCl water solution to make the water ratio reach 65-70%, sealing and fermenting in a sealed container as 25-30deg.C for 7-10 days and take out; adding in the solid bacterial strain and mix uniformly, fermenting at 25-30 deg.C, and when the mycelium grows completely and drying into the finished products. It simulates the ecological mode of biodegrading stalks in the nature, fully uses specific enzyme system of each bacterial strain, and achieves the purpose of biodegrading stalks.

Description

technical field [0001] The invention relates to a microbial composition, a preparation method thereof and a method for degrading crop waste. Background technique [0002] Straw cellulose is the most abundant potential feed resource on the earth. It has the characteristics of large quantity and low price. If it can be degraded into animal feed resources, it is especially important for developing countries with more people and less land. [0003] The main components of crop straw are cellulose, hemicellulose and lignin, which account for about 80% of the dry weight of straw. Among them, cellulose accounts for about 30-35%. It is a polymer compound formed by condensing glucose molecules through β-1,4 glucosidic bonds, including 2000-10000 glucose units. Natural cellulose exists in a linear chain structure, and there is a certain crystalline structure and an amorphous structure with a poor arrangement order between the chains. Many cellulose mole...

Claims

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Application Information

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IPC IPC(8): B09B3/00C12N1/00C12P39/00C12S3/04
Inventor 陈秀为赵华
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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