15 results about "Phanerochaete" patented technology

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The application discloses a kind of for decomposing forest litter composite microbial inoculant, preparation method and its application method, it is related to litter decomposition technical field.The application includes a kind of for decomposing forest litter composite microbial inoculant preparation method, comprising the following steps: S1.to Phanerochaete chrysosporium, Trichoderma reesei and ropegillus catenulatum are respectively carried out directional metabolism preactivation;S2.the three preactivation wet bacteria bodies obtained in step S1 are mixed according to the fresh weight ratio of Phanerochaete chrysosporium:Trichoderma reesei:ropegillus catenulatum 3:2:1, obtain mixed bacteria body;S3.mixed bacteria body is mixed with the straw carrier that is preprocessed by steam explosion according to the mass ratio of bacteria body fresh weight:carrier dry weight 1:2, under the condition of temperature 28 DEG C, relative humidity 92~95%, stationary culture 72 hours, carry out phased micro-perturbation aeration, construct physical symbiosis;S4.the material obtained in step S3 is dried to 15% moisture content at 40 DEG C, obtain composite microbial inoculant;Efficient and stable decomposition of pine forest litter is realized.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The invention discloses a siderophore-mediated symbiotic degradation bacterial agent for remediation of petroleum hydrocarbon contaminated soil. The microbial agent is formed by compounding a high-yield siderophore bacterium pseudomonas aeruginosa DG-1 with the strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No.28382 and a fungus white rot fungus BNCC336258 according to the volume ratio of 1: (2 to 4), and through the siderophore-mediated bacterium-fungus synergistic metabolism effect, the remediation efficiency of the petroleum hydrocarbon contaminated soil under the iron limiting condition (the free iron concentration is smaller than or equal to 1.0 * 10 <-18 > M) is improved. The microbial agent is suitable for treating low-concentration polluted soil with the petroleum hydrocarbon concentration of 0.5-5 wt%, and the total petroleum hydrocarbon removal rate after remediation can reach 80% or above.

The invention discloses a culture method for inducing mycelia to secrete specific extracellular polymeric substances and application of the culture method in sand stabilization, and relates to the field of biotechnology and environmental materials.The culture method comprises the following steps that phanerochaete chrysosporium is inoculated into a liquid fermentation culture medium, and fermentation is conducted under the conditions that the pH ranges from 4.5 to 5.5, the temperature ranges from 35 DEG C to 37 DEG C and shaking culture is conducted; the liquid fermentation culture medium contains tween-80 with the concentration of 0.5-4 g / L and hydrogen peroxide with the concentration of 0.05-0.15 mM as an inducer combination; after the fermentation is finished, separating to obtain fermentation supernate, namely the functional extracellular polymeric substance fermentation liquor; according to the culture method for inducing the mycelium to secrete the specific extracellular polymeric substance and the application of the culture method in sand stabilization, the functional EPS is obtained from the source of regulating and controlling microbial metabolism, and the limitation that a traditional mycelium material only depends on the physical form is broken through.

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for wound healing, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and composite biological bacteria, and carrying out enzymolysis to obtain an enzymolysis product; the composite biological bacterium is a mixture of phanerochaete chrysosporium and streptomyces griseus; carrying out gradient alcohol extraction on the enzymolysis product, merging alcohol extracts, extracting with petroleum ether, and concentrating an organic phase to obtain a component 1; the component 1 is adsorbed by macroporous adsorption resin, the resin is washed with deionized water and then eluted with an eluent, 5-35 min eluent is collected, combined and dried, and the component 1 is obtained. The elution phase is a mixture of cyclohexane, ethyl acetate and acetonitrile. The loropetalum chinense extract is prepared through a specific preparation method, and the wound healing effect is remarkably improved.

This invention provides a solid-state fermentation inoculant, its preparation method, and its application. The preparation method of the solid-state fermentation inoculant involves inoculating *Phanerochaete chrysosporium* into a seed carrier and culturing it in a sterile, pure oxygen environment. Under pure oxygen conditions, the inoculated *Phanerochaete chrysosporium* exhibits a significantly increased reproduction rate, forming a high-density microbial community. Simultaneously, the continuous and stable nutrient supply from the seed carrier enables the inoculant to quickly establish a competitive advantage upon entering materials containing other microorganisms. By seizing nutrients and oxygen resources, it inhibits the growth and reproduction of other microorganisms, ultimately increasing the abundance of white-rot fungi in the fermented product, thereby enhancing the degradation efficiency of petroleum hydrocarbons in the soil. Compared to traditional solid-state fermentation methods, the inoculant production process is very simple and low-cost. The solid-state fermentation inoculant product of this invention has a high microbial density and a relatively large number of target strains, resulting in higher efficiency in soil pollution remediation and increased soil fertility.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant, a preparation method and application. The preparation method comprises the following steps: selecting an anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and a phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776, inoculating the anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and the phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776 into a carrier, and mixing and compounding to obtain the composite microbial inoculant, so that the composite microbial inoculant has good air permeability and flexibility due to the pore structure of the carrier buckwheat hull; the mass transfer efficiency and the adsorption effect of oxygen molecules can be improved through synergistic interaction; when the complex microbial inoculant is applied to degradation of compost and agricultural and forestry waste, the enzyme activity and high temperature resistance of the compost and the agricultural and forestry waste can be effectively improved, synergistic complementation on degradation of lignin, cellulose and hemicellulose is achieved, and the degradation efficiency of the lignin, the cellulose and the hemicellulose is high. In the mixing application of vinasse and straw, the temperature can be rapidly increased, and the temperature can be kept at 60-80 DEG C or above for 2 weeks.

The present application belongs to the technical field of waste conversion fertilizer, and provides a kind of compound organic fertilizer and its preparation method, the method steps are as follows: S1, by weight, rice straw 27-30 parts, bran 9-10 parts and animal manure 8-9 parts are mixed to obtain initial raw material;S2, 0.85-0.9 parts of oxidized modified straw biochar and 0.5-0.55 parts of ammoniated superphosphate are added into the initial raw material at the same time and mixed, and then the moisture content is adjusted to 53±3%, to obtain the base material;S3, add phanerochaete chrysosporium suspension and bacillus subtilis activation solution to the base material and mix evenly to obtain fermentation material;S4, the fermentation material is composted and fermented for more than 28 days to obtain the compound organic fertilizer.The present application shortens the composting time to improve the preparation efficiency of organic fertilizer, and further reduces the phytotoxicity of the prepared organic fertilizer.

The invention discloses a method for preparing a humic acid fertilizer synergist based on biological-chemical activated weathered coal, and relates to the technical field of humic acid fertilizer synergists. The method comprises the following steps: mixing bacillus subtilis, trichoderma harzianum and phanerochaete chrysosporium stock seeds to obtain a complex microbial inoculant, and activating the weathered coal by using the complex microbial inoculant to obtain biologically activated weathered coal; mixing the biologically activated weathered coal with potassium hydroxide and paper pulp lignin liquid, and performing chemical solid-phase activation under a heating condition to obtain an activated product, namely the humic acid fertilizer synergist. Through synergism of biological activation and chemical activation, the content of humic acid, organic matters and fulvic acid in the humic acid fertilizer synergist can be obviously increased, so that growth and development of rape are facilitated. Moreover, the weathered coal is activated by adopting the paper pulp lignin liquid, and meanwhile, the resource utilization of the paper pulp lignin liquid and the high-order conversion of the weathered coal are realized, so that the method has great practical significance.

The application relates to the technical field of agricultural solid waste resource utilization, and discloses a composite microbial inoculum for fertilizer preparation of multi-source agricultural and forestry organic waste and a preparation method thereof, which comprises the following steps: the composite microbial inoculum is prepared from D-anhydrous glucose, an induced mesoporous carrier dispersion liquid, veratryl alcohol, urea, a phanerochaete chrysosporium seed liquid, a trichoderma reesei seed liquid and a bacillus licheniformis seed liquid. Two-stage time sequence fermentation is adopted during preparation, fungi are inoculated and cultured first, then bacteria are inoculated and urea is added. Ammonia produced by bacterial catalysis is used to drive the spontaneous increase of the pH of a fermentation system, trigger the in-situ crystallization of precursor ions, and build a multi-level layered coating configuration with a porous diatomite skeleton in the inside, an alkali lignin planting layer in the middle layer, and an ammonium magnesium phosphate crystal armor layer in the outer layer. The application effectively blocks interspecific competition during co-culture, endows the microbial inoculum and degradation enzyme system with excellent mechanical shear resistance, can provide long-acting acid-alkali buffering and precise nutrient release in the complex environment of composting, and improves the waste degradation efficiency.

The invention relates to the technical field of environmental microorganisms, and discloses a method for directionally synthesizing high-activity humus based on a microorganism co-culture system.The method comprises the steps that firstly, a co-culture flora composed of phanerochaete chrysosporium, bacillus subtilis and agrobacterium tumefaciens according to a specific proportion is constructed; and then inoculating into the pretreated agricultural wastes, and carrying out two-stage temperature-controlled fermentation: in the first stage, carrying out low-temperature aerobic promotion on hydrolysis to produce acid, and in the second stage, carrying out heating micro-aerobic drive on oxidative polymerization. Through the orientation process, the lignocellulose raw material can be efficiently converted into high-activity humus within 12 days. Through the synergistic effect of functional flora and accurate control of physical conditions, directional rapid synthesis of humus is achieved, the total content of humus in the obtained product is high (> = 25%), the ratio of humus to humus is appropriate (1.5-2.0), the biological activity is high (the seed germination index is > = 120%) and is far better than that of a traditional compost product, and a new technology is provided for high-value utilization of agricultural waste and soil improvement.

The invention belongs to the technical field of agricultural planting, and particularly relates to a straw returning method capable of achieving greenhouse gas emission reduction. According to the specific technical scheme, the straw decomposing microbial agent comprises microbial preparations, and the microbial preparations comprise bacillus subtilis, trichoderma harzianum and phanerochaete chrysosporium. When the straw decomposing microbial inoculum is used for composting, the temperature can be quickly increased, the composting efficiency is improved, lignin in the straw can be degraded to 60% or above after composting is carried out for 20 days, and harmful microorganisms and weed seeds in the straw can be effectively killed. By using the microbial agent disclosed by the invention, short-term and in-situ rapid composting can be realized; and the compost can be quickly returned to the field in situ. The yield of crops is guaranteed, and meanwhile the emission amount of greenhouse gas is effectively reduced.

This invention discloses a siderophore-mediated symbiotic degradation agent for the remediation of petroleum hydrocarbon-contaminated soil. This agent is composed of *Pseudomonas aeruginosa*, a high-side-producing bacterium. Pseudomonas aeruginosa DG-1, strain preservation number CGMCC No. 28382) and the fungus white rot fungus ( Phanerochaete chrysosporium BNCC336258 is formulated in a volume ratio of 1:(2-4) and enhances iron-limiting conditions (free iron concentration ≤1.0×10⁻⁶) through siderophore-mediated bacterial-fungal synergistic metabolism. ‑18 The remediation efficiency of this microbial agent on petroleum hydrocarbon-contaminated soil (M) was demonstrated. This agent is suitable for treating low-concentration contaminated soil with petroleum hydrocarbon concentrations of 0.5-5 wt%, achieving a total petroleum hydrocarbon removal rate of over 80% after remediation.