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Method for promoting complex enzyme to catalyze degradation of straws by using active mediator combination

A technology of catalytic degradation and rice straw, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of small environmental pollution, large amount of acid and alkali, high energy consumption, etc., and achieve the purpose of improving the decay rate and operating costs Low, easy-to-operate effect

Inactive Publication Date: 2012-07-11
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many researches mainly use physical and chemical means to treat rice straw, including microwave radiation method and acid and alkali treatment method, etc., but this kind of method consumes a lot of energy, consumes a lot of acid and alkali, and has low recovery, which is easy to cause environmental pollution.
The other is the use of biotechnology to treat rice straw, the main focus of which is to use lignin degrading enzymes (such as lignin peroxidase (LiP), manganese peroxidase (MnP)) to catalyze the degradation of lignocellulose in rice straw , this type of method has less environmental pollution and lower energy consumption, but since it is difficult for polymer substances such as LiP and MnP to enter the intact and undamaged rice straw, the simple use of lignin-degrading enzymes to catalyze the degradation of rice straw low rate

Method used

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  • Method for promoting complex enzyme to catalyze degradation of straws by using active mediator combination
  • Method for promoting complex enzyme to catalyze degradation of straws by using active mediator combination
  • Method for promoting complex enzyme to catalyze degradation of straws by using active mediator combination

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Embodiment 1

[0023] A kind of method that utilizes active mediator combination of the present invention to promote composite enzyme to catalyze and degrade rice straw, comprises the following steps:

[0024] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 60-mesh sieve for later use;

[0025] (2) Preparation of compound enzyme solution: add 2 mL of spores to 200 mL of sterilized liquid medium, the concentration is 1.5×10 6 Phanerochaete chrysosporium spore suspension per mL, cultured with shaking at 30°C and 120 r / min for 6 days, and obtained compound enzyme solution (15 U / L LiP and 213 U / L MnP); the formula of liquid medium (1L) is: glucose 10 g, ammonium tartrate 0.2 g, MgSO 4 0.71 g, Tween 80 0.5 mL, KH 2 PO 4 2.56 g, VB 1 0.01 g, sodium tartrate buffer 4.616 g, trace element solution 100 mL, benzyl alcohol 0.516 mL, H 2 O 899.484 mL, pH adjusted to 4.5;

[0026] (3) Catalytic degradation: Add the compound enzyme solution obtained in step (2) to ...

Embodiment 2

[0034] A kind of method that utilizes active mediator combination of the present invention to promote composite enzyme to catalyze and degrade rice straw, comprises the following steps:

[0035] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 100-mesh sieve for later use;

[0036](2) Preparation of compound enzyme solution: Add 4 mL of spores to 200 mL of sterilized liquid medium (the formula of the liquid medium is the same as that in Example 1) with a concentration of 2×10 6 Phanerochaete chrysosporium spore suspension per mL, cultured with shaking at 37°C and 150r / min for 8 days, and obtained the compound enzyme solution (20 U / L by precipitation, filter paper filtration, high-speed centrifugation and 0.45mm filter membrane filtration) LiP and 250 U / L of MnP);

[0037] (3) Catalytic degradation: Add the compound enzyme solution obtained in step (2) to 5 kg of straw powder obtained in step (1), and at the same time add veratrol solution with a...

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Abstract

The invention discloses a method for promoting complex enzyme to catalyze degradation of straws by using active mediator combination, which includes the following steps: (1) preprocessing the straws: smashing the straws to obtain straw powder and sieving to be reserved; (2) preparing complex enzyme liquid: cultivating phanerochaete chrysosporium in liquid state to obtain the complex enzyme liquid; and (3) catalyzing degradation: adding the complex enzyme liquid into the straw powder, adding active mediator combination simultaneously, keeping warm, preserving moisture, and standing to finish degradation catalyzing. The method for degrading the straws improves enzymolysis rate of lignin in the straws, is favorable for decomposing and transforming and resource utilization of the straws, and is low in cost, simple in operation, low in operation cost, clean and free of pollution.

Description

technical field [0001] The invention relates to the technical field of biodegradation, in particular to a method for promoting composite enzymes to catalyze the degradation of rice straw by combining active mediators. Background technique [0002] Rice straw is an abundant renewable resource and one of the main crop straws. However, because the output of straw varies with the seasons, and the output is large, the value is low, the volume is large, and it is inconvenient to transport. The natural degradation process of straw is extremely slow, resulting in a large amount of straw being dumped directly into the environment in the form of accumulation and burning. The amount of straw is about 5×10 7 t~7×10 7 t, accounting for 10% to 15% of the total straw production, causing great environmental pollution and serious waste of material resources. [0003] Straw is composed of organic matter, a small amount of minerals and water. The organic matter is mainly lignin, cellulose a...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 赵美花曾茁桐曾光明黄丹莲赖萃危臻黄超李宁杰许飘张辰
Owner HUNAN UNIV
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