235 results about "Phanerochaete chryosporium" patented technology

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses a magnetic biological sorbent. The sorbent uses calcium alginate as a carrier, and is embedded with phanerochaete chrysosporium powder and magnetic nanometer particles, wherein, the mass ratio of the phanerochaete chrysosporium powder to the magnetic nanometer particles to the calcium alginate is 1 to (0.5-1.25) to (8-10). The invention also discloses a method for preparing the magnetic biological sorbent. The method comprises the following steps of: adding a mixture of the magnetic nanometer particles and the phanerochaete chrysosporium powder into a 1 to 2 mass concentration calcium alginate solution with 1 to 2 mass concentration, the dosage of the mixture in the solution being between 0.125 and 0.225g / mL, stirring evenly, and obtaining the magnetic biological sorbent through an immobilization process. The magnetic biological sorbent has the advantages of high adsorption efficiency, good selectivity, low cost, simple preparation process and treatment process and so on, and can be effectively applied to the treatment of lead metal in industrial wastewater.

The invention discloses a preparation method for a sludge aerobic composting composite inoculums. According to the method, the Bacillus subtilis (No. 10066), the Bacillus licheniformis (No. 10103), the Lactobacillus acidophilus (No. 6075), the Candida utilis (No. 31272), and the Phanerochaete chrysosporium (No. 40719) that are preserved in China Center of Industrial Culture Collection (CICC) are subjected to individual culture, and are then mixed in proportion and inoculated into a fermentation culture to perform expanded culture, thus obtaining the composite inoculum. At the initial stage of inoculating the composite inoculums to sludge aerobic composting, the time for the compost body to reach a high temperature period is advanced by 4-6 days, the compositing period is shortened by 11-14 days, the stack moisture content is decreased by 18%-25%, and the total nitrogen content of the compost body is increased by 15%-20%, thus improving the composting effect.

The invention discloses a complex microbial agent for composting fermentation and application thereof. The complex microbial agent is a solid inoculant which comprises phanerochaete chrysosporium, streptomyces griseorubens, bacillus subtilis, bacillus methylotrophicus and bacillus amyloliquefaciens. The complex microbial agent is a complex microbial system comprising various microorganisms which have complementary and synergistic functions; the microbial system integrates quick fermentation heat production, biological deodorization and compost material decomposition and degradation and has the effects of a biological fertilizer; the livestock manure compost can be quickly heated to kill off harmful organisms at high temperature; the unpleasant smell is obviously reduced to solve the problem of environment pollution caused by poultry excrement; the microorganisms can be quickly propagated in the poultry excrement fermentation process; the organic matters such as lignocellulose in the excrement can be quickly and effectively decomposed so as to increase the decomposition speed of the compost material and improve the quality of the compost product; the complex microbial agent can be used for producing the biological organic fertilizer so as to reduce the agricultural production cost.

The invention discloses an immobilized biological adsorbent containing phanerochete chrysosporium, the surface of which is uniformly wrapped by a layer of carrier Fe3O4 nanoparticles for fixation. The method comprises the steps of: first adding Fe3O4 nanoparticles into a phanerochete chrysosporium liquid medium containing tween 80, then inoculating a phanerochete chrysosporium spore suspension, conducting shaking cultivation so as to obtain the immobilized biological adsorbent. The invention also discloses application of the adsorbent in dye wastewater treatment. The biological adsorbent of the invention has high adsorption efficiency and large adsorption capacity, and can be effectively used for treating neutral red and lead in dye wastewater. The preparation technology and treatment technology in the invention are characterized by simplicity and low cost.

The invention relates to a method for pre-processing corn stalks through a physical-chemical-biological method. The corn stalks are firstly smashed and physically processed, then the corn stalks are chemically processed through dilute alkali, and finally, the corn stalks are biologically processed through phanerochaete chrysosporium and cellulose degradation bacteria. Lignin removal is facilitated due to dilute alkali processing, the corn stalks can be fully degraded easily because of mixed fermentation, the utilizing rate is increased, and microorganisms producing ethyl alcohol are inoculated into the pre-processed stalks to produce ethyl alcohol, or feeding yeast is inoculated to produce protein feed. The method is simple in technology and convenient to implement.

The present invention relates to a modified straw material, its production method and application. The production method of the modified straw material includes the following steps: 1. steam-blastingstraw to obtain steam-blasted straw; 2. inoculating the steam-blasted straw with culture liquor to make solid fermentation treatment to degrade lignin; 3. mixing the steam-blasted straw and air-driedstraw, making their mixture undergo the processes of thermosetting treatment and forming treatment so as to obtain the invented modified straw material which can be made into plate material or light packaging material instead of wood material, so that it possesses extensive application.

The invention relates to a straw decomposing inoculant which comprises the following components of 0.2-1.5 billion / g of bacillus subtilis, 0.2-1.5 billion / g of bacillus licheniformis, 20-150 million / g of thermophilic sporotrichosis, 20-150 million / g of Trichoderma Koningii and 20-150 million / g of Phanerochete chrysosporium. Therefore, the straw decomposing inoculant has the advantages of low cost and short decomposing time and is practical and easy to operate.

The invention relates to a composite microbial agent used for degradation of garden waste. The composite microbial agent is composed of composite bacterial liquid and a carrier, wherein the composite bacterial liquid comprises Bacillus subtilis, Bacillus licheniformis, Bacillus siamensis, Bacillus tequilensis and Phanerochaete chrysosporium. The bacterial strains of the composite microbial agent produce synergism, so the composite microbial agent can substantially degrade a variety of macro-molecular components in the garden waste, improve the decomposition degree of the waste, enhance the nutrient structure and microfloras of compost and reduce heavy metal pollution. The invention also relates to a preparation method for the composite microbial agent, a method for degrading and converting the garden waste by using the composite microbial agent and fertilizer prepared from products obtained after degradation and conversion of the garden waste with the composite microbial agent.

The invention discloses a grass suppression straw biomass-base degradable mulching film and application. A method for preparing the grass suppression straw biomass-base degradable mulching film includes S1, biologically pretreating straw, to be more specific, compounding phanerochaete chrysosporium and streptomyces rochei, then inoculating the phanerochaete chrysosporium and the streptomyces rochei on the straw, adjusting carbon-nitrogen ratios and moisture contents of material systems and carrying out aerobic fermentation; S2, softening, disassembling and separating straw fibers, to be ore specific, softening the pretreated straw fibers and disassembling the pretreated straw fibers until the fiber lengths are 0.5-2 cm; S3, forming straw biomass-base mulching films, to be more specific, uniformly mixing the straw fibers, wood pulp, methyl celluloses, sodium alginate, gelatin, polyamide epichlorohydrin resin, alkyl ketene dimmers, polyacrylamide, glycerol, alkenyl succinic anhydride grafted starch adhesive and biochar with one another, and carrying out vacuum dehydration, squeezing, drying and forming to obtain the grass suppression straw biomass-base degradable mulching film. The grass suppression straw biomass-base degradable mulching film can be applied to the field of paddy rice seedling raising.

The invention discloses a corn straw fermentation microorganism agent which consists of phanerochaete chrysosporium, trichoderma koningii, bacillus subtilis, bacillus licheniformis and monilia tropicalis. The corn straw fermentation microorganism agent disclosed by the invention has the beneficial effects that the microorganism agent is reasonable in formula and convenient to use; the straw rotting speed is high; the corn straw fermentation microorganism agent has the characteristics of new combination, stable structure, high enzyme activity, high microbial content and the like. The corn straw fermentation microorganism agent can be used for performing high-efficiency and environment-friendly fermentation on corn straw materials, so that a feed can contain a large amount of microorganism live cells, clastic enzymes and other active components, and the utilization rate of the corn straw materials can be effectively increased. The practical significance of solving the problem of environment pollution is achieved; furthermore, the actual application values such as the protein content and the nutritional value in the corn straw materials can be improved; good preconditions are created for recycling application of corn straws.

The invention discloses a method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement. Contaminated soil or oil sludge is oxidized first with a sodium persulfate compound as the oxidizing agent in the presence of a surfactant, and then a mixed flora of bacillus sp. and acremonium sp. Y0997 or phanerochaete sp. F0996 is added twice continuously, so that intensified combined treatment of soil or oil sludge is achieved through intensified combination of chemical oxidization and fungus-bacterium flora biological sequences. In this way, the degrading efficiency of soil or oil sludge severely contaminated by petroleum can be improved remarkably, and repair period can be shortened greatly.

This invention discloses a method for producing fuel ethanol from a straw fibre raw material, comprising: (1) raw material selection; (2) disintegration; (3) pre-processing; (4) double-enzyme saccharifying; (5) sterilization; (6) enlargement culture and application of Pachysolen tannophilus P-01; (7) fermentation; (8) distillation; characterizing: the pre-processing employs white-rot fungi solid culture to decompose lignin. The procedures comprise: (1) bacterium: Coridus versicolor; (2) crude laccase production: (1) culture media producing condition,(2) fermentation: inoculate 6 parti-color Coriolus versicolor seed stopper per 1000ml, 28-30Deg C, 150rpm shake culture for 7d, remove the hypha by filtration so as to obtain the crude laccase; (3) crude peroxidase production: i. culture media, ii. fermentation: inoculate 6 rings of Phanerochete chrysosporium spores per 100ml, 37-39Deg C, static culture for 9d, remove hypha by filtration so as to obtain crude peroxidase; (4) conditions of enzyme liquid decomposing straw: add 1ml crude peroxidase and crude laccase respectively to the straw powder and mix homogenously, and then decompose the straw at 45Deg C, pH 4.5 for 72h. This invention can improve the ethanol yield and decease cost.

The invention relates to a spinning straw fiber extraction method. The spinning rice and wheat straw fiber extraction method is characterized in that the method includes the following steps: 1). the steam explosion pretreatment: straws are air-dried and then are cut into segments; 2). the pretreated straws are put in a steam blasting tank; 3). nutrient glucose and urea are recharged; 4). the strain degumming: first, choosing strains: choosing mushroom white rot fungi or planerochaete chrysosporium as the strain; second, to pave the steam explosion straw mixture obtained in the third step to be 10-100mm high; third, poking the strains onto the straw piles; fourth, culturing on the culturing bed; fifth, cleaning; sixth, bleaching; 5). irradiation curing: the bleached straw fiber is irradiated with Gamma rays with the irradiation amount of 2-10kgy to obtain the radiation curing straw fiber; 6). the straw fiber is oiled; 7) the straw fiber is dried; 8) the dried straw fiber is dispersed to obtain the spinning rice and wheat straw fiber. The invention has the characteristics of environmental protection and low cost.

The invention provides a compound bacterium agent for treating green wastes of gardens as well as a preparation method and application of the compound bacterium agent. The compound bacterium agent isprepared from phanerochaete chrysosporium, trichoderma viride, geotrichum candidum, candida utilis, saccharomyces cerevisiae, bacillus mucilaginosus, bacillus subtilis, lactobacillus plantarum, streptococcus thermophiles and azotobacter chroococcum. When the compound bacterium agent provided by the invention is used for treating the green wastes of the gardens, a carbon-nitrogen ratio and a carbon-phosphorus ratio are adjusted, without the need of adding any auxiliary material; a whole compost fermentation process can be finished through only independently adding the compound bacterium agent,so that the treatment cost of the green wastes of the gardens is effectively saved and a treatment technology is simplified. Microorganisms in the compound bacterium agent can be rapidly and greatly reproduced in a fermentation process, and rapid temperature raising and ageing of compost are promoted; the treatment time of the green wastes of the gardens is shortened and the compound bacterium agent is suitable for treating the green wastes of the gardens in practice.

The invention relates to a pretreatment fungicide for xylose residue or furfural residue, a preparation method and an application thereof, belonging to the technical field of agricultural microbiology. The invention relates to a microbial composite fungicide specially used for promoting the xylose residue or the furfural residue to degrade and improving the production efficiency of methane. The invention firstly adopts liquid fermentation technology to purely breed and ferment all components of the microbial fungicide, and then the components are compounded. The preparation method of the pretreatment fungicide comprises: 0.20-0.40 parts by weight of trichoderma viride liquid, 0.10-0.15 parts by weight of phanerochaete chrysosporium liquid, 0.05-0.10 parts by weight of aspergillus niger liquid, 0.08-0.16 parts by weight of aspergillus oryzae liquid and 0.05-0.10 parts by weight of trametes versicolor liquid, and the dry weight of fermentation hyphae is more than 20g / L. The composite fungicide can be used for improving the composite fungicide speed of the xylose residue or the furfural residue as well as the methane production efficiency; the methane production efficiency is improved by 30-60%; the fermentation time is shortened; the start period is shortened by 3-10 days; and the invention is also good in the effects on the aspects of pretreatment of smashed straws, and methane production.

The invention discloses a method for removing a heavy metal pollutant from a water body by using phanerochete chrysosporium. The method comprises the following steps of: preparing spore suspension by using phanerochete chrysosporium spore powder; inoculating into a liquid culture medium for shake culture; filtering to obtain phanerochete chrysosporium pellets; adding the pellets into wastewater, adjusting the pH value of the wastewater, performing adsorption reaction under a constant temperature condition, filtering the wastewater after the reaction, and recovering the pellets to finish a removal process one time; cleaning the recovered pellets by using deionized water and then adding the cleaned pellets into culture solution for continuous culture so as to obtain phanerochete chrysosporium regenerative pellets; and adding the phanerochete chrysosporium regenerative pellets into heavy metal wastewater to be treated for cycling. By the method, hyphae are not required to be modified and pretreated and can be repeatedly used, the reaction condition is simple, and the water body of a complex composition can be treated.

The invention discloses a method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium, comprising the following steps: adding P.chrysosporium pellets into the wastewater containing cadmium and dichlorophenol, wherein the addition amount in each liter of wastewater is 0.3-0.5g in terms of the dry weight of the P.chrysosporium pellets; then, adjusting the pH value of the wastewater to 3.5-8.5, and performing an absorption degradation reaction at the constant temperature of 35-39 DEG C, wherein the revolving speed is controlled at 140-160rpm and the reaction time is not less than 36h during the reaction; and filtering the P.chrysosporium pellets after the reaction, thereby removing the cadmium and dichlorophenol in the wastewater simultaneously. The invention has the advantages of simple operation conditions, wide application range, no need of reformation and pretreatment for the adopted P.chrysosporium pellets, low cost, high microorganism utilization factor and the like, and is easy to implement.

The invention discloses a compound microbial preparation as well as a preparation method and application thereof. The preparation comprises phanerochaete chrysosporium balls, polyvinyl alcohol, silicon dioxide, activated carbon, sodium alginate and zeolite powder, which form the compound microbial preparation through crosslinking by a solution with sodium dihydrogen phosphate. The preparation method comprises the steps of preparing a gel liquid with the polyvinyl alcohol, sodium alginate, silicon dioxide and zeolite powder, mixing with a phanerochaete chrysosporium spore suspension with the activated carbon, then gradually dripping into the solution with the sodium dihydrogen phosphate so as to prepare embedded balls, and cultivating by a Kirk inorganic culture solution so as to prepare the compound microbial preparation. The compound microbial preparation has many gaps, high adsorption efficiency and good selectivity, the adsorbent has strong stability, the preparation method has the advantages of simple operation, low cost, affordability and so on, and the compound microbial preparation can be used for eliminating cadmium and 2, 4-dichlorophen in waste water.

The invention discloses a composting method capable of efficiently degrading antibiotics in livestock excrements. Degradation of a plurality of antibiotics, such as sulfonamides, tetracyclines, macrolides and fluoroquinolones, in a compost can be obviously promoted by adding an antibiotic degradation promoter with a specific using amount into a composting raw material and using specific composting steps, and meanwhile, available heavy metal content in the compost is reduced. The antibiotic degradation promoter consists of an activated carbon and an attapulgite, and can also be used with a biological activated carbon comprising the activated carbon and phanerochaete chrysosporium.

This invention discloses a method for producing fuel ethanol from a straw fibre raw material, comprising: (1) raw material selection; (2) disintegration; (3) pre-processing; (4) double-enzyme saccharifying; (5) sterilization; (6) enlargement culture and application of Pachysolen tannophilus P-01; (7) fermentation; (8) distillation; characterizing: the pre-processing employs white-rot fungi solid culture to decompose lignin. The procedures comprise: (1) bacteria selection: Phanerochete chrysosporium, which is donated by Shanghai hydnology institute of Chinese Academy of Agricultural Sciences, is a fugus, and can produce lignin decomposing enzymes under specific culture conditions, comprising lignin peroxidase, manganese dependent peroxidase, laccase, and so on; (2) specific culture media producing conditions: i. add 2g glucose, 0.3g ammonium chloride and 100g water to dry straw per 100g and mix homogenously, sterilize at 121Deg C for 2h, inoculate Phanerochete chrysosporium for culture decomposition; ii. conditions of culture decomposition: inoculation 5%, 37-39Deg C, pH normal, material to volume: 1:5 ( dry material weight: container volume), cycle 30 days. This invention can improve the ethanol yield and decease cost.

The invention relates to a straw-degrading composite microbial inoculum which is prepared by mixing a microbial inoculum No.I and a microbial inoculum No.II, wherein the microbial inoculum No.I comprises phanerochete chrysosporium, cacillus and streptomycete; and the microbial inoculum No.II comprises trichoderma, penicillium, aspergillus and actinomycete. The application method of the high-efficiency straw-degrading composite microbial inoculum in pretreatment of ethanol production comprises the following steps: soaking crushed straws in ammonia water to carry out cleaning treatment; adding a nutrient solution to prepare a fermentation substrate; spraying the high-efficiency straw-degrading composite microbial inoculum, and carrying out fermentation treatment for two days; and filtering,and washing with water to neutral, thus obtaining the pretreatment product for ethanol production. The invention has the following advantages: the treatment of soaking straws in ammonia water is beneficial to removing lignin, increasing the enzyme contact area of celluloses and hemicelluloses and improving the pretreatment efficiency; and by combining the microbial inoculum and the fermentation process to degrade straws, different microbes can secrete complementary enzymes in the degradation process, so that the substrate can be thoroughly degraded, thereby improving the biomass resource utilization rate. Besides, the invention is simple in process and convenient to implement, and can not do harm to the environment.

The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.

The invention belongs to the field of preparing biological coalbed methane by microorganisms, and discloses a compound microorganism bacterium agent of the biological coalbed methane prepared by coal bed organic impurities and an application of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises fermentation broths of four bacteria namely Phanerochaetechrysosporium, Penicilliumchrysogenum, Pseudomonasecpacia and Clostridiumacetobutylium. The compound microorganism bacterium agent disclosed by the invention has strong digestive ability to various celluloses, humus and various carbon-containing macromolecules in the coal bed. A coal added with the compound microorganism bacterium agent can accelerate the enrichment of methanogenus, shorten anaerobic digestion time and improve the output of the biological coalbed methane; and in addition, the synergy effects of the four microorganism strains are superior to the effect of any one, two or three microorganisms.

The invention relates to a method for selectively producing lignin-degrading enzymes by using phanerochaete chrysosporium. The invention provides a method for selectively producing lignin-degrading enzymes by using phanerochaete chrysosporium, which comprises the following steps: fermenting phanerochaete chrysosporium in a culture medium containing an immobilized culture carrier in a non-immersed state, and collecting a fermentation product, namely, a lignin-degrading enzyme. Experiments show that when the method is adopted for carrying out fermentation on a lignin-degrading enzyme, the lignin-degrading enzyme can be selectively obtained under air conditions without feeding pure oxygen or air containing high-concentration oxygen in the process of culturing but just by controlling the concentration of a carbon and nitrogen source in the culture medium and adding a carrier in a non-immersed state. The method disclosed by the invention is simple in condition control, and the obtained lignin-degrading enzyme is strong in selectivity.

The invention discloses a preparation method for an absorbent made from lignin-degrading bacteria modified straw, and belongs to the field of absorbent production. The preparation method comprises the following steps: dewaxing rape straw at first, and then adding a nutrient and a suspension without hyphae for sealed fermentation to obtain lignin-degrading bacteria modified straw; mixing the lignin-degrading bacteria modified straw with epoxy chloropropane for a cross-linking reaction, and then adding a trimethylamine alcoholic solution to obtain the absorbent eventually. The preparation method has the advantages that phanerochaete chrysosporium is utilized for degrading lignin in the rape straw to obtain cellulose and hemicellulose; the adsorption effect and the adsorption capacity of the prepared absorbent are high; moreover, the rape straw is utilized as a raw material for extracting natural cellulose; the preparation process is simple; the production cost is low.

The invention discloses maize stalk degradation and acidification species inoculant and a manufacture method thereof. Multiple species inoculant for degrading maize stalks comprises the following active components: aspergillus, trichoderma, penicillium and phanerochaete chrysosporium. The species inoculant breaks through the limitation that single fungus and clastic enzyme thereof cannot rapidly and efficiently decompose natural cellulose, and provides a key pretreatment technology for natural cellulose methane fermentation. By treating maize stalks through the method for 15 days continuously,the degradation rate of cellulose of the maize stalks reaches 42.1 percent, the degradation rate of hemicelluloses reaches 46.7 percent, the degradation rate of lignin reaches 28.5 percent, and the method has a wide application prospect when being applied to the field of methane preparation by taking cellulose and lignin, such as maize stalks, as raw materials.

The invention discloses a method used for accelerating lignin degradation in phanerochaete chrysosporium solid state fermentation, and belongs to the field of bioengineering. The method comprises following steps: s traw is grinded, and is treated by a proper amount of hydrogen peroxide, and then the straw is inoculated with phanerochaete chrysosporium used for lignin degradation, so that lignin degradation in solid state fermentation is accelerated, and cellulose is seldom degraded in the processes. The method is simple, no pollution is caused to the environment, and resource waste is not caused. Straw processed by the method can be used as a raw material in production of bioethanol and biobutanol, or as a biological feed, so that utilization value of straw is increased.

The invention discloses a microbial agent for pretreatment of straws and manure biogas. The microbial agent jointly comprises the following strains: 2-8*10<10>cfu / g of Penicillium glaucum, 2-8*10<10>cfu / g of Rhizopus oryzae, 2-8*10<10>cfu / g of Phanerochaete chrysosporium, 2-8*10<10>cfu / g of Aspergillus flavus and 2-8*10<10>cfu / g of Spirillospora. By virtue of the microbial agent, the mixed anaerobic fermentation process of straws and manure can be promoted, the fermentation period is shortened, the biodegradation of organic materials can be accelerated, and a preparation method of the microbial agent is simple and easily implemented, the content of flora in feedstock per unit is large and the activity is high. The microbial agent can be used for the fermentation of different manure and straw raw materials in biogas fermentation enterprises and thus the harmless, reduction and resource utilization of manure and straws are achieved.