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Preparation method for absorbent made from lignin-degrading bacteria modified straw

A technology of lignin-degrading bacteria and modified straw, applied in chemical instruments and methods, adsorption water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of low production cost, affecting cellulose adsorption capacity, and complex structure problems such as low production cost, improved adsorption effect, and simple preparation process

Inactive Publication Date: 2015-11-25
JIANGSU JINYU ENVIRONMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: aiming at the complex structure of lignin at present, the three-dimensional network structure is mainly connected with phenylpropane unit, and it is tightly bound with cellulose, which will affect the adsorption capacity of cellulose , provides a method for preparing a lignin-degrading bacteria-modified straw adsorbent. The invention first dewaxes rape stalks, and then adds nutrients and suspensions from which mycelia have been removed for sealed fermentation to obtain lignin-degrading bacteria modified The modified straw is mixed with epichlorohydrin for cross-linking reaction, and trimethylamine alcohol solution is added to mix to finally obtain a lignin-degrading bacteria-modified straw adsorbent. The present invention uses Phanerochaete chrysosporium to convert the straw The lignin is degraded to obtain cellulose and hemicellulose, the adsorption effect is good, the adsorption capacity is large, and the natural cellulose is extracted from rape straw, the preparation process is simple, and the production cost is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Firstly, the Phanerochaete chrysosporium was inoculated on the malt agar slant medium and placed in an incubator for 5 days at 35°C. After that, the white spores on the slant medium were scraped and put into 200 mL of sterilized distilled water. Obtain the spore suspension, and then pass the spore suspension through sterilized glass wool to remove the hyphae in the suspension, and place it in a freezer at 4°C for later use; take the rape straw, put it into a jet mill for pulverization, and then pass it 35 mesh standard sieve to obtain rape straw pellets, then put the straw pellets into the oven and dry at 70°C for 45 minutes; take 200 mL of the mixture of toluene and absolute ethanol at a volume ratio of 1:1 and pour into a 500 mL beaker , Take 30g of the above dried straw pellets and add it to the mixture of toluene and absolute ethanol, shake and soak for 25 minutes in a shaker, and then dry with a vacuum dryer to dewax the straw; the dewaxed rape straw Put it into a s...

example 2

[0028] Firstly, Phanerochaete chrysosporium was inoculated on the malt agar slant medium, and then placed in an incubator for 6 days at 40°C. After that, the white spores on the slant medium were scraped and put into 250 mL of sterilized distilled water. Obtain the spore suspension, and then pass the spore suspension through sterilized glass wool to remove the hyphae in the suspension, and place it in a freezer at 5°C for later use; take the rape straw, put it into a jet mill for pulverization, and then pass it 38 mesh standard sieve to obtain rape straw pellets, then put the straw pellets into an oven and dry at 75°C for 53 minutes; pour toluene and absolute ethanol into a 500mL beaker at a volume ratio of 1:1, and take 40g of the above The dried straw pellets are added to the mixture of toluene and absolute ethanol, shaken and soaked in a shaker for 30 minutes, and then dried with a vacuum dryer to dewax the straw; put the dewaxed rape straw into a sealed fermentation To the ...

example 3

[0033] Firstly, Phanerochaete chrysosporium was inoculated on the malt agar slant medium, and then placed in an incubator for 7 days at 45°C. Then, the white spores on the slant medium were scraped and put into 300 mL of sterilized distilled water. Obtain the spore suspension, then pass the spore suspension through sterile glass wool to remove the hyphae in the suspension, and place it in a freezer at 6°C for later use; take the rape straw, put it into a jet mill for pulverization, and then pass it 40 mesh standard sieve to obtain rape straw pellets, then put the straw pellets into the oven and dry at 80°C for 60 minutes; pour toluene and absolute ethanol into a 500mL beaker at a volume ratio of 1:1, and take 50g of the above The dried straw pellets are added to the mixture of toluene and absolute ethanol, shaken and soaked for 35 minutes on a shaker, and then dried with a vacuum dryer to dewax the straw; put the dewaxed rape straw into a sealed fermentation Tank, add 1000mL di...

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Abstract

The invention discloses a preparation method for an absorbent made from lignin-degrading bacteria modified straw, and belongs to the field of absorbent production. The preparation method comprises the following steps: dewaxing rape straw at first, and then adding a nutrient and a suspension without hyphae for sealed fermentation to obtain lignin-degrading bacteria modified straw; mixing the lignin-degrading bacteria modified straw with epoxy chloropropane for a cross-linking reaction, and then adding a trimethylamine alcoholic solution to obtain the absorbent eventually. The preparation method has the advantages that phanerochaete chrysosporium is utilized for degrading lignin in the rape straw to obtain cellulose and hemicellulose; the adsorption effect and the adsorption capacity of the prepared absorbent are high; moreover, the rape straw is utilized as a raw material for extracting natural cellulose; the preparation process is simple; the production cost is low.

Description

Technical field [0001] The invention discloses a preparation method of a lignin-degrading bacteria modified straw adsorbent, which belongs to the field of adsorbent manufacturing. Background technique [0002] Agricultural solid wastes mainly include crop straws, etc. They are mainly composed of lignocellulosic materials, of which the three major components are cellulose, hemicellulose, and lignin. Taking corn stalks as an example, the cellulose content is the highest, accounting for about 45% of the total, followed by hemicellulose at about 35%, and lignin at about 15%. However, the content of these components will vary depending on the growing region and crop type. Vary. Lignin is the second most abundant natural polymer compound on earth, second only to cellulose. Lignin accounts for about 20%-30% of the dry weight of wood, which shows that the role of lignin in the ecosystem is very important. Lignin belongs to the secondary metabolites of plants and has nothing to do with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C02F1/28C02F1/62C02F101/20
Inventor 李锦宇王志慧林大伟
Owner JIANGSU JINYU ENVIRONMENTAL ENG
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