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45 results about "Chrysosporium" patented technology

Chrysosporium is a type of hyaline hyphomycetes fungi in the family Onygenaceae. Chrysosporium colonies are moderately fast-growing, flat, white to tan to beige in color; they often have a powdery or granular surface texture. Hyaline, one-celled (ameroconidia) are produced directly on vegetative hyphae by non-specialized conidiogenous cells. Conidia are typically pyriform to clavate with truncate bases (6 to 7 by 3.5 to 4 um) and are formed either intercalary (arthroconidia), laterally (often on pedicels), or terminally.

Fertilizer based on oil-tea camellia cattail, preparation method and application thereof

The present invention belongs to the technical field of biomass resource utilization and microbial fertilizers, and specifically relates to a fertilizer based on oil-tea camellia cattail, a preparation method and its application. The fertilizer described in the present invention is prepared by a collaborative fermentation process of Aspergillus niger, Phanerochaete chrysosporium and Bacillus subtilis. The obtained fermentation product is separated into solid and liquid to obtain a solid, the solid being a solid fertilizer, and the liquid being a foliar fertilizer after post-treatment. Both can be used for the cultivation of crops such as tangerines and oil-tea camellias. Based on the microbial collaborative fermentation process, the content of anti-nutritional factors tannin and tea saponin in the obtained solid fertilizer is significantly reduced, which is less than 1.8 g / kg and 0.1% respectively on a dry basis, while maintaining a seed germination index higher than 95%. According to a planting comparative test, the average yield per mu of oil-tea camellias using the solid fertilizer and liquid fertilizer obtained in this application increased by 12%. The average yield per mu of tangerines increased by about 11%, and the sugar, vitamin C, and trace element contents in tangerines were higher than those using traditional fertilizers.
Owner:HUANGSHAN BAWEI ECOLOGICAL AGRI TECH CO LTD

Method and application of fungus-enzyme synergistic fermentation of bagasse pith

PendingCN120616028AFungiFood processingPhanerochaeteSucrose
The invention relates to the technical field of biology, in particular to a method and application of fungus-enzyme synergistic fermentation of bagasse pith, and the method comprises the following steps: (1) adjusting the water content of the bagasse pith to 60-70%, and adjusting the pH to 4.0; (2) adding the following components according to dry matter mass: 3% of phanerochaete chrysosporium liquid with the concentration of 1.5 * 10 < 8 > CFU / ml, and uniformly mixing; 1% of sucrose; the cellulase liquid is 20000U / kg, and the cellulase liquid is prepared by dissolving crude enzyme obtained by centrifuging after trichoderma hedgehog is fermented to produce enzyme in a PBS (phosphate buffer solution); and (3) putting the mixture into a fermentation tank, and performing sealed low-oxygen fermentation for 30 days to obtain the bagasse pith feed. According to the bagasse pith feed obtained by the method, the nutritional value of the bagasse pith can be improved, the fermentation quality is improved, the digestion potential is improved, the storage time is prolonged, and high-quality feed application of the bagasse is realized.
Owner:GUANGXI UNIV

Filtered mud composting method based on synergistic interaction of sargassum fusiforme and complex microbial inoculants

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.
Owner:GUANGXI UNIV

Microbial agent for increasing manure compost temperature and application of microbial agent in high-temperature compost

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.
Owner:AGRI MASCH EQUIP & ENG RES INST ANHUI ACAD OF AGRI SCI

Tremella aurantialba cultivation and planting method and closed square cabin special for cultivation and planting

The invention discloses a tremella auramtialba cultivation and planting method and a closed square cabin special for cultivation and planting, and relates to the technical field of planting. According to the tremella aurantialba cultivation and planting method and the closed square cabin special for cultivation and planting, a cabin body, a rotary cultivation mechanism and a supply mechanism are included, the rotary cultivation mechanism comprises a driver and an operation planting assembly, the operation planting assembly comprises a conveying belt, and the surface of the conveying belt is fixedly connected with a semicircular strip-shaped supporting piece; the top end of the semicircular strip-shaped supporting piece is fixedly connected with a planting disc, the supplementing mechanism comprises a lifting adjuster, a liquid storage tank and a spraying assembly, the spraying assembly comprises a first air cylinder, a second air cylinder and a third air cylinder, and the first air cylinder, the second air cylinder and the third air cylinder are sequentially installed at the output end of the lifting adjuster; the telescopic end of the first air cylinder, the telescopic end of the second air cylinder and the telescopic end of the third air cylinder are all fixedly provided with spraying heads, the adjustable purpose is achieved, the height is adjusted according to strain growth, water is supplemented softly, impact is reduced, and safety and reliability are achieved.
Owner:GANSU ACAD OF SCI INST OF BIOLOGY +1

Gene, primer set for identifying chrysosporium and application thereof

The application provides a gene, a primer set and an application for identifying golden ear, and belongs to the technical field of biological detection. The application obtains specific gene fragments of two species contained in the golden ear through large-scale whole genome comparison analysis, designs species-specific primers based on the specific gene fragments, and further realizes identification of the effective species of the golden ear. The identification is extremely crucial in the production process of the effective species of the golden ear, and can maximize the guarantee of the fruiting rate in the production of the golden ear.
Owner:YUNNAN MINZU UNIV

Stevia rebaudiana residue fermented feed additive for improving livestock and poultry productivity, and preparation method therefor

PCT designated stageWO2025194438A1Animal feeding stuffBiotechnologyPhanerochaete
A Stevia rebaudiana residue fermented feed additive for improving livestock and poultry productivity, a preparation method therefor, and a use thereof. Raw materials of the Stevia rebaudiana residue fermented feed additive comprise: Stevia rebaudiana residue, fermented brown sugar, a microbial agent, and water. The microbial agent is composed of EM bacteria, white rot fungus and Phanerochaete chrysosporium, in a ratio of 1:1:1.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Efficient lignocellulose degradation compound strain and application thereof in production of methane by fermenting straws

PendingCN121975632AFungiHydrolasesBiotechnologyPhanerochaete
The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Amelanospora sp. HLC15 and application thereof

PendingCN121086892ABiocidePlant growth regulatorsBiotechnologyNigrospora oryzae
The invention discloses a strain of rice endophytic fungus nigrospora oxyspora HLC15. The preservation number of the rice endophytic fungus nigrospora oxyspora HLC15 in the Guangdong Microbiological Culture Collection Center is GDMCC (China General Microbiological Culture Collection Center) No.66882; the strain has relatively strong phosphate solubilizing and IAA (indoleacetic acid) producing capacities and cadmium tolerance, can obviously promote the growth of host plants, increase the chlorophyll content of the host plants and relieve the harm of cadmium pollution to crops under the stress of cadmium when being inoculated with HLC15, and is of great significance in the growth and safe production of the crops in cadmium-polluted soil.
Owner:KUNMING UNIV OF SCI & TECH

Irpex bacteria, terpenoids and application of terpenoids in resisting tobacco pathogenic bacteria

The invention discloses Irpex lacteus YYY-16, and the preservation number of the Irpex lacteus YYY-16 is GDMCC No: 67140. The invention further discloses a terpenoid and a preparation method thereof. The terpenoid has a structure shown in a formula I or a formula II. The invention further discloses application of the terpenoid in preparing tobacco pathogenic bacterium resisting drugs.
Owner:CHINA TOBACCO YUNNAN IND

Microbial agent-containing base fertilizer for brassicaceous plant broccoli

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.
Owner:CHIZHOU WANMUYUAN AGRICULTURAL TECHNOLOGY DEVELOPMENT CO LTD

Rabbit manure organic fertilizer and preparation method thereof

The invention provides a rabbit manure organic fertilizer and a preparation method thereof, and the rabbit manure organic fertilizer is prepared by using rabbit manure, rapeseed meal and wheat straw as raw materials through fermentation with a complex microbial inoculant; wherein the complex microbial inoculant is prepared by mixing a phanerochaete chrysosporium bacterial liquid, a bacillus subtilis bacterial liquid and a Thermus thermophilus bacterial liquid. Furthermore, the rabbit manure organic fertilizer is prepared by fermenting the complex microbial inoculant provided by the invention, the degradation of cellulose substances can be remarkably promoted, and the prepared fertilizer has a relatively high seed germination index when being used for germinating seeds. In addition, after fermentation, the content of copper and zinc in a water-soluble state and a weak acid extraction state in the raw materials is reduced, the risk that plants absorb heavy metals is reduced, and the application safety of the organic fertilizer is improved.
Owner:WUHAN ANIMAL DISEASE PREVENTION & CONTROL CENT +1

Composite microbial agent for decomposing forest litter, method for preparing the same, and method for applying the same

PendingCN122382054APhanerochaeteMicrobial agent
The application discloses a kind of for decomposing forest litter composite microbial inoculant, preparation method and its application method, it is related to litter decomposition technical field.The application includes a kind of for decomposing forest litter composite microbial inoculant preparation method, comprising the following steps: S1.to Phanerochaete chrysosporium, Trichoderma reesei and ropegillus catenulatum are respectively carried out directional metabolism preactivation;S2.the three preactivation wet bacteria bodies obtained in step S1 are mixed according to the fresh weight ratio of Phanerochaete chrysosporium:Trichoderma reesei:ropegillus catenulatum 3:2:1, obtain mixed bacteria body;S3.mixed bacteria body is mixed with the straw carrier that is preprocessed by steam explosion according to the mass ratio of bacteria body fresh weight:carrier dry weight 1:2, under the condition of temperature 28 DEG C, relative humidity 92~95%, stationary culture 72 hours, carry out phased micro-perturbation aeration, construct physical symbiosis;S4.the material obtained in step S3 is dried to 15% moisture content at 40 DEG C, obtain composite microbial inoculant;Efficient and stable decomposition of pine forest litter is realized.
Owner:YUNNAN UNIV

Soaking pool raw material circulating device for tremella aurantialba fungus production

The utility model relates to the field of tremella aurantialba production, in particular to a soaking pool raw material circulating device for tremella aurantialba production, which comprises a bottom plate, support legs arranged at the lower end of the bottom plate, a soaking pool body arranged at the upper end of the bottom plate, a vertical column fixedly connected to the upper end of the bottom plate, a top plate arranged at the upper end of the vertical column, and a transverse plate arranged in the bottom plate. A lifting module is arranged at the upper end of the transverse plate, a servo motor is arranged at the upper end of the top plate and drives the lifting module to work, a lifting plate is arranged in the lifting module, a through groove is formed in the lifting plate in a penetrating mode, two first bearing seats are arranged at the upper end of the lifting plate, and a rotating shaft is rotationally connected into each first bearing seat. A rotary motor is arranged at the upper end of the lifting plate; the first gear and the second gear are in transmission linkage through the synchronous belt, the toothed plate is meshed with the first gear and the second gear to achieve horizontal reciprocating circulation movement, the stirring paddle evenly stirs raw materials in the soaking pool body, and production efficiency and product quality are improved.
Owner:SHAANXI YUZHI JINYU BIOTECHNOLOGY CO LTD

Method for treating dichlorophenol wastewater by activated sludge

The present application relates to a kind of active sludge treatment dichlorophenol wastewater method, it is related to wastewater treatment technical field.The present application includes the following steps: (1) domestication of active sludge: adding bacterial body to active sludge, obtain mixed active sludge;Afterwards, mixed active sludge is added to domestication culture solution, domestication is obtained, and domesticated active sludge is obtained;(2) the treatment of dichlorophenol wastewater: domesticated active sludge is mixed with the dichlorophenol wastewater to be treated, and the treatment of dichlorophenol wastewater is realized;Wherein, the bacterial body used is the mixed bacteria of yellow phanerochaete chrysosporium and corynebacterium glutamicum.The present application can realize the efficient degradation of dichlorophenol, further guarantee the degradation effect of TOC in wastewater, the degradation product is safe, and it has the significant advantage of low energy consumption.
Owner:ZHENGZHOU UNIVERSITY OF AERONAUTICS

An ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof

PendingCN122104674AAgriculture tools and machinesBacteriaSporelingPhanerochaete
The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.
Owner:SINO-SINGAPORE RUIMEI (TIANJIN) ENVIRONMENTAL PROTECTION TECH CO LTD +1

Iron carrier mediated symbiotic degradation bacterial agent for repairing petroleum hydrocarbon polluted soil

ActiveCN121495756AFungiBacteriaPhanerochaeteMicrobial agent
The invention discloses a siderophore-mediated symbiotic degradation bacterial agent for remediation of petroleum hydrocarbon contaminated soil. The microbial agent is formed by compounding a high-yield siderophore bacterium pseudomonas aeruginosa DG-1 with the strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No.28382 and a fungus white rot fungus BNCC336258 according to the volume ratio of 1: (2 to 4), and through the siderophore-mediated bacterium-fungus synergistic metabolism effect, the remediation efficiency of the petroleum hydrocarbon contaminated soil under the iron limiting condition (the free iron concentration is smaller than or equal to 1.0 * 10 <-18 > M) is improved. The microbial agent is suitable for treating low-concentration polluted soil with the petroleum hydrocarbon concentration of 0.5-5 wt%, and the total petroleum hydrocarbon removal rate after remediation can reach 80% or above.
Owner:HEBEI UNIV OF TECH +2

Culture method for inducing mycelium to secrete specific extracellular polymeric substance and application of culture method in sand fixation

PendingCN121718435AFungiOther chemical processesBiotechnologyPhanerochaete
The invention discloses a culture method for inducing mycelia to secrete specific extracellular polymeric substances and application of the culture method in sand stabilization, and relates to the field of biotechnology and environmental materials.The culture method comprises the following steps that phanerochaete chrysosporium is inoculated into a liquid fermentation culture medium, and fermentation is conducted under the conditions that the pH ranges from 4.5 to 5.5, the temperature ranges from 35 DEG C to 37 DEG C and shaking culture is conducted; the liquid fermentation culture medium contains tween-80 with the concentration of 0.5-4 g / L and hydrogen peroxide with the concentration of 0.05-0.15 mM as an inducer combination; after the fermentation is finished, separating to obtain fermentation supernate, namely the functional extracellular polymeric substance fermentation liquor; according to the culture method for inducing the mycelium to secrete the specific extracellular polymeric substance and the application of the culture method in sand stabilization, the functional EPS is obtained from the source of regulating and controlling microbial metabolism, and the limitation that a traditional mycelium material only depends on the physical form is broken through.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Preparation method of loropetalum chinense extract and application of loropetalum chinense extract in preparation of products for wound healing

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for wound healing, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and composite biological bacteria, and carrying out enzymolysis to obtain an enzymolysis product; the composite biological bacterium is a mixture of phanerochaete chrysosporium and streptomyces griseus; carrying out gradient alcohol extraction on the enzymolysis product, merging alcohol extracts, extracting with petroleum ether, and concentrating an organic phase to obtain a component 1; the component 1 is adsorbed by macroporous adsorption resin, the resin is washed with deionized water and then eluted with an eluent, 5-35 min eluent is collected, combined and dried, and the component 1 is obtained. The elution phase is a mixture of cyclohexane, ethyl acetate and acetonitrile. The loropetalum chinense extract is prepared through a specific preparation method, and the wound healing effect is remarkably improved.
Owner:BEIJING GUSHEN LIFE HEALTH TECH CO LTD

Schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.
Owner:YANGZHOU UNIV

A solid-state fermentation inoculant, its preparation method and application

PendingCN122081081AFungiContaminated soil reclamationBiotechnologyPhanerochaete
This invention provides a solid-state fermentation inoculant, its preparation method, and its application. The preparation method of the solid-state fermentation inoculant involves inoculating *Phanerochaete chrysosporium* into a seed carrier and culturing it in a sterile, pure oxygen environment. Under pure oxygen conditions, the inoculated *Phanerochaete chrysosporium* exhibits a significantly increased reproduction rate, forming a high-density microbial community. Simultaneously, the continuous and stable nutrient supply from the seed carrier enables the inoculant to quickly establish a competitive advantage upon entering materials containing other microorganisms. By seizing nutrients and oxygen resources, it inhibits the growth and reproduction of other microorganisms, ultimately increasing the abundance of white-rot fungi in the fermented product, thereby enhancing the degradation efficiency of petroleum hydrocarbons in the soil. Compared to traditional solid-state fermentation methods, the inoculant production process is very simple and low-cost. The solid-state fermentation inoculant product of this invention has a high microbial density and a relatively large number of target strains, resulting in higher efficiency in soil pollution remediation and increased soil fertility.
Owner:YANGTZE UNIVERSITY

An anti-inflammatory repair composition and methods of making and using the same

The present application relates to the technical field of daily chemical industry, and in particular to an anti-inflammatory repair composition, a preparation method and application thereof. The composition comprises a rhizoma anemarrhenae extract obtained by fermenting rhizoma anemarrhenae by a chrysosporium fungus. The present application provides a composition that enhances the moisture retention capacity of the skin, helps form a moisturizing barrier, and prevents moisture loss from the skin. The composition contains flavonoids and polysaccharide compounds, which can scavenge free radicals and play an antioxidant role, thereby slowing down skin aging and protecting the skin from damage caused by environmental stress. The formula of the present application inhibits the formation of melanin, helps improve uneven skin tone, and enhances the brightness of the skin.
Owner:广州宾缇生物科技有限公司

Complex microbial inoculant, preparation method and application

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant, a preparation method and application. The preparation method comprises the following steps: selecting an anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and a phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776, inoculating the anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and the phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776 into a carrier, and mixing and compounding to obtain the composite microbial inoculant, so that the composite microbial inoculant has good air permeability and flexibility due to the pore structure of the carrier buckwheat hull; the mass transfer efficiency and the adsorption effect of oxygen molecules can be improved through synergistic interaction; when the complex microbial inoculant is applied to degradation of compost and agricultural and forestry waste, the enzyme activity and high temperature resistance of the compost and the agricultural and forestry waste can be effectively improved, synergistic complementation on degradation of lignin, cellulose and hemicellulose is achieved, and the degradation efficiency of the lignin, the cellulose and the hemicellulose is high. In the mixing application of vinasse and straw, the temperature can be rapidly increased, and the temperature can be kept at 60-80 DEG C or above for 2 weeks.
Owner:JIANGSU ENVIRONMENTAL ENG TECH CO LTD +2

Self-adaptive regulation and control natural eurotium cristatum growing method of coffee eurotium cristatum

The invention relates to the technical field of biology and food, and discloses a self-adaptive regulation and control coffee eurotium cristatum natural eurotium cristatum growing method which comprises the following steps: soaking green coffee beans, removing silver skins on the surfaces of the green coffee beans, and performing high-pressure sterilization treatment; inoculating the sterilized coffee beans with a microbial inoculum containing aspergillus cristatus strains for solid state fermentation; baking the fermented coffee beans to obtain finished coffee beans; the Latin literature name of the aspergillus cristatus is Aspergillus cristatus, and the strain number of the aspergillus cristatus is GDMCC 67444. According to the method, the green coffee beans are subjected to single-bacterium / mixed-bacterium fermentation treatment through specific strains, the edible safety and functionality of the green coffee beans are improved, core sensory quality such as acidity and fragrance of coffee is directionally improved, compared with unfermented green coffee beans, the quality advantage is remarkable, the fermentation process is stable and controllable, the actual production requirements of the coffee processing industry are met, and the method is suitable for popularization and application. The method has outstanding practical value and popularization prospect.
Owner:HAINAN UNIV

Phanerochaete chrysosporium and application thereof in degradation of corn stalks for fermenting production of erucamide

ActiveCN120555201BBiotechnologyCellulose
The application discloses a white-cyst Irpex lacteus and application of the white-cyst Irpex lacteus in degradation of corn stalks and fermentation production of erucic acid amide. The white-cyst Irpex lacteus is classified and named as Irpex lacteus J2, and has been preserved in the China Center for Type Culture Collection on November 24, 2023, with a preservation number of M20232318. The strain has a complete lignocellulose-degrading enzyme system, and can produce erucic acid amide by taking corn stalks as the only carbon source.
Owner:BEIJING UNIV OF AGRI

Biomass charcoal adsorption material, preparation method and application of biomass charcoal adsorption material in pesticide degradation

The invention provides a biomass charcoal adsorption material and a preparation method thereof, and belongs to the field of pesticide degradation. The preparation method comprises the following steps: preparing biochar, preparing modified biochar and compounding. The compounding method comprises the following steps: adding modified biochar, bacillus subtilis, pseudomonas FH2, bacillus licheniformis and phanerochaete chrysosporium into deionized water, stirring, filtering, adding a filter cake into a chitosan solution, then adding Tween 60, stirring for 2-3 hours at the speed of 500-600r / min, slowly dropwise adding a glutaraldehyde solution, continuously stirring for 8-10 hours after dropwise adding, finally centrifuging at the speed of 7000-8000r / min, and drying to obtain the biochar composite material. Collecting solids, and drying to obtain a biomass charcoal adsorption material; the biomass charcoal adsorption material disclosed by the invention has broad spectrum on pesticide degradation, the degradation rate on trichlorfon reaches 87 to 89 percent, the degradation rate on acetamiprid reaches 89 to 93 percent, the degradation rate on deltamethrin reaches 93 to 96 percent, and the degradation rate on DDT reaches 65 to 70 percent.
Owner:WEIFANG UNIV OF SCI & TECH

An MBR reactor and its application

This invention discloses an MBR reactor, belonging to the field of wastewater treatment technology. The MBR reactor of this invention includes an aerobic zone and a membrane module. Aerobic sludge is added to the aerobic zone, and the membrane module has a membrane. Functional mycelial balls are inoculated onto the aerobic sludge. The membrane contains inorganic particles. The method for preparing the functional mycelial balls includes: a) providing a mixed spore suspension of Aspergillus niger and Phanerochaete chrysosporium, adding biodegradable polymer particles, and culturing; b) adding a mixed bacterial suspension of Bacillus belye and Bacillus argentis to the mixed spore suspension, and culturing again. The main purpose of this invention is to reduce membrane fouling, improve the self-cleaning performance of the membrane, and increase the removal efficiency of pollutants such as residual antibiotics in wastewater.
Owner:NANJING INNOVATION CENT FOR ENVIRONMENTAL PROTECTION IND

Composite organic fertilizer and preparation method thereof

The present application belongs to the technical field of waste conversion fertilizer, and provides a kind of compound organic fertilizer and its preparation method, the method steps are as follows: S1, by weight, rice straw 27-30 parts, bran 9-10 parts and animal manure 8-9 parts are mixed to obtain initial raw material;S2, 0.85-0.9 parts of oxidized modified straw biochar and 0.5-0.55 parts of ammoniated superphosphate are added into the initial raw material at the same time and mixed, and then the moisture content is adjusted to 53±3%, to obtain the base material;S3, add phanerochaete chrysosporium suspension and bacillus subtilis activation solution to the base material and mix evenly to obtain fermentation material;S4, the fermentation material is composted and fermented for more than 28 days to obtain the compound organic fertilizer.The present application shortens the composting time to improve the preparation efficiency of organic fertilizer, and further reduces the phytotoxicity of the prepared organic fertilizer.
Owner:NANYANG ZHAOFU AGRI TECH DEV CO LTD

Chrysanthemum breeding method

The application provides a chrysosporium inosporum breeding method and relates to the technical field of edible mushroom cultivation. The breeding method is as follows: the chrysosporium fruiting body is hung and cultured, and after the spores in the fruiting body are separated out, the spores are moved to a flat plate culture medium to culture the chrysosporium single colony, two chrysosporium single colonies are randomly picked and cross lines are drawn on the same clean flat plate culture medium to carry out hybridization culture, and the liquid strain of phanerochaete chrysosporium is simultaneously introduced as a mycelium growth auxiliary, is evenly smeared on the culture medium, and the ear is induced. The breeding method solves the technical problems of the existing cultivation method, such as complex operation, tedious steps, high requirement for equipment and long cultivation time, and realizes the technical effects of fast spore culture speed, simple operation, low equipment requirement and meeting the needs of factory production.
Owner:DONGZHI HESHUISHAN TECH CO LTD

Selenium source regulation and control method for directionally producing high-value selenium-rich phellinus igniarius mycelium or sporocarp

PendingCN122030183ACultivating equipmentsMushroom cultivationBiotechnologyMedicinal mushroom
The invention discloses a selenium source regulation and control method for directionally producing high-value selenium-rich phellinus igniarius mycelium or sporocarp, and belongs to the technical field of edible and medicinal fungus cultivation and microbial fermentation. The invention provides a target-oriented differential selenium source strategy aiming at the technical contradiction that a single selenium source cannot meet the requirements of different growth stages of phellinus igniarius in the prior art. When the phellinus igniarius mycelium is used as a target product, a fermentation strategy of adding selenium-enriched yeast in the whole process is adopted; when phellinus igniarius sporocarp is used as a target product, a staged strategy is adopted: selenium-enriched yeast is added in a mycelium culture stage, and sodium selenite is added in a sporocarp culture stage. According to the method, the rule that the mycelium stage is adaptive to organic selenium and the sporocarp stage is adaptive to inorganic selenium is defined for the first time, and through accurate stage-selenium source matching, the selenium content, yield and comprehensive quality of the mycelium or the sporocarp are synchronously improved successfully on the premise that mycelium growth is hardly inhibited.
Owner:SHANGHAI ACAD OF AGRI SCI