62 results about "Chrysosporium" patented technology

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the field of microorganisms, and particularly relates to a biochar-based immobilized microbial agent for promoting litter decomposition and a preparation method thereof.The biochar-based immobilized microbial agent for promoting litter decomposition comprises a biochar-based culture medium and a microbial seed solution, the active ingredient of the microbial seed solution is phanerochaete chrysosporium, and the preservation number is SHBCC D13411. According to the invention, the adsorption efficiency of the microbial agent is enhanced through the iron-modified biochar, and a habitat suitable for development and reproduction is provided for microorganisms; on the other hand, the biochar has very strong hygroscopicity, water molecules can be adsorbed on the surface, and a suitable growth environment is provided for microorganisms, so that the microorganisms are promoted to decompose litters.

The present invention belongs to the technical field of biomass resource utilization and microbial fertilizers, and specifically relates to a fertilizer based on oil-tea camellia cattail, a preparation method and its application. The fertilizer described in the present invention is prepared by a collaborative fermentation process of Aspergillus niger, Phanerochaete chrysosporium and Bacillus subtilis. The obtained fermentation product is separated into solid and liquid to obtain a solid, the solid being a solid fertilizer, and the liquid being a foliar fertilizer after post-treatment. Both can be used for the cultivation of crops such as tangerines and oil-tea camellias. Based on the microbial collaborative fermentation process, the content of anti-nutritional factors tannin and tea saponin in the obtained solid fertilizer is significantly reduced, which is less than 1.8 g / kg and 0.1% respectively on a dry basis, while maintaining a seed germination index higher than 95%. According to a planting comparative test, the average yield per mu of oil-tea camellias using the solid fertilizer and liquid fertilizer obtained in this application increased by 12%. The average yield per mu of tangerines increased by about 11%, and the sugar, vitamin C, and trace element contents in tangerines were higher than those using traditional fertilizers.

The invention belongs to the technical field of biological materials, and particularly relates to a preparation method of a KOH activated fungal mycelium biochar / chitosan composite material. According to the preparation method disclosed by the invention, Phanerochaete chrysosporium mycelium is used as a carbon source, and KOH chemical activation and chitosan surface modification are sequentially carried out, so that the charcoal / chitosan composite material CHKBC is prepared. The CHKBC material prepared by the method is large in specific surface area, developed in pore structure, rich in mesopores and excellent in adsorption performance and regeneration performance; the specific surface area is up to 3650-3920m < 2 > / g, the average pore size is 2-4nm, and both the specific surface area and the average pore size are higher than those of precursor biochar and activated biochar; the maximum adsorption capacity of tetracycline TC is 650-690 mg / g, which is far higher than that of KBC and BC. After five times of recycling, the average regeneration efficiency is higher than 90%, and the regeneration performance is good.

The invention relates to the technical field of biology, in particular to a method and application of fungus-enzyme synergistic fermentation of bagasse pith, and the method comprises the following steps: (1) adjusting the water content of the bagasse pith to 60-70%, and adjusting the pH to 4.0; (2) adding the following components according to dry matter mass: 3% of phanerochaete chrysosporium liquid with the concentration of 1.5 * 10 < 8 > CFU / ml, and uniformly mixing; 1% of sucrose; the cellulase liquid is 20000U / kg, and the cellulase liquid is prepared by dissolving crude enzyme obtained by centrifuging after trichoderma hedgehog is fermented to produce enzyme in a PBS (phosphate buffer solution); and (3) putting the mixture into a fermentation tank, and performing sealed low-oxygen fermentation for 30 days to obtain the bagasse pith feed. According to the bagasse pith feed obtained by the method, the nutritional value of the bagasse pith can be improved, the fermentation quality is improved, the digestion potential is improved, the storage time is prolonged, and high-quality feed application of the bagasse is realized.

The invention discloses a preparation method of a slow-release fertilizer based on a silkworm excrement carrier simultaneously loaded with urea and a urease inhibitor. The method comprises the following steps: firstly, by taking silkworm excrement as a raw material, adding phanerochaete chrysosporium, aspergillus niger and bacillus subtilis complex microbial inoculants for fermentation; and fermenting at 30-35 DEG C for 3-4 days. After fermentation is finished, weighing the crushed fermented silkworm excrement, urea and a urease inhibitor according to the mass ratio of (6-7): 1: 0.08, adding water, and stirring and mixing, so that the urea and the urease inhibitor are fully loaded into a pore structure of the silkworm excrement; and drying and granulating to obtain the silkworm excrement-based slow-release fertilizer. And the loaded fertilizer realizes slow release of nitrogen by virtue of the synergistic effect of a silkworm excrement hydrogen bond polymerization structure and the urease inhibitor, has a remarkable effect on crops with long growth cycle and high nitrogen demand, and has both environmental protection and economic benefits.

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention discloses a tremella auramtialba cultivation and planting method and a closed square cabin special for cultivation and planting, and relates to the technical field of planting. According to the tremella aurantialba cultivation and planting method and the closed square cabin special for cultivation and planting, a cabin body, a rotary cultivation mechanism and a supply mechanism are included, the rotary cultivation mechanism comprises a driver and an operation planting assembly, the operation planting assembly comprises a conveying belt, and the surface of the conveying belt is fixedly connected with a semicircular strip-shaped supporting piece; the top end of the semicircular strip-shaped supporting piece is fixedly connected with a planting disc, the supplementing mechanism comprises a lifting adjuster, a liquid storage tank and a spraying assembly, the spraying assembly comprises a first air cylinder, a second air cylinder and a third air cylinder, and the first air cylinder, the second air cylinder and the third air cylinder are sequentially installed at the output end of the lifting adjuster; the telescopic end of the first air cylinder, the telescopic end of the second air cylinder and the telescopic end of the third air cylinder are all fixedly provided with spraying heads, the adjustable purpose is achieved, the height is adjusted according to strain growth, water is supplemented softly, impact is reduced, and safety and reliability are achieved.

The application provides a gene, a primer set and an application for identifying golden ear, and belongs to the technical field of biological detection. The application obtains specific gene fragments of two species contained in the golden ear through large-scale whole genome comparison analysis, designs species-specific primers based on the specific gene fragments, and further realizes identification of the effective species of the golden ear. The identification is extremely crucial in the production process of the effective species of the golden ear, and can maximize the guarantee of the fruiting rate in the production of the golden ear.

A Stevia rebaudiana residue fermented feed additive for improving livestock and poultry productivity, a preparation method therefor, and a use thereof. Raw materials of the Stevia rebaudiana residue fermented feed additive comprise: Stevia rebaudiana residue, fermented brown sugar, a microbial agent, and water. The microbial agent is composed of EM bacteria, white rot fungus and Phanerochaete chrysosporium, in a ratio of 1:1:1.

The invention relates to the technical field of soil improvement, in particular to a soil conditioner for improving desert saline-alkali soil and application of the soil conditioner. The soil conditioner is prepared from 5.5 to 7.5 * 10 < 4 > kg of yellow water, 5 to 6 * 10 < 3 > kg of crop straw, 60 to 76 kg of phanerochaete chrysosporium, 50 to 60 kg of bacillus amyloliquefaciens J10, 30 to 46 kg of trichoderma viride, 30 to 50 kg of lactobacillus kefir CP423, 1 to 1.2 * 10 < 4 > kg of organic fertilizer, 0.8 to 1.2 * 10 < 3 > kg of amino acid water-soluble fertilizer, 25 to 35 kg of nitrogen fertilizer and 8 to 12 kg of phosphate fertilizer. After the soil conditioner is applied to ecological improvement, the content of organic matter, total nitrogen, total phosphorus, alkali-hydrolyzable nitrogen and available phosphorus can be effectively increased, the soil quality is remarkably improved, and the yield and quality of planted crops can be remarkably improved.

The present invention belongs to the field of microbial technology and specifically relates to a composite microbial agent for promoting lignocellulose degradation and humus production during mulberry twig and cow dung composting, and its application. The composite microbial agent comprises a mixture of strains B1, F1, and F2, wherein the ratio of effective viable bacteria among strains B1, F1, and F2 is 1:1:1. The deposit number of strain B1 is GDMCC No. 65049; the deposit number of strain F1 is GDMCC No. 65050; and the deposit number of strain F2 is GDMCC No. 65051. The Bacillus cereus B1, Phanerochaete chrysosporium F1, and Sphaerotheca fusca F2 in the composite microbial agent are all effective fertilizer compost-promoting microorganisms. These three microorganisms can synergize with each other during aerobic composting, fully achieving the conversion and degradation of raw materials and producing high-quality organic fertilizer. Furthermore, the composite microbial agent can promote lignocellulose degradation and humus production during composting.

A recombinant polypeptide and a recombinant cell both expressing an aromatic amino acid degrading enzyme, and a use of the recombinant polypeptide and the recombinant cell, relating to the field of biotechnology. The sequence of the aromatic amino acid degrading enzyme XAL derived from Phanerochaete chrysosporium is taken as a starting sequence, a series of XAL mutations that can improve the enzyme activity and stability are found by means of screening and verification of mutation sites, the mutants are introduced into the genetically engineered bacteria, and then degradation activity verification is performed; and it is found that compared with a control strain of a transformed non-mutant enzyme, the constructed recombinant strain has the amino acid degradation activity significantly improved. The present invention further relates to a use of a recombinant XAL polypeptide or a recombinant cell, and a composition of the recombinant XAL polypeptide or the recombinant cell for the treatment and industrial purposes.

The invention belongs to the technical field of rice planting, and particularly relates to a rice planting method for reducing soil microplastic pollution. By applying the soil conditioner, the pea crop rotation, the compost treatment and the specific irrigation strategy, the soil micro-plastic pollution can be effectively reduced; the pea plants can enrich micro-plastic particles in the soil through root exudates and self metabolic activities to a certain extent, and the pea plants are subsequently composted, so that the micro-plastic particles enriched in the pea plants can be further degraded; biochar in the soil conditioner can adsorb micro-plastic particles, the free state of the micro-plastic particles in soil is reduced, and the phanerochaete chrysosporium, the rhodococcus fence celebrating and the trichoderma harzianum can synergistically degrade the micro-plastic particles; the dry-wet alternate cycle treatment can further reduce the absorption of the rice plants to the micro-plastics, can promote the development of rice roots and the absorption of nutrients, and improves the stress resistance of the rice.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention discloses an algicidal bacterium TL3 with efficient algicidal activity and application thereof, the algicidal bacterium TL3 is named as Enterobacter cloacae TL3 and is preserved in Guangdong Microbial Culture Collection Center located at No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province, the preservation number is GDMCC No: 65777, and the preservation date is January 13, 2025; the invention also provides an algicidal bacterial agent containing the algicidal bacteria TL3 for degrading or inhibiting water-blooming cyanobacteria. The algicidal bacterium TL3 is obtained by screening and separating in the algicidal period by taking the algicidal habitat of the Lake Taihu as a strain resource library of the efficient algicidal bacterium, has an efficient inhibition effect on microcystis aeruginosa, has a certain algicidal effect on chrysosporium oospermum, cuscuta larakii and anabaena pseudosciaena, is used for various types of cyanobacterial bloom water bodies, and has a good algicidal effect on the water bodies. The application prospect is wide.

The invention discloses a strain of rice endophytic fungus nigrospora oxyspora HLC15. The preservation number of the rice endophytic fungus nigrospora oxyspora HLC15 in the Guangdong Microbiological Culture Collection Center is GDMCC (China General Microbiological Culture Collection Center) No.66882; the strain has relatively strong phosphate solubilizing and IAA (indoleacetic acid) producing capacities and cadmium tolerance, can obviously promote the growth of host plants, increase the chlorophyll content of the host plants and relieve the harm of cadmium pollution to crops under the stress of cadmium when being inoculated with HLC15, and is of great significance in the growth and safe production of the crops in cadmium-polluted soil.

The invention discloses Irpex lacteus YYY-16, and the preservation number of the Irpex lacteus YYY-16 is GDMCC No: 67140. The invention further discloses a terpenoid and a preparation method thereof. The terpenoid has a structure shown in a formula I or a formula II. The invention further discloses application of the terpenoid in preparing tobacco pathogenic bacterium resisting drugs.

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.

The invention provides a rabbit manure organic fertilizer and a preparation method thereof, and the rabbit manure organic fertilizer is prepared by using rabbit manure, rapeseed meal and wheat straw as raw materials through fermentation with a complex microbial inoculant; wherein the complex microbial inoculant is prepared by mixing a phanerochaete chrysosporium bacterial liquid, a bacillus subtilis bacterial liquid and a Thermus thermophilus bacterial liquid. Furthermore, the rabbit manure organic fertilizer is prepared by fermenting the complex microbial inoculant provided by the invention, the degradation of cellulose substances can be remarkably promoted, and the prepared fertilizer has a relatively high seed germination index when being used for germinating seeds. In addition, after fermentation, the content of copper and zinc in a water-soluble state and a weak acid extraction state in the raw materials is reduced, the risk that plants absorb heavy metals is reduced, and the application safety of the organic fertilizer is improved.

The invention relates to a microbial agent containing phanerochaete chrysosporium and ochrobactrum intermedium and a method for degrading organochlorine pesticides. The microbial agent comprises the phanerochaete chrysosporium and the ochrobactrum intermedium. The phanerochaete chrysosporium is classified and named as Phanerochaete chrysosporium, and the preservation number of the phanerochaete chrysosporium is CGMCC (China General Microbiological Culture Collection Center) No. 41170. The classification name of the ochrobactrum intermedia is Ochrobactrum intermedia, and the preservation number of the ochrobactrum intermedia is CGMCC (China General Microbiological Culture Collection Center) No. 33645. The microbial agent disclosed by the invention can efficiently degrade organochlorine pesticides.

The application discloses a kind of for decomposing forest litter composite microbial inoculant, preparation method and its application method, it is related to litter decomposition technical field.The application includes a kind of for decomposing forest litter composite microbial inoculant preparation method, comprising the following steps: S1.to Phanerochaete chrysosporium, Trichoderma reesei and ropegillus catenulatum are respectively carried out directional metabolism preactivation;S2.the three preactivation wet bacteria bodies obtained in step S1 are mixed according to the fresh weight ratio of Phanerochaete chrysosporium:Trichoderma reesei:ropegillus catenulatum 3:2:1, obtain mixed bacteria body;S3.mixed bacteria body is mixed with the straw carrier that is preprocessed by steam explosion according to the mass ratio of bacteria body fresh weight:carrier dry weight 1:2, under the condition of temperature 28 DEG C, relative humidity 92~95%, stationary culture 72 hours, carry out phased micro-perturbation aeration, construct physical symbiosis;S4.the material obtained in step S3 is dried to 15% moisture content at 40 DEG C, obtain composite microbial inoculant;Efficient and stable decomposition of pine forest litter is realized.

The utility model relates to the field of tremella aurantialba production, in particular to a soaking pool raw material circulating device for tremella aurantialba production, which comprises a bottom plate, support legs arranged at the lower end of the bottom plate, a soaking pool body arranged at the upper end of the bottom plate, a vertical column fixedly connected to the upper end of the bottom plate, a top plate arranged at the upper end of the vertical column, and a transverse plate arranged in the bottom plate. A lifting module is arranged at the upper end of the transverse plate, a servo motor is arranged at the upper end of the top plate and drives the lifting module to work, a lifting plate is arranged in the lifting module, a through groove is formed in the lifting plate in a penetrating mode, two first bearing seats are arranged at the upper end of the lifting plate, and a rotating shaft is rotationally connected into each first bearing seat. A rotary motor is arranged at the upper end of the lifting plate; the first gear and the second gear are in transmission linkage through the synchronous belt, the toothed plate is meshed with the first gear and the second gear to achieve horizontal reciprocating circulation movement, the stirring paddle evenly stirs raw materials in the soaking pool body, and production efficiency and product quality are improved.

The present application relates to a kind of active sludge treatment dichlorophenol wastewater method, it is related to wastewater treatment technical field.The present application includes the following steps: (1) domestication of active sludge: adding bacterial body to active sludge, obtain mixed active sludge;Afterwards, mixed active sludge is added to domestication culture solution, domestication is obtained, and domesticated active sludge is obtained;(2) the treatment of dichlorophenol wastewater: domesticated active sludge is mixed with the dichlorophenol wastewater to be treated, and the treatment of dichlorophenol wastewater is realized;Wherein, the bacterial body used is the mixed bacteria of yellow phanerochaete chrysosporium and corynebacterium glutamicum.The present application can realize the efficient degradation of dichlorophenol, further guarantee the degradation effect of TOC in wastewater, the degradation product is safe, and it has the significant advantage of low energy consumption.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The invention discloses a siderophore-mediated symbiotic degradation bacterial agent for remediation of petroleum hydrocarbon contaminated soil. The microbial agent is formed by compounding a high-yield siderophore bacterium pseudomonas aeruginosa DG-1 with the strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No.28382 and a fungus white rot fungus BNCC336258 according to the volume ratio of 1: (2 to 4), and through the siderophore-mediated bacterium-fungus synergistic metabolism effect, the remediation efficiency of the petroleum hydrocarbon contaminated soil under the iron limiting condition (the free iron concentration is smaller than or equal to 1.0 * 10 <-18 > M) is improved. The microbial agent is suitable for treating low-concentration polluted soil with the petroleum hydrocarbon concentration of 0.5-5 wt%, and the total petroleum hydrocarbon removal rate after remediation can reach 80% or above.

The invention discloses a culture method for inducing mycelia to secrete specific extracellular polymeric substances and application of the culture method in sand stabilization, and relates to the field of biotechnology and environmental materials.The culture method comprises the following steps that phanerochaete chrysosporium is inoculated into a liquid fermentation culture medium, and fermentation is conducted under the conditions that the pH ranges from 4.5 to 5.5, the temperature ranges from 35 DEG C to 37 DEG C and shaking culture is conducted; the liquid fermentation culture medium contains tween-80 with the concentration of 0.5-4 g / L and hydrogen peroxide with the concentration of 0.05-0.15 mM as an inducer combination; after the fermentation is finished, separating to obtain fermentation supernate, namely the functional extracellular polymeric substance fermentation liquor; according to the culture method for inducing the mycelium to secrete the specific extracellular polymeric substance and the application of the culture method in sand stabilization, the functional EPS is obtained from the source of regulating and controlling microbial metabolism, and the limitation that a traditional mycelium material only depends on the physical form is broken through.

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for wound healing, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and composite biological bacteria, and carrying out enzymolysis to obtain an enzymolysis product; the composite biological bacterium is a mixture of phanerochaete chrysosporium and streptomyces griseus; carrying out gradient alcohol extraction on the enzymolysis product, merging alcohol extracts, extracting with petroleum ether, and concentrating an organic phase to obtain a component 1; the component 1 is adsorbed by macroporous adsorption resin, the resin is washed with deionized water and then eluted with an eluent, 5-35 min eluent is collected, combined and dried, and the component 1 is obtained. The elution phase is a mixture of cyclohexane, ethyl acetate and acetonitrile. The loropetalum chinense extract is prepared through a specific preparation method, and the wound healing effect is remarkably improved.

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.