29 results about "Chrysosporium" patented technology

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses a tremella auramtialba cultivation and planting method and a closed square cabin special for cultivation and planting, and relates to the technical field of planting. According to the tremella aurantialba cultivation and planting method and the closed square cabin special for cultivation and planting, a cabin body, a rotary cultivation mechanism and a supply mechanism are included, the rotary cultivation mechanism comprises a driver and an operation planting assembly, the operation planting assembly comprises a conveying belt, and the surface of the conveying belt is fixedly connected with a semicircular strip-shaped supporting piece; the top end of the semicircular strip-shaped supporting piece is fixedly connected with a planting disc, the supplementing mechanism comprises a lifting adjuster, a liquid storage tank and a spraying assembly, the spraying assembly comprises a first air cylinder, a second air cylinder and a third air cylinder, and the first air cylinder, the second air cylinder and the third air cylinder are sequentially installed at the output end of the lifting adjuster; the telescopic end of the first air cylinder, the telescopic end of the second air cylinder and the telescopic end of the third air cylinder are all fixedly provided with spraying heads, the adjustable purpose is achieved, the height is adjusted according to strain growth, water is supplemented softly, impact is reduced, and safety and reliability are achieved.

The application provides a gene, a primer set and an application for identifying golden ear, and belongs to the technical field of biological detection. The application obtains specific gene fragments of two species contained in the golden ear through large-scale whole genome comparison analysis, designs species-specific primers based on the specific gene fragments, and further realizes identification of the effective species of the golden ear. The identification is extremely crucial in the production process of the effective species of the golden ear, and can maximize the guarantee of the fruiting rate in the production of the golden ear.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention discloses Irpex lacteus YYY-16, and the preservation number of the Irpex lacteus YYY-16 is GDMCC No: 67140. The invention further discloses a terpenoid and a preparation method thereof. The terpenoid has a structure shown in a formula I or a formula II. The invention further discloses application of the terpenoid in preparing tobacco pathogenic bacterium resisting drugs.

The application discloses a kind of for decomposing forest litter composite microbial inoculant, preparation method and its application method, it is related to litter decomposition technical field.The application includes a kind of for decomposing forest litter composite microbial inoculant preparation method, comprising the following steps: S1.to Phanerochaete chrysosporium, Trichoderma reesei and ropegillus catenulatum are respectively carried out directional metabolism preactivation;S2.the three preactivation wet bacteria bodies obtained in step S1 are mixed according to the fresh weight ratio of Phanerochaete chrysosporium:Trichoderma reesei:ropegillus catenulatum 3:2:1, obtain mixed bacteria body;S3.mixed bacteria body is mixed with the straw carrier that is preprocessed by steam explosion according to the mass ratio of bacteria body fresh weight:carrier dry weight 1:2, under the condition of temperature 28 DEG C, relative humidity 92~95%, stationary culture 72 hours, carry out phased micro-perturbation aeration, construct physical symbiosis;S4.the material obtained in step S3 is dried to 15% moisture content at 40 DEG C, obtain composite microbial inoculant;Efficient and stable decomposition of pine forest litter is realized.

The utility model relates to the field of tremella aurantialba production, in particular to a soaking pool raw material circulating device for tremella aurantialba production, which comprises a bottom plate, support legs arranged at the lower end of the bottom plate, a soaking pool body arranged at the upper end of the bottom plate, a vertical column fixedly connected to the upper end of the bottom plate, a top plate arranged at the upper end of the vertical column, and a transverse plate arranged in the bottom plate. A lifting module is arranged at the upper end of the transverse plate, a servo motor is arranged at the upper end of the top plate and drives the lifting module to work, a lifting plate is arranged in the lifting module, a through groove is formed in the lifting plate in a penetrating mode, two first bearing seats are arranged at the upper end of the lifting plate, and a rotating shaft is rotationally connected into each first bearing seat. A rotary motor is arranged at the upper end of the lifting plate; the first gear and the second gear are in transmission linkage through the synchronous belt, the toothed plate is meshed with the first gear and the second gear to achieve horizontal reciprocating circulation movement, the stirring paddle evenly stirs raw materials in the soaking pool body, and production efficiency and product quality are improved.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The invention discloses a siderophore-mediated symbiotic degradation bacterial agent for remediation of petroleum hydrocarbon contaminated soil. The microbial agent is formed by compounding a high-yield siderophore bacterium pseudomonas aeruginosa DG-1 with the strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No.28382 and a fungus white rot fungus BNCC336258 according to the volume ratio of 1: (2 to 4), and through the siderophore-mediated bacterium-fungus synergistic metabolism effect, the remediation efficiency of the petroleum hydrocarbon contaminated soil under the iron limiting condition (the free iron concentration is smaller than or equal to 1.0 * 10 <-18 > M) is improved. The microbial agent is suitable for treating low-concentration polluted soil with the petroleum hydrocarbon concentration of 0.5-5 wt%, and the total petroleum hydrocarbon removal rate after remediation can reach 80% or above.

The invention discloses a culture method for inducing mycelia to secrete specific extracellular polymeric substances and application of the culture method in sand stabilization, and relates to the field of biotechnology and environmental materials.The culture method comprises the following steps that phanerochaete chrysosporium is inoculated into a liquid fermentation culture medium, and fermentation is conducted under the conditions that the pH ranges from 4.5 to 5.5, the temperature ranges from 35 DEG C to 37 DEG C and shaking culture is conducted; the liquid fermentation culture medium contains tween-80 with the concentration of 0.5-4 g / L and hydrogen peroxide with the concentration of 0.05-0.15 mM as an inducer combination; after the fermentation is finished, separating to obtain fermentation supernate, namely the functional extracellular polymeric substance fermentation liquor; according to the culture method for inducing the mycelium to secrete the specific extracellular polymeric substance and the application of the culture method in sand stabilization, the functional EPS is obtained from the source of regulating and controlling microbial metabolism, and the limitation that a traditional mycelium material only depends on the physical form is broken through.

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for wound healing, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and composite biological bacteria, and carrying out enzymolysis to obtain an enzymolysis product; the composite biological bacterium is a mixture of phanerochaete chrysosporium and streptomyces griseus; carrying out gradient alcohol extraction on the enzymolysis product, merging alcohol extracts, extracting with petroleum ether, and concentrating an organic phase to obtain a component 1; the component 1 is adsorbed by macroporous adsorption resin, the resin is washed with deionized water and then eluted with an eluent, 5-35 min eluent is collected, combined and dried, and the component 1 is obtained. The elution phase is a mixture of cyclohexane, ethyl acetate and acetonitrile. The loropetalum chinense extract is prepared through a specific preparation method, and the wound healing effect is remarkably improved.

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.

This invention provides a solid-state fermentation inoculant, its preparation method, and its application. The preparation method of the solid-state fermentation inoculant involves inoculating *Phanerochaete chrysosporium* into a seed carrier and culturing it in a sterile, pure oxygen environment. Under pure oxygen conditions, the inoculated *Phanerochaete chrysosporium* exhibits a significantly increased reproduction rate, forming a high-density microbial community. Simultaneously, the continuous and stable nutrient supply from the seed carrier enables the inoculant to quickly establish a competitive advantage upon entering materials containing other microorganisms. By seizing nutrients and oxygen resources, it inhibits the growth and reproduction of other microorganisms, ultimately increasing the abundance of white-rot fungi in the fermented product, thereby enhancing the degradation efficiency of petroleum hydrocarbons in the soil. Compared to traditional solid-state fermentation methods, the inoculant production process is very simple and low-cost. The solid-state fermentation inoculant product of this invention has a high microbial density and a relatively large number of target strains, resulting in higher efficiency in soil pollution remediation and increased soil fertility.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant, a preparation method and application. The preparation method comprises the following steps: selecting an anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and a phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776, inoculating the anaerobic bacillus tubingensis YZ-438 (C4) strain with the preservation number of CDMCC66915 and the phanerochaete chrysosporium YZ-460 strain with the preservation number of GMMCC5.776 into a carrier, and mixing and compounding to obtain the composite microbial inoculant, so that the composite microbial inoculant has good air permeability and flexibility due to the pore structure of the carrier buckwheat hull; the mass transfer efficiency and the adsorption effect of oxygen molecules can be improved through synergistic interaction; when the complex microbial inoculant is applied to degradation of compost and agricultural and forestry waste, the enzyme activity and high temperature resistance of the compost and the agricultural and forestry waste can be effectively improved, synergistic complementation on degradation of lignin, cellulose and hemicellulose is achieved, and the degradation efficiency of the lignin, the cellulose and the hemicellulose is high. In the mixing application of vinasse and straw, the temperature can be rapidly increased, and the temperature can be kept at 60-80 DEG C or above for 2 weeks.

The invention relates to the technical field of biology and food, and discloses a self-adaptive regulation and control coffee eurotium cristatum natural eurotium cristatum growing method which comprises the following steps: soaking green coffee beans, removing silver skins on the surfaces of the green coffee beans, and performing high-pressure sterilization treatment; inoculating the sterilized coffee beans with a microbial inoculum containing aspergillus cristatus strains for solid state fermentation; baking the fermented coffee beans to obtain finished coffee beans; the Latin literature name of the aspergillus cristatus is Aspergillus cristatus, and the strain number of the aspergillus cristatus is GDMCC 67444. According to the method, the green coffee beans are subjected to single-bacterium / mixed-bacterium fermentation treatment through specific strains, the edible safety and functionality of the green coffee beans are improved, core sensory quality such as acidity and fragrance of coffee is directionally improved, compared with unfermented green coffee beans, the quality advantage is remarkable, the fermentation process is stable and controllable, the actual production requirements of the coffee processing industry are met, and the method is suitable for popularization and application. The method has outstanding practical value and popularization prospect.

The application discloses a white-cyst Irpex lacteus and application of the white-cyst Irpex lacteus in degradation of corn stalks and fermentation production of erucic acid amide. The white-cyst Irpex lacteus is classified and named as Irpex lacteus J2, and has been preserved in the China Center for Type Culture Collection on November 24, 2023, with a preservation number of M20232318. The strain has a complete lignocellulose-degrading enzyme system, and can produce erucic acid amide by taking corn stalks as the only carbon source.

The invention provides a biomass charcoal adsorption material and a preparation method thereof, and belongs to the field of pesticide degradation. The preparation method comprises the following steps: preparing biochar, preparing modified biochar and compounding. The compounding method comprises the following steps: adding modified biochar, bacillus subtilis, pseudomonas FH2, bacillus licheniformis and phanerochaete chrysosporium into deionized water, stirring, filtering, adding a filter cake into a chitosan solution, then adding Tween 60, stirring for 2-3 hours at the speed of 500-600r / min, slowly dropwise adding a glutaraldehyde solution, continuously stirring for 8-10 hours after dropwise adding, finally centrifuging at the speed of 7000-8000r / min, and drying to obtain the biochar composite material. Collecting solids, and drying to obtain a biomass charcoal adsorption material; the biomass charcoal adsorption material disclosed by the invention has broad spectrum on pesticide degradation, the degradation rate on trichlorfon reaches 87 to 89 percent, the degradation rate on acetamiprid reaches 89 to 93 percent, the degradation rate on deltamethrin reaches 93 to 96 percent, and the degradation rate on DDT reaches 65 to 70 percent.

The present application belongs to the technical field of waste conversion fertilizer, and provides a kind of compound organic fertilizer and its preparation method, the method steps are as follows: S1, by weight, rice straw 27-30 parts, bran 9-10 parts and animal manure 8-9 parts are mixed to obtain initial raw material;S2, 0.85-0.9 parts of oxidized modified straw biochar and 0.5-0.55 parts of ammoniated superphosphate are added into the initial raw material at the same time and mixed, and then the moisture content is adjusted to 53±3%, to obtain the base material;S3, add phanerochaete chrysosporium suspension and bacillus subtilis activation solution to the base material and mix evenly to obtain fermentation material;S4, the fermentation material is composted and fermented for more than 28 days to obtain the compound organic fertilizer.The present application shortens the composting time to improve the preparation efficiency of organic fertilizer, and further reduces the phytotoxicity of the prepared organic fertilizer.

The application provides a chrysosporium inosporum breeding method and relates to the technical field of edible mushroom cultivation. The breeding method is as follows: the chrysosporium fruiting body is hung and cultured, and after the spores in the fruiting body are separated out, the spores are moved to a flat plate culture medium to culture the chrysosporium single colony, two chrysosporium single colonies are randomly picked and cross lines are drawn on the same clean flat plate culture medium to carry out hybridization culture, and the liquid strain of phanerochaete chrysosporium is simultaneously introduced as a mycelium growth auxiliary, is evenly smeared on the culture medium, and the ear is induced. The breeding method solves the technical problems of the existing cultivation method, such as complex operation, tedious steps, high requirement for equipment and long cultivation time, and realizes the technical effects of fast spore culture speed, simple operation, low equipment requirement and meeting the needs of factory production.

The invention discloses a selenium source regulation and control method for directionally producing high-value selenium-rich phellinus igniarius mycelium or sporocarp, and belongs to the technical field of edible and medicinal fungus cultivation and microbial fermentation. The invention provides a target-oriented differential selenium source strategy aiming at the technical contradiction that a single selenium source cannot meet the requirements of different growth stages of phellinus igniarius in the prior art. When the phellinus igniarius mycelium is used as a target product, a fermentation strategy of adding selenium-enriched yeast in the whole process is adopted; when phellinus igniarius sporocarp is used as a target product, a staged strategy is adopted: selenium-enriched yeast is added in a mycelium culture stage, and sodium selenite is added in a sporocarp culture stage. According to the method, the rule that the mycelium stage is adaptive to organic selenium and the sporocarp stage is adaptive to inorganic selenium is defined for the first time, and through accurate stage-selenium source matching, the selenium content, yield and comprehensive quality of the mycelium or the sporocarp are synchronously improved successfully on the premise that mycelium growth is hardly inhibited.

The invention discloses a method for preparing a humic acid fertilizer synergist based on biological-chemical activated weathered coal, and relates to the technical field of humic acid fertilizer synergists. The method comprises the following steps: mixing bacillus subtilis, trichoderma harzianum and phanerochaete chrysosporium stock seeds to obtain a complex microbial inoculant, and activating the weathered coal by using the complex microbial inoculant to obtain biologically activated weathered coal; mixing the biologically activated weathered coal with potassium hydroxide and paper pulp lignin liquid, and performing chemical solid-phase activation under a heating condition to obtain an activated product, namely the humic acid fertilizer synergist. Through synergism of biological activation and chemical activation, the content of humic acid, organic matters and fulvic acid in the humic acid fertilizer synergist can be obviously increased, so that growth and development of rape are facilitated. Moreover, the weathered coal is activated by adopting the paper pulp lignin liquid, and meanwhile, the resource utilization of the paper pulp lignin liquid and the high-order conversion of the weathered coal are realized, so that the method has great practical significance.

The application discloses a chrysosporium strain ZJJE004 and a factory cultivation method thereof, the chrysosporium strain ZJJE004 ( Naematelia aurantialba ), is preserved in the China General Microbiological Culture Collection Center, and the preservation date is May 12, 2022, and the preservation number is CGMCC NO.40189, and the ITS sequence is shown as SEQ NO.1. The factory cultivation method of the chrysosporium strain ZJJE004 adopts a liquid strain one-time inoculation method, does not need to inoculate tissue blocks, greatly optimizes the cultivation method, and is simple in cultivation method, fast in mycelium growth, low in pollution, strong in mycelium, obviously shortens the cultivation period of the chrysosporium, and can be picked in 45-55 days; the ear yield is high, the size of the fruiting body is uniform, the mushroom effect is good, the conversion rate of the first crop can be higher than 80%, the yield is high, large-scale cultivation can be realized, has high application value, and is suitable for factory production.

The application relates to the technical field of agricultural solid waste resource utilization, and discloses a composite microbial inoculum for fertilizer preparation of multi-source agricultural and forestry organic waste and a preparation method thereof, which comprises the following steps: the composite microbial inoculum is prepared from D-anhydrous glucose, an induced mesoporous carrier dispersion liquid, veratryl alcohol, urea, a phanerochaete chrysosporium seed liquid, a trichoderma reesei seed liquid and a bacillus licheniformis seed liquid. Two-stage time sequence fermentation is adopted during preparation, fungi are inoculated and cultured first, then bacteria are inoculated and urea is added. Ammonia produced by bacterial catalysis is used to drive the spontaneous increase of the pH of a fermentation system, trigger the in-situ crystallization of precursor ions, and build a multi-level layered coating configuration with a porous diatomite skeleton in the inside, an alkali lignin planting layer in the middle layer, and an ammonium magnesium phosphate crystal armor layer in the outer layer. The application effectively blocks interspecific competition during co-culture, endows the microbial inoculum and degradation enzyme system with excellent mechanical shear resistance, can provide long-acting acid-alkali buffering and precise nutrient release in the complex environment of composting, and improves the waste degradation efficiency.

The invention relates to the technical field of cigarette perfuming, in particular to an aroma-producing strain CXM-7 and application thereof, a fermentation product and a preparation method and application of the fermentation product. The aroma-producing strain CXM-7 is Neurospora terasperma, and the preservation number of the aroma-producing strain CXM-7 is CGMCC (China General Microbiological Culture Collection Center) No. 41928. The aroma-producing strain CXM-7 has the characteristics of aroma production, nicotine resistance and high biological activity. The strain can be fermented by taking waste tobacco powder as a culture medium, and the obtained fermentation product contains a plurality of typical aroma substances for improving the tobacco aroma, such as linalool, geranyl acetone, 4, 7, 9-megastigmatriene-3-ketone, scopoletin, dihydroactinidiolide, gamma-butyrolactone, ethyl stearate and other aroma components with faint scent, sweet aroma and fruity aroma; the variety and content of aroma components in the fermentation liquor are high, and the fermentation liquor is endowed with sweet aroma and faint scent.

The invention relates to the technical field of environmental microorganisms, and discloses a method for directionally synthesizing high-activity humus based on a microorganism co-culture system.The method comprises the steps that firstly, a co-culture flora composed of phanerochaete chrysosporium, bacillus subtilis and agrobacterium tumefaciens according to a specific proportion is constructed; and then inoculating into the pretreated agricultural wastes, and carrying out two-stage temperature-controlled fermentation: in the first stage, carrying out low-temperature aerobic promotion on hydrolysis to produce acid, and in the second stage, carrying out heating micro-aerobic drive on oxidative polymerization. Through the orientation process, the lignocellulose raw material can be efficiently converted into high-activity humus within 12 days. Through the synergistic effect of functional flora and accurate control of physical conditions, directional rapid synthesis of humus is achieved, the total content of humus in the obtained product is high (> = 25%), the ratio of humus to humus is appropriate (1.5-2.0), the biological activity is high (the seed germination index is > = 120%) and is far better than that of a traditional compost product, and a new technology is provided for high-value utilization of agricultural waste and soil improvement.

The invention belongs to the technical field of agricultural resources and environment, and particularly relates to a soil remediation fertilizer prepared from agricultural wastes and a preparation method of the soil remediation fertilizer. The method comprises the following steps: mixing mixed straws, livestock and poultry manure, soybean meal and wheat bran according to a specific ratio, and then carrying out three-stage microbial fermentation: firstly, inoculating trichoderma reesei and bacillus subtilis for primary degradation; then, phanerochaete chrysosporium and pseudomonas are inoculated, a sesamol inducer is added, and humus is synthesized in an oriented mode; and finally, inoculating a functional bacterial agent consisting of phosphate-solubilizing bacteria, potassium-solubilizing bacteria and nitrogen-fixing bacteria. According to the invention, through microbial agent relay and molecular induction, efficient conversion of wastes is realized, and the obtained fertilizer can significantly improve soil structure, improve humus activity, activate microflora, and synchronously enhance physical, chemical and biological fertility of soil.

The invention belongs to the technical field of agricultural planting, and particularly relates to a straw returning method capable of achieving greenhouse gas emission reduction. According to the specific technical scheme, the straw decomposing microbial agent comprises microbial preparations, and the microbial preparations comprise bacillus subtilis, trichoderma harzianum and phanerochaete chrysosporium. When the straw decomposing microbial inoculum is used for composting, the temperature can be quickly increased, the composting efficiency is improved, lignin in the straw can be degraded to 60% or above after composting is carried out for 20 days, and harmful microorganisms and weed seeds in the straw can be effectively killed. By using the microbial agent disclosed by the invention, short-term and in-situ rapid composting can be realized; and the compost can be quickly returned to the field in situ. The yield of crops is guaranteed, and meanwhile the emission amount of greenhouse gas is effectively reduced.

This invention discloses a siderophore-mediated symbiotic degradation agent for the remediation of petroleum hydrocarbon-contaminated soil. This agent is composed of *Pseudomonas aeruginosa*, a high-side-producing bacterium. Pseudomonas aeruginosa DG-1, strain preservation number CGMCC No. 28382) and the fungus white rot fungus ( Phanerochaete chrysosporium BNCC336258 is formulated in a volume ratio of 1:(2-4) and enhances iron-limiting conditions (free iron concentration ≤1.0×10⁻⁶) through siderophore-mediated bacterial-fungal synergistic metabolism. ‑18 The remediation efficiency of this microbial agent on petroleum hydrocarbon-contaminated soil (M) was demonstrated. This agent is suitable for treating low-concentration contaminated soil with petroleum hydrocarbon concentrations of 0.5-5 wt%, achieving a total petroleum hydrocarbon removal rate of over 80% after remediation.

The invention relates to the field of soil remediation, and discloses a microbial agent directional remediation method for heavy metal contaminated soil, which comprises the following steps: firstly, preparing a microbial agent from bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, humid cellulomonas and bacillus amyloliquefaciens; the microbial agent is applied to the heavy metal contaminated soil in a manner of uniformly spreading water for flushing, and the remediated soil is obtained. Raking the remediated soil, adjusting the water-holding capacity of the remediated soil, applying a base fertilizer, and irrigating the remediated soil; rice seedlings are transplanted, duckweed is placed, flooding management is conducted till rice in the rice field is mature, and the soil remediation process is completed; the problems that in the prior art, when a passivator is used for repairing alkaline soil, the function is single, the soil repairing effect is weak, the maintenance time of the repaired soil is not long, and consequently repairing needs to be conducted repeatedly are solved.