Composite microbial agent for river treatment and preparation method and application thereof

A technology of composite microbial agent and microbial agent, which is applied in the field of environmental microorganisms, can solve the problems of unsatisfactory application effect of microbial comprehensive restoration technology, difficulty in ensuring the effect of water pollution control, mutual restraint, etc.

Inactive Publication Date: 2017-08-15
XIANGTAN ZHILIAN TECH MATASTASIS PROMOTE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main practice at present is to add compound microbial bacterial agents in polluted water at one time. These existing technologies still have the following problems: 1) If the types and quantities of bacteria added are too small, due to the complexity of the types of water pollution sources in my country , it is difficult to guarantee the effect of water pollution control; and if there are too many types and quantities of bacteria, there may be competition or even mutual restraint among the bacteria, which will also affect the effect of pollution control
In addition, the microorganisms released in large numbers will cause secondary pollution to the water if they are not treated in time; 2) The composite microbial agents used are mainly to degrade organic pollutants. Along with various solid suspended particles, colloidal particles, etc., the removal of such pollutants is mainly by physical or chemical methods at present. Although there are also reports on microbial flocculants, there are very few types of high-efficiency microbial flocculant-producing bacteria that have been screened so far. The vast majority remain in the laboratory stage
Therefore, there is still a lack of comprehensive and efficient strains and mature repair technology, resulting in unsatisfactory application of microbial comprehensive repair technology in industry

Method used

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  • Composite microbial agent for river treatment and preparation method and application thereof
  • Composite microbial agent for river treatment and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of composite microbial agent for in-situ remediation of urban and rural river sewage

[0039] (1) Preparation of composite microbial bacterial agent A:

[0040] (1.1) Take the seeds of Bacillus megaterium CCTCC No.M2012352, inoculate them in solid slant medium I for activation culture, the activation conditions are: 32°C, activate the culture for 48h; then inoculate the activated Bacillus megaterium CCTCC No.M2012352 into liquid culture The base I was expanded and cultivated, and the expanded culture conditions were as follows: in a rotary shaker at 200 rpm, cultivated at 30°C for 48 hours; the expanded cultured Bacillus megaterium CCTCC No.M2012352 was inoculated in the fermentation medium I by 10% of the volume of the fermentation broth , under the rotation speed of 150rpm in a rotary shaker, after culturing at 32°C for 96h, the concentration of Bacillus megaterium CCTCC No.M2012352 in the obtained fermentation liquid of Bacillus megaterium CCTCC No.M20123...

Embodiment 2

[0067] Application of composite microbial agent for in-situ remediation of urban and rural river sewage

[0068] A certain river in the suburbs of Tongdao County, Huaihua, Hunan Province, has urban domestic sewage, rural breeding and planting sewage discharges, and mountain floods all year round. The river water is turbid and smelly all the year round, and COD, ammonia nitrogen, and total phosphorus exceed the standard. Get 2L water sample from this river course, add 2mL of the liquid compound microbial bacterial agent A that embodiment prepares, add bacterial agent once every three days, after feeding continuously for three weeks, measurement result shows, water body COD is by 260mg / L before putting in microorganism decreased to 14.5mg / L; ammonia nitrogen content decreased from 17.2mg / L before restoration to 0.69mg / L; total nitrogen decreased from 32.4mg / L to 0.93; total phosphorus decreased from 2.80mg / L to 0.27; add 20mL to implement For the liquid microbial agent B prepare...

Embodiment 3

[0072] Application of composite microbial agent for in-situ remediation of urban and rural river sewage

[0073] A certain river in Shimen County, Changde, Hunan, is turbid all year round, and COD, ammonia nitrogen and total phosphorus exceed the standard. Get 2L water samples from the river, add 2.5mL of the liquid composite microbial agent A prepared by the embodiment, add the agent once every four days, after three weeks of continuous feeding, the measurement results show that the COD of the water body is changed from 245mg / L decreased to 12.8mg / L; ammonia nitrogen content decreased from 19.2mg / L before restoration to 0.64mg / L; total nitrogen decreased from 35.24mg / L to 0.97; total phosphorus decreased from 3.03mg / L to 0.28; For the liquid microbial agent B prepared in Example 1, the turbidity and chromaticity of the water quality improved significantly after being added for 3 days, and the transparency increased from 15 cm to 27 cm, and the transparency increased to 68 cm...

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Abstract

The invention discloses a composite microbial agent for river treatment and a preparation method and application thereof. According to the composite microbial agent, an agent A is formed by mixing five fermentation liquids formed by individual cultivation of a bacillus megatherium CCTCC No.M2012352, a bacillus subtillis CGMCC No.1.2162, a bacillus licheniformis CGMCC No.1.6510, a bacillus amyloliquefaciens CGMCC No.1.7463 and a phanerochaete chrysosporium BKM-F1767; and an agent B is a fermentation liquid formed by individual cultivation of a debaryomyces hansenii CGMCC No.5770. The composite microbial agent is capable of degrading amino nitrogen and nitro nitrogen in water, reducing the phosphorus concentration and precipitating solid suspended particles, somatic cells and colloid particles in water.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and relates to a compound microbial bacterial agent for river channel management and its preparation method and application. Background technique [0002] Urban rivers and lakes are water environments that are closely connected with human production and life. With the growth of population and the acceleration of urbanization, a large amount of pollutants are directly discharged into rivers and lakes, resulting in organic matter, ammonia nitrogen, total phosphorus and hydrogen sulfide in the water. Pollutants such as pollutants continue to increase, and the natural purification capacity of the water body is seriously insufficient, seriously restricting the sustainable development of the economy and the environment, and endangering human health. [0003] The traditional means of removing water pollutants mainly include physical and chemical methods. Although they have certain effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C02F3/34C12R1/11C12R1/125C12R1/10C12R1/07C12R1/645
CPCC02F3/34C02F3/347C02F2103/007C12N1/14C12N1/16C12N1/20
Inventor 刘军亮
Owner XIANGTAN ZHILIAN TECH MATASTASIS PROMOTE CO LTD
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