241 results about "Every Three Days" patented technology

The invention provides a lady health-care wine and a preparation method thereof. The health-care wine is prepared from angelica, hemlock parsley, radix paeoniae alba, prepared radix rehmanniae, raw radix rehmanniae, radix ophiopogonis, prepared fleece-flower root, lucid ganoderma, radix astragali, ginseng, rhizoma polygonati, poria with hostwood, polygala root, stir-fried spine date seed, deer fetus paste, antler, donkey-hide gelatin, glue of tortoise plastron, stir-fried eucommia, mistletoe, pawpaw, radix achyranthis bidentatae, broomrape, rhizoma drynariae, spatholobus stem, cassia bark, cornus officinalis, epimedium, Curculigo orchioides, saffron, Cordyceps, rhizoma cyperi, agilawood, Flos Caryophyllata, dalbergia wood, Turmeric Root-tuber, Rhizoma Zedoariae, cowherb seed, pseudo-ginseng, raw pollen typhae, pummelo pee, fingered citron, citron, raw rhubarb, fructus cannabis, bush-cherry seed, peach kernel, Chinese radish seed, semolina seed, cassia seed, Plantain seed, dodder, wolfberry fruit, raspberry, schisandra fruit, cherokee rose, Semen Euryales, gynostemma pentaphyllum, raw hawthorn, red rice, gastrodia tuber, kudzu root, chrysanthemum flower, calcined oyster and dragon bone, white spirit and yellow wine. The preparation method comprises the following steps: soaking the Chinese medicinal herbs in 50 vol% top-grade white spirit for 36 days (stirring every three days),and discarding the Chinese medicinal herbs after 36 days; and adding top-grade yellow wine (twice of white spirit), adding a right amount of honey (according to mouthfeel), sealing, and soaking for 36 days (stirring every three days) to obtain the lady health-care wine. When people drink 50ml of the health-care wine a day, the health-care wine can tonify the liver and kidney, recuperate yin and yang, invigorate qi, strengthen the bone, nourish the blood and refresh the brain.

The invention relates to a method for amplifying cytokine induced kill (CIK) cells and a CIK cell preparation, which belong to the field of in-vitro culture of immune cells. The method concretely adopts the following procedures that: a, lymphocyte cell separation liquid is used for separating out peripheral blood mononuclear cells (PBMC), a culture bag is covered by CD3mAb and CD137mAb in advance, the concentration of the PBMC obtained through separation is regulated to 1*10<6> / ml by a serum-free culture medium, in addition, IFN-gamma is added to obtain the final concentration being 1000 mu / ml, and the materials are transferred to the culture bag to be cultured; b, CD3mAb, CD28mAb and CD137mAb are added after the culture for 24h, in addition, the prepared serum-free culture medium is added, IL-1alpha, IL-2, IL-12 and IL-15 are added into the prepared serum-free culture medium, and obtained CIK cells are collected through centrifugation after the continuous culture for 7 to 21 days; and c, in the culture process of the step b, the cells in the culture bag are counted every three days, in addition, the culture medium is supplemented according to the concentration of the cells, and the CD3mAb, the CD28mAb and the CD137mAb are added to the corresponding concentration every six days, so the CIK cell generative cell times and the cytotoxin activeness are improved.

The invention provides a processing method of pu-er tea with inoculated fungi microorganism. During the first day of pu-er tea pile-fermentation, aspergillus niger red koji and penicillum sp. red koji is mixed together by the ratio of 1-2:1 and is added into the production process of pu-er tea by the weight percentage of uniform mixed red koji and basic tea of 1:125-250; during the second day of pu-er tea pile-fermentation, saccharomyces sp. is added into the production process of pu-er tea by the weight percentage of saccharomyces sp. and basic tea of 1:250-500 and then material is gathered together and covered with fermentation cloth; fermentation gathering temperature is controlled between 40 celsius degree and 65 celsius degree and gathering overturning has to be carried on apporiately to control moisture content between 14 percent and 20 percent; when tea leaves moisture content is over than 20 percent, ditch recanalization has to be carried on every day; when tea leaves moisture content is 14 percent to 20 percent, ditch recanalization has to be carried on every three days; with shortened processing time, rich water extract, proper tea polyphenol and amino acids is retained and a certain amount of caffeine, hydration pectin, theaflavins and theabrownin is added; compared with natural pile-fermentation, the quality of the invention is superior and more stable and microbial indicator is equal to that of red tea and green tea.

The invention relates to a cultivating method of a sinocyclocheilus grahami seedling, belonging to the technical field of fish culture. The cultivating method comprises the steps of the cultivation of fish fries growing mouths in rich water, artificial cultivation of fish fries, the rearing of fish fries at middle and rear stages and pest control. In the cultivation of fish fries growing mouths in rich water, a temporarily-cultivating pond is arranged in a tunnel, bait for fish fries growing mouths is chlamydomonas and rotatoria cultivated in rich water and the fish fries are cultivated at water temperature of 15 DEG C for 10-12 days. In the artificial cultivation of fish fries, the fish fries are cultivated by eel powder feed with the protein content of 40 percent for 40 days. The feed in the fish fries at middle and rear stages is carp crushed feed with the protein content of 39 percent. In the pest control, the water quality and the conditions of fish bodies are detected every three days, 50-80ml / mu of povidone iodine for controlling bacterial infection and 50-50ml / mu of rotatoria one-time removal (ammonium ethylene-bis-dithiocarbamate) for controlling balantidiasis are splashed every 15 days. The invention can effectively improve the survival rate and the growth speed of inocyclocheilus grahami seedlings, is simple and convenient, and is beneficial to being popularized and generalized. Bacterial.

The invention belongs to the field of cell biology and relates to a method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells. The method comprises that HUCMSCs and Schwann cells are putted in Transwell with apertures of 0.4 microns to be co-cultured separately; a Schwann cell culture medium is utilized as a cell culture medium and half or all of the cell culture medium is replaced every three days; and then neural cells can be obtained after two weeks of culturing. Through a separate co-culturing method utilized by the invention, an umbilical cord can be digested by a mixture of composite collagenase NB4 and hyaluronidase and thus a large amount of HUCMSCs can be separated. High purity HUCMSCs can be obtained in third generation passage cells. In the invention, HUCMSCs are induced and differentiate into neural cells, wherein a cell differentiation rate is over 70%. Expressing rate ranges of specific markers, such as NF-200, nestin, Betar-III-tubulin, etc., of neural cells are from 70 to 80%.

The invention discloses a planting method for dendrobium officinale. The method comprises the following steps: crushing raw materials such as peat soil, peanut shells, pine wood bran, pine barks and a small amount of bat manure to obtain a substrate, performing high-temperature treatment, sterilizing and sowing dendrobium seeds, and transplanting the dendrobium to a greenhouse after a year; controlling proper temperature and moisture, wherein a spraying manner is adopted, the watering frequency is once every three days in January to March, once every two days in March to June, once every 5 to 6 days in June to September and once every three days in September to December; keeping the temperature in the greenhouse be 18 to 22 DEG C in the daytime and 15 to 20 DEG C at night; preventing the soft rot and the black spots of dendrobium officinale, and preventing and controlling snails, sarcoptes mites, phenacaspis dendrobii kuwana and red spider mites. According to the planting method for dendrobium officinale, the substrate with high adaptability to dendrobium officinale is adopted, and high dendrobium officinale transplantation survival rate, high growth speed, high yield and high active ingredient content are achieved.

The invention provides a man health-care wine and a preparation method thereof. The health-care wine is prepared from the following components: cartialgenous, boschniakia rossica, cistanche, cynomorium songaricum, herba epimedii, hippocampi, testiset penis phocae, gecko, raw rehmannia, fructus corni, semen cuscutae, mulberry seeds, raspberry, schisandra chinensis, fruit of Chinese wolfberry, fructus cnidii, plantain seeds, lucid ganoderma, Jilin Ginseng, poria, cordyceps sinensis, rhodiola rosea, saussurea involucrate, deer penis, bullwhack, dog penis, bombyx mori, chilopod, angelica, radix paeoniae alba, radix glycyrrhizae preparata,black ant, red ant, fried Chinese date, prepared polygonum multiflorum thumb, white wine and yellow wine. The method comprises the following steps: taking white wine over 50 degrees, and pouring the wine until covering the medicinal materials, sealing, soaking for 36 days, stirring one time every three days; discarding the drug packaged by the gauze; adding the best-quality yellow wine the weight of which is two times of the white wine; adding a proper amount of honey according to the taste, sealing and soaking for 36 days, and stirring one time everythree days. The man health-care wine has the effects of tonifying kidney and cultivating yuan, strengthening du and softening blood vessel, replenishing qi and invigorating spleen, and nourishing blood and regulating liver after 50ml of prepared wine is taken every day.

The present invention discloses the application of RE element in cultivating aerobic sludge particle and controlling sludge expansion. During cultivating aerobic sludge particle, RE element solution of concentration below 30 mg / L is thrown into the biochemical reactor once every three days. Thus cultivated aerobic sludge particle with RE element has short forming period, large particle, low water content, high deposition rate, low SVI, high COD load and other advantages.

Disclosed are multifunctional soil maturing agent and a preparation method thereof. The soil maturing agent comprises biochar and poultry excrement in a weight ratio of 10:3-10:5. The preparation method includes: mixing the biochar and the poultry excrement proportionally, well mixing, and adjusting water content to about 60%; and covering the mixture with a thin layer of biochar, turning compost each day 20 days before composting, keeping the water content of 60% unchanged, turning the compost once every three days from the twentieth day to the thirtieth day, turning the compost once a week, and avoiding sunlight and rain. The compost is made of mixture of biochar and farm poultry excrements such as pig excrement. The problems of odor, long formation time and low fertilizer efficiency of traditional compost are solved. The poultry excrements can be also be used fully, and waste is turned into wealth. Composted products can be used as oil conditioner / maturing agent and gardening medium. The multifunctional soil maturing agent has promising market prospect.

The invention discloses a method for improving the flowering quality of Christmas begonia, comprising the following steps: selecting the robustly grown Christmas begonia; carrying out short-day treatment on the Christmas begonia two months before flowering continuously for 30 days, wherein, the illumination intensity is between 15000 and 18000 Lx; applying a 1000 times liquid of 12# Peters professional every three days combining watering during the short-day treatment, wherein, the pH is 5.6-6.1, and EC is 1.3-1.5; watering 0.3% monopotassium phosphate for one time every 7 days; and applying a 600 times liquid of 1# Peters professional every five days during other growth periods, wherein, the culturing temperature in the whole growth period is 18-21 DEG C, and the humidity is 70-75%.

The invention relates to a simple method for promoting AP sweetsop flower formation. The simple method for promoting the AP sweetsop flower formation specifically comprises the steps of performing selection, clipping, flower formation promotion, water management and fertilizer application management in a clipping and flower formation promotion period, wherein the clipping and flower formation promotion period ordinarily ranges from the last ten-day period of August of the current year to the first ten-day period of March of the next year; selecting and topping young sprouts grown in the current year, spraying plant growth regulator mixed liquor on topped branches without half of leaves and then performing the spaying for one time every three days, totally performing the spraying for 3-5 time so as to promote axillary bud growth, and providing guarantee for flower bud differentiation. In addition, when new axillary buds grow, 80mg / L-100mg / L auxin (IAA) can be spayed to serve as an auxiliary agent. In the clipping and flower formation promotion process, compound fertilizer, urea, lime and potassium chloride are ordinarily applied in five days before clipping. By utilizing the simple method, the flower formation rate of treated branches is obviously improved, an AP sweetsop flowering phase can be five days ahead of the phase by adopting a traditional flower formation promoting mode, the flower formation rate is improved by higher than 70%, and a basis is provided for efficient sweetsop cultivation.

The invention relates to a method for pickling pork head meat. The reasonable-process method for pickling pork head meat, capable of being used for making the pickling pork head meat with the unique flavor does not exist in the prior art. The method provided by the invention comprises the work procedures of raw material meat processing, pickling, rinsing, baking, cooking, packing and sterilization. In the raw material meat processing work procedure, pork heads are used as raw materials, pork tongues and pork faces are taken down, the pork heads are cut into halves, and the pork brain is taken out to obtain the port cranium; in the pickling work procedure, the pork cranium, the port tongues and the pork faces are pre-cooled to the temperature below 10 DEG C, pepper salt is smeared on the pork cranium, the port tongues and the pork faces, the picking is carried out for 28 to 32 days, the jar turning is carried out every day in the early four days, and the jar turning is carried out every three days from the fifth day; the cooking work procedure is carried out after rinsing and baking, the pork cranium is placed into boiling water to be boiled for 42 to 48 minutes to obtain pork cranium meat, the pork noses, the pork tongues and the pork faces are steamed for 18 to 22 minutes by 100-DEG C steam, and the pork ears are steamed for 28 to 32 minutes by 100-DEG C steam; and the finished products of pickled pork head meat are obtained through the work procedures of packing and sterilization. The method has the advantages that the process is reasonable, and the manufactured pork head meat has unique flavor.

The invention relates to a bougainvillea spectabilis cottage technology, in particular to a bougainvillea spectabilis mud matrix cuttage method. The technical scheme is that flower mud special for fresh flower arrangement is cut into cubes with 2.5 cm edges, bougainvillea spectabilis branches are cut into segments ranging from 10 cm to 15 cm, the base portions of the branches are inserted into the flower mud, a distance from the base ends of the branches to the bottom of the flower mud cubes is 1 / 3 of each edge of the flower mud blocks, the flower mud inserted with the bougainvillea spectabilis branches is erected onto river sand with 2 cm thickness and then is covered with river sand, the particle size of the river sand is smaller than 2 mm, the flower mud is watered completely, one-time watering is performed every three days after complete watering, and when new root visible parts penetrating through the flower mud blocks reach 3 cm, cutting seedlings and the flower mud blocks are wholly taken out to be transplanted. The bougainvillea spectabilis mud matrix cuttage method enables the transplanting survival rate of the bougainvillea spectabilis cutting seedlings to reach 100%.

The invention relates to a preparation method of a trace element water-soluble fertilizer capable of enhancing wetting performance. The method comprises the following steps: taking a humic acid reagent, EM bacteria, water-soluble starch, rice bran, wheat bran and water, fermenting the materials for 15-20 days in a sealing mode at the temperature of 45-65 DEG C, uniformly stirring the materials every three days, and performing constant-temperature drying at the temperature of 75 DEG C to obtain the modified humic acid reagent; taking water and heating water to the temperature of 30-40 DEG C, adding a chelating agent and an anti-oxidant, then adding trace elements, using boric acid and borax to adjust the pH value of a solution to 4.0-5.5, adding the modified humic acid reagent and a silicone surfactant, adding an antifoaming agent and the anti-oxidant, then adding iron sulfate heptahydrate, supplying water, stirring the materials while adding the materials, and after stirring, filteringinsoluble substances to obtain the trace element water-soluble fertilizer capable of enhancing wetting performance. The trace element water-soluble fertilizer capable of enhancing wetting performancehas good moistening performance on plants leaves, and has the advantages of high utilization rate, antioxidation, simple preparation method, low cost, and easy operation.

The cultivation method of sea urchin tetraploid fingerlings has the steps that the urchin seedling is dried in shade for 0 to 40 minutes. The 0.5 to 2.0 ml 0.5 mol / L KCl solution is injected from the sea urchin membrana peristomialis for the spawing and spermiation. The urchine eggs and sperms are collected, and the hybrid fertilization is implemented. The developmental temperature of fertilized eggs is controlled between 17 to 18 DEG C. The fertilized eggs in concentration with a mix silk net are collected after 55 to 65 minutes of the fertilization, which are packed in a closed bag and disposed in a pressure vessel of a hydrostatic pressure equipment and the ambient temperature is identified between 17 to 18 DEG C. The pressure treatment is implemented after 60 to 70 minutes of the fertilization, in which the pressure is controlled between 70 to 80MPa and the processing time is controlled between 6 to 8 minutes. Afterwards the fertilized eggs are disposed inside a bin for hatching and stirred, and the incubation temperature is controlled between 16 to 18 DEG C. After floated, the embryos are selected with the proper aeration. The bait feeding is implemented during the larvae cultivation period. The water is exchanged 1 to 3 times everyday, and each time the quantity of water exchange is 1 / 3 to 2 / 3. The pond water is exchanged once every three days. The tetraploid fingerlings cultivated with the method can produce the triploid fingerlings, thus the time of processing and the time to the market are extended.

The invention discloses houses for breeding silkworms with living folium mori, row clipping cocooning frames and a method and relates to rearing instruments and a new method which is to directly arrange a plurality of houses in a mulberry field, lift the expansion rods (6) according to weather move the houses with silkworms, move the house (11) for breeding silkworms with living folium mori every four days, breed silkworms in the grids of the row clipping cocooning frames (19), which is provided with protrusions (16), clamping grooves (17) and grid layers (18), one by one at regular density and sequence, collect folium mori for silkworms layer by layer and grid by grid and bridge to supply folium mori in turn every three days. The problem of inter-row space for silkworms is solved, the four essential factors including ventilation, cooling and drying, adequate living folium mori and disinfecting for preventing diseases are guaranteed, an economic growth mode is changed, the working time per kilogram of cocoon is reduced to 0.2 hour from 5 hours, which is less than the 1.6 hours in Japan, land productivity and labour productivity are improved considerably, one person can breed 30 boxes of silkworms in a single season, high productivity, high efficiency, high quality, low consumption and labor conservation are achieved, and thus, the houses and the method promote the specialization, intensification, scale-up, industrialization, labor conservation and modernization of the silkworm and mulberry industry.

The invention provides a disease comprehensive prevention and control method for greenhouse-cultured penaeus vannamei. The disease comprehensive prevention and control method for the greenhouse-cultured penaeus vannamei is characterized by comprising the following steps: 1, water body regulation and control: (1), matching one culture pond with one disinfection tank, and disinfecting culture water by a bleaching powder, wherein the disinfected culture water can be used after three days; (2), properly irrigating the culture water to the culture pond before putting young shrimps, splashing bacillus preparations and effective microorganisms (EMs) over the whole culture pond, and putting the young shrimps into the culture pond after three days, wherein the bacillus preparations are activated by brown sugar, and the EMs are subjected to fermentation culture by utilizing aerated water of the disinfection tank; (3), no needing of discharging wastewater within 20-40 days before culturing, only adding water once at every three days, and adding the bacillus preparations and the seawater-cultured EMs at every six days; (4) starting discharging a small amount of wastewater once at the morning and the afternoon of every day after culturing for 20-40 days, changing the water once at every three days, and the like; 2, feed management: (1), ensuring the fact that the feeding frequency is different according to different culture time; (2), adding shrimp and crab nutrients, immune polysaccharides and rice vinegar to the fed feed after culturing for 30 days; (3), controlling the feeding amount after the total feeding amount per mu at every day is stable; 3, emergency processing and the like. According to the disease comprehensive prevention and control method for the greenhouse-cultured penaeus vannamei, the explosion of shrimp diseases can be prevented, and the survival rate and the growth rate of penaeus in the culture procedure can also be remarkably increased.

The invention relates to a method for cultivating highly-healthy young penaeus vannamei. The method is characterized by comprising the following steps of: establishing a young penaeus vannamei cultivating shed; preparing a cultivating feed: smashing a penaeus vannamei 0# material, and adding compound vitamin & amino acid soluble powder; stocking young penaeus vannamei: before stocking young penaeus vannamei, scattering Sanbao high-stability VC (Vitamin C) into a pond; stocking young penaeus vannamei at the density of 1,000-1,500 young penaeus vannamei per square meter; after stocking young penaeus vannamei, starting a bottom layer automatic aerator, and feeding the cultivating feed; gradually adding water in a shed cultivating period according to the conventional feeding cultivating period, adding Sanbao high-stability VC and rhodop seudanonas palustris every three days, and scattering EM (Effective Microorganisms); disinfecting: disinfecting with Sanbao organic iodine once every week in a young penaeus vannamei cultivating period; and cultivating young penaeus vannamei for 25-30 days till the young penaeus vannamei grow to 8-9 centimeters, wherein the survival rate of young penaeus vannamei is 70-85 percent. Due to the adoption of the method, the survival rate of young penaeus vannamei can be increased, and the cultivated young penaeus vannamei are larger than ordinary young penaeus vannamei.

The invention relates to a cultivation method of organic selenium-enriched dendrobium officinale. The method is characterized by comprising the following steps: step I, arranging a bracket used for cultivating dendrobium officinale in a greenhouse in an erecting manner; step II, paving a selenium-enriched organic matter seedbed on the bracket; step III, sterilizing the seedbed by using electro-generative ionized water; step IV, planting dendrobium officinale in the sterilized seedbed, and spreading organic matter subjected to selenium enrichment to serve as a surface fertilizer; step V, controlling the temperature, moisture and illumination in the greenhouse to enable the dendrobium officinale to survive and start to grow; step VI, applying a special fertilizer formed by mixing special inocula for selenium-enriched leaf vegetables with selenium-enriched organic matter once every three days successively for three times after planting survival; step VII, spraying a mixed fertilizer formed by 0.0008-0.001% of electro-generative ionized water and 0.008-0.01% of special inocula for selenium-enriched leaf vegetables once before harvesting of dendrobium officinale; VIII, immediately spraying the mixed fertilizer once after harvesting of dendrobium officinale. According to the cultivation method of organic selenium-enriched dendrobium officinale, the obtained dendrobium officinale is high in selenium content, and the nutritive value is obviously improved.

The invention discloses a method for making salted pepper. The method includes the steps that American red pepper is used as raw materials, after the American red pepper is selected and washed, and surface moisture is removed, and the American red pepper is placed in a salted tank in the mode of 'one layer of red pepper and one layer of salt'; the stacking thickness of each layer of pepper is 5cm, and the salt using amount is 25% of the pepper weight; after pressure weight is added to the salted tank, water is added to the salted tank to completely steep the pepper, salt is added, and then the situation that the salt content in the salted tank is at least 22% is kept; the salted time ranges from 45 days to 60 days, within the period, deposited high-concentration salt water at the bottom of the salted tank is pumped by a sucking pump and then sprinkled to the pepper on the uppermost layer through a sprinkler pipe above the salted tank, the pepper is sprinkled every three days, the pumping and cyclically sprinkling process each time lasts 12 hours every time until the 45-60-day period is finished; the pressure weight is removed, and then the pepper is subpackaged and stored. According to the method for making the salted pepper, pepper salting can be popularized, industrialized production can be formed, development of local industries can be promoted, economic incomes of pepper growers can be also increased, and local economic development is promoted.

The invention discloses a method for treating rotten vegetable leaves and fermenting a biofertilizer by utilizing a biotechnology. The method comprises steps as follows: the rotten vegetable leaves are poured into a treatment tank, a compound decomposer is scattered every time the rotten vegetable leaves are stacked to be 15-25 cm thick, the rotten vegetable leaves and the compound decomposer are repeatedly added to reach the two-thirds position of the height of the treatment tank, water is added until the treatment tank is full, and the treatment tank is covered with waterproof cloth; the rotten vegetable leaves are decomposed for 7-10 days through the compound decomposer, livestock manure, decomposed coal, ammonium sulfate and a compound microbial agent are added to the treatment tank and stirred uniformly for retting, the mixture is stirred once every three days, and retting ends after the mixture is biologically fermented and composted for 15-20 days; after fermentation ends, the mixture is uniformly stirred, and a solid and liquid mixture in the treatment tank is pumped out by a feces suction truck and taken as a biofertilizer to be applied. The raw materials are easy to obtain, the rotten vegetable leaves can be treated on the spot or in the vicinity in a large-scale and harmless manner, a process is simple, energy consumption is not required, no three wastes are generated, the ecological environment is protected, the waste is turned into valuable, and the prepared biofertilizer can be directly returned to the farmland.

The present invention discloses a method for raising pigs with marbled pork, which includes: selecting boar piglets with four weeks pig age, in the fourth week to the eighth week, feeding the boar piglets with the first fodder at six am, twelve pm, four pm and seven pm every day, and massaging the boar piglets with olive oil every other day; in the ninth week to the nineteenth week, feeding the boar piglets with the second fodder at eight am, one pm and seven pm every day, massaging the boar piglets with olive oil every four days, and carrying out a sand bath massage on the boar piglets every other day; in the twentieth week to the twenty fourth week, feeding the boar piglets with the third fodder at eight am, one pm and seven pm every day, massaging the boar piglets with olive oil every three days, and massaging the boar piglets with beer every other day. A scientific method for raising pigs with marbled pork designed by the present invention can effectively improve the quality of marbled pork, and meanwhile effectively improve the quality and taste of pork.

The invention provides a method for separating and culturing human umbilical cord mesenchymal stem cells, which comprises the following steps: taking umbilical cord of fetus born after the normal period of gestation by caesarean section, flushing by PBS under aseptic condition, removing the bloodstain; using an aseptic scissor in an animal cell medium to cut the washed umbilical cord to tissue blocks, removing blood vessel and crushing, then fully mixing with the animal cell medium and oscillating, centrifuging, mixing the centrifuged deposition and a MesenCult-XF complete medium and oscillating, and uniformly planting in a cell culture dish, placing in an incubator with the temperature of 37 DEG C and 5% of CO2 for culturing for 5 days, sucking the tissue blocks out and discharging, refilling the MesenCult-XF complete medium, and replacing the medium every three days for continuously culturing to obtain the umbilical cord mesenchymal stem cells. According to the invention, the animal serum is used, the operation is simple, the repeatability is good, the security is high, and the obtained cell enables stabilization through multitime passage.

The invention discloses a method and a method for reduction treatment of residual sludge. After entering an aerobic biochemical reaction basin for aeration and mixing, a mixture of sludge and sewage is led to a settlement basin for settling, and supernate is extracted; the sludge in the settlement basin is periodically drawn into an anaerobic reaction basin for reacting and static settling; equivalent statically-settled supernate in the anaerobic reaction basin is drawn out to flow back to the aerobic biochemical reaction basin; and equivalent sludge in the anaerobic reaction basin is drawn out every three days to flow back to the aerobic biochemical reaction basin, so as to accomplish circulated exchange and flow of the sludge among settlement equipment, anaerobic equipment, aerobic equipment and the settlement equipment. The method of statically settling the supernate in the backflow anaerobic reaction basin is adopted, the concentration of the sludge in the anaerobic reaction basin is increased, the sludge reduction effect is good and stable, and the denitrogenation ability of a process system is improved; in addition, as the concentration of the sludge in the anaerobic reaction basin is increased, and the volume of the anaerobic reaction basin is reduced, constructing and transforming are easy, no reaction devices and equipments are required to be added, the construction cost is low, and the method and the device have a long-term economic benefit.

The invention discloses a method for preparing bio-organic bacterial fertilizer by composting and aerobic fermentation of edible fungus residues. The method is characterized in that a slant surface ofstains is washed with sterile water to obtain a bacterial suspension, the bacterial suspension is added into a seed bottle under aseptic conditions and cultured to obtain a seed liquid, the seed liquid is inoculated into a fermentation bottle for enlarge culture to obtain a fermented bacterial suspension, and the fermented bacterial suspension is mixed with dried and sterilized bran for culture and then placed in a sterile room for naturally drying to obtain a solid composite bacterial agent; the edible fungus residues are mixed and stirred uniformly with cow dung and straw, turning is conducted once every three days, the water content is adjusted, the solid composite bacterial agent is added twice, and aerobic composting is conducted; water is added into the edible fungus residues, the humidity is adjusted, the edible fungus residues are piled into a conical shape, and fermentation is conducted for one week; bacillus subtilis bacterial liquid, bacillus licheniformis bacterial liquid,bacillus mucilaginosus bacterial liquid and bacillus laterosporus bacterial liquid are mixed with water and uniformly stirred with fermented fungus residue heaps, fermentation is continued, turning treatment is conducted during fermentation, and the mixture above is subjected to composting and uniform mixing with edible fungi to obtain the bio-organic bacterial fertilizer.

The invention provides a method for breeding muscovy ducks. The method includes steps of disinfecting breeding eggs; arranging the breeding eggs; hatching the breeding eggs and the like. The method particularly includes that the eggs are cooled once every three days when hatched on the 11th-31st days, egg vehicles are repositioned after the eggs are completely cooled, and the eggs continue to be hatched; egg cooling operation is carried out in wet-cooling or dry-cooling modes, the breeding eggs are primarily illuminated on the tenth days, and the breeding eggs without hatching value are removed; the breeding eggs are secondarily illuminated on the 17th days; tray turning operation is carried out after the 31st-32nd days; ducklings are primarily picked once on the 34th and 35th days and are primarily captured when the duckling rate reaches 10%; ducklings are secondarily picked when the duckling rate reaches 70% at least. The method for breeding the muscovy ducks has the advantages of high duckling rate and adaptability to requirements of large-scale industrial farming.

The invention provides an iris seed ball treatment method which is characterized by comprising the following steps of: before an iris seed ball is planted, refrigerating for 20 days at 8-9 DEG C; illuminating for 7 days after refrigerating, wherein the illumination intensity is 200-300J / cm<2>, and the temperature is 18-20 DEG C; shading with a 50% shading net, cooling to 13-15 DEG C, and treating for 3 days; and then, carrying out routine management under the conditions that the temperature is 18-21 DEG C and the humidity is 75-80%, i.e. watering 600 times liquid (weight / volume, i.e. 600ml of water is added to 1g of Huaduoduo No.1 (20-20-20)) of Huaduoduo No.1 (20-20-20) every three days.

The invention relates to a method for treating breeding water by composite bacteria. The invention adopts the technical proposal that: 1.5 to 3.0 percent of the composite bacteria accounting for the total weight of the breeding water is thrown into the breeding water every three days, wherein the composite bacteria is mixed bacteria of photosynthetic bacteria and streptococcus faecalis according to a weight ratio of 1:0.5-1.5. The method has low cost and stable action effect, and can effectively improve the water quality of the breeding water, enhance the resistance capability of aquatic organism, and improve the survival rate of the aquatic organism.

The invention relates to a liquid state quick-acting brewing technology for multi-flavored sauce. The liquid state quick-acting brewing technology comprises the process steps of a crushing and refining step of raw materials, a liquid state sauce yeast fermenting step, a heating and sterilizing step, a seasoning step, a sealing step and the like. The liquid state quick-acting brewing technology comprises the following specific steps of: respectively weighing main materials, accessories and spice according to fragrant rice to maize to soybean to black bean to potato to anise to cinnamon being 1:2:2:2:2:0.05:0.05, cleaning and then feeding the mixture into a crushing machine and crushing into powder raw materials; feeding the powder raw materials into a fermentation vat or jar; adding yeast and water according to the ratio of the raw materials to the sauce and the purified water being 100:(0.5-0.6):(300-350) and uniformly stirring; tightly sealing a vat or jar mouth and carrying out anaerobic fermentation for 9 days; stirring once every three days during the period; when a soy sauce mash liquid becomes dark reddish brown and is fermented to be mature, pumping sauce mash into high-efficiency equipment for heating and sterilizing; and filtering by using a special device and carrying out natural green adding according to the ratio of 100:(0.6-0.8):(0.5-0.9) to obtain the multi-flavored sauce with the advantages of excellent color, aroma and taste and ultra-long refreshing time and guarantee period.

The invention provides a high-yield laying hen breeding method. The method comprises the following steps of 1, implementing a brooding period, wherein healthy and disease-free chicks are selected and placed in a brooding house, the chicks are placed at the temperature of 34-36 DEG C to be bred, wherein the temperature is lowered by 2 degrees every week, till the temperature reaches 24 DEG C, and heat supplying equipment is utilized to supply heat; the humidity is 70%, when the chicks are born not soon, the chicks are illuminated for 24 h every day, and the illumination duration is reduced by 1 h every three days; the chicks are subjected to all-day-feeding from the day when the chicks are born till the chicks are 6 weeks old, and the chicks intake food freely; when the chicks are 7 weeks old to 8 weeks old, the chicks are fed with feed every other day; 2, implementing a growing period, wherein the laying hens of 9 weeks old to 18 weeks old are placed on a growing house; 3, implementing an egg laying period, the laying hens of 19 weeks old are fed every day, the situation that every hen is fed with 105 g of feed is every day is maintained, and when the laying hens are 46-72 weeks old, the laying hens are fed every day; 4, adding of a traditional Chinese medicine composition; 5, conducting epidemic prevention; 6, improving the environment of the chick houses. The high-yield laying hen breeding method which effectively increases the egg laying rate is provided.