63 results about "Culture Procedure" patented technology

The invention relates to a method, a system, apparatus, and software to be used for the method for the determination of the environmental impact of the production of cultivation plants and for increasing or maximizing a positive environmental impact. In the method, production parameters for cultivation are selected and cultivation procedures are performed, crop yieldhus produced is harvested, and a representative sample of the crop yield is delivered to reception analysis with the appendant information for the analysis of energy and carbon dioxide factors. An environmental impact index reflecting the environmental impact of the production of said cultivation plant is determined on the basis of this data and the appendant information. The index thus provided is utilized for the production of cultivation plants in an environmentally friendlier manner.

The invention discloses a method for producing a Fujian jewel orchid by plant tissue culture, which comprises the following three procedures: selection and sterile treatment of plants, induced culture of cluster buds and large-scale culture. The method is technically characterized in that two different culture media are used in the induced culture procedure and the large-scale culture procedure, wherein the culture medium for the induced culture comprises an MS culture medium, sucrose of 10-50g / L, agar of 6-8g / L, activated carbon of 2.0-3.0g / L, naphthylacetic acid of 0.1-3.0mg / L and 6-benzylaminopurine of 0.1-5.0mg / L, and the pH value is 5.0-7.0; and the culture medium for the large-scale culture comprises a modified MS culture medium, calcium nitrate of 600mg / L, mashed banana of 30-50g / L, sucrose of 20-40g / L, agar of 6-8g / L, activated carbon of 2.0-3.0g / L, naphthylacetic acid of 1.0-2.0mg / L and 6-benzylaminopurine of 2.0-3.0mg / L, and the pH value is 5.0-7.0. Plant tissue culture can keep the good characters of the plants. The method has the advantages of easy operation, low production cost, high yield, good quality and no environment pollution, is suitable for industrial seedling raising and can realize mass production.

A method for cultivating Se-enriched garlic cloves and garlic bolts features that beside conventional cultivation steps including sowing, thinning out seedlings, applying fertilizer, etc or use of water culture procedure, the sodium selenite (or selenate) and nutritive liquid are mixed to obtain the liquid selenium fertilizer, which has different concentrations of inorganic selenium for different growth phases. Said garlic cloves and garlic bolts feature their health-care function.

The invention discloses a water-saving and fertilizer-holding seedling culture substrate, which is prepared by compounding vermiculite and water retention agents in certain proportion, wherein the vermiculite serves as a base material; and the water retention agents have different particle sizes and fully absorb high-concentration fertilizers. The invention also relates to a method for preparing the substrate and the method for performing vegetable plug seedling culture by using the substrate. The seedling culture substrate and the method for performing the vegetable plug seedling culture have the advantages that: seedlings are watered only with clear water in a seedling culture period, so seedling culture procedures are greatly simplified, labor efficiency can be improved by 0.5-1 time, the seedlings are grown fast and have developed root systems, the seedling culture period is shortened by 5 to 7 days, fresh water and turf resources can be saved and the cost is reduced by 20 to 30 percent.

The invention provides a high active induction culture method for lily bulbil which is characterized in that: through an induction culture of embryoid and tissue enrichment culture of embryoid tissue, a relative high rapid propagation velocity is obtained; then through an differentiation culture of bulb-lets and an intumescence culture of bulb-lets, transplanting bulb-lets of a circumferential diameter bigger than 3cm are obtained. The growth rate of the bulb-lets of the invention can reach more than one million grains per years which is ten times that of conventional methods. With a large number of small bulb-lets obtained, the ratio of bulb-lets with large specification and large grain size is increased. The invention optimizes the culture procedures of preferential lily seedball, shortens the propagation time of preferential lily seedball; furthermore, the field planting survival rate of bulb-lets is high and the production cost is low; the invention is suitable for the scale rapid propagation production of cut lily elite.

The invention relates to a rapid and high-flux acute toxicity test method for luminous bacteria, belonging to technical fields of analysis and detection. A luminous bacteria reviving solution is developed and can be used for rapidly reviving lyophilized luminous bacteria powder and cryopreserved strains within 30min, and the revived bacterium liquid can be directly used for a toxicity test as a working bacterium liquid without pre-culture after being stirred for a certain time. Meanwhile, a microporous plate is selected to serve as an exposing container and a detection tank for the toxicity test, a sample to be tested and the working bacterium liquid are added into pores and exposed for 15min, and then can be subjected to a luminous intensity test by adopting a chemiluminescence ELISA (Enzyme-Linked Immunosorbent Assay) reader. By adoption of the method, not only is a tedious and time-consuming working bacterium liquid pre-culture procedure omitted, but also the detection time is saved, the cost is reduced, the accuracy of a test result is increased, the high flux of the toxicity test is realized, and a wide popularization and application prospect is achieved.

The invention provides a disease comprehensive prevention and control method for greenhouse-cultured penaeus vannamei. The disease comprehensive prevention and control method for the greenhouse-cultured penaeus vannamei is characterized by comprising the following steps: 1, water body regulation and control: (1), matching one culture pond with one disinfection tank, and disinfecting culture water by a bleaching powder, wherein the disinfected culture water can be used after three days; (2), properly irrigating the culture water to the culture pond before putting young shrimps, splashing bacillus preparations and effective microorganisms (EMs) over the whole culture pond, and putting the young shrimps into the culture pond after three days, wherein the bacillus preparations are activated by brown sugar, and the EMs are subjected to fermentation culture by utilizing aerated water of the disinfection tank; (3), no needing of discharging wastewater within 20-40 days before culturing, only adding water once at every three days, and adding the bacillus preparations and the seawater-cultured EMs at every six days; (4) starting discharging a small amount of wastewater once at the morning and the afternoon of every day after culturing for 20-40 days, changing the water once at every three days, and the like; 2, feed management: (1), ensuring the fact that the feeding frequency is different according to different culture time; (2), adding shrimp and crab nutrients, immune polysaccharides and rice vinegar to the fed feed after culturing for 30 days; (3), controlling the feeding amount after the total feeding amount per mu at every day is stable; 3, emergency processing and the like. According to the disease comprehensive prevention and control method for the greenhouse-cultured penaeus vannamei, the explosion of shrimp diseases can be prevented, and the survival rate and the growth rate of penaeus in the culture procedure can also be remarkably increased.

Development of an efficient and cost-effective doubled haploid production system and genetic transformation system are the prerequisite to initiate haploid breeding and genetic modification in flax respectively. Pre-culturing anthers on a high osmotic, high auxin and high mineral salt concentration for a period of time before transfer to a low osmotic, low auxin and low salt concentration significantly increased the overall efficiency of regeneration or anther efficiency than directly culturing anthers on a low osmotic, low auxin and low salt concentration medium. This culture procedure also dramatically reduced the frequency of shoot regeneration from somatic cells in anther culture. Using this procedure, a highly efficient anther culture-derived callus based transformation system was developed. The transformation efficiency of anther culture-derived callus based transformation system was four times higher than the best reported transformation efficiency using hypocotyls as the ex-plants in Agrobacterium tumefaciens based transformation system or particle bombardment based transformation system. The frequency of escape in anther culture-derived callus based transformation system was one third of that in hypocotyl-based transformation system using A. tumefaciens or one half using particle bombardment. This very high efficient transformation system will prove to be very valuable in basic research for gene discovery and practical applications in genetic engineering for improved traits.

The invention discloses a solid culture medium for inoculating a great amount of liquid samples and a culture method. The solid culture medium is provided with a culture layer, wherein the culture layer at least comprises a liquid sample adsorption layer; the liquid sample adsorption layer is a water absorption membrane layer and / or a water absorption powder layer. The solid culture medium can absorb liquid in the liquid sample, a great amount of liquid samples can be directly inoculated and cultured, and then counting, observation, separation or identification is further carried out on the solid culture medium according to a purpose. The solid culture method for a great amount of liquid sample microorganisms is characterized in that a great amount of liquid samples are poured into the solid culture medium to be subjected to solid culture, the solid culture does not need to be followed by the liquid culture, the culture and separation can be integrated according to the requirement, the microorganisms can be quantified while being cultured, a bacterial colony can be separated, the positive rate of the cultured microorganisms can be increased, the culture procedure can be simplified, the culture time can be saved, and the timeliness can be improved.

A population of myeloid-derived suppressor cells and the culture procedure to obtain these in vitro starting with bone marrow cells of mice, other animals and human beings, in the presence of specific cytokine combinations used to determine concentrations, is provided.

The invention discloses a biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, a method for preparing the biological nanometer material and application thereof. The method includes steps of carrying out contact culture on the psychrobacter aquimaris X3-1403 by the aid of culture media which at least contain calcium, phosphorus, oxygen and hydrogen elements; centrifugally removing supernatant to obtain the biological nanometer material after contact culture is carried out. The psychrobacter aquimaris X3-1403 belongs to psychrobacter and is already preserved at the China Center for Type Culture Collection in Luojia Hill of Wuchang of Wuhan City on 29 March, 2016, and a preservation number of the psychrobacter aquimaris is CCTCC M 201655. The biological nanometer material, the method and the application have the advantages that psychrobacter aquimaris culture procedures are nanometer hydroxyapatite and biological nanometer material synthesis procedures, conditions are mild during culture, the biological nanometer material is easy to operate, free of pollution, low in cost and high in efficiency and is clean, and the biological nanometer material and the method can be popularized and applied on a large scale.

The invention relates to equipment for carrying out bacterial culture and automatic implantation on a blood sample and particularly relates to an automatic implantation type blood culture instrument. According to the automatic implantation type blood culture instrument, three culture tanks are arranged, a first culture tank is used for arranging blooding culture bottles and corresponding detection devices, and a streak inoculation site, a delivery mechanical arm and a streak mechanical arm are arranged among the first culture tank, a second culture tank and a third culture tank, so that the timed implantation capacity of a positive sample is greatly improved. Compared with products in the prior art, the automatic implantation type blood culture instrument has the characteristics that by virtue of the delivery mechanical arm and the streak mechanical arm, the streak inoculation and isolated culture procedures of a positive blood sample can be automatically finished by the instrument; the implantation of the positive sample is realized under a culture environment, and the timed implantation capacity of the sample is improved; by implanting the sample to a solid culture medium under the culture environment, the positive rate of the implantation is increased; the labor force of professionals in a microbiological lab is liberated, and the blood culture quality is improved.

The invention discloses a rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum. Hybrid cymbidium aseptic seedling base tissue of the cymbidium sinense and the cymbidium hookerianum is used as a raw material, induction culture is performed in an induction culture medium containing various plant growth regulators and additives, protocorms are obtained and subjected to proliferation and differentiation culture, and then seedling strengthening, rooting and transplantation are performed. Steps of proliferation and differentiation of the protocorms are combined, the culture procedure is simple, the material cost for preparation of the culture medium is greatly reduced, the labor cost for preparation of the culture medium and inoculation is also reduced, the inductivity of the protocorms is high, the quantity of the protocorms is large, the proliferation coefficient is large, and the method has multiple advantages of low production cost, high seedling quality, short culture period, avoidance of variation and the like.

The invention belongs to the technical field of fermentation engineering, and especially relates to a fermentation medium and a fermentation method for producing aflatoxin B1. The fermentation medium of aflatoxin B1 contains a yeast extract which accounts for 1-5% of the total weight of the medium; the fermentation medium comprises a recovery medium, a solid fermentation medium or a growth promotion nutrient solution. The fermentation method of aflatoxin B1 comprises two steps of strain recovery and solid fermentation culture; aspergillus flavus is employed for fermentation; the fermentation medium provided by the invention is employed for strain recovery and solid fermentation culture. According to the medium and culture procedure provided by the invention, the concentration in a solid air-dried substance of the fermentation medium of aflatoxin B1 is up to 7349 ppb; when the fermentation medium and the fermentation method of the invention are compared with the prior art with respect to fermentation titer, significant advancement is provided.

The invention discloses a landscaping seedling cultivation and culture integrated device. The integrated device comprises a soil loosening wheel, a cultivation frame, a first motor, a rotating disc, a guide pipe, a second motor, a second gear, a rack, a first limiting block, a connecting rod, an electric push rod, a second limiting block, an integration wheel, a water tank, a water outlet pipe, a ball valve and a spray head. By using the cultivation and culture integrated equipment, a cultivation procedure and a culture procedure are integrated into one procedure, so that manpower and material resources are greatly saved; through cooperative use of the soil loosening wheel, the cultivation frame, the first motor, the rotating disc, the guide pipe, the second motor, the second gear, the rack, the first limiting block, the connecting rod, the electric push rod, the second limiting block and the integration wheel, an automatic cultivation structure is formed, manual cooperation is further reduced, and use is convenient; and through cooperative use of the water tank, the water outlet pipe, the ball valve and the spray head, a spraying irrigation structure is formed, watering culture is directly conducted after cultivation, and use is convenient.

The invention relates to a method for promoting rooting of tissue culture seedlings of blueberry, which mainly solves the technical problems of multiple culture procedures, low survival rate, long period and the like in the rooting of tissue culture seedlings of the blueberry. The method comprises the following steps: (1) bottle seedling selection; (2) seedlings hardening; (3) tissue culture seedlings transplantation; and (4) post-transplantation management. An effective rooting technique of blueberry tissue culture seedling is established and the tissue culture and rapid propagation system isoptimized. The technique does not need to go through the rooting procedure in a tissue culture bottle, rooting and domestication are carried out at the same time, and a large amount of roots are produced in the seedling base after 25 to 30 days. A robust seedling base is obtained after 2 months. Compared with traditional bottle rooting, the seedling cycle is shortened by more than one month, andthe transplanting survival rate is more than 95%. Besides, the external rooting technology of the bottle can reduce the production cost by about 40% and save tissue culture space by more than 50%, which is of great significance for intensive production of the blueberry tissue culture.

The invention relates to a culture method and a culture device for aerobic granular sludge. The culture method for the aerobic granular sludge particularly comprises the following steps of inoculatingactive sludge in a reactor, adding PAC (Polyaluminum Chloride) and sepiolite, and mixing the active sludge; pumping artificial synthetic wastewater into the reactor by utilizing a water intake device, and culturing the aerobic granular sludge through an SBR (Sequencing Batch Reactor) treatment process. The culture device is used for solving problems that the start time is long, the running is unstable, the impact load resisting ability is weak and sludge is easily disintegrated, and the like, in a culture procedure of the aerobic granular sludge, can be used for enhancing biological phosphorus removal, and has advantages of synchronous denitrogenation and phosphorus removal function, stable running, wide raw material source, simple operation and the like.

A method for immunizing the meat geese in their centralized culture procedure in order to increase their survival rate includes such steps as injecting the double yolk liquid of small goose pest and viral enteritis in one-day age, injecting the double vaccine of colibasillus and fowl's cholera vibro in 3- day age, injecting the double vaccine of fowl's influenza virus and paramyxovirus in 5-day age, injecting the double vaccine of fowl's influenze virus and paramyxovirus in 15-day age, and injecting duck pest vaccine in 20-day age.

The invention belongs to the technical field of cell culture and in particular relates to a canoidea dermal fibroblast cell primary culture method. The canoidea dermal fibroblast cell primary culturemethod comprises the following steps: (1) scissoring a thoracoabdominal skin tissue of an adult dog; (2) cleaning the skin tissue, removing subcutaneous fat and connective tissues and cutting the skintissue into pieces and transferring the skin tissue to a centrifugal tube; (3) adding 1 mL II collagenase solution into the centrifugal tube and scissoring the solution into paste, adding 5 mL II collagenase, uniformly mixing, putting the solution in a culture box till tissue slurry is digested fully; (4) taking out the centrifugal tube for centrifugalization, abandoning a supernate, adding a culture medium to re-suspend, filtering the re-suspended solution with a cell screen, carrying out centrifugalization again on the filtrate, abandoning the supernate, adding a cell culture solution to re-suspend, then transferring the solution to a hexagonal porous plate, and adding the culture solution; and (5) putting the solution in the culture box to stand to be cultured for 48 hours, and replacing a fresh culture solution every day till the canoidea dermal fibroblast primary cells are obtained. The method is low in operating difficulty, simple in culture flow and relatively high in culture efficiency.

The invention discloses a method for obtaining an African daisy regeneration plant through inducing a somatic embryo. The method comprises the steps that an African daisy receptacle serving as an explant material is cultured in a somatic embryo inducing culture medium containing 6-benayl aminopurine, kinetin, thidiazuron, naphthylacetic acid, picloram and casein hydrolysate; the somatic embryo is induced; after the somatic embryo germinates, the somatic embryo is subjected to successive transfer culture in a differential medium; and the regeneration plant is obtained. According to the method, the African daisy receptacle serves as an explant, and the regeneration plant is obtained through a somatic embryo approach without a callus stage, so that a situation that explant is mutated due to dedifferentiation inducing callus regeneration can be avoided. By utilizing the method to perform African daisy tissue culture for seedlings, the culture procedure is simplified, the culture cycle is shortened, the quality of the seedlings is improved, and base materials are provided for a large number of high-quality seedlings by in-vitro propagation of African daisy and transgenic research.

The invention provides a method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine. The method has the advantages that bacteria solution is prepared from bacillus rhusiopathiae suis in fermentation modes in preparation procedures, a pH (potential of hydrogen) value of fermentation broth is strictly controlled in bacteria solution fermentation procedures, accordingly, the immunogenicity of bacillus rhusiopathiae suis antigens can be effectively guaranteed, and the protection rate can be higher than 90% without excessively high quantities of thalli of the bacillusrhusiopathiae suis; viruses can be reproduced from porcine parvovirus in IBRS-2 cell suspension culture modes by the aid of bioreactors in swine erysipelas and porcine parvovirus bivalent inactivatedvaccine preparation procedures, and accordingly the titer of virus liquid can be obviously improved; dissolved oxygen, pH, rotational speeds and the like are set in virus reproduction procedures, accordingly, the homogeneity of inter-assay virus liquid can be guaranteed, the shortcomings of complicated operation, high labor intensity, vulnerability to pollution and the like in spinner-bottle culture procedures can be overcome, and the quality stability of porcine parvovirus inactivated vaccine can be effectively enhanced.

The invention discloses a method for culturing atrial muscle cells of rats, and belongs to the technical field of cell culture. The method includes steps of material acquisition, digestion, separation, centrifugation, inoculated culture and the like. The method for culturing the atrial muscle cells of the rats has the advantages that the method is low in cost and high in efficiency and is stable,separated cells are good in viability and high in purity, and the atrial muscle cells are high in quantity and yield; effects of protecting the cells can be improved by the aid of the method in culture procedures, accordingly, the viability of the cells can be improved, and the cells are high in survival rate; medicines such as penicillin and Brdu are omitted in the culture procedures, and accordingly potential impact of the penicillin and the Brdu on follow-up research can be prevented.

The present invention relates to a manufacturing method of immune killer cells characterized in that an immune killer cell is induced in a culture medium containing concanavalin A (ConA), and the immune killer cell is maintained or expanded in a culture procedure. Antibody proteins are not used as a stimulant in the culture process to avoid the risk of being infected by zoonotic diseases and effectively increase the number and specific release of the immune killer cells.

The invention discloses a culture medium for culturing antrodia camphorata by vessel culturing and a preparation method thereof. The culture medium is prepared from the following raw materials in parts by weight: 200 to 300 parts of potato leaching powder, 20 to 40 parts of glucose, 0 to 20 parts of soluble soybean meal, 1 to 20 parts of malt extractant, 0.5 to 1.5 parts of magnesium sulfate, 10 to 60 parts of a mixture of a cinnamomum plant, 0.5 to 1.5 parts of potassium dihydrogen phosphate, 0.01 to 0.05 part of vitamin B1, 0 to 0.1 part of chloramphenicol, 15 to 25 parts of agar and 1,000 parts of ultrapure water. According to the culture medium disclosed by the invention, a culturing vessel is used for culturing the antrodia camphorata; the culturing condition has strong controllability; the culturing procedure is simplified; the sundry bacteria contamination can be reduced; the heavy metal pollution is avoided too; the growth increment of antrodia camphorata mycelium or sporocarp is increased as well; and the production cost is lowered.

The invention discloses an intelligent automatic pollution discharge system of a prawn intensive culture pond. The intelligent pollution discharge system comprises a power system, a suction system, a positioning system and an oxygen aeration system. The power system comprises a water pump, a driving water pipe and a time switch, the driving water pipe is connected with the water pump, and the water pump can be driven under the control of the time switch; the suction system comprises a suction pipe connected with a central pollution discharge pipe of a pond body; the positioning system comprises a screen frame, a lifting sucker and a 360-degree turntable, the lifting sucker is arranged on the screen frame, the 360-degree turntable is connected with the screen frame, and the oxygen aeration system comprises an oxygen aeration pipe. The intelligent automatic pollution discharge system has the advantages that technical problems that an existing drain pipe is heavy, contact positions of the existing drain pipe and a drain outlet often are adhered with each other and are difficult to pull up manually after the existing drain pipe is used for a long time, labor can be wasted, pollution discharge time is difficult to accurately control, workload is high, time can be wasted and the like can be solved; an automatic pollution discharge effect can be realized by the pollution discharge system under the timed control of a servo motor, and accordingly workload can be greatly reduced; a suction range of the suction pipe can be enlarged by the aid of lifting and rotating modes, so that the problem of difficulty in discharging pollution at the bottom of an existing culture pond in culture procedures can be solved.

The invention relates to a method for promoting rooting of ilex verticillata tissue cultured seedlings and mainly solves technical problems of excessive culture procedures, low survival rate, long period and the like in rooting of existing ilex verticillata tissue cultured seedlings. The method includes steps: (1) bottle seedling selection; (2) hardening; (3) ex-vitro cuttage of the tissue cultured seedlings; (4) after-cuttage management. An effective ex-vitro rooting technique for the ilex verticillata tissue cultured seedlings is created, and a tissue-culture rapid propagation system is optimized. The technique avoids an in-vitro rooting procedure, rooting and domestication are carried out at the same time, base parts of the seedlings grow a great quantity of root systems in 25-30d, andstrong rooted seedlings can be obtained in 2 months. Compared with traditional in-vitro rooting, the method has advantages that a seedling cultivation period is shortened by 1 month or more, the rooting rate is greater than 93%, the transplanting survival rate is greater than 95%, production cost can be reduced by about 40%, the tissue culture space is saved by 50% or more, and the method is significant to tissue culture intensive production of ilex verticillata.

The invention belongs to the technical field of tissue culture rapid propagation and discloses an induction medium for nauclea officinalis aseptic seedlings and a nauclea officinalis detoxification rapid propagation method. The induction medium is prepared from a 3 / 4 MS medium which is prepared from 0.1%-10.0% of organic additives and 0.05-0.2 mg / L DA-6. The nauclea officinalis detoxification rapid propagation method is established by optimizing a detoxification method and optimizing aseptic seedling culture, enrichment culture and rooting culture procedures. Compared with a traditional propagation method, the advantages of being capable of saving labor, time and land, low in cost, high in propagation rate (the growth coefficient>30 generations / year), high in emergence rate, short in propagation time and high in survival rate (>95%) are achieved, the purposes of rapid and efficient propagation and large-scale popularization planting of nauclea officinalis are achieved, and a foundation is laid for industrial production of nauclea officinalis seedlings.

The invention relates to a populus hopeiensis genetic transformation method. Aseptic seedling leaves are used as explants for genetic transformation; adventitious buds are directly induced without a callus induction stage; according to the method, a complex culture procedure is omitted, the culture period is shortened, an efficient genetic transformation system of populus hopeiensis is establishedby utilizing an agrobacterium-mediated method, a new populus hopeiensis variety is cultured, function research can be performed on related genes, and the method has important significance for furtherestablishing a quick gene function verification system.

The invention discloses a method for increasing the survival rate of high-density culture fish. The method mainly includes diversified aspects that aerators are uniformly arranged in culture ponds andare distributed in a 'high and low' circulation manner according to arrangement locations, the arrangement density of the aerators is 15-30 aerators / mu, and the aerators can be started at regular time; aquatic vegetables are planted on the surfaces of the culture ponds by the aid of bamboo rafts and are close to shores, the high-density culture fish is fed with biological bait in culture procedures, disease-resistant immune enhancers are mixed with the biological bait, the high-density culture fishes are cultured with elopichthys bambusa in the culture ponds in a matched manner at culture middle stages, and the like. The method has the advantages that the culture water quality can be effectively improved, the immunity of grass carp can be enhanced, accordingly, the survival rate of the grass carp can be increased, and the quality of the grass carp can be enhanced.