10 results about "Culture Procedure" patented technology

This invention provides an application and method of lysophosphatidylcholine (LPC) in promoting early development of reconstructed embryos, belonging to the field of embryo engineering technology. The invention first prepares reconstructed embryos, then adds lysophosphatidylcholine to the culture system for culturing. Lysophosphatidylcholine effectively promotes early development of the reconstructed embryos; at an optimal concentration of 5 μM, the blastocyst development rate increases to 24.56%, providing a novel and operable biochemical intervention target to solve the problem of low reconstructed embryo culture efficiency. This invention not only fills the gap in our understanding of the mechanism of action of LPC in this field, but also achieves controllability and reproducibility of the technical solution through precise definition of the optimal concentration. It provides key parameters for establishing standardized culture procedures and provides a more solid foundation for downstream applications such as developmental biology research and disease model construction that rely on high-quality reconstructed embryos, significantly accelerating the process of related scientific discovery and application development.

The invention discloses a leguminous plant pollen germination method which specifically comprises the following steps: S1, weighing of raw materials of a liquid culture medium, S2, preparation of the liquid culture medium, S3, treatment before culture, S4, sampling treatment, S5, flaking treatment, and S6, germination rate calculation: observing the germination condition of pollen, taking the condition that the length of a pollen tube exceeds the diameter of pollen grains as a germination standard, and calculating the germination rate. And calculating the germination rate: the germination rate = (the number of germinated pollen / the total number of observed pollen) * 100%. The invention relates to the technical field of plant culture. According to the leguminous plant pollen germination method, simple and rapid culture treatment can be carried out in the pollen germination culture process, more accurate statistical treatment can be carried out through an average statistical method, the culture procedure is simple and reliable, and the statistical process of the pollen germination rate is accurate and persuasive; the aim of quickly and accurately completing pollen germination culture treatment is well fulfilled, so that the method is very beneficial to the research on the pollen germination culture treatment.

The invention discloses a screening and preparation method and application of phagostimulant functional peptide FRQGDVIALP of megalobrama amblycephala, and particularly relates to a method for preparing the functional small peptide FRQGDVIALP (Patt) by using intestinal epithelial cell in-vitro screening, plasmid construction, bacillus subtilis high-efficiency expression and in-vitro pepsase enzymolysis. Compared with the prior art, the screening method is simple, rapid and efficient, compared with an existing product preparation technology, the expression system is simple in culture procedure, compared with the prior art, the production cost of the functional small peptide FRQGDVIALP is low, and the final yield is 25.96%. Compared with the existing product effect technology, the Patt can significantly improve the feed intake and growth performance of aquatic animals in an intensive breeding environment, and has a good food calling effect.

This invention provides a sterility indicator that does not require complex culture procedures like BI or luciferin-luciferase assays like EI, can be supplied stably at low cost, and can accurately measure sterility intensity in a dry state. [Solution] The device comprises a base material and a sensitive member supported on the base material that is sensitive to the sterilizing agent. The sensitive member is made of a material whose constituent is a fluorescent protein, and genetically modified fluorescent silk is used. This genetically modified fluorescent silk emits fluorescence at a predetermined wavelength when irradiated with excitation light of a predetermined wavelength. The fluorescence intensity of the sensitive member changes in proportion to the exposure intensity of the sterilizing agent to the sensitive member.

The utility model discloses a multichannel tubular photo-oxidation bioreactor device, which relates to the technical field of microalgae culture and online monitoring and comprises a water bath box, a gas regulation and control module, a dissolved oxygen monitoring module, an optical density monitoring module and a PH (potential of hydrogen) monitoring module. A glass culture tube and a temperature control module are mounted in the water bath box, the dissolved oxygen monitoring module extends into the glass culture tube through a probe or a sensor, and the optical density monitoring module is in contact with the glass culture tube through an optical sensor or a probe, so that accurate culture conditions are provided, and the microalgae culture efficiency is improved; according to the device, a controllable algae culture environment is provided, meanwhile, real-time monitoring of dissolved oxygen, OD value, PH and temperature is achieved, collection and analysis of scientific research data are facilitated, a user can conveniently set a culture program and check measurement data through a graphical interface of a control system, and the device is convenient to install and use due to the compact machine body structural design.

This planning system comprises: an acquisition unit for acquiring a sequence representing the order of execution of culture procedures of a project, as well as a start time or end time for the project; a creation unit for creating a proposed schedule that has an established start day and end day for the project as well as an execution period for each of the culture procedures; a calculation unit for calculating a predicted value for a total used quantity, per unit time, of resources for the project and another project; a modification unit for modifying the proposed schedule by moving the execution period of a subject step ahead or behind so as to satisfy a first constraint condition; and an output unit for outputting the proposed schedule for the project.

The embodiment of the invention discloses a heterogeneous software intelligent interaction method and device applied between biological laboratories. A specific embodiment of the method comprises the following steps: generating a culture process file; performing culture program self-inspection on heterogeneous software corresponding to the heterogeneous software identification group included in the culture process file; constructing a software temporary interaction layer; controlling the automatic culture equipment to perform biological sample culture operation on the placed biological sample, and controlling the culture monitoring equipment to shoot a sample monitoring image at a fixed angle and synchronously collect a sample spectral signal; and performing sample pollution detection on each shot sample monitoring image and the corresponding sample spectral signal to generate a sample pollution detection report. According to the embodiment, whether the detection sample is polluted or not can be confirmed, and meanwhile the compatibility problem between heterogeneous software is solved.