6 results about "Culture Procedure" patented technology

This invention provides an application and method of lysophosphatidylcholine (LPC) in promoting early development of reconstructed embryos, belonging to the field of embryo engineering technology. The invention first prepares reconstructed embryos, then adds lysophosphatidylcholine to the culture system for culturing. Lysophosphatidylcholine effectively promotes early development of the reconstructed embryos; at an optimal concentration of 5 μM, the blastocyst development rate increases to 24.56%, providing a novel and operable biochemical intervention target to solve the problem of low reconstructed embryo culture efficiency. This invention not only fills the gap in our understanding of the mechanism of action of LPC in this field, but also achieves controllability and reproducibility of the technical solution through precise definition of the optimal concentration. It provides key parameters for establishing standardized culture procedures and provides a more solid foundation for downstream applications such as developmental biology research and disease model construction that rely on high-quality reconstructed embryos, significantly accelerating the process of related scientific discovery and application development.

This invention provides a sterility indicator that does not require complex culture procedures like BI or luciferin-luciferase assays like EI, can be supplied stably at low cost, and can accurately measure sterility intensity in a dry state. [Solution] The device comprises a base material and a sensitive member supported on the base material that is sensitive to the sterilizing agent. The sensitive member is made of a material whose constituent is a fluorescent protein, and genetically modified fluorescent silk is used. This genetically modified fluorescent silk emits fluorescence at a predetermined wavelength when irradiated with excitation light of a predetermined wavelength. The fluorescence intensity of the sensitive member changes in proportion to the exposure intensity of the sterilizing agent to the sensitive member.

The embodiment of the invention discloses a heterogeneous software intelligent interaction method and device applied between biological laboratories. A specific embodiment of the method comprises the following steps: generating a culture process file; performing culture program self-inspection on heterogeneous software corresponding to the heterogeneous software identification group included in the culture process file; constructing a software temporary interaction layer; controlling the automatic culture equipment to perform biological sample culture operation on the placed biological sample, and controlling the culture monitoring equipment to shoot a sample monitoring image at a fixed angle and synchronously collect a sample spectral signal; and performing sample pollution detection on each shot sample monitoring image and the corresponding sample spectral signal to generate a sample pollution detection report. According to the embodiment, whether the detection sample is polluted or not can be confirmed, and meanwhile the compatibility problem between heterogeneous software is solved.