90 results about "Phospholipin" patented technology

A composite encapsulated vitamin liposome, which is prepared from the following raw materials in parts by weight: 2-65 parts of a vitamin, 15-65 parts of a phospholipid, 0.2-3 parts of an antioxidant, 1-8 parts of a membrane stabilizer, 9-45 parts of a first-layer wall material, and 4-20 parts of a second-layer wall material. A preparation method comprises: preparing an aqueous solution of a water-soluble vitamin to obtain an aqueous phase; fully mixing and dissolving anhydrous ethanol, a phospholipid and a membrane stabilizer, and then adding a lipid-soluble vitamin and allowing same to completely dissolve to obtain an alcohol phase; separately preparing a first-layer wall material solution and a second-layer wall material solution; slowly injecting the alcohol phase into the aqueous phase, and stirring same sufficiently to form a primary liposome dispersion solution; performing vacuum concentration to obtain a concentrated solution, slowly adding same to the first-layer wall material solution, and stirring same sufficiently to form a dispersion solution; and performing high speed shearing and high pressure homogenization and mixing same with the second-layer wall material solution, and performing drying to obtain the final product. The liposome can be used in the production of tablet preparations, hard-shell capsules and solid beverages, and can achieve high stability and controlled release of vitamins.

The application discloses a preparation method of egg yolk phospholipid polypeptide which is helpful for bone growth and improvement of brain health, and comprises the following steps: (1) raw material pretreatment to obtain a reaction substrate solution; (2) primary enzymolysis to prepare a primary enzymolysis solution; (3) secondary enzymolysis to prepare a secondary enzymolysis solution; (4) inactivation, the temperature of the secondary enzymolysis solution prepared in the step (3) is adjusted to 85-95 DEG C, and the enzyme is completely inactivated by keeping for 10-15 min; (5) fermentation; (6) post-treatment, the fermentation liquid after the fermentation is kept at 50-60 DEG C for 10-30 min, and then cooling, centrifugation, collection of supernatant, vacuum freeze drying or spray drying of the supernatant are carried out, so that the egg yolk phospholipid polypeptide powder is obtained. The natural compound rich in specific functional peptide segments and phospholipids is prepared through the two-step enzymolysis coupling fermentation process, can effectively promote the growth and development of the bone, significantly improve the learning, memory and cognitive ability, and can significantly promote the proliferation of osteoblasts.

The present invention discloses a β-carotene-grape seed extract eye-care preparation for retinal protection, relating to functional health foods. The preparation comprises active components and food-acceptable excipients. In parts by weight, the active components include 1–4 parts lutein, 0.5 –2 parts meso-zeaxanthin, 2 – 6 parts β-carotene, 8 – 25 parts blueberry extract, 5 – 18 parts grape seed extract, 0.2–1 part L-ergothioneine, 0.5–3 parts saffron extract, and 1–5 parts phosphatidylserine. The excipients comprise a filler, disintegrant, binder, and lubricant, with a weight ratio of active components to excipients of 1:1 to 1:4. By combining these eight components at specific ratios, the invention achieves synergistic effects, protects retinal health, and reduces blue-light damage. Dual-phase microencapsulation based on solubility, together with standardized preparation steps, improves stability and utilization efficiency.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

Since it separates into at least an emulsion layer and an aqueous layer during standing, it has excellent aesthetic properties. When used, it can be uniformly redispersed by gently shaking, so it also has excellent usability. In addition, it has good storage stability, and further provides a multi-layer cosmetic excellent in the softness of the back skin. 【Means for Solving the Problem】 The following components (A) to (G): (A) Phospholipid (B) Cholesterol and / or phytosterol (C) Phosphoric acid-based surfactant (D) Oil agent (E) Sodium polyaspartate (F) Water-swelling clay mineral (G) Water A multi-layer cosmetic containing the above components.

Described herein are compositions, kits, and methods for potent delivery to a cell of a subject. The cell can be of a particular cell type, such as a basal cell. In some cases, the cell can be a lung cell of a particular cell type. Also described herein are pharmaceutical compositions comprising a therapeutic or prophylactic agent assembled to a lipid composition. The lipid composition can comprise an ionizable cationic lipid, and a selective organ targeting lipid. The lipid composition can further comprise a phospholipid. Further described herein are high-potency intravenous dosage forms of a therapeutic or prophylactic agent formulated with a lipid composition.

The present application relates to a kind of brain health nutrition capsule and its preparation method, belong to the technical field of functional health food.The preparation method includes the following steps: taking walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo biloba extract and oligosaccharides are mixed, soft material is prepared by adding purified water, granulation is extruded by screen, and granule core is obtained by drying;Granule core is coated, and brain nutrition core granule is obtained by drying;Prepare base nutrition layer granule;Brain nutrition core granule and base nutrition layer granule are mixed, and composite granule is obtained, and composite granule is filled into capsule.The present application not only solves the three major obstacles of oxidation, precipitation and vitamin degradation of high polyphenol walnut base formula in gastric acid environment, but also unexpectedly builds from stomach protection to intestinal microecological optimization, finally realizes the cascade effect of brain nutrition efficient absorption, meets the core needs of teenagers brain health and intelligence, and truly realizes the effect of brain health and intelligence.

The present application relates to the field of pet feed, and discloses a maintenance ration formula for improving the smoothness of dog hair. The formula comprises base ration particles and lipid microcapsules attached to the surface of the base ration particles; the lipid microcapsules encapsulate a core material composed of essential fatty acids and ceramides, have a double-layer wall material structure, and the outer layer is acid-resistant plant gum and the inner layer is lipase-sensitive phospholipid; the microcapsules are attached to the surface of the puffed base ration by low-temperature vacuum spraying. The present application makes the microcapsules resistant to gastric acid and releases the core material under the action of duodenal lipase, targets and enriches in sebaceous glands through the lymphatic system, solves the problems of low lipid delivery conversion rate and insufficient substrate of sebaceous glands, improves the proportion of sebaceous gland ceramides, and reduces the hair friction coefficient.

This invention relates to a blueberry lutein ester hawthorn cookie with a protective effect on vision and its preparation method. The raw material composition is as follows: 100-180 parts flour, 20-80 parts palm oil, 20-80 parts maltitol, 1-30 parts roasted wheat bran, 1-15 parts soybean dietary fiber, 1-10 parts polydextrose, 1-20 parts blueberry powder, 1-20 parts hawthorn powder, 1-20 parts leavening agent, 0.1-6 parts flavoring agent, 0.1-3 parts disodium dihydrogen pyrophosphate, 0.1-3 parts phospholipids, 1-5 parts lutein ester, 0.001-1 part water-soluble β-carotene, 0.1-4 parts blueberry flavoring, and 2-50 parts water. This cookie uses lutein ester, blueberry powder, and water-soluble β-carotene in a scientifically formulated ratio. After being absorbed by the human body, these three ingredients work synergistically to relieve eye fatigue and greatly enhance the protective effect on vision. It also has the advantages of good appearance and color, low calories, crispy and delicious taste, while being low in sugar, relieving eye fatigue, protecting the eyes, rich in dietary fiber, strengthening the spleen and aiding digestion, and maintaining normal intestinal function.

This invention relates to a drug delivery composition, a self-emulsifying composition thereof and a method for producing the same for oral administration to enhance the normalisation of fatty liver. More particularly, the self-emulsifying composition comprises a non-ionic surfactant, a co-surfactant, vegetable oil and active ingredients comprising phospholipids, tocotrienols and tocopherols. The composition aforementioned works synergistically to provide an effectively delivered micronutrients and vitamins to the liver and also facilitate hepatic regeneration thereof.

A filtration mechanism for phospholipid production includes a base frame, a fixing component on the base frame, a limiting component on the fixing component, and a transmission component on the base frame. This utility model, as a filtration mechanism for phospholipid production, uses a rotating frame driven gear transmission system through the fixing component to precisely convert rotational motion into synchronous rotation of a double-ended threaded rod. Linear guidance by a guide rod causes coordinated relative displacement of symmetrically arranged clamping frames, forming a stable and reliable clamping force to firmly fix the filter screen to the filter housing. This design abandons the traditional bolt fixing method, achieving filter screen installation and removal with single-handed operation through an optimized mechanical transmission chain, significantly improving maintenance efficiency. The gear-threaded rod composite transmission mechanism ensures uniform distribution of clamping force, completely avoiding localized stress concentration while guaranteeing reliable fixation. The symmetrical clamping structure ensures balanced force on the filter screen.

The application provides a high-stability DHA emulsion and a preparation method thereof. The high-stability DHA emulsion comprises the following raw materials in parts by mass: 20-40 parts of DHA algal oil; 1-3 parts of phospholipid; 2-5 parts of emulsifier; 0.05-0.3 parts of fat-soluble antioxidant; 0.05-0.3 parts of water-soluble antioxidant; 2-3 parts of lactoferrin-reducing oligosaccharide conjugate; 0.5-2 parts of polysaccharide; and 55-75 parts of water. The high-stability DHA emulsion can be used as a nutritional fortifier for milk and dairy products, beverages and formula food.

The application provides a water-triggered self-assembly gel, a use method and application thereof. The water-triggered self-assembly gel comprises 5-aminolevulinic acid hydrochloride 5-15 wt%, phospholipid 5-10 wt%, anionic glycolipid surfactant 0.2-0.8 wt%, polyethylene glycol 50-60 wt%, propylene glycol 20-27 wt% and antioxidant 0.05-0.1 wt%, and water <0.1 wt%; the mass ratio of the polyethylene glycol and the propylene glycol is (2-2.5):1. The water content of the water-triggered self-assembly gel is extremely low, so that the stability is good and the storage is easy; when used, the water-triggered mechanism of transdermal water loss can be used to actively extract water from the stratum corneum, the phase transition of the phospholipid from the disordered lamellar phase to the liposome is quickly completed, the nanometer liposome with uniform particle size and high encapsulation efficiency is spontaneously formed, the 5-ALA hydrochloride is wrapped, and the 5-ALA hydrochloride can be efficiently delivered to the deep layer of the skin.

The application discloses a kind of modular serum-free cell culture medium and its application, belong to biomedical technology field.The culture medium includes: epidermal growth factor, recombinant insulin, cholesterol-phospholipid nanoparticles, beta-mercaptoethanol, pluronic F-68, glucose / glutamine, trace element mixed solution.The modular serum-free cell culture medium provided by the application can not only meet the large-scale culture of stem cells, but also be used for the industrial production of in vitro diagnostic reagent raw materials such as recombinant antigen, viral vector, recombinant protein and monoclonal antibody, with wide cell adaptability, low production cost, significant economy, stable process, and strict requirements of FDA / EMA for biological products.

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

This invention provides a mutant phospholipase D for the efficient synthesis of phosphatidylserine, its products, and applications. The mutant is based on phospholipase D with the amino acid sequence shown in SEQ ID NO.1, obtained by mutating one or more of five sites at positions 87, 136, 189, 379, and 458 of this amino acid sequence. The amino acid mutation types at these sites include: L87E, M136G, I189R, E379L, and A458Y. This invention provides both a mutant phospholipase D and its applications. This mutant phospholipase D exhibits improved thermostability and catalytic activity, enabling the catalytic preparation of phosphatidylserine series products. The phosphatidylserine series products prepared using this enzyme can effectively alleviate metabolic syndrome induced by antipsychotic drugs.

The application belongs to the field of extracellular vesicle enrichment materials, and relates to a preparation method and application of an extracellular vesicle enrichment material. Polystyrene (PS) nanospheres are used as templates to in-situ grow ZIF-8 in ordered cavities by a multi-phase nucleation method induced by double solvents, to form a single crystal SOM-ZIF-8 material with a directional and ordered macroscopic microporous structure, and to make the whole material have an amino active functional group by exchanging 3-amino-1,2,4-triazole with 2-methyl imidazole ligands in the SOM-ZIF-8 material. Finally, SOM-ZIF-8-NH2 material is acylated with a phospholipid probe to obtain SOM-ZIF-8-phospholipid probe material. The prepared SOM-ZIF-8-phospholipid probe material can efficiently and quickly enrich EVs from a biological sample. In addition, biological analysis of proteins and nucleic acids in EVs by combining molecular biology experiments and micro ribonucleic acid sequencing (miRNA-seq) and other technologies can find potential cancer biomarkers, which provides a reliable method for downstream functional analysis of EVs and is conducive to in-depth study of the biological function of EVs in a biological sample.

The application relates to a preparation method of immobilized phospholipase D and a preparation method of phosphatidylserine thereof, and belongs to the technical field of biotechnology.The preparation method of the immobilized phospholipase D comprises the following steps: (1) mixing mesoporous molecular sieve SBA-15 and a sodium acetate buffer solution to obtain a mixed solution; (2) mixing the mixed solution with an enzyme solution, oscillating, centrifuging, taking the precipitate, cleaning and freeze-drying to obtain the immobilized phospholipase D.The application improves the catalytic performance and stability of PLD by fixing PLD through the immobilization technology, realizes the reusability of PLD, and provides technical support for the industrialized production of the immobilized enzyme of PS.The preparation of phosphatidylserine by the immobilized PLD is optimized, and the optimal process condition of the preparation of PS by the immobilized PLD is obtained.The application further discloses a potential molecular mechanism of PS in treating AD, and provides a theoretical basis for the further clinical application of PS.

Oil-in-water emulsion and its use in cosmetics The present invention relates to a composition in the form of an oil-in-water emulsion, particularly for cosmetic use, especially for the care of keratinous materials, comprising: - at least one oil phase, - at least one emulsifier comprising at least one phospholipid, - at least one hydrophilic gelling agent, - at least one glyceryl ester, and - at least one linoleic acid.

This invention provides an application and method of lysophosphatidylcholine (LPC) in promoting early development of reconstructed embryos, belonging to the field of embryo engineering technology. The invention first prepares reconstructed embryos, then adds lysophosphatidylcholine to the culture system for culturing. Lysophosphatidylcholine effectively promotes early development of the reconstructed embryos; at an optimal concentration of 5 μM, the blastocyst development rate increases to 24.56%, providing a novel and operable biochemical intervention target to solve the problem of low reconstructed embryo culture efficiency. This invention not only fills the gap in our understanding of the mechanism of action of LPC in this field, but also achieves controllability and reproducibility of the technical solution through precise definition of the optimal concentration. It provides key parameters for establishing standardized culture procedures and provides a more solid foundation for downstream applications such as developmental biology research and disease model construction that rely on high-quality reconstructed embryos, significantly accelerating the process of related scientific discovery and application development.

The application discloses an obstetric antiphospholipid syndrome risk prediction method and system, relates to the technical field of medical information processing, and comprises the following steps: acquiring clinical sign data, laboratory specific antibody index data, previous medical history data and diagnosis results of a pregnant woman, preprocessing to obtain a standardized data set; extracting time sequence combination features and interaction features to form a fusion feature set; calculating feature correlation intensity values to screen a core feature subset; extracting training features by using the core feature subset, training a risk prediction model; extracting prediction features of a pregnant woman to be predicted and adjusting weights according to a gestational stage; inputting the weighted features into the model to generate a risk score, determining a risk grade and outputting early warning information. Through multi-dimensional data analysis and dynamic weight adjustment, the application realizes accurate prediction and timely early warning of obstetric antiphospholipid syndrome risk.

The application discloses a method for regulating virus proliferation in rice planthopper. The method regulates the content of 3,5-bisphospho phosphatidylinositol (PI(3,5)P2) to regulate the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf virus in the body of white-backed planthopper. The PI(3,5)P2 inhibitor or dsRNA of PI(3,5)P2 synthesis enzyme gene PIKfyve is applied by microinjection, drop or spraying to reduce the content of PI(3,5)P2 in the body of Laodelphax striatellus and white-backed planthopper, thereby inhibiting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf virus in the body of white-backed planthopper. The PI(3,5)P2 homolog PI(3,5)P2-diC8 is applied to Laodelphax striatellus to increase the content of PI(3,5)P2, thereby promoting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus. The application provides a new method for controlling virus diseases transmitted by vector insects.

A method for increasing the intradermal retention amount of a polypeptide in a cosmetic product, and an anti-aging cosmetic product. The method for increasing the intradermal retention amount of a polypeptide in a cosmetic product comprises: mixing a polypeptide with water to prepare an aqueous phase mixture; mixing a phospholipid, grease and an antioxidant to prepare an oil phase mixture; and dropwise adding the aqueous phase mixture to the oil phase mixture to form a viscous reverse micelle structure. The anti-aging cosmetic product comprises the viscous reverse micelle structure formed from a polypeptide raw material.

The present application provides a kind of animal-plant mixed light cream and its preparation method, the light cream includes phospholipid 0.005-0.015% according to mass percentage, Tween 80 emulsifier 0.004-0.02%, mono, double glycerin fatty acid ester 0.001-0.009%, sodium stearoyl lactate 0.03-0.04%, sodium caseinate 0.03-0.04%, polyglycerin fatty acid ester 0.02-0.04%, also includes vegetable oil, raw milk, light cream.The animal-plant mixed light cream provided by the present application has the technical effect that the whipping rate is higher.

Provided is a simple and specific means for detecting Listeria monocytogenes and / or Listeria ivanovii. A culture medium for detecting Listeria monocytogenes and / or Listeria ivanovii contains the following components (A), (B), and (C): (A) a chromogenic substance that develops color by being decomposed with β-glucosidase or a fluorogenic substance that emits fluorescence; (B) a chromogenic substance that develops color by being decomposed with phosphatidylinositol-specific phospholipase C or a fluorogenic substance that emits fluorescence; and (C) a sugar.

Hair treatment preparations which comprise two superimposed layers and a common interface and contain at least one C9-C12 alkane in the oil phase (II) and at least one cationic phospholipid in the water phase (I) are suitable for hair care, in particular for improving the detanglability, combability, smoothness, shine and frizz of hair.

The present application relates to the application of phospholipids in regulating angiogenesis, and the present application applies various phospholipids and products thereof to a zebrafish model to evaluate the influence of the phospholipids on the damage of intersegmental vessels (ISVs) induced by vascular endothelial cell growth factor receptor inhibitor (PTK787) and the growth of subintestinal vessels (SIVs) in the zebrafish, and it is found for the first time that phospholipids from different sources and in different forms can reverse the damage of ISVs induced by PTK787, significantly promote the growth of SIVs in the zebrafish, and increase the length of the generated blood vessels to different degrees, and have small toxic and side effects; the present application can provide candidates for preparing drugs for vascular diseases, and has a good application prospect.

This invention belongs to the pharmaceutical field and specifically relates to magnesium hydroxide nanoparticles for treating joint pain and a method for preparing the same. The preparation method is as follows: a soluble magnesium salt is dissolved in purified water to obtain a magnesium salt solution; a soluble metal hydroxide is dissolved in purified water to obtain a metal hydroxide solution; a phospholipid is dissolved in an organic solvent to obtain a phospholipid solution; the phospholipid solution is removed by rotary vapor deposition to remove the organic reagents; after a thin film is formed, the magnesium salt solution is added to hydrate it to obtain a hydrated solution; the metal hydroxide solution is added to the hydrated solution to perform nano-processing; and the process is completed. The preparation method is simple and easy to control, and magnesium hydroxide nanoparticles can be prepared using only magnesium salt, hydroxide, and phospholipid. Verified by rat studies, after preparing magnesium salt into nano-level magnesium hydroxide using pharmaceutical formulation technology, it has joint analgesic effects, the duration of analgesia is significantly extended, the frequency of administration is greatly reduced, and thus the patient's suffering can be reduced.

Provided are a skin care composition, a method for preparing same, and a system for skin care. The skin care composition comprises an active ingredient and a carrier. The active ingredient comprises a whitening ingredient, an anti-inflammatory ingredient, and a soothing ingredient. The carrier comprises a phospholipid ingredient, an emulsifier, a non-aqueous solvent, and water. The whitening ingredient comprises glabridin, nicotinamide, and nonapeptide-1. The anti-inflammatory ingredient comprises palmitoyl tripeptide-8. The soothing ingredient comprises acetyl dipeptide-1 cetyl ester. The carrier comprises a liposome particle. The liposome particle at least partially encapsulates the active ingredient and has an average particle size of less than or equal to 500 nm. The skin care composition can achieve a significant whitening effect.

A method for preparing photophosphorylated nanorobots for targeted therapy of myocardial injury is disclosed. This invention aims to construct self-driven nanorobots with near-infrared light response capabilities to achieve targeted in-situ ATP supply and repair of damaged myocardium. The nanorobots of this invention are constructed based on supramolecular assembly and phospholipid phase separation principles, incorporating F-containing... o F l The pigment clusters of the ATPase and photophosphorylated protein system were co-extruded with synthetic phospholipids to form a hybrid structure with near-infrared light responsiveness. Under 850 nm near-infrared light activation, F… o F l -ATPase synergistically rotates and synchronously generates ATP, while simultaneously enhancing the directional movement capability of the nanorobots. These nanorobots can actively accumulate in areas of myocardial injury in vivo, achieving efficient lysosomal escape and deep tissue penetration, and providing in-situ ATP supply within cells to increase energy levels, restore mitochondrial function, and complete myocardial repair. This invention belongs to the field of nanorobots.