1264 results about "Phospholipin" patented technology

The invention relates to a stem cell bioactive nano-vesicle with efficient inflammation regulation and tissue repair effects, the average particle size of the nano-vesicle is 100-350nm, the polydispersity index (PDI) is 0.05-0.40, and the expression rate of phosphatidylserine (PS) on the surface of the nano-vesicle is greater than 40%. According to the preparation method, stem cells are subjected to apoptosis induction, and then a programmed serial extrusion process is adopted to prepare the nano-vesicles. The nano vesicles are moderate in particle size, uniform in distribution and high in production efficiency, the preparation process is easy to operate, the quality is stable and controllable, and the nano vesicles have excellent technical effects in the aspects of repair and regeneration of inflammation injury type tissues and organs.

The present application relates to a kind of dihydrocarbylimidazole biomimetic lipid compound and its preparation method and application, dihydrocarbylimidazole biomimetic lipid compound structure imitates natural phospholipid design, modification is not less than 10 carbon atoms alkyl on the 4th and 5th of imidazole, and modification is different alkyl chain length primary amine on 2nd position;Dihydrocarbylimidazole biomimetic lipid compound is mixed with therapeutic drug and other adjuvant, can be prepared to be loaded in the lipid nanoparticle of therapeutic drug, can be used as drug delivery system for the preparation of brain-targeted drug.Dihydrocarbylimidazole biomimetic lipid has the function of dynamic control blood-brain barrier, can realize the reversible opening of blood-brain barrier;Formed drug-loaded lipid composition can cross blood-brain barrier and better realize the brain-targeted delivery of loaded therapeutic drug;Drug-loaded lipid composition consisting of dihydrocarbylimidazole biomimetic lipid belongs to a new generation of biomimetic nanomedicine carrier, provides new strategy for realizing the brain-targeted delivery of different kinds of drugs.

The invention provides a Yarrowia lipolytica engineering strain for producing cocoa butter equivalent and a construction method and application thereof, and relates to the technical field of biology, the construction method comprises the following steps: inserting an anti-translation PFAD2 promoter into a Yarrowia lipolytica chassis strain to adjust the composition of target fatty acid, so that the contents of palmitic acid and stearic acid are respectively increased to 27.3% and 19.8%; the contents of palmitoleic acid and oleic acid are reduced to 4.5% and 36.8%; the twelve-site fatty acid desaturase gene FAD2 is knocked out, and linoleic acid is completely eliminated; a plant-derived diacylglycerol acyltransferase gene DGAT or phospholipid is introduced; the diacylglycerol acyltransferase gene PDAT is used for synthesizing cocoa butter equivalent (CBE). The yield of cocoa butter equivalent of the constructed yarrowia lipolytica engineering strain finally reaches 1.00 + / -0.09 g / L and accounts for 30% of the total fat content. The technical problem of low cocoa butter equivalent production efficiency of engineering modified yeast in the prior art is solved.

The invention belongs to the field of enzymatic preparation of structural phospholipids, and particularly relates to a method for preparing phosphatidylserine through interfacial enzyme catalysis. A series of high-activity immobilized phospholipase are prepared by innovatively adopting bifunctional group modified hollow mesoporous silica microspheres as a carrier and adsorbing immobilized phospholipase D. The catalyst has the functions of a catalyst and an emulsifier, and a Pickering emulsion interfacial enzyme catalysis system can be efficiently constructed. In the system, the phosphatidylcholine and the serine are efficiently converted into the phosphatidylserine through a phosphatidyl transfer reaction, the conversion rate of the phosphatidylserine within 20 minutes can reach 85%-95%, and the catalytic efficiency reaches up to 372 mmol / (g.h). In addition, after the catalyst is recycled for 10 times, the conversion rate is still kept at 70.9%, and the catalyst shows excellent stability and reusability. The method has the remarkable advantages of being mild in reaction condition, high in catalytic efficiency, capable of being repeatedly used and the like, and a new technical approach is provided for industrial production of phosphatidylserine.

The invention provides a compound phospholipid gel sustained release preparation and a preparation method thereof. The preparation mainly comprises a local anesthetic, a non-steroidal anti-inflammatory drug, phospholipid and water for injection. The preparation can be injected around a surgical incision or directly instilled or coated at the surgical incision to achieve the purpose of long-acting analgesia, and is used for postoperative analgesia.

The invention provides an mRNA (messenger ribonucleic acid) vaccine and application of the mRNA vaccine in preparation of a medicine for treating tumors. The vaccine consists of ionizable lipid, cholesterol, phospholipid, a PEG (Polyethylene Glycol) derivative and mRNA (Messenger Ribonucleic Acid) containing a gene sequence for coding norovirus S protein fusion tumor antigen or a gene sequence for coding tumor antigen fusion ferritin. According to the mRNA vaccine designed by the invention, the antigen expression quantity is increased, a nanocrystallization antigen structure is formed to promote antigen processing and presentation, strong anti-tumor cell immune response is stimulated, and a tumor local microenvironment is effectively remodeled, so that a remarkable tumor inhibition effect is achieved.

The invention relates to bionic skin barrier cream with a three-layer phospholipid structure and a preparation method of the bionic skin barrier cream, and belongs to the technical field of cosmetics, the bionic skin barrier cream comprises the following raw materials: lecithin, wax ester, triglyceride, composite ceramide, phytosterol oleate, squalane, an antioxidant, polyol, composite amino acid, pyrrolidone carboxylic acid, urea and arginine. A stabilizing agent, a treating agent and the balance of deionized water. The bionic skin barrier cream prepared by the invention has a three-layer phospholipid structure, and compared with a double-layer phospholipid structure in the market, the firmness and hydrophobicity of the double-layer structure are further improved by the added third-layer structure, so that the prepared bionic skin barrier cream has outstanding waterproof, sweat-resistant and light pollution-resistant effects; in addition, the wax ester, the triglyceride, the phytosterol oleate, the pyrrolidone carboxylic acid and the urea selected in the invention have a synergistic effect, and can generate excellent moisturizing and repairing effects and certain protection effects.

The invention belongs to the technical field of bionic nano-drugs, and particularly relates to a bionic vesicle preparation as well as a preparation method and application thereof. The bionic vesicle preparation disclosed by the invention is prepared from the following raw materials: mesenchymal stem cell outer vesicles, nintedanib and DSPE-PEG-HA (hyaluronic acid modified pegylated phospholipid); the nintedanib is loaded in the mesenchymal stem cell outer vesicle, and the DSPE-PEG-HA is coupled to the surface of the mesenchymal stem cell outer vesicle. The bionic vesicle preparation disclosed by the invention can be used for directly delivering a drug to a pulmonary fibrosis injury part through inhalation type drug delivery, and acting on a focus part at a higher local concentration, meanwhile, the exposure of the drug in whole-body circulation is remarkably reduced, the occurrence of side effects is reduced, and the specific targeting intervention and regulation on fibroblasts are realized.

The invention discloses a compound composition for improving sleep disorders, which is characterized by comprising the following components: an inhibitory neurotransmitter component, melatonin and a nano-lipid carrier, the nano-lipid carrier comprises phospholipid and cholesterol, the weight ratio of phospholipid to cholesterol is (1-6): 1, the weight ratio of phospholipid to cholesterol is (1-6): 1, and the weight ratio of melatonin to cholesterol is (1-6): 1. The inhibitory neurotransmitter component is wrapped in the nano-lipid carrier to form lipid nanoparticles, and the weight ratio of the inhibitory neurotransmitter component to the nano-lipid carrier is 1: (1-15). The compound composition can be prepared into a compound oral mucosa preparation, can be rapidly disintegrated when encountering saliva in the oral cavity, is good in patient compliance and small in side effect, all the components play a synergistic effect from different mechanisms through multiple target points, and the compound oral mucosa preparation can improve the sleep quality, prolong the sleep time and shorten the sleep latency; and patients with sleep disorders are helped to improve insomnia from the pathologic root.

The invention belongs to the technical field of biological medicine, and discloses a novel adjuvant recombinant herpes zoster vaccine and a preparation method thereof. The vaccine is composed of gE-gD protein and a liposome adjuvant, the liposome adjuvant is prepared by the following steps: co-dissolving 3D-MLA, phospholipid-containing components and steroid components in an organic solvent to form a film, hydrating and homogenizing with PBS, finally adding QS-21, and fixing the volume with PBS. According to the process, enhanced components are uniformly and stably anchored in a membrane phase micro-domain, and the particle structure is controllable. Under the same antigen dosage and detection caliber, the D28 GMC of the vaccine is obviously improved, and more sufficient and stronger primary humoral immune response is embodied.

The invention relates to a nano-liposome preparation, which is characterized in that a liposome prepared from distearoyl phosphatidyl glycerol (DSPG), cholesterol and Biotin-PEG3000-cholesterol is used as a carrier, the surface of the nano-liposome preparation is modified with an ICAM-1 monoclonal antibody, and the nano-liposome preparation is loaded with an AIE photosensitizer and Nicodil. The nano-liposome prepared by the invention has targeting and responsive drug delivery to infected lesions, the enrichment of drugs at bacterial infected parts is improved, nano-system components can be cut by phospholipase in an infected microenvironment, responsive release of the drugs at the bacterial infected parts can be realized, and the treatment effect is improved. According to the invention, a hydrophobic shell layer and a hydrophilic core of the liposome are utilized, and hydrophobic NO donor molecules and a hydrophilic AIE photosensitizer are loaded at the same time, so that synergistic treatment of bacterial infection by a photodynamic therapy and a gas therapy is realized, and a better killing effect on drug-resistant bacteria is achieved.

The invention belongs to the technical field of liquid chromatography-mass spectrometry detection pretreatment, and particularly relates to a pretreatment method for liquid chromatography-tandem mass spectrometry analysis of pesticide and veterinary drug residues in serum, which comprises the following steps: (S1) carrying out heating reaction on Sc (III) salt, Cu (II) salt and 1H-pyrazole-4-formic acid (H2PyC) in a polar aprotic solvent, and cooling to obtain a green crystal, namely MOF-919; the chemical formula of the MOF-919 is [Sc < 3 > (mu3-O) (OH) < 3 >] [Cu3 (mu3-O) (mu-PyC) < 3 > (H2O) < 6 >] < 2 >; (S2) mixing the serum sample with an acetonitrile solution containing formic acid, carrying out ultrasonic treatment, vortex oscillation and centrifugation, taking supernate, and diluting the supernate with an aqueous solution containing formic acid to obtain a to-be-purified solution; and (S3) adding MOF-919 into the to-be-purified liquid, mixing, performing ultrasonic treatment, performing vortex oscillation, centrifuging, and taking supernate, thereby completing the pretreatment of the to-be-purified liquid. According to the method, the MOF-919 is used for adsorbing chemical pollutant residues in serum, especially phospholipids, so that the purpose of removing the chemical pollutant residues in blood is achieved. The method is simple and convenient to operate, small in organic solvent consumption and good in purification effect, and can be widely applied to residue analysis of chemical pollutants in serum.

The invention discloses an antioxidant vitamin A ester microcapsule and a preparation method thereof, vitamin A ester is prepared into compact solid powder, external oxygen is effectively isolated, the solid powder is used as a core material, one or two of hydrogenated oil or monoglyceride is / are mixed with phospholipid as a wall material, and the antioxidant vitamin A ester microcapsule is prepared. The core material and the wall material are mixed and then subjected to high-speed dispersion and spray condensation treatment to obtain the vitamin A ester microcapsule, so that the storage stability of the vitamin A ester is greatly improved; meanwhile, the preparation process is simple, and large-scale production is facilitated. The encapsulation efficiency of the antioxidant vitamin A ester microcapsule prepared by the method is more than 90%. And in 35-day accelerated storage test evaluation at 37 DEG C, the retention rate is 80% or above and is obviously higher than the retention rate of the raw materials.

The invention discloses a hair follicle targeting nano-liposome anti-hair loss and hair growth compound preparation. The compound preparation is prepared from the following raw materials in parts by mass: 70 to 85 parts of bionic liposome carrier and 15 to 30 parts of active ingredients, the bionic liposome carrier is prepared from 50 to 60 parts of egg yolk lecithin, 15 to 20 parts of pH sensitive phospholipid DOPE, 10 to 15 parts of cholesterol and 5 to 10 parts of mesenchymal stem cell exosome membrane protein; the active ingredients comprise 5 to 10 parts of minoxidil, 3 to 5 parts of caffeine, 0.5 to 1 part of miR-218-5p, 1 to 2 parts of biotin and 2 to 3 parts of quercetin; the average particle size of the nano-liposome formed by the bionic liposome carrier is 120-180nm, and the polydispersity index (PDI) is less than or equal to 0.15. The invention relates to the technical field of biological medicines, in particular to a hair follicle-targeted nano-liposome anti-hair loss and hair growth compound preparation, which has the following advantages due to the adoption of the technical scheme: accurate targeted delivery is realized, and the treatment safety and accuracy are improved; intelligent response release is achieved, and the local microenvironment of hair follicles is optimized; the multi-target synergistic effect breaks through the limitation of single treatment.

The invention discloses a lipophilic liposome containing polydeoxyribonucleotide as well as a preparation method and application of the lipophilic liposome. The lipophilic liposome comprises the following components in parts by weight: 0.001-3 parts of polydeoxyribonucleotide; 0.5 to 3 parts of phospholipid; 0.1-2 parts of a cationic emulsifier; 0.1-3 parts of an anionic emulsifier; 40 to 70 parts of polyol; the total amount is 100 parts. According to the invention, an emulsifier system formed by combining lecithin with anionic and cationic compound surfactants is adopted, so that the transdermal absorption of PDRN is facilitated. The lipophilic liposome containing polydeoxyribonucleotide prepared by the invention can effectively resist nuclease degradation, prolong the acting time of PDRN in skin, and improve the skin anti-aging and repairing effects of the liposome. The anti-aging effect of the active ingredients is improved, and the bioavailability of the active ingredients is improved. The cosmetic composition can be widely applied to cosmetic formulas of various dosage forms.

The present disclosure provides amino acid sequences (e.g., antibodies) capable of binding to glypican 3 protein (GPC3). The present disclosure relates to antigen binding fragments of GPC3 antibodies; conjugates of a GPC3 antibody, including an immunoconjugate; bispecific antibodies comprising a GPC3 antibody and related compositions and formulations. The disclosure also relates to methods of treating GPC3-related diseases, disorders and conditions, including cell proliferative diseases, such as cancer, more particularly liver cancer, using these sequences, such as GPC3 antibodies and antigen-binding fragments thereof.

The invention discloses a metal polyphenol nano material based on chlorogenic acid and a preparation method and application thereof.The preparation method comprises the steps that chlorogenic acid and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 are mixed and dissolved in an anhydrous tetrahydrofuran solvent, and a first mixed solution is prepared; adding a FeCl3 solution into the first mixed solution until a clear and transparent system is formed, so as to obtain a second mixed solution; and removing the anhydrous tetrahydrofuran solvent of the second mixed solution to obtain the metal polyphenol nano material (CA-Fe NPs) based on chlorogenic acid. The metal polyphenol nano material has excellent photothermal conversion performance, and can be applied to in-vitro antibiosis, antioxidation, anti-inflammation and wound healing promotion.

The invention discloses a phosphatidylcholine gold nano CT (Computed Tomography) probe as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The preparation method comprises the following steps: step S1, preparing a phosphatidylcholine ligand NH2-MPC; step S2, synthesis of a gold nanocluster GS-Au25 is carried out; and S3, the GS-Au25 is modified with a PC ligand, and the PC-Au25 is prepared. The ultra-small gold nano CT probe PC-Au25 with an Au25 gold nano cluster as a core and phosphatidylcholine PC as a surface ligand is obtained by optimizing and screening the core size and the surface ligand property of ultra-small gold nano particles. Wherein the 2-methacryloyloxyethyl phosphorylcholine is used as a PC ligand for the first time to modify the surfaces of the ultra-small gold nanoparticles, so that the surface modification of the ultra-small gold nanoparticles is realized, and the ultra-small gold nanoparticles can be efficiently and freely filtered through the kidney. The CT probe can be applied to the determination of the glomerular filtration rate through a CT imaging or blood test method.

The invention provides a target antibody or an antigen binding fragment thereof and application thereof, and belongs to the field of antibodies. Wherein the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR, and the HCDR comprises at least one selected from SEQ ID NO: 1-18 or an amino acid sequence having at least 80% homology with the HCDR. The antibody or the antigen-binding fragment thereof can bind to TIM-1 protein with high specificity and affinity, increase the blocking of TIM-1 activity so as to enable the TIM-1 to interact with ligands such as phosphatidylserine, TIM-4 and galectin-9, and enhance immune response.

The invention provides electroactive nano short fibers for osteoarthritis cartilage repair and a preparation method thereof, the preparation method comprises the following steps: preparing a nano fiber membrane from a composite material of gelatin, polylactic acid and glycine crystals through electrostatic spinning, and preparing the obtained nano fiber membrane into short fibers; performing freeze drying on the short fibers, and then performing heating treatment to obtain a short fiber stent; the short fiber support is smashed and reacts with graphene oxide, modified graphene oxide short fibers are obtained, and then the modified graphene oxide short fibers are soaked in a mixed solution of alkaline dopamine and phosphatidylcholine for a reaction. The electroactive nano short fiber provided by the invention is low in friction coefficient, the expression quantity of Sirt1 in OA cartilage cells can be remarkably increased under ultrasonic stimulation, the ATP (adenosine triphosphate) productivity can be remarkably improved, release of inflammatory factors and degradation of extracellular matrixes can be inhibited, and meanwhile, a guarantee is provided for updating of a hydrated lubricating layer.

The invention relates to a composition with antioxidant and brightening effects and capable of delaying skin saccharification as well as a preparation method and application of the composition, and belongs to the field of cosmetics. The composition comprises an ormosia henryi bark extract, tetrahydrocurcumin, a mirabilis jalapa extracting solution, a surfactant, polyol, phospholipid and a solvent. The composition provided by the invention has good illumination stability and high-temperature stability, and the composition provided by the invention is high in bioavailability, and has good antioxidant and brightening effects and lipofuscin and RAGE level inhibition effects.

The invention provides a cobalt monatomic nano-enzyme system modified by phospholipid polyethylene glycol carboxyl. The cobalt monatomic nano-enzyme system comprises a cobalt monatomic nano-enzyme composite core and phospholipid polyethylene glycol carboxyl compounded on the surface of cobalt monatomic nano-enzyme, the cobalt monatomic nano-enzyme composite core comprises a cobalt monatomic nano-enzyme and ginsenoside loaded on the cobalt monatomic nano-enzyme. The invention also provides a bifidobacterium synergetic cobalt monatomic nano-enzyme system which is formed by combining cobalt monatomic nano-enzyme with ginsenoside Rb1 and further delivering the cobalt monatomic nano-enzyme and ginsenoside Rb1 through bifidobacterium bifidum for targeted therapy of enteritis. The combination of Co SA and Rb1 can enhance the anti-inflammatory efficacy by adjusting a key immune signal pathway, and bifidobacterium bifidum can ensure targeted delivery of microbiota and promote intestinal barrier repair. The synergistic three-component strategy integrating monatomic nano-enzyme antioxidation, natural drug immunoregulation and probiotic targeting is provided, and a promising treatment platform is provided for accurate intervention of IBD.

The invention relates to a serum liquid chromatography-tandem mass spectrometry pretreatment method, which comprises: (S1) mixing a serum sample and a formic acid-containing acetonitrile solution, carrying out ultrasonic treatment, vortex oscillation and centrifugation, taking the supernatant, and diluting with a formic acid-containing aqueous solution to obtain a liquid to be purified; (S2) mixing the to-be-purified liquid with PCN-777, performing ultrasonic treatment, vortex oscillation and centrifugation, and taking supernate to obtain to-be-detected liquid; the PCN-777 is a C24H23N3O16Zr3 phospholipid removal material, and the PCN-777 is a C24H23N3O16Zr3 phospholipid removal material, and the PCN-777 is a C24H23N3O16Zr3 phospholipid removal material. According to the present invention, the serum ultra-high performance liquid chromatography-tandem mass spectrometry pretreatment method is developed by using the known MOFs material PCN-777, such that different types of phospholipids in the serum can be specifically, rapidly and effectively removed, the efficient recovery and detection of pesticide and veterinary drugs can be achieved, and the interference of phospholipids can be avoided.

The invention relates to the technical field of biological medicines, in particular to an iRGD targeted and anti-fibrosis small molecule dual-modified exosome as well as a construction method and application thereof. The exosome utilizes the specific binding penetration enhancement effect of iRGD (the amino acid sequence is CRGDKGPDC) and a fibroblast high-expression integrin receptor, so that the enrichment efficiency of the exosome at a focus part is improved; the anti-fibrosis micromolecule Let-7a-5p can inhibit a TGF-beta signal channel by blocking Smad2 / 3 phosphorylation, and the anti-fibrosis micromolecule Let-7a-5p and the anti-fibrosis microRNA carried by the exosome generate a synergistic anti-fibrosis effect; the exosome phospholipid bilayer can also protect Let-7a-5p from enzymolysis and prolong the half-life period, and iRGD modification can reduce the non-targeted uptake rate. An animal model verifies that the double-modified exosome has a good skin fibrosis resisting effect, and side effects such as liver and kidney function damage are not found. The invention provides an effective and safe treatment scheme for resisting skin fibrosis.

Provided herein are lipid nanoparticles composition comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide. Further provided are lipid nanoparticle compositions comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide selected from: N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C8 PEG5000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]}(C8 PEG2000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C8 PEG750-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C16 PEG5000-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (C16 PEG200-Ceramide), and N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C16 PEG750-Ceramide) (iii) a phospholipid, and (iv) a permanently cationic lipid, an anionic lipid, or a second ionizable cationic lipid separate from the first ionizable cationic lipid.

The invention discloses a preparation method and application of a photo-thermal biosensor for tumor cell quantification and vitality dual-index liquid biopsy. Phosphatidylserine (PS) is exposed by enriching and cracking tumor cells, is identified and combined by an aptamer Apt2, and is driven to be separated from Apt2-Cu2-xTe NSs; after magnetic separation, separated supernatant is subjected to photo-thermal detection; the photo-thermal signal intensity is positively correlated with the number of cells, so that quantitative detection is realized; during cell apoptosis, PS is turned out of the membrane, Apt2-Cu2-xTe NSs is combined with the surface of the membrane, separated supernatant is subjected to photo-thermal detection after magnetic separation, and the photo-thermal signal enhancement amplitude is in negative correlation with cell activity. The platform has the advantages of POCT characteristics, low cost and portability, and a new tool is provided for precise tumor diagnosis and treatment.

The invention discloses a near-infrared light nano probe as well as a preparation method and application thereof. The preparation method of the near-infrared nano probe comprises the following steps: dissolving polylactic acid-co-acetic acid in an acetonitrile solution, and adding lecithin and 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidyl glycol to obtain a solution A; dissolving indocyanine green in an ethanol solution to obtain a solution B; and mixing the solution A and the solution B, and carrying out ultrasonic treatment to obtain the near-infrared nano probe. The near-infrared light nanoprobe can be used for imaging diagnosis of acute kidney injury, and effectively solves the problems of long diagnosis time, poor stability, high in-vivo clearance rate, low cell absorption rate and the like. Compared with existing ICG nano-particles, the ICG nano-particles are high in biological tissue penetrability, high in resolution ratio, low in phototoxicity and good in fluorescence imaging effect in an acute kidney injury model and have wide application prospects in AKI clinical diagnosis.

The present invention provides a high brain targeting, safe and noninvasive nasal-brain delivery preparation, which is characterized in that the nasal-brain delivery preparation comprises nimodipine, a good solvent, a diluent and an absorption enhancer, the good solvent is diethylene glycol monoethyl ether, and the absorption enhancer is phospholipid. The nasal-brain delivery preparation is prepared from a good solvent, a diluent, an absorption enhancer and the like according to a specific proportion. Nimodipine serving as an active ingredient is enriched in cerebrospinal fluid and brain tissues through a specific upper nasal meatus administration technology, so that the treatment effect of nimodipine is achieved; and meanwhile, the blood entering proportion is reduced, so that the cardiovascular toxicity and hepatotoxicity are reduced. In addition, the preparation formula has no irritation to local parts of the nasal cavity, and the compliance of a patient is improved.

Described herein are compositions, kits, and methods for potent delivery to a cell of a subject. The cell can be of a particular cell type, such as a basal cell, a ciliated cell, or a secretory cell. In some cases, the cell can be a lung cell of a particular cell type. Also described herein are pharmaceutical compositions comprising a therapeutic or prophylactic agent assembled with a lipid composition. The lipid composition can comprise an ionizable cationic lipid, a phospholipid, and a selective organ targeting lipid. Further described herein are high-potency dosage forms of a therapeutic or prophylactic agent formulated with a lipid composition.

The invention discloses a phospholipase A1 mutant and application thereof. According to the invention, PLA1 is modified to obtain a PLA1 mutant, compared with wild PLA1, the PLA1 mutant has the advantages that the optimum temperature is kept unchanged, the PLA1 mutant has higher phospholipase A1 activity at 40-50 DEG C, the optimum pH is changed from 3 to 8, and the PLA1 mutant keeps high phospholipase activity at the pH of 3-9 and is higher than the phospholipase activity of the PLA1 wild type under the optimum condition; the pH (Potential of Hydrogen) adaptability range of the PLA1 is widened. Therefore, the PLA1 mutant disclosed by the invention has a better application prospect, better meets the requirement on enzyme performance in phospholipid processing, can be used for degumming grease and producing lysophosphatide, and is applied to the fields of biology, food, medicine, beauty, agriculture, industry and the like.