11 results about "Thrombin activity" patented technology

Provided herein are method and kits for identifying thrombus origin based on temporal thrombin activity assay, and use thereof for optimizing post-ischemic event treatment, and further preventing recurrence of secondary ischemic events.

The invention belongs to the technical field of nano preparations, and particularly relates to a hirudin nano drug for relieving chronic asthma and a preparation method thereof.The hirudin nano drug is prepared from, by weight, 0.5-1 part of hirudin prodrug, 10-15 parts of stabilizer, 40-80 parts of freeze-drying protective additive and 1.5 parts of histidine; the hirudin prodrug molecule is composed of hirudin, MMP-9 responsive peptide, ROS responsive thioether bond and hydrophobic hexadecanoic acid, can spontaneously assemble to form a nano-sized prodrug under hydrophilic and hydrophobic effects, has stability in systemic circulation and is targeted and enriched at an asthma airway inflammation part; the hirudin with the thrombin activity inhibition effect is responsively released at the focus, so that the effect and the purpose of slowly treating the asthma are achieved.

The invention belongs to the technical field of anticoagulant drugs, and particularly relates to lumbricus protein with anticoagulant activity as well as a preparation method and application of the lumbricus protein. The lumbricus protein provided by the invention contains at least one of a c71616 protein with an amino acid sequence as shown in SEQ ID No. 1, a c128686 protein with an amino acid sequence as shown in SEQ ID No. 2, a c58213 protein with an amino acid sequence as shown in SEQ ID No. 3 and a c118923 protein with an amino acid sequence as shown in SEQ ID No. 4. The protein can effectively inhibit thrombin activity and interfere formation of fibrin so as to act on a key link of a blood coagulation process. The protein can be obtained through recombinant expression and purification, and has high expression efficiency and stability, so that the protein can be used as an active component to be applied to development of anticoagulant drugs, surface anticoagulant coatings and other products.

The application discloses a preparation device of autologous thrombin, and belongs to the technical field of biomedical engineering, which comprises an activator cylinder and a conducting pipe, a piston head of a piston of the activator cylinder is arranged in an inner container, the inner container is sleeved with an outer cylinder, and a piston seat of the piston is fixedly connected with the outer cylinder. When the outer cylinder is quantitatively moved upward relative to the inner container, the activator in the inner container is pushed by the piston to be added into a test tube through the conducting pipe, so that the activator can be quantitatively added into the test tube, and the added activator can be guaranteed not to be polluted by the outside. The preparation device of autologous thrombin is used to add the activator into centrifuged whole blood to make the whole blood be mixed uniformly, and after standing and contacting, a gel is formed in the mixture, supernatant is formed in the upper part, and the supernatant is sucked to prepare a thrombin solution. The application has the advantages of convenient operation, simple process, effective prevention of external infection, high activity of prepared autologous thrombin and fast gel formation.

This invention discloses an earthworm protein peptide with in vitro thrombin-inhibiting activity and its preparation method, belonging to the field of functional food technology. The method first pre-treats earthworm raw materials to obtain earthworm protein; then, it uses pepsin and alkaline protease for stepwise enzymatic hydrolysis under varying temperature and pH, and ultrafiltration to collect short peptide solutions with a molecular weight below 3 kDa; the short peptide solution is specifically captured using an affinity chromatography material coupled with bovine thrombin, followed by elution, nanofiltration desalting concentration, and spray drying to obtain the final product. This invention fully releases the peptides through a dual enzymatic hydrolysis process and utilizes thrombin affinity chromatography to directionally enrich peptides with specific inhibitory activity, effectively solving the problems of low content and poor targeting of active ingredients in earthworm protein peptides in existing technologies, and significantly improving the purity and in vitro anticoagulant activity of the product.

The invention discloses a method for detecting thrombin activity in a biological product by using a functionalized electrode, and belongs to the technical field of electrochemical detection. Firstly, thrombin specific substrate polypeptide is fixed on the surface of a silk-screen printing gold electrode through an Au-S bond, and the N end of the polypeptide is modified with ferrocene (Fc) to serve as an electroactive signal molecule; then the surface of the electrode is coated with a cellulose acetate membrane (CA) to obtain a functionalized electrode, and the CA membrane can reduce non-specific adsorption and prevent electrode pollution and signal attenuation; dropwise adding a solution containing thrombin onto the functionalized electrode for reaction; and washing the reacted electrode, testing by using a square wave voltammetry, and obtaining the thrombin activity according to the change of the peak current. The method is convenient to operate, high in specificity, short in consumed time and long in storage time of the functionalized electrode, and is an effective means for detecting the thrombin activity in the biological product.

The invention provides a powerful hemostatic adhesive prepared by taking American cockroach dregs as a main raw material. The preparation method comprises the following steps: firstly, extracting and preparing chitin whiskers from waste American cockroach dregs, taking the chitin whiskers as a polymer matrix of the adhesive, taking an aqueous solution of a deep eutectic solvent as a system medium, taking thrombin as a hemostatic functional substance, and improving the overall mechanical properties by gelatin, thereby finally obtaining the biological adhesive with good adhesion and hemostatic performance. According to the product, under the condition that a cross-linking agent does not need to be used, through heating-cooling operation, a polymer skeleton network is constructed through interaction between chitin whiskers and gelatin, a channel is provided for transmitting moisture and nutrient substances to damaged tissues, and the product has degradability while achieving an ideal adhesion effect; the whole system effectively fuses the components, is friendly and easy to prepare, and integrates respective advantages. In addition, the activity and the stability of the thrombin in the eutectic solvent are both in ideal states, so that the adhesive can play a powerful hemostatic role.

The present invention relates to the technical field of rodent pest control. Disclosed are the use of avermectin and a rodenticide formulation. The avermectin has a structure represented by formula (I). The present invention uses a strategy of "new use for old drugs", uses commercially available pesticides as candidate compounds, and uses mouse thrombin as a target to single out pesticide avermectin strongly bound to thrombin, which can activate the activity of mouse thrombin and promote blood coagulation. The present invention has the advantages of low cost, good rodenticidal killing effect, acute and chronic efficacy, good palatability, easy degradation, and the like.

The application relates to the technical field of rodent control, and discloses application of an avermectin and a rodenticide preparation, wherein the avermectin has a structure shown in formula (I). The application uses the strategy of 'new use of old drugs', uses marketable pesticides as candidate compounds, and uses mouse thrombin as a target to screen the pesticide avermectin which can strongly bind to thrombin, can activate the activity of mouse thrombin, and can promote blood clotting; the application has the advantages of low cost, good rodenticide effect, combination of acute and chronic, good palatability, easy degradation and the like.

This invention discloses an antithrombin III activity detection reagent, comprising a heparin diluent, a thrombin reagent, and a chromogenic substrate reagent. The heparin diluent includes heparin, a first buffer solution, EDTA, sodium chloride, and a preservative. The thrombin reagent includes thrombin, a second buffer solution, a protective agent, an inorganic salt, and a preservative. The chromogenic substrate reagent includes a chromogenic substrate and a preservative. This invention uses a chromogenic substrate method, which offers high sensitivity and accuracy, improves the stability of the thrombin reagent, and ensures the reagent's safety and stability, while also demonstrating good synergistic effects among its components.

This invention relates to a recombinant hirudin fusion protein with collagen affinity, its preparation method, and its applications. The fusion protein is a recombinant hirudin fusion protein incorporating a collagen-binding domain (CBD). The fusion protein of this invention possesses affinity for type I and type III collagen, and can inhibit thrombin activity to exert an anticoagulant effect. Furthermore, it can bind well to collagen-based composite materials, making it suitable for use in cardiovascular implants such as heart valves, cardiac occluders, dialysis membranes, oxygenation membranes, artificial blood vessels, and vascular stents, as well as in extracorporeal circulation and blood purification devices, enhancing anticoagulant and antithrombotic effects.