The invention discloses an electrochemical
luminescence biosensor based on a functionalized
nuclear track membrane. According to the electrochemical
luminescence biosensor, a
nuclear track membrane is used as a substrate, and terpyridyl
ruthenium functionalized silver nanoparticles are modified in nanometer pore channels of the
nuclear track membrane and are used as ECL illuminants; a gold nano layer is loaded on the outer surface of the nuclear track membrane through a spin-
coating method, and a trialkali matrix consisting of a
dopamine aptamer and an auxiliary chain containing a hydrophobic group is fixed. When the
dopamine is combined with the main chain of the
aptamer, the structure of the trialkali matrix is unwound to release an auxiliary chain, the hydrophobic barrier is eliminated, so that the nano pore channel is converted into an open state from a
closed state, and the material in the pore channel is in contact with a co-reactant tripropylamine (TPrA) in the
electrolyte to generate a remarkably enhanced ECL
signal. The
dopamine concentration is in a good
linear relationship with the ECL strength in a range of 0.1 nM to 10 mu M (R2 is equal to 0.995). According to the sensor, the interface stability is enhanced through the gold nanolayer, the recognition precision is improved through the
aptamer, the pore permeability is dynamically regulated and controlled through the hydrophobic group, and high-selectivity detection of dopamine is achieved.