Methods and apparatus are described that provide the ability to estimate the 
diffuse reflection component of an image of tissue such as 
skin captured without cross-polarization. It is thereby possible to estimate 
skin pigmentation information from an image of 
skin captured conventionally, such as, for example, a total reflection image, obtained in a conventional manner by shining 
white light on the skin and capturing the reflected light. The image may also be a partially 
diffuse reflection image, such as a low quality cross-polarized image. The 
diffuse reflection component of a captured image can then be further processed to obtain Red and Brown pigmentation images, useful for indicating the distribution of 
hemoglobin and 
melanin, the primary chromophores of skin. Additionally, a standard captured image of skin can be analyzed to obtain an estimate of the 
surface reflection component of the reflected light. The 
surface reflection component can then be used to generate a 
surface reflection image, useful for showing the distribution of light on the skin and to highlight superficial features such as wrinkles, fine lines, folds, pores, texture, and visible spots.