49 results about "Dna interaction" patented technology

A speech processing method and arrangement are described. A dynamic noise adaptation (DNA) model characterizes a speech input reflecting effects of background noise. A null noise DNA model characterizes the speech input based on reflecting a null noise mismatch condition. A DNA interaction model performs Bayesian model selection and re-weighting of the DNA model and the null noise DNA model to realize a modified DNA model characterizing the speech input for automatic speech recognition and compensating for noise to a varying degree depending on relative probabilities of the DNA model and the null noise DNA model.

According to particular exemplary aspects, DNA target site binding and cleavage properties of native, variant or modified homing endonucleases (HE) (e.g., LAGLIDAG (LHE), HNH, His-Cys Box, GIY-YIG, I-SspI-type, and fusions, muteins or variants thereof) in solution are recapitulated on the cell surface (e.g., as assessed by flow cytometric analysis) to provide for novel cells expressing one or more cell surface HEs (e.g., expressing one or more HE binding and / or cleavage specificities), novel cell libraries, and high-throughput methods for assessing target site binding, target site cleavage. The rapid analysis of HE and LHE-DNA interactions on the cell surface with concurrent sorting options provides for high-throughput library screening affording rapid identification, analysis and isolation of novel HEs or LHEs having novel sequence specificities. Such novel sequence specificities, obtained by said methods provide novel methods for introducing targeted DNA-strand cleavage events, and novel chromatin immunoprecipitation methods (CHIP methods).

The invention discloses a kit for detecting the interaction between protein and DNA, which at least includes exogenous histone antigen. Also disclosed is the application of the above kit in detecting the interaction of a small amount of protein and DNA, and the required sample amount can be as low as 1000 cells. The invention also discloses a method for detecting the interaction between protein and DNA and its application. The technical solution provided by the invention reduces sample loss and improves the efficiency of immunoprecipitation reaction by supplementing the antigen corresponding to the histone interacting with DNA to be studied in a small amount of initial samples. It has the following advantages: 1. The minimum required sample volume is 1000 cells, which is more suitable for the detection and analysis of the interaction between protein and DNA from a small number of samples; 2. ChIP experiments do not require specific kits and experimental equipment, and the cost is low. Has high universal applicability.

The invention belongs to the field of pharmaceutical chemistry, and particularly discloses 7-fluoro-substituted Isaindigotone derivatives. The structural formula of the derivatives is disclosed as Formula (I) or Formula (II), wherein X is C or N; R1 is hydrogen, amino, substituted amino, orpenta- or hexa-heterocyclic radical; R2 is hydrogen, hydroxy, amino, C1-C8 alkyl, or C1-C8 alkoxy or halo; R3, R5 and R6 are defined the same as R2; and R4 is hydrogen, hydroxy, amino, phenyl, C1-C8 cycloalkyl, C1-C8 alkyl, C1-C8 alkoxy, C1-C6 alkyl acyloxy, C1-C6 alkynyl, halo, or penta- or hexa-heterocyclic radical. The fluoro-substituted Isaindigotone derivatives can bebondedwith NM23-H2 protein and block the interaction between the NM23-H2 protein and c-myc G-quadruplex DNA, thereby causing the reduction of protooncogene c-myc transcription and translation, and inhibiting the proliferation of multiple tumor cell strains. The fluoro-substituted Isaindigotone derivatives have wide antitumor effects. The fluoro-substituted Isaindigotone derivatives are a novel NM23-H2-protein-targeted transcription regulation blocker, and have wide application space in preparing anticancer drugs.