58results about How to "Realize quantitative determination" patented technology

The invention discloses a magnetic particle chemiluminescence quantitative assay kit for anti-ribosomal P protein antibody IgG. The kit includes: anti-ribosomal P protein antibody IgG calibrator; Ribosomal P protein antigen and bovine serum albumin-labeled Tris buffer; alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin in Tris buffer; streptavidin-labeled magnetic particles and bovine serum albumin Serum albumin in Tris buffer; wash solution. Based on the traditional membrane strip immunoassay and enzyme-linked immunosorbent assay, the detection method of the kit increases the sensitivity and linear range by 3-5 orders of magnitude, and realizes quantitative detection in a real sense, with rapid response, reliable results, and It can be used in conjunction with a fully automatic chemiluminescence immunoassay analyzer to realize fully automatic use, and has irreplaceable important value for clinical diagnosis.

The invention provides a method for measuring the weathering depth of a rock. A microcrack and / or a microcrack zone produced on the inner side of a breakage surface after a rock is broken extend towards the inside of the rock by a perpendicular distance, and the perpendicular distance is the weathering depth. The method comprises the following steps of: collecting a rock sample according to a selected rock and / or a selected ore; cutting the prepared sample to be detected through a vertical section or a subvertical section; and quantitatively measuring the weathering depth of the rock by using a microscopic observation technology, a camera shooting technology and an image processing technology. The weathering depth is well defined by using microcrack zones which are distributed on the breakage surface of the rock and are found out for the first time, the weathering depth of the rock is quantitatively measured, the measuring method is easy to master, and a measuring result is accurate.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-histone antibody IgG. The kit comprises an anti-histone antibody IgG calibrator, an anti-histone antibody IgG quality control product, a Tris buffer containing biotin-labeled histone antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-double-stranded DNA antibody IgG. The kit comprises an anti-double-stranded DNA antibody IgG calibrator, an anti-double-stranded DNA antibody IgG quality control product, a Tris buffer containing biotin-labeled double-stranded DNA antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses a rare earth complex fluorescent probe, its preparation method and application in methanol impurity detection. The fluorescent probe is a compound of the following chemical formula: [Ln2(L)6(H2O)4].xH2O, wherein Ln is Tb or Eu, L as an electron-donating ligand, is salicylic acid or its derivative, and x ranges from 0 to 8. The preparation method consists of: dissolving the electron-donating ligand in water, adjusting the pH to 5-6.5, mixing the solution with a rare earth salt solution, standing or heating the obtained mixed solution till precipitation of a crystal, and filtering, washing, as well as drying the crystal so as to obtain the rare earth complex fluorescent probe. The binuclear complex synthesized in the invention as a fluorescent probe can effectively detect whether methanol is polluted by impurity solvents. And the detection method is simple, can be realized by employment of a relatively simple fluorometer, and needs no gas chromatograph and other complicated and expensive instruments, thus achieving the detection purposes of convenience and fastness.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-PM-Scl antibody IgG. The kit comprises an anti-PM-Scl antibody IgG calibrator, an anti-PM-Scl antibody IgG quality control product, a Tris buffer containing biotin-labeled PM-Scl antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses a gas phase chromatography-mass spectrometry detection method of polyoxyethylene nonyl phenyl ether in water, belonging to the technical field of water environment pollutant detection. The detection method comprises the steps of membrane filtration, pre-purification, solid phase extraction, enrichment and elution and comprises the following concrete steps: using an organicsolvent to extract the eluent which is specially treated chemically; then carrying out the gas phase chromatography-mass spectrometry detection of the organic phase; comparing the detection result with the standard curve to quantitatively obtain the concentration value of the polyoxyethylene nonyl phenyl ether. The invention has the advantages of simple pretreatment, easy operation, wide linear range, high enrichment factor, better reproducibility and accurate qualitative determination, has no need of adopting expensive liquid phase chromatography-mass spectrometry analyzers, and can quickly analyze the concentration of the polyoxyethylene nonyl phenyl ether in water.

The invention discloses a glow discharge-based plasma generator and a spectrum detection system formed by the glow discharge-based plasma generator; the spectrum detection system mainly comprises a sampling system, a power supply, the plasma generator, an optical detection system and a data processing system, wherein the plasma generator mainly comprises two discharge electrodes, and a working gas inlet cavity, a discharge cavity and a cushion chamber which are arranged in an insulation material generation device body and are sequentially communicated; the working gas inlet cavity has a pipe cavity structure; the side of the discharge cavity vertical to the working gas inlet direction has an open structure; the two discharge electrodes are oppositely arranged at the two sides along the other direction of the discharge cavity; the side of the cushion chamber facing the working gas inlet direction is a closed surface; the working gas enters through the gas inlet cavity, is ionized by the discharge cavity arranged between the two discharge electrodes and then is discharged outside from an opening of the discharge cavity; the glow discharge-based plasma generator is simple in structure, small in volume, low in power and low in gas consumption, and has the tendency of being developed into portable equipment.

The invention provides a detection method for oil sludge in oil used in electric power industry. The provided detection method can be used for quantitatively detecting the oil sludge content in oil, and comprises the steps of weighing two oil samples respectively, taking one oil sample for detecting pentane insolubles, and taking the other oil sample for detecting toluene insolubles, wherein the difference between the pentane insolubles and the toluene insolubles is oil sludge in oil. The detection method provided by the invention can be used for accurately detecting the oil sludge content in oil, and the change trend of the oil sludge in oil is clear at a glance during operation supervision, so that accurate judgment on the safe operation conditions of a unit can be given. The detection method can be used for overcoming the defect that an existing detection method can only qualitatively detect whether the oil sludge exists or not, and has the advantages of easiness and convenience in operation, obvious experimental phenomenon, simple operation process, and high accuracy.

The invention discloses cysteine hydrochloride in acetaminophen injection and a test method of degradation product cystine. The method adopts efficient liquid chromatography, a flowing phase adopts a phosphate buffer containing ion pairs and an organic solvent, and a gradient evaluation method is adopted. The method can be used for testing the content of cysteine hydrochloride in the acetaminophen injection, controlling the content of the content of cysteine degradation product cystine and testing the content of cysteine hydrochloride raw materials, the cysteine and the cystine have good resolution, the specificity and the sensitivity are high, and the operation is simple and convenient.

The invention discloses a method for measuring glucose by fluorescence spectrometry of enzyme-catalyzed oxidation TMB. The method comprises the following steps: (1) preparing a glucose standard solution system with known concentration, measuring the fluorescence peak intensity value at 380nm as F; (2) preparing a blank control solution system, and measuring the fluorescence peak intensity value asF0; (3) calculating the formula: deltaF=F-F0; (4) making a working curve for the concentration of glucose by deltaF; (5) preparing a measured sample solution, measuring the fluorescence peak intensity value as Fsample, and calculating the formula: deltaFsample=Fsample-F0; and (6) calculating the concentration of glucose in the measured sample according to the working curve. This method uses enzyme to catalyze the oxidation of TMB to produce a product with fluorescence, and uses the reaction product as a fluorescent molecular probe to linearly correlate the fluorescence peak intensity with theconcentration of the object under measurement, thereby realizing the quantitative determination of the target. The method is convenient, good in selectivity, and high in sensitivity.

The invention discloses a method for determining silicone oil in cosmetics, which comprises the following steps that a test sample is dispersed with water in a color comparison tube; after being dispersed, the test sample is transferred to a separating funnel with water to be fully shaken; silicone oil in the test sample is extracted with normal hexane; after standing, moisture is removed through filtering, and a filtrate is obtained; and then the content of the silicone oil in the filtrate is determined by using the Fourier transform infrared spectroscopy. According to the method, the test sample matrix is dispersed with the water, the silicone oil is fully extracted with the normal hexane, the pretreatment is easy and quick, the dissolving capacity of the normal hexane to the silicone oil is strong, and the self infrared light does not interfere with the quantification of the silicone oil.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-LKM-1 antibody IgG. The kit comprises an anti-LKM-1 antibody IgG calibrator, an anti-LKM-1 antibody IgG quality control product, a Tris buffer containing biotin-labeled LKM-1 antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention relates to a method for quantitatively determining micro-plastic in a water environment. The method comprises the following steps: filtering a constant-volume environment water sample byusing an enrichment membrane to entrap and enrich micro-plastic particles on the membrane, and enabling nano-plastic to enter filtrate; freezing and grinding the enrichment membrane enriched with themicro-plastic, adding distilled water to a constant volume, mixing, drying, and testing to finish the method for quantitatively determining the micro-plastic in the water environment. According to the present invention, the micro-plastic and the nano-plastic are separately determined for the first time, the micro-plastic (1 micrometer) separation and the quantitative determination at the microgramme / L level are achieved, and the method is successfully used in the actual water sample; and the method has the advantages of high sensitivity, high enrichment multiple, green and environment-friendly pretreatment process, and simple operation.

The invention provides a method for detecting a passivant in transformer oil by a high efficiency liquid chromatography. In the method, a high efficiency liquid chromatograph is adopted to detect and a chromatographic column with the length of 150 to 250mm and the column diameter of 4.6mm is selected, wherein silicon is used as a filler; a particle size is 5 micrometers; an aperture is 100 angstroms; a sample size is in the range of 10 to 20 microliters; a flow rate is 1 milliliter per minute; a column temperature is in the range of 30 to 35 DEG C; aqueous solution of methanol is used as a flowing phase; and an ultraviolet absorption detector is used as a detector. Due to the adoption of the method, the content of the passivant in the transformer oil can be detected, the sulfur attack of the transformer oil is evaluated, and the concentration level of the passivant in the oil is monitored and regulated.

The invention relates to a method for measuring and analyzing the content of acetone contained in power transformer oil for judging the insulating degree of a power transformer, adopting a static headspace gas chromatography method to measure the content of the acetone contained in the power transformer oil, i.e. a certain amount of transformer oil samples are added into a headspace bottle with certain volume to be heated and balanced under the temperature of 55 DEG C to 65 DEG C so that the acetone contained in the transformer oil samples is quantificationally vaporized, the content of the acetone contained in original oil samples is determined by measuring headspace gas inside a balance system, and then the information of an insulation and aging state of the inner part of the power transformer is analyzed and diagnosed.

The invention discloses a method and device for measuring coal wettability under high pressure through low-field nuclear magnetic resonance. The method comprises the following steps: performing low-field nuclear magnetic resonance measurement on water in pulverized coal under a standard condition to obtain a T2 spectrum of the water in the coal, and according to the position and the amplitude of afirst peak on the right side of the T2 spectrum, acquiring the geometric mean value T2g of the relaxation time T2 of the spectral peak; in combination with a measurement result theta of the water contact wetting angle of the conventional moulded coal surface in a parallel experiment, establishing a relational expression of a water wetting angle by using normalized T2g and NT2g: theta=84.376*NT2g+52.612; according to the relational expression, in combination with the T2g value of the water in the coal under a high-pressure condition, working out the water wetting angle of the coal under a setgas pressure. The device comprises a gas source, a sealed constant-pressure system and a nuclear magnetic magnet, wherein the nuclear magnetic magnet is connected with a low-field nuclear magnetic resonance instrument. According to the method, through establishment of a scaling relation between the coal wettability and a nuclear magnetic resonance T2 spectrum signal, quantitative determination ofthe coal wettability under different gas pressures is achieved; the method is new method for measuring the coal wettability instantly, in situ and dynamically.

The invention discloses a microelectrode CFME / CNT / ABTS + MBS for detecting HClO in trace blood and a preparation method and application thereof, and belongs to the technical field of application of organic molecular probes in biosensing. The preparation method comprises the following steps: firstly, synthesizing an electrochemical probe MBS with HClO specific recognition, and co-modifying MBS molecules and ABTS molecules with an internal reference effect on a CFME / CNT electrode through pi-pi bonds to prepare the HClO detection microelectrode CFME / CNT / ABTS + MBS. The microelectrode CFME / CNT / ABTS + MBS has high selectivity on the response of HClO, and the introduction of the internal reference also improves the detection accuracy and anti-pollution ability of an actual sample. The HClO microelectrode can realize qualitative or quantitative determination of HClO in a trace blood sample, and has a very good application prospect.

The invention discloses a method of measuring isocarbophos by the fluorescent spectrometry of aptamer mediating nitrogen-doped carbon spots catalyzing H2O2 reaction with TMB. The method comprises thefollowing steps that (1), an isocarbophos standard solution system with preset concentration is prepared; (2), a blank comparison solution system is prepared; (3), delta F=F-F0 is calculated; (4), thedelta F is used for conducting a working curve for the concentration of the isocarbophos; (5), a tested sample solution is prepared, and delta F sample=F sample-F0 is calculated; and (6), the isocarbophos concentration in the tested sample is calculated. The catalysis of isocarbophos aptamer reacting mediating nitrogen-doped carbon spots (CD) is adopted, that is, the CD catalyzes the oxidation ofTMB by H2O2 to produce fluorescence products. The CD is combined with the catalysis, and therefore sensitivity is greatly improved. The fluorescence products are used as a fluorescence probe and arein linear relationship with the concentration of the tested isocarbophos. Quantitative measuring of the isocarbophos by nitrogen-doped carbon spots catalytic reacting fluorescent spectrometry is realized. The method is simple and quick, is excellent in selectivity and is high in sensitivity.

The invention provides a liquid chromatography-mass spectrometry detection method for detecting nine acid azo colorants in animal dried aquatic products. The method comprises the following steps: carrying out homogenization, degreasing and extraction on an animal dried aquatic product sample; carrying out purification with a polyamide solid phase extraction column; and finally carrying out quantitative detection with a liquid chromatography-tandem quadrupole mass spectrometer. A C<18> chromatographic column is used for carrying out gradient elution with methanol and a 10mM ammonium acetate solution. A good linear range, satisfactory recovery and reproducibility are achieved for all the nine colorants. Moreover, the detection limit of the method is up to 0.1 to 1[mu]g / kg, and the quantitative limit is up to 0.25 to 25[mu]g / kg. The method has the advantages of easiness, convenience, rapidness, high recovery and high sensitivity, and is suitable for the detection of nine acid azo colorants in the animal dried aquatic products.

The invention discloses a magnetic particle chemiluminescence quantitative determination reagent kit of anti-centromere antibody IgG. The reagent kit comprises an anti-centromere antibody IgG calibration material, an anti-centromere antibody IgG quality control product, a Tris buffering solution containing biotin-labeled centromere antigens and bovine serum albumin, a Tris buffering solution containing alkaline phosphatase-labeled goat-anti-human polyclonal antibodies and bovine serum albumin, a Tris buffering solution containing streptavidin-labeled magnetic particles and bovine serum albumin, and a cleaning solution. According to a detection method of the reagent kit, on the basis of a traditional membrane strip immunization method and enzyme linked immunosorbent assay, sensitivity and the linear range are improved by 3-5 magnitude orders, quantitative determination in real sense is achieved, responding is fast, the result is reliable, and the magnetic particle chemiluminescence quantitative determination reagent kit can be full automatically used in cooperation with a full-automatic chemiluminescence immunity analyzer and has irreplaceable important value on clinical diagnosis.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-SLA / LP antibody IgG. The kit comprises an anti-SLA / LP antibody IgG calibrator, an anti-SLA / LP antibody IgG quality control product, a Tris buffer containing biotin-labeled SLA / LP antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses a time-varying radiation heat flow experiment system and measurement method based on distance control, and relates to an experiment device and a test system capable of realizing time-varying radiation heat flow (time-varying heat flow). The experiment system comprises a radiation source, a radiation source bearing frame, a radiation source moving bracket, a radiation sourcecontroller, a controller, a driver, a direct-current power supply and an experiment table base. The radiation source moving bracket adopts a guide rail sliding table. A guide rail sliding block is arranged on the guide rail sliding table. A radiation source bearing frame is installed on the guide rail sliding block, and a radiation source is fixedly installed below the radiation source bearing frame. A fixing frame is installed on the lower portion of the guide rail sliding block, a baffle rotating rod is installed on the fixing frame through a bolt, the baffle rotating rod is fixedly connected with a baffle, the baffle is located under the radiation source, and the motor is installed above the guide rail sliding table and drives the lead screw to rotate. The controller and the direct-current power supply are respectively connected with the driver, and the driver is connected with the motor. The direct-current power supply provides power for the motor. The radiation source controlleris connected with the radiation source.

The invention discloses a chemiluminescence immunoassay kit for quantitative detection of a soluble intercellular adhesion molecule-1 and a detection method. The kit contains a 96-well plate which is pre-coated with an anti-intercellular adhesion molecule-1 monoclonal antibody and the following bottled reagents: a phosphate wash solution, a blocking solution, a sample dilution solution, an intercellular adhesion molecule-1 standard solution, a horseradish peroxidase-labeled anti-intercellular adhesion molecule-1 polyclonal antibody IgG and a luminescent substrate, namely a luminal luminescent solution and quality control serum. The kit disclosed by the invention has the advantages of accurate and objective determination result of the soluble intercellular adhesion molecule-1 in human serum, simple and convenient method and easiness in popularization and application. The kit can reflect the autoimmune state in a patient with autoimmune thyroid disease can be used as a reference index for diagnosis of the autoimmune thyroid disease, and further has important significance for understanding the autoimmune active degree, predicting the occurrence of Graves disease and the recurrence of Graves hyperthyroidism, and determining reasonable treatment source, drug withdrawal time and other aspects.

The invention discloses a gas chromatography detecting method for the content of nonylphenol polyoxyethylene ether in a tanning agent, and relates to the field of analysis and testing. The method includes the steps of sample dissolution, chemical degradation treatment of sample liquid, purification by a solid-phase extraction column, gas chromatography detecting and comparison with a standard working curve to obtain the concentration value of nonylphenol polyoxyethylene ether. The method is convenient to operate, low in cost, high in reproducibility and accurate in qualitative process, and thecontent of nonylphenol polyoxyethylene ether in the tanning agent can be accurately detected without the aid of expensive gas chromatography-mass spectrometers or liquid chromatography-mass spectrometers. Compared with an existing testing technology, the method is low in cost and accurate and reliable in result when the concentration of nonylphenol polyoxyethylene ether in the tanning agent is detected.

The invention relates to a zinc ion detection method based on self-assembly nano particles of BODIPY dye micromolecules. The BODIPY dye micromolecules gather in a water solution medium and are self-assembled to form nano particles, thus, fluorescence quenching is caused, fluorescence is recovered through addition of zinc ions, the fluorescence intensity change of a product obtained after mixing within a certain time period at the 600 nm wavelength and the concentration of added zinc ions are in direct proportion, and accordingly, quantitative determination of the zinc ions is realized. The method is high in sensitivity, good in selectivity, simple to operate and capable of being used for determining zinc ions in a human hair sample.

The invention discloses a quick detection method for protein in milk. The method comprises the following steps: (1) preparing a sunset yellow solution as a detecting reagent; (2) preparing a series of standard protein aqueous solutions in concentrations, mixing the standard protein aqueous solutions with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, preparing a standard color gradation card according to the solution color or using an ultraviolet and visible spectrophotometer for determining absorbancy at 483nm and drawing a standard curve; (3) mixing a to-be-detected milk sample with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, comparing with the standard color gradation card or determining absorbancy at 483nm using the ultraviolet and visible spectrophotometer and calculating the content of the protein in the to-be-detected milk sample according to the standard curve. According to the method disclosed by the invention, the quick detection for the content of the protein in milk can be completed within 15-20min. When the method is adopted together with an ultraviolet spectrophotometer, the quantitative determination for the content of the protein in milk can be realized.

The invention discloses a method for detecting sildenafil in white spirit. The method comprises the steps of (1) carrying out spectral peak attribution on a Raman spectrum of sildenafil and obtaininga Raman characteristic peak of the sildenafil; (2) weighing a sildenafil standard, dissolving the sildenafil standard into methanol to obtain a sildenafil standard solution of which the concentrationis 1,000mg / L and mixing the white spirit with the sildenafil standard solution to prepare multiple test solutions of different concentrations; (3) mixing the test solutions with active colloid OTR 202at the volume ratio of 1:5 and carrying out Raman spectrum acquisition; and (4) establishing a linear regression equation, namely y=27.004x+20.516, wherein y is the content of the sildenafil in the white spirit, the unit is mg / L and x is the intensity of the Raman characteristic peak at 1401cm<-1>. The sildenafil in the white spirit is detected on the basis of surface-enhanced Raman spectroscopy,so that the method is novel, fast and accurate, and can meet the requirements on analysis and detection of the sildenafil in the white spirit.

The invention relates to an analysis method of a medicine, in particular to an analysis method of estriol. The analysis method of estriol adopts a hydrophilic reversed phase column; a moving phase consists of acetonitrile and water; and an eluting method adopts gradient elution. With the adoption of the method, related substances in a crude estriol product can be separated better.

The invention relates to a coal / shale adsorption capacity measuring device, which comprises an adsorption cylinder and a reference cylinder, wherein cylinder bottoms of the two cylinders are respectively fixed on the top surface and the bottom surface of a cylindrical steel block; the adsorption cylinder and the reference cylinder are communicated through a communicating pipe which penetrates through the two cylinder bottoms and the cylindrical steel block; a first control valve is arranged on the communicating pipe; outer side walls of the adsorption cylinder, the reference cylinder and the cylindrical steel block are provided with heating jackets, and heat insulation jackets are arranged at outer sides of the heating jackets; all the combined members, namely the adsorption cylinder, the reference cylinder, the cylindrical steel block, the heating jackets and the heat insulation jackets, are placed in a vacuum heat insulation tank; a sample hanging basket which is filled with a test sample is arranged in the adsorption cylinder; the adsorption cylinder is provided with an exhaust pipe and a first pressure sensor; and the reference cylinder is provided with an air inlet pipe and a second pressure sensor, wherein the air inlet pipe is connected to one end of a gas boosting pump, and the other end of the gas boosting pump is communicated with experiment gas and helium through a second control valve and a third control valve which are communicated in parallel. The coal / shale adsorption capacity measuring device can be used for simulating the process of adsorbing natural gas by coal / shale under actual geological conditions and evaluating the adsorption capacity of the coal / shale.