The invention discloses a quick detection method for
protein in milk. The method comprises the following steps: (1) preparing a
sunset yellow solution as a detecting
reagent; (2) preparing a series of standard
protein aqueous solutions in concentrations, mixing the standard
protein aqueous solutions with the detecting
reagent, oscillating, centrifuging, taking supernate and diluting with water, preparing a standard color
gradation card according to the solution color or using an
ultraviolet and visible spectrophotometer for determining absorbancy at 483nm and drawing a
standard curve; (3) mixing a to-be-detected
milk sample with the detecting
reagent, oscillating, centrifuging, taking supernate and diluting with water, comparing with the standard color
gradation card or determining absorbancy at 483nm using the
ultraviolet and visible spectrophotometer and calculating the content of the protein in the to-be-detected
milk sample according to the
standard curve. According to the method disclosed by the invention, the quick detection for the content of the protein in milk can be completed within 15-20min. When the method is adopted together with an
ultraviolet spectrophotometer, the
quantitative determination for the content of the protein in milk can be realized.