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Chemiluminescence immunoassay kit for quantitative detection of soluble intercellular adhesion molecule-1 and detection method

A technique of chemiluminescent immunity and adhesion molecules, which is applied in the fields of analytical materials, biological testing, biological material analysis, etc.

Inactive Publication Date: 2014-04-02
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention is to overcome the problems existing in imported kits and to solve the needs of further popularization and application, and to provide a kind of kit with high sensitivity, good specificity, easy operation and one-time operation. Chemiluminescent immunoassay detection kit and detection method for quantitative human serum soluble intercellular adhesion molecule-1 for the determination of a large number of samples

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  • Chemiluminescence immunoassay kit for quantitative detection of soluble intercellular adhesion molecule-1 and detection method
  • Chemiluminescence immunoassay kit for quantitative detection of soluble intercellular adhesion molecule-1 and detection method
  • Chemiluminescence immunoassay kit for quantitative detection of soluble intercellular adhesion molecule-1 and detection method

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0030] Main reagents and raw materials:

[0031] 1. Anti-ICAM-1 monoclonal antibody (prepared by ourselves)

[0032] 2. Carbonate buffer (prepared by yourself)

[0033] 3. Phosphate rinse solution (PBST) (prepared by yourself)

[0034] 4. Blocking solution (prepared by yourself)

[0035] 5. ICAM-1 standard product (manufactured by American R&D Company)

[0036] 6. Sample diluent (prepared by yourself)

[0037] 7. Horseradish peroxidase (HRP)-labeled anti-ICAM-1 polyclonal antibody IgG (i.e. enzyme-labeled secondary antibody) (prepared by ourselves)

[0038] 8. The luminescence substrate Luminal Luminescence Fluid (Beijing Keyue Zhongkai Biotechnology Co., Ltd., 250ml / bottle)

[0039] 9. Quality control serum (prepared by yourself)

[0040] 1. Preparation of anti-ICAM-1 monoclonal antibody

[0041] 1. Mix 10 μg of ICAM-1 protein with adjuvant a...

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Abstract

The invention discloses a chemiluminescence immunoassay kit for quantitative detection of a soluble intercellular adhesion molecule-1 and a detection method. The kit contains a 96-well plate which is pre-coated with an anti-intercellular adhesion molecule-1 monoclonal antibody and the following bottled reagents: a phosphate wash solution, a blocking solution, a sample dilution solution, an intercellular adhesion molecule-1 standard solution, a horseradish peroxidase-labeled anti-intercellular adhesion molecule-1 polyclonal antibody IgG and a luminescent substrate, namely a luminal luminescent solution and quality control serum. The kit disclosed by the invention has the advantages of accurate and objective determination result of the soluble intercellular adhesion molecule-1 in human serum, simple and convenient method and easiness in popularization and application. The kit can reflect the autoimmune state in a patient with autoimmune thyroid disease can be used as a reference index for diagnosis of the autoimmune thyroid disease, and further has important significance for understanding the autoimmune active degree, predicting the occurrence of Graves disease and the recurrence of Graves hyperthyroidism, and determining reasonable treatment source, drug withdrawal time and other aspects.

Description

technical field [0001] The invention belongs to chemiluminescence immunoassay method, specifically a chemiluminescence immunoassay ( CLIA) kits and detection methods. Background technique [0002] Autoimmune thyroid disease (autoimmune thyroid disease, AITD) is a group of organ-specific autoimmune diseases with complex pathogenesis, including Graves' disease (GD), Hashimoto's thyroiditis (Hashimoto's thyroiditis, HT), etc. . Human serum intercellular adhesion molecule-1 (ICAM-1) is a cell surface single-chain glycoprotein that is divided into an extracellular segment, a hydrophobic transmembrane segment and a short intracellular segment on the cell membrane. The extracellular segment has five extracellular immunoglobulin-like domains, of which the first immunoglobulin-like domain binds to the ligand of lymphocyte function-associated antigen-1 (LFA-1) , LFA-1 is expressed on all leukocytes, and the third structure-like region binds to complement receptor 3 (also known as M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803G01N33/542G01N2800/046
Inventor 柴锦燕李宁方佩华白庆双高硕谭建
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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