Chemiluminescence immunoassay kit for quantitative detection of soluble intercellular adhesion molecule-1 and detection method

A technique of chemiluminescent immunity and adhesion molecules, which is applied in the fields of analytical materials, biological testing, biological material analysis, etc.
CN103698537AInactive Publication Date: 2014-04-02GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
6 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Publication Date
2014-04-02
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a chemiluminescence immunoassay kit for quantitative detection of a soluble intercellular adhesion molecule-1 and a detection method. The kit contains a 96-well plate which is pre-coated with an anti-intercellular adhesion molecule-1 monoclonal antibody and the following bottled reagents: a phosphate wash solution, a blocking solution, a sample dilution solution, an intercellular adhesion molecule-1 standard solution, a horseradish peroxidase-labeled anti-intercellular adhesion molecule-1 polyclonal antibody IgG and a luminescent substrate, namely a luminal luminescent solution and quality control serum. The kit disclosed by the invention has the advantages of accurate and objective determination result of the soluble intercellular adhesion molecule-1 in human serum, simple and convenient method and easiness in popularization and application. The kit can reflect the autoimmune state in a patient with autoimmune thyroid disease can be used as a reference index for diagnosis of the autoimmune thyroid disease, and further has important significance for understanding the autoimmune active degree, predicting the occurrence of Graves disease and the recurrence of Graves hyperthyroidism, and determining reasonable treatment source, drug withdrawal time and other aspects.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to chemiluminescence immunoassay method, specifically a chemiluminescence immunoassay ( CLIA) kits and detection methods. Background technique

[0002] Autoimmune thyroid disease (autoimmune thyroid disease, AITD) is a group of organ-specific autoimmune diseases with complex pathogenesis, including Graves' disease (GD), Hashimoto's thyroiditis (Hashimoto's thyroiditis, HT), etc. . Human serum intercellular adhesion molecule-1 (ICAM-1) is a cell surface single-chain glycoprotein that is divided into an extracellular segment, a hydrophobic transmembrane segment and a short intracellular segment on the cell membrane. The extracellular segment has five extracellular immunoglobulin-like domains, of which the first immunoglobulin-like domain binds to the ligand of lymphocyte function-associated antigen-1 (LFA-1) , LFA-1 is expressed on all leukocytes, and the third structure-like region binds to complement receptor 3 (also known as M...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More