The invention discloses a mercury
ion detection
reagent and a detection method. The
reagent comprises nano
sol,
nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano
sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano
sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral
signal and carrying out
data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface
nucleic acid adaptors are changed, i.e. nano
particle aggregation in the silver sol and surface enhanced Roman scattering signals of
signal molecules are enhanced. In the invention, the SERS (Surface Enhanced
Raman Scattering) technology is adopted, the
detection limit to the Hg<2+> is at the nmol / L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and
high selectivity and is suitable for the quick detection of trace Hg<2+> in
environmental water.